DB code: D00106

RLCP classification 6.20.93000.5520 : Double-bonded atom exchange
6.30.115020.5300 : Double-bonded atom exchange
CATH domain 3.30.470.10 : D-amino Acid Aminotransferase; Chain A, domain 1
3.20.10.10 : D-amino Acid Aminotransferase; Chain A, domain 2 Catalytic domain
E.C. 2.6.1.42
CSA 1iyd
M-CSA 1iyd
MACiE

CATH domain Related DB codes (homologues)
3.30.470.10 : D-amino Acid Aminotransferase; Chain A, domain 1 D00105
3.20.10.10 : D-amino Acid Aminotransferase; Chain A, domain 2 D00105

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P0AB80 Branched-chain-amino-acid aminotransferase
BCAT
EC 2.6.1.42
Transaminase B
YP_026247.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491668.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF01063 (Aminotran_4)
[Graphical View]
O15382 Branched-chain-amino-acid aminotransferase, mitochondrial
BCAT(m)
EC 2.6.1.42
Placental protein 18
PP18
NP_001158245.1 (Protein)
NM_001164773.1 (DNA/RNA sequence)
NP_001181.2 (Protein)
NM_001190.3 (DNA/RNA sequence)
PF01063 (Aminotran_4)
[Graphical View]

KEGG enzyme name
branched-chain-amino-acid transaminase
transaminase B
branched-chain amino acid aminotransferase
branched-chain amino acid-glutamate transaminase
branched-chain aminotransferase
L-branched chain amino acid aminotransferase
glutamate-branched-chain amino acid transaminase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
O15382 BCAT2_HUMAN L-leucine + 2-oxoglutarate = 4-methyl-2- oxopentanoate + L-glutamate. 2-oxoglutaric acid + L-isoleucine = (S)-3- methyl-2-oxopentanoic acid + L-glutamic acid. 2-oxoglutaric acid + L-valine = 3-methyl-2- oxobutanoic acid + L-glutamic acid. Homodimer. Isoform A: Mitochondrion. Isoform B: Cytoplasm. Pyridoxal phosphate.
P0AB80 ILVE_ECOLI L-leucine + 2-oxoglutarate = 4-methyl-2- oxopentanoate + L-glutamate. 2-oxoglutaric acid + L-isoleucine = (S)-3- methyl-2-oxopentanoic acid + L-glutamic acid. 2-oxoglutaric acid + L-valine = 3-methyl-2- oxobutanoic acid + L-glutamic acid. Homohexamer. Pyridoxal phosphate.

KEGG Pathways
Map code Pathways E.C.
MAP00280 Valine, leucine and isoleucine degradation
MAP00290 Valine, leucine and isoleucine biosynthesis
MAP00770 Pantothenate and CoA biosynthesis

