DB code: D00153

RLCP classification 1.15.7910.1164 : Hydrolysis
CATH domain 3.30.540.10 : Fructose-1,6-Bisphosphatase; Chain A, domain 1 Catalytic domain
3.40.190.80 : D-Maltodextrin-Binding Protein; domain 2 Catalytic domain
E.C. 3.1.3.25
CSA 1ima
M-CSA 1ima
MACiE

CATH domain Related DB codes (homologues)
3.40.190.80 : D-Maltodextrin-Binding Protein; domain 2 D00148 D00491 T00053
3.30.540.10 : Fructose-1,6-Bisphosphatase; Chain A, domain 1 D00148 D00491 T00053

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
Q57573 Inositol-1-monophosphatase
I-1-Pase
IMPase
Inositol-1-phosphatase
EC 3.1.3.25
NP_247073.1 (Protein)
NC_000909.1 (DNA/RNA sequence)
PF00459 (Inositol_P)
[Graphical View]
O30298 Inositol-1-monophosphatase
I-1-Pase
IMPase
Inositol-1-phosphatase
EC 3.1.3.25
NP_071195.1 (Protein)
NC_000917.1 (DNA/RNA sequence)
PF00459 (Inositol_P)
[Graphical View]
P20456 Inositol monophosphatase 1
IMP 1
IMPase 1
EC 3.1.3.25
Inositol-1(or 4)-monophosphatase 1
Lithium-sensitive myo-inositol monophosphatase A1
NP_776786.1 (Protein)
NM_174361.4 (DNA/RNA sequence)
PF00459 (Inositol_P)
[Graphical View]
P29218 Inositol monophosphatase 1
IMP 1
IMPase 1
EC 3.1.3.25
Inositol-1(or 4)-monophosphatase 1
Lithium-sensitive myo-inositol monophosphatase A1
NP_001138350.1 (Protein)
NM_001144878.1 (DNA/RNA sequence)
NP_001138351.1 (Protein)
NM_001144879.1 (DNA/RNA sequence)
NP_005527.1 (Protein)
NM_005536.3 (DNA/RNA sequence)
PF00459 (Inositol_P)
[Graphical View]

KEGG enzyme name
inositol-phosphate phosphatase
myo-inositol-1(or 4)-monophosphatase
inositol 1-phosphatase
L-myo-inositol-1-phosphate phosphatase
myo-inositol 1-phosphatase
inositol phosphatase
inositol monophosphate phosphatase
inositol-1(or 4)-monophosphatase
myo-inositol-1(or 4)-phosphate phosphohydrolase
myo-inositol monophosphatase
myo-inositol-1-phosphatase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
Q57573 SUHB_METJA Myo-inositol phosphate + H(2)O = myo-inositol + phosphate. Homodimer. Magnesium.
O30298 SUHB_ARCFU Myo-inositol phosphate + H(2)O = myo-inositol + phosphate. Magnesium (By similarity).
P20456 IMPA1_BOVIN Myo-inositol phosphate + H(2)O = myo-inositol + phosphate. Homodimer. Cytoplasm. Magnesium.
P29218 IMPA1_HUMAN Myo-inositol phosphate + H(2)O = myo-inositol + phosphate. Homodimer. Cytoplasm. Magnesium.

KEGG Pathways
Map code Pathways E.C.
MAP00521 Streptomycin biosynthesis
MAP00562 Inositol phosphate metabolism
MAP04070 Phosphatidylinositol signaling system

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00305 C01177 C03546 C00001 C00137 C00009
E.C.
