DB code: D00143

CATH domain 1.20.90.10 : Phospholipase A2 Catalytic domain
4.10.410.10 : Factor Xa Inhibitor
E.C. 3.1.1.4
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
P00617 Basic phospholipase A2 beta-bungarotoxin A1 chain
Beta-BuTX A1 chain
svPLA2
EC 3.1.1.4
Phosphatidylcholine 2-acylhydrolase
PF00068 (Phospholip_A2_1)
[Graphical View]
P00989 Beta-bungarotoxin B2 chain
Beta-2-bungarotoxin
PF00014 (Kunitz_BPTI)
[Graphical View]

KEGG enzyme name
phospholipase A2
lecithinase A
phosphatidase
phosphatidolipase
phospholipase A

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P00989 IVB2_BUNMU Heterodimer, disulfide-linked. The A chains have phospholipase A2 activity and the B chains show homology with the basic protease inhibitors. Secreted.
P00617 PA21B_BUNMU Phosphatidylcholine + H(2)O = 1- acylglycerophosphocholine + a carboxylate. Heterodimer, disulfide-linked. The A chains have phospholipase A2 activity and the B chains show homology with the basic protease inhibitors. The A1 chain is found in beta-1 and beta-2 bungarotoxins. Secreted. Binds 1 calcium ion (By similarity).

KEGG Pathways
Map code Pathways E.C.
MAP00564 Glycerophospholipid metabolism
MAP00565 Ether lipid metabolism
MAP00590 Arachidonic acid metabolism
MAP00591 Linoleic acid metabolism
MAP00592 alpha-Linolenic acid metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00076 C00157 C00001 C00060 C04230
E.C.
Compound Calcium phosphatidylcholine H2O Carboxylate 1-Acyl-sn-glycero-3-phosphocholine
Type divalent metal (Ca2+, Mg2+) amine group,carbohydrate,lipid,phosphate group/phosphate ion H2O carboxyl group amine group,carbohydrate,lipid,phosphate group/phosphate ion
ChEBI 29108
15377
PubChem 271
22247451
962
1bunA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_NA_87 Unbound Unbound Unbound
1bunB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P00617

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1bunA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 48;ASP 94 TYR 28;GLY 30;GLY 32;ASP 49(Calcium binding)
1bunB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis

References
[1]
Resource
Comments CHARACTERIZATION OF PRESYNAPTIC NEUROTOXINS.
Medline ID 78043174
PubMed ID 303565
Journal Eur J Biochem
Year 1977
Volume 80
Pages 1-12
Authors Abe T, Alema S, Miledi R
Title Isolation and characterization of presynaptically acting neurotoxins from the venom of Bungarus snakes.
Related PDB
Related UniProtKB P00617
[2]
Resource
Comments CHARACTERIZATION OF PHOSPHOLIPASE A2 ACTIVITY.
Medline ID 79088714
PubMed ID 730754
Journal J Biochem (Tokyo)
Year 1978
Volume 84
Pages 1301-8
Authors Kondo K, Toda H, Narita K
Title Characterization of phospholipase A activity of beta1-bungarotoxin from Bungarus multicinctus venom. II. Identification of the histidine residue of beta1-bungarotoxin modified by p-bromophenacyl bromide.
Related PDB
Related UniProtKB P00617
[3]
Resource
Comments
Medline ID
PubMed ID 2590165
Journal Biochem J
Year 1989
Volume 262
Pages 773-9
Authors Chu ST, Chen YH
Title The intrinsic tryptophan fluorescence of beta 1-bungarotoxin and the Ca2+-binding domains of the toxin as probed with Tb3+ luminescence.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 1898340
Journal Biochem J
Year 1991
Volume 278
Pages 481-6
Authors Chu ST, Chen YH
Title Role of the N-terminal region of phospholipase A2 subunit of beta 1-bungarotoxin in the toxin-Ca2+ complex-formation.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 1485347
Journal Toxicon
Year 1992
Volume 30
Pages 1501-4
Authors Shina R, Rosenberg P, Condrea E
Title An EDTA.Ca2+ complex inhibits the enzymatic activity but not the lethality of beta-bungarotoxin.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 8240282
Journal Biochem J
Year 1993
Volume 295
Pages 713-8
Authors Chu ST, Chu CC, Tseng CC, Chen YH
Title Met-8 of the beta 1-bungarotoxin phospholipase A2 subunit is essential for the phospholipase A2-independent neurotoxic effect.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 7702746
Journal J Protein Chem
Year 1994
Volume 13
Pages 641-8
Authors Chang LS, Kuo KW, Lin SR, Chang CC
Title Functional involvement of Lys-6 in the enzymatic activity of phospholipase A2 from Bungarus multicinctus (Taiwan banded krait) snake venom.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 8060495
Journal J Protein Chem
Year 1994
Volume 13
Pages 233-6
Authors Chang LS, Lin SR, Chang CC, Yang CC
Title The essentiality of B chain in stabilizing the structure of the A chain in beta 1-bungarotoxin from Bungarus multicinctus venom.
Related PDB
Related UniProtKB
[9]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8590005
Journal Structure
Year 1995
Volume 3
Pages 1109-19
Authors Kwong PD, McDonald NQ, Sigler PB, Hendrickson WA
Title Structure of beta 2-bungarotoxin: potassium channel binding by Kunitz modules and targeted phospholipase action.
Related PDB 1bun
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 8829622
Journal Biochem Mol Biol Int
Year 1996
Volume 38
Pages 617-23
Authors Chang LS, Wen EY, Chang CC
Title The structural elements of phospholipase A2 affecting the enhancement of 8-anilinonaphthalene-1-sulfonate fluorescence.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 9161720
Journal Biochem Mol Biol Int
Year 1997
Volume 41
Pages 1247-53
Authors Chang LS, Chang CC, Wu PF
Title The structural and functional essentiality of the N-terminal alpha-helix in the phospholipase A2 of the Taiwan banded krait.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 11286555
Journal J Mol Biol
Year 2001
Volume 307
Pages 1049-59
Authors Singh G, Gourinath S, Sharma S, Paramasivam M, Srinivasan A, Singh TP
Title Sequence and crystal structure determination of a basic phospholipase A2 from common krait (Bungarus caeruleus) at 2.4 A resolution: identification and characterization of its pharmacological sites.
Related PDB
Related UniProtKB
[13]
Resource
Comments REVIEW.
Medline ID 20396379
PubMed ID 10936627
Journal Toxicon
Year 2001
Volume 39
Pages 107-18
Authors Rowan EG
Title What does beta-bungarotoxin do at the neuromuscular junction?
Related PDB
Related UniProtKB P00617 P00989

Comments
This protein, beta-bungarotoxin, is one of snake venom toxin. It is composed of a phospholipase A2 subunit (A chain) and a smaller Kunitz-type protease inhibitor-like subunit (B chain), which are coupled covalently through a disulfide bond (see [9]).

Created Updated
2004-11-19 2009-02-26