DB code: D00173

CATH domain -.-.-.- :
2.120.10.10 : Neuraminidase Catalytic domain
E.C. 3.2.1.18
CSA 7nn9 1a4g
M-CSA 7nn9 1a4g
MACiE

CATH domain Related DB codes (homologues)
2.120.10.10 : Neuraminidase M00310 T00064 T00065 T00208

Uniprot Enzyme Name
UniprotKB Protein name Synonyms CAZy Pfam
P03472 Neuraminidase
EC 3.2.1.18
GH34 (Glycoside Hydrolase Family 34)
PF00064 (Neur)
[Graphical View]
P05803 Neuraminidase
EC 3.2.1.18
GH34 (Glycoside Hydrolase Family 34)
PF00064 (Neur)
[Graphical View]
P27907 Neuraminidase
EC 3.2.1.18
GH34 (Glycoside Hydrolase Family 34)
PF00064 (Neur)
[Graphical View]

KEGG enzyme name
exo-alpha-sialidase
neuraminidase
sialidase
alpha-neuraminidase
acetylneuraminidase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P03472 NRAM_I75A5 Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. Homotetramer (By similarity). Virion membrane (By similarity). Apical cell membrane, Single-pass type II membrane protein (By similarity). Note=Preferentially accumulates at the apical plasma membrane in infected polarized epithelial cells, which is the virus assembly site. Uses lipid rafts for cell surface transport and apical sorting. In the virion, forms a mushroom-shaped spike on the surface of the membrane (By similarity). Binds 1 calcium ion (By similarity).
P05803 NRAM_I84A1 Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. Homotetramer (By similarity). Virion membrane (By similarity). Apical cell membrane, Single-pass type II membrane protein (By similarity). Note=Preferentially accumulates at the apical plasma membrane in infected polarized epithelial cells, which is the virus assembly site. Uses lipid rafts for cell surface transport and apical sorting. In the virion, forms a mushroom-shaped spike on the surface of the membrane (By similarity). Binds 1 calcium ion.
P27907 NRAM_INBBE Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. Homotetramer. Virion membrane (By similarity). Apical cell membrane, Single-pass type II membrane protein (By similarity). Note=Preferentially accumulates at the apical plasma membrane in infected polarized epithelial cells, which is the virus assembly site. In the virion, forms a mushroom-shaped spike on the surface of the membrane (By similarity).

KEGG Pathways
Map code Pathways E.C.
MAP00511 N-Glycan degradation
MAP00600 Sphingolipid metabolism
MAP01032 Glycan structures - degradation

Compound table
Substrates Products Intermediates
KEGG-id C00001 C06128 C04730 C04884 C04927 C06139 C06140 C00270 C02686 C01290 C06135 C04911 C06140 C06141
E.C.
Compound H2O N-Acetylneuraminyl-galactosylceramide (N-Acetylneuraminyl)-D-galactosyl-D-glucosylceramide N-Acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide N-Acetylneuraminyl-D-galactosyl-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide Neu5Ac-alpha2->8Neu5Ac-alpha2->3Gal-beta1->3GalNAc-beta1->4(Neu5Ac-alpha2->8Neu5Ac-alpha2->3)Gal-beta1->4Glc-beta1->1'Cer Neu5Ac-alpha2->3Gal-beta1->3GalNAc-beta1->4(Neu5Ac-alpha2->8Neu5Ac-alpha2->3)Gal-beta1->4Glc-beta1->1'Cer N-Acetylneuraminate Galactosylceramide beta-D-Galactosyl-1,4-beta-D-glucosylceramide GalNAc-beta1->4Gal-beta1->4Glc-beta1->1'Cer D-Galactosyl-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide Neu5Ac-alpha2->3Gal-beta1->3GalNAc-beta1->4(Neu5Ac-alpha2->8Neu5Ac-alpha2->3)Gal-beta1->4Glc-beta1->1'Cer Gal-beta1->3GalNAc-beta1->4(Neu5Ac-alpha2->8Neu5Ac-alpha2->3)Gal-beta1->4Glc-beta1->1'Cer
Type H2O amide group,carbohydrate,carboxyl group,lipid amide group,carbohydrate,carboxyl group,lipid,polysaccharide amide group,carbohydrate,carboxyl group,lipid,polysaccharide amide group,carbohydrate,carboxyl group,lipid,polysaccharide amide group,carbohydrate,carboxyl group,lipid,polysaccharide amide group,carbohydrate,carboxyl group,lipid,polysaccharide amide group,carbohydrate,carboxyl group amide group,carbohydrate,lipid amide group,carbohydrate,lipid,polysaccharide amide group,carbohydrate,lipid,polysaccharide amide group,carbohydrate,carboxyl group,lipid,polysaccharide amide group,carbohydrate,carboxyl group,lipid amide group,carbohydrate,carboxyl group,lipid,polysaccharide
ChEBI 15377
17012
PubChem 22247451
962
439197
1a14N Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1f8bA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:DAN
1f8cA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:4AM
1f8dA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:9AM
1f8eA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:49A
1inyA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Analogue:EQP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1l7fA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Analogue:BCZ Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1l7gA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Analogue:BCZ Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1l7hA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Analogue:BCZ Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1mweA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:SIA_1 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ncaN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ncbN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nccN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nmcA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nmcN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nnaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nnbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:DAN
1nncA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Analogue:ZMR Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2qwaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2qwbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:SIA_800 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2qwcA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:DAN
2qwdA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:4AM
2qweA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Analogue:GNA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2qwfA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:G20
2qwgA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Analogue:G28 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2qwhA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:G39
2qwiA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:G20
2qwjA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Analogue:G28 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2qwkA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:G39
3nn9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
4nn9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
5nn9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
6nn9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
7nn9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ncdN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nmaN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nmbN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a4gA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:ZMR
1a4gB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:ZMR
1a4qA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:DPC
1a4qB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:DPC
1nsbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nsbB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nscA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:SIA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nscB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:SIA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nsdA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:DAN
1nsdB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:DAN