Compound Magnesium 1L-myo-Inositol 1-phosphate myo-Inositol 4-phosphate H2O myo-Inositol Orthophosphate
Type divalent metal (Ca2+, Mg2+) carbohydrate,phosphate group/phosphate ion carbohydrate,phosphate group/phosphate ion H2O carbohydrate phosphate group/phosphate ion
ChEBI 18420
18297
18384
15377
17268
26078
PubChem 888
22247451
962
1004
22486802
1dk4A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_ZN Unbound Unbound Unbound Bound:PO4
1dk4B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_ZN Unbound Unbound Unbound Bound:PO4
1g0hA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:2x_CA Bound:IPD Unbound Unbound Unbound
1g0hB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:2x_CA Bound:IPD Unbound Unbound Unbound
1g0iA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_MN Unbound Unbound Unbound Bound:PO4
1g0iB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_MN Unbound Unbound Unbound Bound:PO4
1lbvA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbvB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbwA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbwB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbxA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:2x_CA Bound:IPD Unbound Unbound Unbound
1lbxB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:2x_CA Bound:IPD Unbound Unbound Unbound
1lbyA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_MN Unbound Unbound Unbound Bound:PO4
1lbyB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_MN Unbound Unbound Unbound Bound:PO4
1lbzA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_CA Analogue:FBP Unbound Unbound Unbound
1lbzB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_CA Analogue:FBP Unbound Unbound Unbound
2bjiA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:3x_MG Unbound Unbound Unbound Unbound
2bjiB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:3x_MG Unbound Unbound Unbound Unbound
1awbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_CA Bound:IPD Unbound Unbound Unbound
1awbB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_CA Bound:IPD Unbound Unbound Unbound
1imaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_GD Bound:IPD Unbound Unbound Unbound
1imaB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_GD Bound:IPD Unbound Unbound Unbound
1imbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_GD Bound:LIP Unbound Unbound Unbound
1imbB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_GD Bound:LIP Unbound Unbound Unbound
1imcA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_MN Unbound Unbound Unbound Unbound
1imcB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:3x_MN Unbound Unbound Unbound Unbound
1imdA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:2x_MN Unbound Unbound Unbound Bound:PO4
1imdB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:2x_MN Unbound Unbound Unbound Bound:PO4
1imeA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_CA Unbound Unbound Unbound Unbound
1imeB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_CA Unbound Unbound Unbound Unbound
1imfA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
2hhmA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_GD Unbound Unbound Unbound Analogue:SO4
2hhmB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_GD Unbound Unbound Unbound Analogue:SO4
1dk4A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1dk4B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1g0hA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1g0hB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1g0iA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Bound:INS Unbound
1g0iB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Bound:INS Unbound
1lbvA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbvB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbwA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbwB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbxA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbxB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbyA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Analogue:F6P Unbound
1lbyB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Analogue:F6P Unbound
1lbzA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1lbzB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
2bjiA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
2bjiB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1awbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1awbB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imaB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imbB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imcA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imcB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imdA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imdB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imeA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imeB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1imfA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
2hhmA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
2hhmB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P29218, O30298, Q57573 & Literature [8], [20], [23], [27], [28]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1dk4A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 44;SER 86 GLU 65(Magnesium-1 & -3);ASP 81(Magnesium-1 & -2);ASP 84(Magnesium-2);ILE 83(Magnesium-1);ASP 38; (Magnesium-3) GLY 85;SER 86
1dk4B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 344;SER 386 GLU 365(Magnesium-1 & -3);ASP 381(Magnesium-1 & -2);ASP 384(Magnesium-2);ILE 383(Magnesium-1);ASP 338; (Magnesium-3) GLY 385;SER 386
1g0hA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 44;SER 86 GLU 65(Magnesium-1 & -3);ASP 81(Magnesium-1 & -2);ASP 84(Magnesium-2);ILE 83(Magnesium-1);ASP 38; (Magnesium-3) GLY 85;SER 86
1g0hB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 344;SER 386 GLU 365(Magnesium-1 & -3);ASP 381(Magnesium-1 & -2);ASP 384(Magnesium-2);ILE 383(Magnesium-1);ASP 338; (Magnesium-3) GLY 385;SER 386
1g0iA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 44;SER 86 GLU 65(Magnesium-1 & -3);ASP 81(Magnesium-1 & -2);ASP 84(Magnesium-2);ILE 83(Magnesium-1);ASP 38; (Magnesium-3) GLY 85;SER 86
