DB code: D00042

CATH domain 3.50.50.60 : FAD/NAD(P)-binding domain Catalytic domain
3.90.660.10 : Polyamine Oxidase; Chain A, domain 2 Catalytic domain
E.C. 1.5.3.14 1.5.3.15
CSA 1b5q
M-CSA 1b5q
MACiE

CATH domain Related DB codes (homologues)
3.90.660.10 : Polyamine Oxidase; Chain A, domain 2 T00211
3.50.50.60 : FAD/NAD(P)-binding domain M00163 D00015 D00041 D00045 D00064 D00071 T00004 T00015 T00017 T00025 T00211 T00213 T00233 T00242

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
O64411 Polyamine oxidase
EC 1.5.3.14
EC 1.5.3.15
NP_001105106.1 (Protein)
NM_001111636.1 (DNA/RNA sequence)
PF01593 (Amino_oxidase)
[Graphical View]

KEGG enzyme name
polyamine oxidase (propane-1,3-diamine-forming)
(EC 1.5.3.14 )
MPAO
(EC 1.5.3.14 )
maize PAO
(EC 1.5.3.14 )
N8-acetylspermidine oxidase (propane-1,3-diamine-forming)
(EC 1.5.3.15 )

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
O64411 PAO_MAIZE Spermidine + O(2) + H(2)O = propane-1,3-diamine + 4-aminobutanal +H(2)O(2). N(8)-acetylspermidine + O(2) + H(2)O = propane-1,3-diamine + 4-acetamidobutanal + H(2)O(2). Spermine + O(2) + H(2)O = N-(3-aminopropyl)-4-aminobutanal + trimethylenediamine + H(2)O(2). N(1)-acetylspermine + O(2) + H(2)O = N-(3-acetamidopropyl)-4-aminobutanal + trimethylenediamine + H(2)O(2). Monomer. FAD.

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00016 C00001 C00007 C00315 C01029 C00027 C00986 C00555 C05936
E.C. (1.5.3.14, 1.5.3.15)
(1.5.3.14, 1.5.3.15)
1.5.3.14
1.5.3.15
(1.5.3.14, 1.5.3.15)
(1.5.3.14, 1.5.3.15)
1.5.3.14
1.5.3.15
Compound FAD H2O O2 spermidine N8-acetylspermidine H2O2 propane-1,3-diamine 4-aminobutanal 4-acetamidobutanal
Type amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms),carbohydrate,nucleotide H2O others amine group,lipid amide group,amine group,lipid others amine group,lipid amine group,carbohydrate,lipid amide group,carbohydrate,lipid
ChEBI 16238
15377
15379
26689
27140
16610
27911
16240
15725
17769
7386
PubChem 643975
22247451
962
977
1102
123689
22326046
784
428
118
440850
1b37A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b37B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b37C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b5qA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b5qB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b5qC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h81A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h81B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h81C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h82A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h82B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h82C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h83A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h83B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h83C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h84A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h84B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h84C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h86A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h86B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h86C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:FAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b37A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b37B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b37C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b5qA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Analogue:MD2 Unbound Unbound Unbound Unbound Unbound
1b5qB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Analogue:MD2 Unbound Unbound Unbound Unbound Unbound
1b5qC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Analogue:MD2 Unbound Unbound Unbound Unbound Unbound
1h81A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h81B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h81C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1h82A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:GZZ Unbound Unbound Unbound Unbound Unbound Unbound
1h82B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:GZZ Unbound Unbound Unbound Unbound Unbound Unbound
1h82C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:GZZ Unbound Unbound Unbound Unbound Unbound Unbound
1h83A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Analogue:DIA Unbound Unbound Unbound
1h83B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Analogue:DIA Unbound Unbound Unbound
1h83C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Analogue:DIA Unbound Unbound Unbound
1h84A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:FAD-NBA
1h84B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:FAD-NBA
1h84C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:FAD-NBA
1h86A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:FAD-NBA
1h86B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:FAD-NBA
1h86C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:FAD-NBA

