DB code: D00031

CATH domain 3.40.50.1770 : Rossmann fold Catalytic domain
3.40.50.720 : Rossmann fold Catalytic domain
E.C. 1.4.1.1
CSA 1pjb
M-CSA 1pjb
MACiE M0067

CATH domain Related DB codes (homologues)
3.40.50.720 : Rossmann fold S00543 S00551 S00552 S00553 S00602 S00604 S00605 S00608 S00610 S00625 S00319 S00328 S00329 S00330 S00331 S00332 D00456 D00457 D00458 S00324 S00320 S00325 S00326 S00327 D00459 S00335 S00336 S00334 T00219 S00339 D00513 D00001 D00002 D00003 D00005 D00007 D00008 D00010 D00012 D00017 D00018 D00023 D00027 D00028 D00032 D00033 D00034 D00035 D00037 D00048 D00071 D00476 D00481 D00482 D00490 D00492 D00494 D00545 D00601 D00603 D00604 D00605 D00615 D00845 D00857 D00858 M00161 M00171 M00210 T00002 T00010 T00011 T00015 T00227 T00247 T00408 T00414 D00827 D00262 D00274 D00275 M00035 T00109
3.40.50.1770 : Rossmann fold M00161

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
O52942 Alanine dehydrogenase
EC 1.4.1.1
PF01262 (AlaDh_PNT_C)
PF05222 (AlaDh_PNT_N)
[Graphical View]

KEGG enzyme name
alanine dehydrogenase
AlaDH
L-alanine dehydrogenase
NAD+-linked alanine dehydrogenase
alpha-alanine dehydrogenase
NAD+-dependent alanine dehydrogenase
alanine oxidoreductase
NADH-dependent alanine dehydrogenase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
O52942 O52942_PHOLP

KEGG Pathways
Map code Pathways E.C.
MAP00720 Reductive carboxylate cycle (CO2 fixation)
MAP00430 Taurine and hypotaurine metabolism

Compound table
Substrates Products Intermediates
KEGG-id C00041 C00001 C00003 C00022 C00014 C00004 C00080
E.C.
Compound L-Alanine H2O NAD+ Pyruvate NH3 NADH H+
Type amino acids H2O amide group,amine group,nucleotide carbohydrate,carboxyl group amine group,organic ion amide group,amine group,nucleotide others
ChEBI 16977
57972
15377
15846
32816
16134
16908
15378
PubChem 5950
7311724
22247451
962
5893
1060
222
439153
1038
1pjbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1pjcA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1sayA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:PYR Unbound Unbound
1pjbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1pjcA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAD Unbound Unbound Unbound
1sayA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
literature [2]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1pjbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 74;HIS 95;GLU 117
1pjcA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 74;HIS 95;GLU 117
1sayA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 74;HIS 95;GLU 117
1pjbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 269
1pjcA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 269
1sayA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 269

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[2]
p.563-564, Fig.3 3

References
[1]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 7896761
Journal J Biochem (Tokyo)
Year 1994
Volume 116
Pages 995-1000
Authors Sawa Y, Tani M, Murata K, Shibata H, Ochiai H
Title Purification and characterization of alanine dehydrogenase from a cyanobacterium, Phormidium lapideum.
Related PDB 1pjc
Related UniProtKB
[2]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9665169
Journal Nat Struct Biol
Year 1998
Volume 5
Pages 561-7
Authors Baker PJ, Sawa Y, Shibata H, Sedelnikova SE, Rice DW
Title Analysis of the structure and substrate binding of Phormidium lapideum alanine dehydrogenase.
Related PDB 1say 1pjb
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 10611473
Journal FEBS Lett
Year 1999
Volume 464
Pages 1-8
Authors Jackson JB, Peake SJ, White SA
Title Structure and mechanism of proton-translocating transhydrogenase.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 10385325
Journal Nat Biotechnol
Year 1999
Volume 17
Pages 588-92
Authors Hols P, Kleerebezem M, Schanck AN, Ferain T, Hugenholtz J, Delcour J, de Vos WM
Title Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 11041346
Journal Arch Microbiol
Year 2000
Volume 174
Pages 162-7
Authors Laue H, Cook AM
Title Purification, properties and primary structure of alanine dehydrogenase involved in taurine metabolism in the anaerobe Bilophila wadsworthia.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-03-16 2009-09-28