1g0iB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 344;SER 386 GLU 365(Magnesium-1 & -3);ASP 381(Magnesium-1 & -2);ASP 384(Magnesium-2);ILE 383(Magnesium-1);ASP 338; (Magnesium-3) GLY 385;SER 386
1lbvA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 46;THR 87 GLU 67(Magnesium-1 & -3);ASP 82(Magnesium-1 & -2);ASP 85(Magnesium-2);LEU 84(Magnesium-1);ASP 38;THR 40(Magnesium-3) GLY 86;THR 87
1lbvB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 346;THR 387 GLU 367(Magnesium-1 & -3);ASP 382(Magnesium-1 & -2);ASP 385(Magnesium-2);LEU 384(Magnesium-1);ASP 338;THR 340(Magnesium-3) GLY 386;THR 387
1lbwA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 46;THR 87 GLU 67(Magnesium-1 & -3);ASP 82(Magnesium-1 & -2);ASP 85(Magnesium-2);LEU 84(Magnesium-1);ASP 38;THR 40(Magnesium-3) GLY 86;THR 87
1lbwB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 346;THR 387 GLU 367(Magnesium-1 & -3);ASP 382(Magnesium-1 & -2);ASP 385(Magnesium-2);LEU 384(Magnesium-1);ASP 338;THR 340(Magnesium-3) GLY 386;THR 387
1lbxA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 46;THR 87 GLU 67(Magnesium-1 & -3);ASP 82(Magnesium-1 & -2);ASP 85(Magnesium-2);LEU 84(Magnesium-1);ASP 38;THR 40(Magnesium-3) GLY 86;THR 87
1lbxB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 346;THR 387 GLU 367(Magnesium-1 & -3);ASP 382(Magnesium-1 & -2);ASP 385(Magnesium-2);LEU 384(Magnesium-1);ASP 338;THR 340(Magnesium-3) GLY 386;THR 387
1lbyA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 46;THR 87 GLU 67(Magnesium-1 & -3);ASP 82(Magnesium-1 & -2);ASP 85(Magnesium-2);LEU 84(Magnesium-1);ASP 38;THR 40(Magnesium-3) GLY 86;THR 87
1lbyB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 346;THR 387 GLU 367(Magnesium-1 & -3);ASP 382(Magnesium-1 & -2);ASP 385(Magnesium-2);LEU 384(Magnesium-1);ASP 338;THR 340(Magnesium-3) GLY 386;THR 387
1lbzA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 46;THR 87 GLU 67(Magnesium-1 & -3);ASP 82(Magnesium-1 & -2);ASP 85(Magnesium-2);LEU 84(Magnesium-1);ASP 38;THR 40(Magnesium-3) GLY 86;THR 87
1lbzB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 346;THR 387 GLU 367(Magnesium-1 & -3);ASP 382(Magnesium-1 & -2);ASP 385(Magnesium-2);LEU 384(Magnesium-1);ASP 338;THR 340(Magnesium-3) GLY 386;THR 387
2bjiA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 1047;THR 1095 GLU 1070(Magnesium-1 & -3);ASP 1090(Magnesium-1 & -2);ASP 1093(Magnesium-2);ILE 1092(Magnesium-1);ASP 1041; (Magnesium-3) GLY 1094;THR 1095
2bjiB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 2047;THR 2095 GLU 2070(Magnesium-1 & -3);ASP 2090(Magnesium-1 & -2);ASP 2093(Magnesium-2);ILE 2092(Magnesium-1);ASP 2041; (Magnesium-3) GLY 2094;THR 2095
1awbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1awbB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imaB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imbB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imcA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imcB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imdA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imdB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imeA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imeB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1imfA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
2hhmA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
2hhmB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 47;THR 95 GLU 70(Magnesium-1 & -3);ASP 90(Magnesium-1 & -2);ASP 93(Magnesium-2);ILE 92(Magnesium-1);ASP 41; (Magnesium-3) GLY 94;THR 95
1dk4A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 201(Magnesium-2)
1dk4B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 501(Magnesium-2)
1g0hA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 201(Magnesium-2)
1g0hB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 501(Magnesium-2)
1g0iA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 201(Magnesium-2)
1g0iB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 501(Magnesium-2)
1lbvA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 200(Magnesium-2)
1lbvB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 500(Magnesium-2)
1lbwA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 200(Magnesium-2)
1lbwB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 500(Magnesium-2)
1lbxA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 200(Magnesium-2)
1lbxB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 500(Magnesium-2)
1lbyA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 200(Magnesium-2)
1lbyB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 500(Magnesium-2)
1lbzA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 200(Magnesium-2)
1lbzB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 500(Magnesium-2)
2bjiA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 1220(Magnesium-2)
2bjiB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 2220(Magnesium-2)
1awbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1awbB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imaB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imbB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imcA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imcB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imdA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imdB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imeA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imeB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
1imfA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
2hhmA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)
2hhmB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 220(Magnesium-2)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[7]
p.9474-9475
[8]
Fig.5, p.9466
[10]
Fig.4, p.5-6
[16]
[20]
Fig.2, Fig.5, p.268-269
[27]
Fig.5, p.681-682
[28]
Fig.5, p.553

References
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Comments
Medline ID
PubMed ID 1665957
Journal Anal Biochem
Year 1991
Volume 198
Pages 375-8
Authors Churchich JE, Kwok F
Title Assay of 1L-myo-inositol-1-phosphatase using a fluorometric method.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 1333077
Journal Pharm Res
Year 1992
Volume 9
Pages 1370-3
Authors Harrold MW, Sriburi A, Caimano P, Qi H, Seybert D, Lin FT
Title Inhibition of myo-inositol 1-phosphatase by cis-1,2,3-cyclohexanetriol 1-phosphate, a molecular simplification of the natural substrate.