Reference for Active-site residues
resource references E.C.
Literature [9]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1a14N Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1f8bA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1f8cA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1f8dA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1f8eA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1inyA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 278;TYR 405
1l7fA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1l7gA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant E119G
1l7hA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant R292K
1mweA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1ncaN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1ncbN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant N329D
1nccN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant I368R
1nmcA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1nmcN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1nnaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 152;GLU 279;TYR 406
1nnbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 152;GLU 279;TYR 406
1nncA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
2qwaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant R292K
2qwbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant R292K
2qwcA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant R292K
2qwdA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant R292K
2qweA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant R292K
2qwfA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant R292K
2qwgA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant R292K
2qwhA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant R292K
2qwiA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
2qwjA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant R292K
2qwkA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
3nn9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant N329D
4nn9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant I368R
5nn9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant A369D
6nn9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406 mutant K432N
7nn9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1ncdN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1nmaN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1nmbN Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 151;GLU 277;TYR 406
1a4gA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 148;GLU 275;TYR 408
1a4gB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 148;GLU 275;TYR 408
1a4qA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 148;GLU 275;TYR 408
1a4qB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 148;GLU 275;TYR 408
1nsbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 148;GLU 275;TYR 408
1nsbB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 148;GLU 275;TYR 408
1nscA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 148;GLU 275;TYR 408
1nscB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 148;GLU 275;TYR 408
1nsdA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 148;GLU 275;TYR 408
1nsdB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 148;GLU 275;TYR 408

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[12]