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00018 C00123 C00407 C00183 C00026 C00233 C03465 C00141 C00025
E.C.
Compound Pyridoxal phosphate L-Leucine L-Isoleucine L-Valine 2-Oxoglutarate 4-Methyl-2-oxopentanoate 3-Methyl-2-oxopentanoate 3-Methyl-2-oxobutanoate L-Glutamate External aldimine Quinonoid intermediate Ketimine intermediate Carbinolamine intermediate
Type aromatic ring (with nitrogen atoms),phosphate group/phosphate ion amino acids amino acids amino acids carbohydrate,carboxyl group carbohydrate,carboxyl group carbohydrate,carboxyl group carbohydrate,carboxyl group amino acids,carboxyl group
ChEBI 18405
57427
15603
58045
17191
57762
16414
30915
48430
35932
16530
16015
PubChem 1051
7045798
6106
7043901
6306
88733505
6971018
6287
51
70
47
49
88747398
44272391
33032
1a3gA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a3gB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a3gC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1kA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1kB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1kC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1lA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1lB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1lC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1mA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1mB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1mC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1iydA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Analogue:GUA Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1iydB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Analogue:GUA Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1iydC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Analogue:GUA Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1iyeA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1iyeB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1iyeC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ekfA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ekfB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ekpA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ekpB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ekvA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ekvB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1kt8A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1kt8B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ktaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ktaB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a3gA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a3gB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a3gC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1kA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1kB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1kC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1lA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:PLP-2ML Unbound Unbound Unbound
1i1lB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:PLP-2ML Unbound Unbound Unbound
1i1lC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:PLP-2ML Unbound Unbound Unbound
1i1mA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Analogue:4MV Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1mB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Analogue:4MV Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i1mC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Analogue:4MV Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1iydA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1iydB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1iydC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1iyeA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:PGU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:PGU Unbound Unbound
1iyeB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:PGU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:PGU Unbound Unbound
1iyeC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:PGU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:PGU Unbound Unbound
1ekfA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ekfB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ekpA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ekpB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ekvA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:PLP-TRS
1ekvB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:PLP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:PLP-TRS
1kt8A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:ILP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-bound:ILP Unbound
1kt8B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:ILP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-bound:ILP Unbound
1ktaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:PMP Unbound Unbound Unbound Analogue:KIV Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ktaB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:PMP Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1a3gA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1a3gB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1a3gC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i1kA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i1kB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i1kC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i1lA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i1lB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i1lC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i1mA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i1mB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i1mC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1iydA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1iydB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1iydC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1iyeA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1iyeB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1iyeC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ekfA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ekfB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ekpA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ekpB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ekvA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ekvB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kt8A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kt8B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ktaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ktaB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1a3gA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1a3gB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1a3gC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1i1kA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1i1kB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 659;TYR 664 LYS 659(PLP binding)
1i1kC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 1159;TYR 1164 LYS 1159(PLP binding)
1i1lA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1i1lB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 659;TYR 664 LYS 659(PLP binding)
1i1lC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 1159;TYR 1164 LYS 1159(PLP binding)
1i1mA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1i1mB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 659;TYR 664 LYS 659(PLP binding)
1i1mC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 1159;TYR 1164 LYS 1159(PLP binding)
1iydA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1iydB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1iydC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1iyeA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1iyeB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1iyeC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 159;TYR 164 LYS 159(PLP binding)
1ekfA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 202;TYR 207 LYS 202(PLP binding)
1ekfB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 202;TYR 207 LYS 202(PLP binding)
1ekpA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 202;TYR 207 LYS 202(PLP binding)
1ekpB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 202;TYR 207 LYS 202(PLP binding)
1ekvA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 202;TYR 207 LYS 202(PLP binding)
1ekvB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 202;TYR 207 LYS 202(PLP binding)
1kt8A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 202;TYR 207 LYS 202(PLP binding)
1kt8B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 702;TYR 707 LYS 702(PLP binding)
1ktaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 202;TYR 207 LYS 202(PLP binding)
1ktaB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 702;TYR 707 LYS 702(PLP binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[5]
p.111-112
[6]
p.7459-7460
[9]
p.11594-11599
[10]
Scheme 2, Fig.7, p.3728-3730, p.3731-3733

