DB code: D00091

CATH domain 3.30.70.250 : Alpha-Beta Plaits
3.40.366.10 : Malonyl-Coenzyme A Acyl Carrier Protein; domain 2 Catalytic domain
E.C. 2.3.1.39
CSA 1mla
M-CSA 1mla
MACiE M0291

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P0AAI9 Malonyl CoA-acyl carrier protein transacylase
MCT
EC 2.3.1.39
NP_415610.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_489360.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF00698 (Acyl_transf_1)
[Graphical View]

KEGG enzyme name
[acyl-carrier-protein] S-malonyltransferase
[acyl carrier protein]malonyltransferase
FabD
malonyl coenzyme A-acyl carrier protein transacylase
malonyl transacylase
malonyl transferase
malonyl-CoA-acyl carrier protein transacylase
malonyl-CoA:[acyl-carrier-protein] S-malonyltransferase
malonyl-CoA:ACP transacylase
malonyl-CoA:ACP-SH transacylase
malonyl-CoA:AcpM transacylase
malonyl-CoA:acyl carrier protein transacylase
malonyl-CoA:acyl-carrier-protein transacylase
malonyl-CoA/dephospho-CoA acyltransferase
MAT
MCAT
MdcH

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P0AAI9 FABD_ECOLI Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].

KEGG Pathways
Map code Pathways E.C.
MAP00061 Fatty acid biosynthesis

Compound table
Substrates Products Intermediates
KEGG-id C00083 C00229 C00010 C01209
E.C.
Compound Malonyl-CoA Acyl-carrier protein CoA Malonyl-[acyl-carrier protein]
Type amine group,carbohydrate,carboxyl group,nucleotide ,peptide/protein,sulfide group carbohydrate,peptide/protein,phosphate group/phosphate ion,sulfhydryl group amine group,carbohydrate,nucleotide ,peptide/protein,sulfhydryl group carbohydrate,carboxyl group,peptide/protein,phosphate group/phosphate ion,sulfide group
ChEBI 15531
15346
PubChem 10663
644066
6816
87642
1mlaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1mlaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
PDB;1mla & Swiss-prot;P0AAI9

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1mlaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1mlaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 92;HIS 201

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[2]
p.12962-12964

References
[1]
Resource
Comments
Medline ID
PubMed ID 8078074
Journal J Mol Biol
Year 1994
Volume 242
Pages 99-102
Authors Serre L, Swenson L, Green R, Wei Y, Verwoert II, Verbree EC, Stuitje AR, Derewenda ZS
Title Crystallization of the malonyl coenzyme A-acyl carrier protein transacylase from Escherichia coli.
Related PDB
Related UniProtKB
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS)
Medline ID 95286570
PubMed ID 7768883
Journal J Biol Chem
Year 1995
Volume 270
Pages 12961-4
Authors Serre L, Verbree EC, Dauter Z, Stuitje AR, Derewenda ZS
Title The Escherichia coli malonyl-CoA:acyl carrier protein transacylase at 1.5-A resolution. Crystal structure of a fatty acid synthase component.
Related PDB 1mla
Related UniProtKB P0AAI9
[3]
Resource
Comments
Medline ID
PubMed ID 9215574
Journal Protein Eng
Year 1997
Volume 10
Pages 561-6
Authors Rangan VS, Serre L, Witkowska HE, Bari A, Smith S
Title Characterization of the malonyl-/acetyltransacylase domain of the multifunctional animal fatty acid synthase by expression in Escherichia coli and refolding in vitro.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 9862793
Journal Chem Biol
Year 1998
Volume 5
Pages 699-711
Authors Matharu AL, Cox RJ, Crosby J, Byrom KJ, Simpson TJ
Title MCAT is not required for in vitro polyketide synthesis in a minimal actinorhodin polyketide synthase from Streptomyces coelicolor.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 9632638
Journal J Biol Chem
Year 1998
Volume 273
Pages 15920-6
Authors Wilson MC, Jackson VN, Heddle C, Price NT, Pilegaard H, Juel C, Bonen A, Montgomery I, Hutter OF, Halestrap AP
Title Lactic acid efflux from white skeletal muscle is catalyzed by the monocarboxylate transporter isoform MCT3.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 10421763
Journal Chem Biol
Year 1999
Volume 6
Pages 577-84
Authors Zhou P, Florova G, Reynolds KA
Title Polyketide synthase acyl carrier protein (ACP) as a substrate and a catalyst for malonyl ACP biosynthesis.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 10684659
Journal Biochemistry
Year 2000
Volume 39
Pages 2088-95
Authors Dreier J, Khosla C
Title Mechanistic analysis of a type II polyketide synthase. Role of conserved residues in the beta-ketoacyl synthase-chain length factor heterodimer.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 11814333
Journal Biochemistry
Year 2002
Volume 41
Pages 1421-7
Authors Szafranska AE, Hitchman TS, Cox RJ, Crosby J, Simpson TJ
Title Kinetic and mechanistic analysis of the malonyl CoA:ACP transacylase from Streptomyces coelicolor indicates a single catalytically competent serine nucleophile at the active site.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-03-17 2012-06-01