DB code: D00170

CATH domain 1.10.530.10 : Lysozyme Catalytic domain
3.30.20.10 : Endochitinase; domain 2 Catalytic domain
E.C. 3.2.1.14
CSA 1cns
M-CSA 1cns
MACiE

CATH domain Related DB codes (homologues)
1.10.530.10 : Lysozyme S00520 S00509

Uniprot Enzyme Name
UniprotKB Protein name Synonyms CAZy Pfam
P23951 26 kDa endochitinase 2
EC 3.2.1.14
CHI-26
GH19 (Glycoside Hydrolase Family 19)
PF00182 (Glyco_hydro_19)
[Graphical View]
O81934
Chitinase
GH19 (Glycoside Hydrolase Family 19)
PF00182 (Glyco_hydro_19)
[Graphical View]

KEGG enzyme name
chitinase
chitodextrinase
1,4-beta-poly-N-acetylglucosaminidase
poly-beta-glucosaminidase
beta-1,4-poly-N-acetyl glucosamidinase
poly[1,4-(N-acetyl-beta-D-glucosaminide)] glycanohydrolase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P23951 CHI2_HORVU Random hydrolysis of N-acetyl-beta-D- glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
O81934 O81934_CANEN

KEGG Pathways
Map code Pathways E.C.
MAP00530 Aminosugars metabolism

Compound table
Substrates Products Intermediates
KEGG-id C00461 C00851 C00001 C03518 C00140
E.C.
Compound Chitin Chitodextrin H2O N-Acetyl-D-glucosaminide N-Acetyl-D-glucosamine
Type amide group,polysaccharide amide group,polysaccharide H2O amide group,carbohydrate amide group,carbohydrate
ChEBI 15377
506227
PubChem 22247451
962
439174
1baaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1cnsA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1cnsB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2baaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1dxjA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1baaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1cnsA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1cnsB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2baaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1dxjA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1baaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 67
1cnsA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 67
1cnsB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 67
2baaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 67
1dxjA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 68
1baaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 89
1cnsA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 89
1cnsB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 89
2baaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 89
1dxjA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 90

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[3]
p.410-411

References
[1]
Resource
Comments
Medline ID
PubMed ID 1593638
Journal J Mol Biol
Year 1992
Volume 225
Pages 565-7
Authors Hart PJ, Ready MP, Robertus JD
Title Crystallization of an endochitinase from Hordeum vulgar L. seeds.
Related PDB
Related UniProtKB
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS)
Medline ID 93132784
PubMed ID 8421299
Journal J Mol Biol
Year 1993
Volume 229
Pages 189-93
Authors Hart PJ, Monzingo AF, Ready MP, Ernst SR, Robertus JD
Title Crystal structure of an endochitinase from Hordeum vulgare L. seeds.
Related PDB
Related UniProtKB P23951
[3]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 7739049
Journal J Mol Biol
Year 1995
Volume 248
Pages 402-13
Authors Hart PJ, Pfluger HD, Monzingo AF, Hollis T, Robertus JD
Title The refined crystal structure of an endochitinase from Hordeum vulgare L. seeds at 1.8 A resolution.
Related PDB 1baa 2baa
Related UniProtKB
[4]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID
Journal Acta Crystallogr D Biol Crystallogr
Year 1996
Volume 52
Pages 289-98
Authors Song HK, Suh SW
Title Refined structure of the chitinase from barley seeds at 2.0 angstrom resolution.
Related PDB 1cns
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 8831791
Journal J Mol Biol
Year 1996
Volume 262
Pages 243-57
Authors Terwisscha van Scheltinga AC, Hennig M, Dijkstra BW
Title The 1.8 A resolution structure of hevamine, a plant chitinase/lysozyme, and analysis of the conserved sequence and structure motifs of glycosyl hydrolase family 18.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 9148754
Journal Biochem J
Year 1997
Volume 322
Pages 815-22
Authors Andersen MD, Jensen A, Robertus JD, Leah R, Skriver K
Title Heterologous expression and characterization of wild-type and mutant forms of a 26 kDa endochitinase from barley (Hordeum vulgare L.).
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 9790582
Journal Mol Gen Genet
Year 1998
Volume 259
Pages 511-5
Authors Ohme-Takagi M, Meins F Jr, Shinshi H
Title A tobacco gene encoding a novel basic class II chitinase: a putative ancestor of basic class I and acidic class II chitinase genes.
Related PDB
Related UniProtKB
[8]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID
Journal Acta Crystallogr D Biol Crystallogr
Year 2000
Volume 56
Pages 1096-9
Authors Hahn M, Hennig M, Schlesier B, Hohne W
Title Structure of jack bean chitinase.
Related PDB 1dxj
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 11414619
Journal Plant Mol Biol
Year 2001
Volume 45
Pages 609-18
Authors Suarez V, Staehelin C, Arango R, Holtorf H, Hofsteenge J, Meins F Jr
Title Substrate specificity and antifungal activity of recombinant tobacco class I chitinases.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-03-25 2009-09-29