EzCatDB

R L C P
Hierarchic Classification of Catalytic Mechanisms


R 1.-.-.- : Hydrolysis (bond cleavage by water) (Reaction)

L 1.15.-.- : Phosphoric Ester (Ligand group involved)

C 1.15.60000.- : Ribonuclease-U2-like mechanism (Base-Acid/Acid-Base/Stabilizer); 1st base activation of substrate-assisted nucleophile; covalent bond formation between scissile bond and substrate-assisted nucleophile (SN2) & 1st acid protonation to leaving group; Intermediate stabilization; 2nd base activation of water; Hydrolysis assisted by 2nd acid protonation to substrate-nucleophile (Catalytic mechanism)

P 1.15.60000.85 : His/Glu/Arg (Residues/cofactors in Protein)


1st Nucleophile : a group in substrate
Catalytic groups : groups in residue/substrate
General Base : a catalytic residue
General Acid : a catalytic residue

Related Enzymes

There are 2 entries in this class.
  • S00174 : 3.1.27.4; Ribonuclease U2 (Catalytic domain; 3.10.450.30)
  • S00175 : 3.1.27.10; Ribonuclease alpha-sarcin (Catalytic domain; 3.10.450.30)
  • Copyright: Nozomi Nagano, JST & CBRC-AIST
    Funded by PRESTO/Japan Science and Technology Agency (JST) (December 2001 - November 2004)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
    Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
    Funded by BIRD/Japan Science and Technology Agency (JST) (September 2005 - September 2008)
    Funded by BIRD/Japan Science and Technology Agency (JST) (October 2007 - September 2010)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2012 - March 2013)
    Supported by the commission for the Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI) (October 2012 - March 2016)
    Funded by the project commissioned by the New Energy and Industrial Technology Development Organization (NEDO) (April 2016 -)