DB code: D00144

RLCP classification 1.19.30000.10 : Hydrolysis
CATH domain 3.40.50.2300 : Rossmann fold
3.40.50.180 : Rossmann fold Catalytic domain
E.C. 3.1.1.61
CSA 1chd
M-CSA 1chd
MACiE

CATH domain Related DB codes (homologues)
3.40.50.2300 : Rossmann fold M00143

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P04042 Chemotaxis response regulator protein-glutamate methylesterase
EC 3.1.1.61
NP_460874.1 (Protein)
NC_003197.1 (DNA/RNA sequence)
PF01339 (CheB_methylest)
PF00072 (Response_reg)
[Graphical View]

KEGG enzyme name
protein-glutamate methylesterase
chemotaxis-specific methylesterase
methyl-accepting chemotaxis protein methyl-esterase
CheB methylesterase
methylesterase CheB
protein methyl-esterase
protein carboxyl methylesterase
PME
protein methylesterase
protein-L-glutamate-5-O-methyl-ester acylhydrolase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P04042 CHEB_SALTY Protein L-glutamate O(5)-methyl ester + H(2)O = protein L-glutamate + methanol. Cytoplasm.

KEGG Pathways
Map code Pathways E.C.

Compound table
Substrates Products Intermediates
KEGG-id C04142 C00001 C00614 C00132
E.C.
Compound Protein glutamate methyl ester H2O Protein glutamate Methanol
Type peptide/protein H2O carboxyl group,peptide/protein carbohydrate
ChEBI 15377
17790
PubChem 22247451
962
887
1a2oA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1a2oB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1a2oA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1a2oB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1chdA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
PDB;1a2o & Swiss-prot;P04042

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1a2oA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 56(phosphorylation)
1a2oB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 56(phosphorylation)
1a2oA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 164;HIS 190;ASP 286 THR 165;MET 283(oxyanion hole)
1a2oB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 164;HIS 190;ASP 286 THR 165;MET 283(oxyanion hole)
1chdA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 164;HIS 190;ASP 286 THR 165;MET 283(oxyanion hole)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[2]
Fig.9, p.279-282

References
[1]
Resource
Comments PHOSPHORYLATION.
Medline ID 88165060
PubMed ID 3280143
Journal Cell
Year 1988
Volume 53
Pages 79-87
Authors Hess JF, Oosawa K, Kaplan N, Simon MI
Title Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis.
Related PDB
Related UniProtKB P04042
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 152-349.
Medline ID 95333182
PubMed ID 7608974
Journal J Mol Biol
Year 1995
Volume 250
Pages 276-90
Authors West AH, Martinez-Hackert E, Stock AM
Title Crystal structure of the catalytic domain of the chemotaxis receptor methylesterase, CheB.
Related PDB 1chd
Related UniProtKB P04042
[3]
Resource
Comments
Medline ID
PubMed ID 7567955
Journal Proteins
Year 1995
Volume 21
Pages 345-50
Authors West AH, Djordjevic S, Martinez-Hackert E, Stock AM
Title Purification, crystallization, and preliminary X-ray diffraction analyses of the bacterial chemotaxis receptor modifying enzymes.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 9139915
Journal J Bacteriol
Year 1997
Volume 179
Pages 2963-8
Authors Brooun A, Zhang W, Alam M
Title Primary structure and functional analysis of the soluble transducer protein HtrXI in the archaeon Halobacterium salinarium.
Related PDB
Related UniProtKB
[5]
Resource
Comments CHARACTERIZATION.
Medline ID 98434376
PubMed ID 9760239
Journal Biochemistry
Year 1998
Volume 37
Pages 14038-47
Authors Anand GS, Goudreau PN, Stock AM
Title Activation of methylesterase CheB: evidence of a dual role for the regulatory domain.
Related PDB
Related UniProtKB P04042
[6]
Resource
Comments
Medline ID
PubMed ID 10049806
Journal J Struct Biol
Year 1998
Volume 124
Pages 189-200
Authors Djordjevic S, Stock AM
Title Structural analysis of bacterial chemotaxis proteins: components of a dynamic signaling system.
Related PDB
Related UniProtKB
[7]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
Medline ID 98132598
PubMed ID 9465023
Journal Proc Natl Acad Sci U S A
Year 1998
Volume 95
Pages 1381-6
Authors Djordjevic S, Goudreau PN, Xu Q, Stock AM, West AH
Title Structural basis for methylesterase CheB regulation by a phosphorylation-activated domain.
Related PDB 1a2o
Related UniProtKB P04042
[8]
Resource
Comments
Medline ID
PubMed ID 10231491
Journal Mol Microbiol
Year 1999
Volume 32
Pages 357-65
Authors Nishiyama SI, Umemura T, Nara T, Homma M, Kawagishi I
Title Conversion of a bacterial warm sensor to a cold sensor by methylation of a single residue in the presence of an attractant.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 10760170
Journal Mol Microbiol
Year 2000
Volume 36
Pages 132-40
Authors Shiomi D, Okumura H, Homma M, Kawagishi I
Title The aspartate chemoreceptor Tar is effectively methylated by binding to the methyltransferase mainly through hydrophobic interaction.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 11435446
Journal J Biol Chem
Year 2001
Volume 276
Pages 32984-9
Authors Barnakov AN, Barnakova LA, Hazelbauer GL
Title Location of the receptor-interaction site on CheB, the methylesterase response regulator of bacterial chemotaxis.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 11243808
Journal J Mol Biol
Year 2001
Volume 307
Pages 119-35
Authors Kim C, Jackson M, Lux R, Khan S
Title Determinants of chemotactic signal amplification in Escherichia coli.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 12022879
Journal Biochemistry
Year 2002
Volume 41
Pages 6752-60
Authors Anand GS, Stock AM
Title Kinetic basis for the stimulatory effect of phosphorylation on the methylesterase activity of CheB.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 12123464
Journal Mol Microbiol
Year 2002
Volume 45
Pages 555-68
Authors Zimmer MA, Szurmant H, Saulmon MM, Collins MA, Bant JS, Ordal GW
Title The role of heterologous receptors in McpB-mediated signalling in Bacillus subtilis chemotaxis.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-03-25 2009-02-26