Related PDB
Related UniProtKB
[3]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS)
Medline ID 93066173
PubMed ID 1332026
Journal Proc Natl Acad Sci U S A
Year 1992
Volume 89
Pages 10031-5
Authors Bone R, Springer JP, Atack JR
Title Structure of inositol monophosphatase, the putative target of lithium therapy.
Related PDB 2hhm
Related UniProtKB P29218
[4]
Resource
Comments
Medline ID
PubMed ID 8382485
Journal Biochem Biophys Res Commun
Year 1993
Volume 190
Pages 1080-3
Authors Zhang Y, Liang JY, Lipscomb WN
Title Structural similarities between fructose-1,6-bisphosphatase and inositol monophosphatase.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 8223565
Journal Eur J Biochem
Year 1993
Volume 217
Pages 281-7
Authors Pollack SJ, Knowles MR, Atack JR, Broughton HB, Ragan CI, Osborne S, McAllister G
Title Probing the role of metal ions in the mechanism of inositol monophosphatase by site-directed mutagenesis.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 8019438
Journal Biochem Mol Biol Int
Year 1994
Volume 32
Pages 325-30
Authors Kwok F, Lo SC, Churchich JE
Title Brain myo-inositol monophosphatase: activity of the single subunit in a dimeric enzyme.
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Related UniProtKB
[7]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) IN COMPLEX WITH SUBSTRATE.
Medline ID
PubMed ID 8068621
Journal Biochemistry
Year 1994
Volume 33
Pages 9468-76
Authors Bone R, Frank L, Springer JP, Atack JR
Title Structural studies of metal binding by inositol monophosphatase: evidence for two-metal ion catalysis.
Related PDB 1imc 1imd 1ime 1imf
Related UniProtKB
[8]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8068620
Journal Biochemistry
Year 1994
Volume 33
Pages 9460-7
Authors Bone R, Frank L, Springer JP, Pollack SJ, Osborne SA, Atack JR, Knowles MR, McAllister G, Ragan CI, Broughton HB, et al
Title Structural analysis of inositol monophosphatase complexes with substrates.
Related PDB 1ima 1imb
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 8276805
Journal J Biol Chem
Year 1994
Volume 269
Pages 266-71
Authors Kwon OS, Churchich JE
Title Interaction of 70-kDA heat shock cognate protein with peptides and myo-inositol monophosphatase.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 7890024
Journal FEBS Lett
Year 1995
Volume 361
Pages 1-7
Authors Atack JR, Broughton HB, Pollack SJ
Title Structure and mechanism of inositol monophosphatase.
Related PDB
Related UniProtKB
[11]
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Comments
Medline ID
PubMed ID 7568043
Journal Proc Natl Acad Sci U S A
Year 1995
Volume 92
Pages 8916-20
Authors Villeret V, Huang S, Fromm HJ, Lipscomb WN
Title Crystallographic evidence for the action of potassium, thallium, and lithium ions on fructose-1,6-bisphosphatase.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 7761465
Journal Proc Natl Acad Sci U S A
Year 1995
Volume 92
Pages 5149-53
Authors York JD, Ponder JW, Majerus PW
Title Definition of a metal-dependent/Li(+)-inhibited phosphomonoesterase protein family based upon a conserved three-dimensional core structure.