Reference for Active-site residues
resource references E.C.
literature [8]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1b37A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1b37B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1b37C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1b5qA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1b5qB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1b5qC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h81A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h81B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h81C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h82A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h82B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h82C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h83A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h83B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h83C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h84A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h84B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h84C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h86A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h86B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1h86C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 62
1b37A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1b37B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1b37C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1b5qA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1b5qB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1b5qC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h81A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h81B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h81C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h82A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h82B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h82C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h83A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h83B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h83C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h84A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h84B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h84C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h86A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h86B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170
1h86C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 170

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[6]
Fig.1(a), p.271-273
[8]
Figure 1 p.2767

References
[1]
Resource
Comments
Medline ID
PubMed ID 3181599
Journal Int J Biochem
Year 1988
Volume 20
Pages 695-702
Authors Tsukada T, Furusako S, Maekawa S, Hibasami H, Nakashima K
Title Purification by affinity chromatography and characterization of porcine liver cytoplasmic polyamine oxidase.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 1654929
Journal Biotechnol Appl Biochem
Year 1991
Volume 14
Pages 54-9
Authors Cona A, Federico R, Gramiccia M, Orsini S, Gradoni L
Title The amino aldehydes produced by spermine and spermidine oxidation with maize polyamine oxidase have anti-leishmanial effect.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 7503806
Journal Biochem Pharmacol
Year 1995
Volume 50
Pages 1527-30
Authors Libby PR, Munson BR, Fiel RJ, Porter CW
Title Cationic porphyrin derivatives as inhibitors of polyamine catabolism.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 9210657
Journal Arch Biochem Biophys
Year 1997
Volume 343
Pages 146-8
Authors Bellelli A, Angelini R, Laurenzi M, Federico R
Title Transient kinetics of polyamine oxidase from Zea mays L.
Related PDB
Related UniProtKB
[5]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS)
Medline ID 99192698
PubMed ID 10089528
Journal Acta Crystallogr D Biol Crystallogr
Year 1998
Volume 54
Pages 1429-31
Authors Binda C, Coda A, Angelini R, Federico R, Ascenzi P, Mattevi A
Title Crystallization and preliminary X-ray analysis of polyamine oxidase from Zea mays L.
Related PDB
Related UniProtKB O64411
[6]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)
Medline ID 99197292
PubMed ID 10368296
Journal Structure Fold Des
Year 1999
Volume 7
Pages 265-76
Authors Binda C, Coda A, Angelini R, Federico R, Ascenzi P, Mattevi A
Title A 30-angstrom-long U-shaped catalytic tunnel in the crystal structure of polyamine oxidase.
Related PDB 1b37 1b5q
Related UniProtKB O64411
[7]
Resource
Comments
Medline ID
PubMed ID 10999758
Journal Clin Cancer Res
Year 2000
Volume 6
Pages 3657-61
Authors Wallace HM, Duthie J, Evans DM, Lamond S, Nicoll KM, Heys SD
Title Alterations in polyamine catabolic enzymes in human breast cancer tissue.
Related PDB
Related UniProtKB
[8]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 11258887
Journal Biochemistry
Year 2001
Volume 40
Pages 2766-76
Authors Binda C, Angelini R, Federico R, Ascenzi P, Mattevi A
Title Structural bases for inhibitor binding and catalysis in polyamine oxidase.
Related PDB 1h81 1h82 1h83 1h84 1h86
Related UniProtKB

Comments
According to the literature [6] and [8], this enzyme catalyzes the following reactions:
(A) Hydride transfer from N1-acetylspermine to FAD, giving an imino intermediate and FADH2:
(B) Exchange of double-bonded atoms of the imino intermediate by water, giving N1-acetylspermidine and 3-aminopropanal:
(C) Hydride transfer from FADH2 to O2, giving FAD and H2O2:

Created Updated
2004-06-03 2012-10-03