References
[1]
Resource
Comments
Medline ID
PubMed ID 3811232
Journal Virology
Year 1987
Volume 156
Pages 181-4
Authors Laver WG, Webster RG, Colman PM
Title Crystals of antibodies complexed with influenza virus neuraminidase show isosteric binding of antibody to wild-type and variant antigens.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 2569208
Journal Philos Trans R Soc Lond B Biol Sci
Year 1989
Volume 323
Pages 511-8
Authors Colman PM, Tulip WR, Varghese JN, Tulloch PA, Baker AT, Laver WG, Air GM, Webster RG
Title Three-dimensional structures of influenza virus neuraminidase-antibody complexes.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 1700825
Journal J Virol
Year 1990
Volume 64
Pages 5797-803
Authors Air GM, Laver WG, Webster RG
Title Mechanism of antigenic variation in an individual epitope on influenza virus N9 neuraminidase.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 1866839
Journal Ultramicroscopy
Year 1991
Volume 35
Pages 131-43
Authors Bullough PA, Tulloch PA
Title Spot-scan imaging of microcrystals of an influenza neuraminidase-antibody fragment complex.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 1920429
Journal J Mol Biol
Year 1991
Volume 221
Pages 487-97
Authors Tulip WR, Varghese JN, Baker AT, van Donkelaar A, Laver WG, Webster RG, Colman PM
Title Refined atomic structures of N9 subtype influenza virus neuraminidase and escape mutants.
Related PDB 3nn9 4nn9 5nn9 6nn9
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 1984652
Journal Virology
Year 1991
Volume 180
Pages 266-72
Authors Burmeister WP, Daniels RS, Dayan S, Gagnon J, Cusack S, Ruigrok RW
Title Sequence and crystallization of influenza virus B/Beijing/1/87 neuraminidase.
Related PDB 1nsb
Related UniProtKB
[7]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 1381757
Journal J Mol Biol
Year 1992
Volume 227
Pages 122-48
Authors Tulip WR, Varghese JN, Laver WG, Webster RG, Colman PM
Title Refined crystal structure of the influenza virus N9 neuraminidase-NC41 Fab complex.
Related PDB 1nca
Related UniProtKB
[8]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 1522584
Journal J Mol Biol
Year 1992
Volume 227
Pages 149-59
Authors Tulip WR, Varghese JN, Webster RG, Laver WG, Colman PM
Title Crystal structures of two mutant neuraminidase-antibody complexes with amino acid substitutions in the interface.
Related PDB 1ncb 1ncc 1ncd
Related UniProtKB
[9]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 83-470.
Medline ID 93381688
PubMed ID 8371267
Journal J Mol Biol
Year 1993
Volume 232
Pages 1069-83
Authors Bossart-Whitaker P, Carson M, Babu YS, Smith CD, Laver WG, Air GM
Title Three-dimensional structure of influenza A N9 neuraminidase and its complex with the inhibitor 2-deoxy 2,3-dehydro-N-acetyl neuraminic acid.
Related PDB 1nna 1nnb
Related UniProtKB P03472
[10]
Resource
Comments
Medline ID
PubMed ID 8497484
Journal Proteins
Year 1993
Volume 16
Pages 57-63
Authors Malby RL, Caldwell JB, Gruen LC, Harley VR, Ivancic N, Kortt AA, Lilley GG, Power BE, Webster RG, Colman PM, et al
Title Recombinant antineuraminidase single chain antibody: expression, characterization, and crystallization in complex with antigen.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 7680132
Journal Proteins
Year 1993
Volume 15
Pages 121-32
Authors Nuss JM, Whitaker PB, Air GM
Title Identification of critical contact residues in the NC41 epitope of a subtype N9 influenza virus neuraminidase.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 8069621
Journal Structure
Year 1993
Volume 1
Pages 19-26
Authors Burmeister WP, Henrissat B, Bosso C, Cusack S, Ruigrok RW
Title Influenza B virus neuraminidase can synthesize its own inhibitor.
Related PDB 1nsc 1nsd
Related UniProtKB
[13]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 7517697
Journal Biochemistry
Year 1994
Volume 33
Pages 7986-97
Authors Tulip WR, Harley VR, Webster RG, Novotny J
Title N9 neuraminidase complexes with antibodies NC41 and NC10: empirical free energy calculations capture specificity trends observed with mutant binding data.
Related PDB 1nma
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 8168505
Journal Eur J Biochem
Year 1994
Volume 221
Pages 151-7
Authors Kortt AA, Malby RL, Caldwell JB, Gruen LC, Ivancic N, Lawrence MC, Howlett GJ, Webster RG, Hudson PJ, Colman PM
Title Recombinant anti-sialidase single-chain variable fragment antibody. Characterization, formation of dimer and higher-molecular-mass multimers and the solution of the crystal structure of the single-chain variable fragment/sialidase complex.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 7507170
Journal J Mol Biol
Year 1994
Volume 235
Pages 747-59
Authors Nuss JM, Air GM
Title Defining the requirements for an antibody epitope on influenza virus neuraminidase: how tolerant are protein epitopes?
Related PDB
Related UniProtKB
[16]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
Medline ID 95086861
PubMed ID 7994573
Journal Structure
Year 1994
Volume 2
Pages 733-46
Authors Malby RL, Tulip WR, Harley VR, McKimm-Breschkin JL, Laver WG, Webster RG, Colman PM