Related PDB
Related UniProtKB
[13]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF MUTANT GLN-36, AND MUTAGENESIS OF LYS-36.
Medline ID
PubMed ID 8718889
Journal Biochemistry
Year 1996
Volume 35
Pages 10957-66
Authors Ganzhorn AJ, Lepage P, Pelton PD, Strasser F, Vincendon P, Rondeau JM
Title The contribution of lysine-36 to catalysis by human myo-inositol monophosphatase.
Related PDB
Related UniProtKB P29218
[14]
Resource
Comments
Medline ID
PubMed ID 8925839
Journal Eur J Biochem
Year 1996
Volume 240
Pages 288-91
Authors Saudek V, Vincendon P, Do QT, Atkinson RA, Sklenar V, Pelton PD, Piriou F, Ganzhorn AJ
Title 7Li nuclear-magnetic-resonance study of lithium binding to myo-inositolmonophosphatase.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 9435892
Journal J Med Chem
Year 1997
Volume 40
Pages 4208-21
Authors Piettre SR, Andre C, Chanal MC, Ducep JB, Lesur B, Piriou F, Raboisson P, Rondeau JM, Schelcher C, Zimmermann P, Ganzhorn AJ
Title Monoaryl- and bisaryldihydroxytropolones as potent inhibitors of inositol monophosphatase.
Related PDB
Related UniProtKB
[16]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)
Medline ID
PubMed ID
Journal PRotein Eng
Year 1997
Volume 10
Pages 61
Authors Ganzhorn AJ, Rondeau JM
Title Structure of an enzyme-substrate complex and the catalytic mechanism of human brain myo-inositol monophosphatase.
Related PDB 1awb
Related UniProtKB P29218
[17]
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Comments
Medline ID
PubMed ID 9647837
Journal Appl Environ Microbiol
Year 1998
Volume 64
Pages 2609-15
Authors Chen L, Roberts MF
Title Cloning and expression of the inositol monophosphatase gene from Methanococcus jannaschii and characterization of the enzyme.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 9853417
Journal Biol Pharm Bull
Year 1998
Volume 21
Pages 1218-21
Authors Tsuda J, Kimura T, Tanino H, Shimohama S, Fujimoto S
Title Characterization of high- and low-molecular weight zinc-dependent acid phosphatases in bovine liver.
Related PDB
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID 9988525
Journal J Protein Chem
Year 1998
Volume 17
Pages 789-97
Authors Lau CK, Lo SC, Li W, Churchich DR, Kwok F, Churchich JE
Title Partially folded conformations of inositol monophosphatase endowed with catalytic activity.
Related PDB
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 11828418
Journal Chembiochem
Year 2000
Volume 1
Pages 262-71
Authors Miller DJ, Beaton MW, Wilkie J, Gani D
Title The 6-OH group of D-inositol 1-phosphate serves as an H-bond donor in the catalytic hydrolysis of the phosphate ester by inositol monophosphatase.
Related PDB
Related UniProtKB
[21]
Resource
Comments
Medline ID
PubMed ID 10656801
Journal J Mol Biol
Year 2000
Volume 295
Pages 927-38
Authors Albert A, Yenush L, Gil-Mascarell MR, Rodriguez PL, Patel S, Martinez-Ripoll M, Blundell TL, Serrano R
Title X-ray structure of yeast Hal2p, a major target of lithium and sodium toxicity, and identification of framework interactions determining cation sensitivity.
Related PDB
Related UniProtKB
[22]
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Comments X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND METAL IONS.
Medline ID
PubMed ID 11062561
Journal Nat Struct Biol
Year 2000
Volume 7
Pages 1046-50
Authors Stec B, Yang H, Johnson KA, Chen L, Roberts MF
Title MJ0109 is an enzyme that is both an inositol monophosphatase and the 'missing' archaeal fructose-1,6-bisphosphatase.
Related PDB 1dk4
Related UniProtKB Q57573
[23]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
Medline ID
PubMed ID 11170378
Journal Biochemistry
Year 2001
Volume 40
Pages 618-30
Authors Johnson KA, Chen L, Yang H, Roberts MF, Stec B
Title Crystal structure and catalytic mechanism of the MJ0109 gene product: a bifunctional enzyme with inositol monophosphatase and fructose 1,6-bisphosphatase activities.
