RLCP 1.15.9400.1180 :EzCatDB EzCatDB

R L C P
Hierarchic Classification of Catalytic Mechanisms

R 1.-.-.- : Hydrolysis (bond cleavage by water) (Reaction)

L 1.15.-.- : Phosphoric Ester (Ligand group involved)

C 1.15.9400.- : Type-II restriction enzyme-like mechanism (Stabilizer); Two water molecules acting as base/acid; Substrate-assisted water activation for nucleophilic attack; Activated water stabilized; SN2-like nucleophilic attack by water; Cofactor-assisted stabilization of transition-state; Protonation to leaving group by another water bound to cofactor (Catalytic mechanism)

P 1.15.9400.1180 : Lys + two magnesium ions bound to acidic residues (Residues/cofactors in Protein)


1st Nucleophile : water
Catalytic groups : cofactor + groups in residue/substrate
General Base : a group in substrate/cofactor
General Acid : non-existent



Related Enzymes

There are 7 entries in this class.
  • S00383 : 3.1.21.4; Type-2 restriction enzyme Cfr10I (Catalytic domain; 3.40.91.10)
  • S00390 : 3.1.21.4; Type-2 restriction enzyme PvuII (Catalytic domain; 3.40.210.10)
  • S00403 : 3.1.21.4; Type-2 restriction enzyme EcoRI (Catalytic domain; 3.40.580.10)
  • S00404 : 3.1.21.4; Type-2 restriction enzyme EcoRV (Catalytic domain; 3.40.600.10)
  • S00405 : 3.1.21.4; Type-2 restriction enzyme BglI (Catalytic domain; 3.40.600.20)
  • S00462 : 3.1.11.3; Exonuclease (Catalytic domain; 3.90.320.10)
  • T00055 : 3.1.21.4; Type-2 restriction enzyme FokI (Catalytic domain; 3.40.91.30)