DB code: D00079

RLCP classification 3.747.6300.267 : Transfer
CATH domain 3.40.50.150 : Rossmann fold Catalytic domain
1.10.1020.10 : Adenine-specific Methyltransferase; domain 2
E.C. 2.1.1.72
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.40.50.150 : Rossmann fold S00637 S00639 S00262 S00261 S00291 S00412 D00075 D00076 D00080 D00082 D00083 D00823

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
P04043 Modification methylase DpnIIA
M.DpnIIA
EC 2.1.1.72
Adenine-specific methyltransferase DpnIIA
M.DpnII 1
PF02086 (MethyltransfD12)
[Graphical View]

KEGG enzyme name
site-specific DNA-methyltransferase (adenine-specific)
modification methylase
restriction-modification system

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P04043 MTD21_STRPN S-adenosyl-L-methionine + DNA adenine = S- adenosyl-L-homocysteine + DNA 6-methylaminopurine. Homodimer.

KEGG Pathways
Map code Pathways E.C.

Compound table
Substrates Products Intermediates
KEGG-id C00019 C00821 C00021 C03391
E.C.
Compound S-Adenosyl-L-methionine DNA adenine S-Adenosyl-L-homocysteine DNA 6-methylaminopurine
Type amino acids,amine group,nucleoside,sulfonium ion amine group,nucleic acids amino acids,amine group,nucleoside,sulfide group amine group,nucleic acids
ChEBI 67040
16680
57856
PubChem 34755
25246222
439155
2dpmA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:SAM Unbound Unbound Unbound
2dpmA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
2dpmA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 194
2dpmA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[1]
p.1572-1573

References
[1]
Resource
Comments
Medline ID
PubMed ID 9862809
Journal Structure
Year 1998
Volume 6
Pages 1563-75
Authors Tran PH, Korszun ZR, Cerritelli S, Springhorn SS, Lacks SA
Title Crystal structure of the DpnM DNA adenine methyltransferase from the DpnII restriction system of streptococcus pneumoniae bound to S-adenosylmethionine.
Related PDB 2dpm
Related UniProtKB P04043

Comments
This methylase recognizes the double-stranded sequence [G-A-T-C], causes specific methylation on A-2, and protects the DNA from cleavage by the DpnII endonuclease.
According to the literature [1], the catalysis of methyl transfer directly to adenine N6 is presumably mediated by the negative polarization of N6 by hydrogen bonding to Asp194 and Pro195.

Created Updated
2002-08-14 2009-02-26