Related PDB 1g0h 1g0i
Related UniProtKB Q57573
[24]
Resource
Comments
Medline ID
PubMed ID 11914086
Journal Biochemistry
Year 2002
Volume 41
Pages 4392-8
Authors Nigou J, Dover LG, Besra GS
Title Purification and biochemical characterization of Mycobacterium tuberculosis SuhB, an inositol monophosphatase involved in inositol biosynthesis.
Related PDB
Related UniProtKB
[25]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND METAL IONS.
Medline ID
PubMed ID 11940584
Journal J Biol Chem
Year 2002
Volume 277
Pages 22863-74
Authors Stieglitz KA, Johnson KA, Yang H, Roberts MF, Seaton BA, Head JF, Stec B
Title Crystal structure of a dual activity IMPase/FBPase (AF2372) from Archaeoglobus fulgidus. The story of a mobile loop.
Related PDB 1lbv 1lbw 1lbx 1lby 1lbz
Related UniProtKB O30298
[26]
Resource
Comments
Medline ID
PubMed ID 11882005
Journal J Med Chem
Year 2002
Volume 45
Pages 1363-73
Authors Fauroux CM, Lee M, Cullis PM, Douglas KT, Gore MG, Freeman S
Title Stereochemistry at phosphorus of the reaction catalyzed by myo-inositol monophosphatase.
Related PDB
Related UniProtKB
[27]
Resource
Comments
Medline ID
PubMed ID 11812139
Journal J Mol Biol
Year 2002
Volume 315
Pages 677-85
Authors Patel S, Yenush L, Rodriguez PL, Serrano R, Blundell TL
Title Crystal structure of an enzyme displaying both inositol-polyphosphate-1-phosphatase and 3'-phosphoadenosine-5'-phosphate phosphatase activities: a novel target of lithium therapy.
Related PDB
Related UniProtKB
[28]
Resource
Comments
Medline ID
PubMed ID 15858264
Journal Acta Crystallogr D Biol Crystallogr
Year 2005
Volume 61
Pages 545-55
Authors Gill R, Mohammed F, Badyal R, Coates L, Erskine P, Thompson D, Cooper J, Gore M, Wood S
Title High-resolution structure of myo-inositol monophosphatase, the putative target of lithium therapy.
Related PDB 2bji
Related UniProtKB
[29]
Resource
Comments
Medline ID
PubMed ID 15715947
Journal J Biochem Mol Biol
Year 2005
Volume 38
Pages 58-64
Authors Kim DW, Hong JW, Eum WS, Choi HS, Choi SH, Kim SY, Lee BR, An JJ, Lee SH, Lee SR, Kwon OS, Kwon HY, Cho SW, Lee KS, Park J, Choi SY
Title Inactivation of brain myo-inositol monophosphate phosphatase by pyridoxal-5'-phosphate.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the inositol monophosphatase family. Although this enzyme binds only two magnesium ions according to Swissprot data, it might binds a third ion. The catalytic mechanism of this enzyme seems to be similar to that of a homologous enzyme, 3'(2'),5'-bisphosphate nucleotidase (E.C. 3.1.3.7; D00148 in EzCatDB).
According to the literature [7], [8], [10], [20] & [28], the reaction proceeds by SN2-like mechanism as follows:
(1) Asp47 (of 1awb) acts as the general base to activate a water through Thr95 (probably acting as a proton shuttle). In addition, Magnesium-1, which binds the water and 1-phosphate of substrate, also activates the water probably by lowering its pKa. (If the third magnesium ion is available, it may assists the role of the magnesium-1).
(2) The activated water makes a nucleophilic attack on the phosphate group, leading to a trigonal bipyramidal transition state. This transition state is stabilized by Magnesium-1 and -2 ions. (Here, magnesium-2 stabilizes the negative charge developed on the leaving group.) Moreover, the mainchain amide groups of Gly94/Thr95 may stabilize the transition state (see [7]).
(3) The target bond, phosphoester bond, is broken.
(4) Another water, which is bound to 6-hydroxyl group of product, myo-inositol, seems to act as a acid to protonate the leaving 1-oxygen, through 6-hydroxyl group.

Created Updated
2005-08-05 2009-02-26