References
[1]
Resource
Comments
Medline ID
PubMed ID 2666406
Journal J Biochem (Tokyo)
Year 1989
Volume 105
Pages 671-2
Authors Kamitori S, Odagaki Y, Inoue K, Kuramitsu S, Kagamiyama H, Matsuura Y, Higuchi T
Title Crystallization and preliminary X-ray characterization of branched-chain amino acid aminotransferase from Escherichia coli.
Related PDB
Related UniProtKB
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)
Medline ID 97306043
PubMed ID 9163511
Journal J Biochem (Tokyo)
Year 1997
Volume 121
Pages 637-41
Authors Okada K, Hirotsu K, Sato M, Hayashi H, Kagamiyama H
Title Three-dimensional structure of Escherichia coli branched-chain amino acid aminotransferase at 2.5 A resolution.
Related PDB 1a3g
Related UniProtKB P0AB80
[3]
Resource
Comments
Medline ID
PubMed ID 9914259
Journal Curr Opin Struct Biol
Year 1998
Volume 8
Pages 759-69
Authors Jansonius JN
Title Structure, evolution and action of vitamin B6-dependent enzymes.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 10989422
Journal Methods Enzymol
Year 2000
Volume 324
Pages 103-13
Authors Kagamiyama H, Hayashi H
Title Branched-chain amino-acid aminotransferase of Escherichia coli.
Related PDB
Related UniProtKB
[5]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 11264579
Journal Acta Crystallogr D Biol Crystallogr
Year 2001
Volume 57
Pages 506-15
Authors Yennawar N, Dunbar J, Conway M, Hutson S, Farber G
Title The structure of human mitochondrial branched-chain aminotransferase.
Related PDB 1ekf 1ekp 1ekv
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 11412098
Journal Biochemistry
Year 2001
Volume 40
Pages 7453-63
Authors Okada K, Hirotsu K, Hayashi H, Kagamiyama H
Title Structures of Escherichia coli branched-chain amino acid aminotransferase and its complexes with 4-methylvalerate and 2-methylleucine: induced fit and substrate recognition of the enzyme.
Related PDB 1i1k 1i1l 1i1m
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 11642362
Journal Prog Nucleic Acid Res Mol Biol
Year 2001
Volume 70
Pages 175-206
Authors Hutson S
Title Structure and function of branched chain aminotransferases.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 12119021
Journal Biochemistry
Year 2002
Volume 41
Pages 9070-8
Authors Conway ME, Yennawar N, Wallin R, Poole LB, Hutson SM
Title Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 12269802
Journal Biochemistry
Year 2002
Volume 41
Pages 11592-601
Authors Yennawar NH, Conway ME, Yennawar HP, Farber GK, Hutson SM
Title Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: ketimine and pyridoxamine phosphate forms.
Related PDB 1kt8 1kta
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 12667063
Journal Biochemistry
Year 2003
Volume 42
Pages 3725-33
Authors Goto M, Miyahara I, Hayashi H, Kagamiyama H, Hirotsu K
Title Crystal structures of branched-chain amino acid aminotransferase complexed with glutamate and glutarate: true reaction intermediate and double substrate recognition of the enzyme.
Related PDB 1iyd 1iye
Related UniProtKB

Comments
This enzyme belongs to Type-VI PLP-dependent enzyme (see [3] & [5]).
According to the literature [10], this enzyme catalyzes the following reactions.
(A) Formation of external aldimine (with amine group of the first substrate, Leucine).
(A1) The negative charges from phosphate group of PLP and alpha-carboxylate of the first substrate may deprotonate the nucleophile, the alpha-amino group of the substrate (see [10]).
(A2) The activated alpha-amino group of the first substrate makes a nucleophilic attack on the C4' atom of PLP (from the si-face side), forming a tetrahedral intermediate.
(A3) Probably, the lone pair of the substituted amino group makes another attack on the C4' atom of PLP (from the si-face side), releasing Lys159. This may facilitated by O3 atom of PLP and Tyr164, considering the structure (see [10]). (The O3 atom might act as a acid-base to transfer a proton from the amine group to sidechain of Lys159.)
(B) Isomerization (change in the position of double-bond).
(B1) Lys159 acts as a general base to deprotonate the alpha-proton of the substrate bound to PLP, forming the quinonoid intermediate. The activity of Lys159 must be modulated by Tyr164 & O3 atom of PLP.
(B2) Lys159 acts as a general acid to protonate the C4' atom of the intermediate, forming the ketimine intermediate.
(C) Schiff-base deforming (by hydration), releasing the first product, 2-oxo acid, and PMP.
(C1) Lys159 acts as a general base to activate a water (at the re-face side of cofactor).
(C2) The activated water molecule makes a nucleophilic attack on the C-alpha atom of the substrate (from the re-face side), forming a carbinolamine intermediate.
(C3) The lone pair of the new hydroxyl group makes a nucleophilic attack on the C4' atom, releasing the first product. This reaction may facilitated by Tyr164 and the O3 atom of PLP. (The O3 atom might act as a base to deprotonate the sidechain of Lys159, which in turn deprotonate the hydroxyl group. The O3 atom then acts as a acid to protonate to the leaving amino group of the intermediate.)
(D) Schiff-base forming (of PMP with carbonyl group of the second substrate, Oxoglutarate; by dehydration).
(E) Isomerization (change in the position of double-bond).
(F) Formation of internal aldimine, releasing the second product, Glutamate.

Created Updated
2004-03-18 2009-02-26