Title The structure of a complex between the NC10 antibody and influenza virus neuraminidase and comparison with the overlapping binding site of the NC41 antibody.
Related PDB 1nmb
Related UniProtKB P05803
[17]
Resource
Comments
Medline ID
PubMed ID 7576085
Journal J Protein Chem
Year 1995
Volume 14
Pages 167-78
Authors Kortt AA, Guthrie RE, Hinds MG, Power BE, Ivancic N, Caldwell JB, Gruen LC, Norton RS, Hudson PJ
Title Solution properties of Escherichia coli-expressed VH domain of anti-neuraminidase antibody NC41.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 7549872
Journal Protein Sci
Year 1995
Volume 4
Pages 1081-7
Authors Varghese JN, Epa VC, Colman PM
Title Three-dimensional structure of the complex of 4-guanidino-Neu5Ac2en and influenza virus neuraminidase.
Related PDB 1nnc 7nn9
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID 7844831
Journal J Mol Biol
Year 1995
Volume 245
Pages 623-34
Authors White CL, Janakiraman MN, Laver WG, Philippon C, Vasella A, Air GM, Luo M
Title A sialic acid-derived phosphonate analog inhibits different strains of influenza virus neuraminidase with different efficiencies.
Related PDB 1iny
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 8535779
Journal Structure
Year 1995
Volume 3
Pages 853-9
Authors Davies G, Henrissat B
Title Structures and mechanisms of glycosyl hydrolases.
Related PDB
Related UniProtKB
[21]
Resource
Comments
Medline ID
PubMed ID 8552677
Journal Proc Natl Acad Sci U S A
Year 1996
Volume 93
Pages 7-12
Authors Davies DR, Cohen GH
Title Interactions of protein antigens with antibodies.
Related PDB
Related UniProtKB
[22]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 83-470.
Medline ID 98004480
PubMed ID 9342319
Journal Proc Natl Acad Sci U S A
Year 1997
Volume 94
Pages 11808-12
Authors Varghese JN, Colman PM, van Donkelaar A, Blick TJ, Sahasrabudhe A, McKimm-Breschkin JL
Title Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases.
Related PDB 1mwe
Related UniProtKB P03472
[23]
Resource
Comments
Medline ID
PubMed ID 9194168
Journal Protein Eng
Year 1997
Volume 10
Pages 423-33
Authors Kortt AA, Lah M, Oddie GW, Gruen CL, Burns JE, Pearce LA, Atwell JL, McCoy AJ, Howlett GJ, Metzger DW, Webster RG, Hudson PJ
Title Single-chain Fv fragments of anti-neuraminidase antibody NC10 containing five- and ten-residue linkers form dimers and with zero-residue linker a trimer.
Related PDB
Related UniProtKB
[24]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9642070
Journal J Mol Biol
Year 1998
Volume 279
Pages 901-10
Authors Malby RL, McCoy AJ, Kortt AA, Hudson PJ, Colman PM
Title Three-dimensional structures of single-chain Fv-neuraminidase complexes.
Related PDB 1a14 1nmc
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID 9655825
Journal Structure
Year 1998
Volume 6
Pages 735-46
Authors Varghese JN, Smith PW, Sollis SL, Blick TJ, Sahasrabudhe A, McKimm-Breschkin JL, Colman PM
Title Drug design against a shifting target: a structural basis for resistance to inhibitors in a variant of influenza virus neuraminidase.
Related PDB 2qwa 2qwb 2qwc 2qwd 2qwe 2qwe 2qwf 2qwg 2qwh 2qwi 2qwj 2qwk
Related UniProtKB
[26]
Resource
Comments
Medline ID
PubMed ID 9526556
Journal J Med Chem
Year 1998
Volume 41
Pages 798-807
Authors Taylor NR, Cleasby A, Singh O, Skarzynski T, Wonacott AJ, Smith PW, Sollis SL, Howes PD, Cherry PC, Bethell R, Colman P, Varghese J
Title Dihydropyrancarboxamides related to zanamivir: a new series of inhibitors of influenza virus sialidases. 2. Crystallographic and molecular modeling study of complexes of 4-amino-4H-pyran-6-carboxamides and sialidase from influenza virus types A and B.
Related PDB 1a4g 1a4q
Related UniProtKB
[27]
Resource
Comments
Medline ID
PubMed ID 11274459
Journal Protein Sci
Year 2001
Volume 10
Pages 689-96
Authors Smith BJ, Colman PM, Von Itzstein M, Danylec B, Varghese JN
Title Analysis of inhibitor binding in influenza virus neuraminidase.
Related PDB 1f8b 1f8c 1f8d 1f8e
Related UniProtKB
[28]
Resource
Comments
Medline ID
PubMed ID 12014958
Journal J Med Chem
Year 2002
Volume 45
Pages 2207-12
Authors Smith BJ, McKimm-Breshkin JL, McDonald M, Fernley RT, Varghese JN, Colman PM
Title Structural studies of the resistance of influenza virus neuramindase to inhibitors.
Related PDB 1l7f 1l7g 1l7h
Related UniProtKB
[29]
Resource
Comments
Medline ID
PubMed ID 12054776
Journal J Mol Biol
Year 2002
Volume 318
Pages 161-77
Authors Sheinerman FB, Honig B
Title On the role of electrostatic interactions in the design of protein-protein interfaces.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the glycosidase family-34. This enzyme is a retaining glycosidase enzyme.
According to literature [9], mutations at conserved residues, Glu277 and Tyr406 (of 1a14), inactivated the enzyme, which suggested that they can be involved in catalysis.
Another paper [12] reported that Tyr406 might stabilize the transition state during catalysis. Acording to the paper, the catalytic mechanism of this enzyme is distinct from that of other glycosidase enzymes.

Created Updated
2004-05-29 2009-02-26