DB code: D00219

RLCP classification 1.13.30000.9 : Hydrolysis
CATH domain 3.30.70.80 : Alpha-Beta Plaits
3.40.50.200 : Rossmann fold Catalytic domain
E.C. 3.4.21.62
CSA 1sca
M-CSA 1sca
MACiE

CATH domain Related DB codes (homologues)
3.40.50.200 : Rossmann fold S00295 S00296 S00519

Uniprot Enzyme Name
UniprotKB Protein name Synonyms MEROPS Pfam RefSeq
P00780 Subtilisin Carlsberg
EC 3.4.21.62
I09.001 ()
PF05922 (Inhibitor_I9)
PF00082 (Peptidase_S8)
[Graphical View]
P00782 Subtilisin BPN''
EC 3.4.21.62
Alkaline protease
Subtilisin DFE
Subtilisin Novo
I09.001 ()
PF05922 (Inhibitor_I9)
PF00082 (Peptidase_S8)
[Graphical View]
P04189 Subtilisin E
EC 3.4.21.62
I09.001 ()
PF05922 (Inhibitor_I9)
PF00082 (Peptidase_S8)
[Graphical View]
NP_388911.2 (Protein)
NC_000964.3 (DNA/RNA sequence)
P07518 Subtilisin
EC 3.4.21.62
Alkaline mesentericopeptidase
S08.002 (Serine)
PF00082 (Peptidase_S8)
[Graphical View]

KEGG enzyme name
subtilisin
alcalase
alcalase 0.6L
alcalase 2.5L
ALK-enzyme
bacillopeptidase A
bacillopeptidase B
Bacillus subtilis alkaline proteinase bioprase
bioprase AL 15
bioprase APL 30
colistinase
(see also comments)
subtilisin J
subtilisin S41
subtilisin Sendai
subtilisin GX
subtilisin E
subtilisin BL
genenase I
esperase
maxatase
alcalase
thermoase PC 10
protease XXVII
thermoase
superase
subtilisin DY
subtilopeptidase
SP 266
savinase 8.0L
savinase 4.0T
kazusase
protease VIII
opticlean
Bacillus subtilis alkaline proteinase
protin A 3L
savinase
savinase 16.0L
savinase 32.0 L EX
orientase 10B
protease S

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P00780 SUBT_BACLI Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. Secreted. Binds 2 calcium ions per subunit.
P00782 SUBT_BACAM Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. Monomer. Secreted. Binds 2 calcium ions per subunit.
P04189 SUBT_BACSU Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. Secreted. Binds 2 calcium ions per subunit.
P07518 SUBT_BACPU Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. Secreted. Binds 2 calcium ions per subunit.

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00076 C00012 C00017 C00001 C00012 C00017 I00087 I00085 I00086
E.C.
Compound Calcium Peptide Protein H2O Peptide Protein Peptidyl-tetrahedral intermediate Acyl-enzyme Tetrahedral intermediate
Type divalent metal (Ca2+, Mg2+) peptide/protein peptide/protein H2O peptide/protein peptide/protein
ChEBI 29108
15377
PubChem 271
22247451
962
1scjB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1spbP Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1af4A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a2qA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ak9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1aqnA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1au9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1av7A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:2x_NA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1avtA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:2x_NA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1be6A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TCA Unbound
1be8A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TCA Unbound
1bfkA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1bfuA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1c3lA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Analogue:FMT_285 Unbound Unbound Unbound Unbound
1cseE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Bound:LEU_45-ASP_46 (chain I) Unbound Unbound Unbound Unbound Unbound
1duiA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_NA Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:DFP
1gnvA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1lw6E Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Bound:MET_59-GLU_60 (chain I) Unbound Unbound Unbound Unbound Unbound
1meeA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Bound:LYS_45-ASP_46 (chain I) Unbound Unbound Unbound Unbound Unbound
1oyvA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Bound:PHE_62-ASN_63 (chain I) Unbound Unbound Unbound Unbound Unbound
1oyvB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Bound:ARG_5-GLU_6 (chain I) Unbound Unbound Unbound Unbound Unbound
1s01A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1s02A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sbcA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sbhA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sbiA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sbnE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Bound:ARG_45-ASP_46 (chain I) Unbound Unbound Unbound Unbound Unbound
1sbtA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1scaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1scbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1scdA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1scnE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Intermediate-bound:BOC-ALA-PRO-APE (chain I) Unbound
1scjA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Bound:TYR_377 (chain B) Unbound Unbound Unbound
1selA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1selB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sibE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Bound:LEU_45-ASP_46 (chain I) Unbound Unbound Unbound Unbound Unbound
1spbS Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_NA Unbound Unbound Unbound Bound:TYR_77 (chain P) Unbound Unbound Unbound
1st2A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1suaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Bound:ALA-LEU-ALA-LEU (chain C) Unbound Unbound Unbound Unbound
1subA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sucA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:__K Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sudA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1sueA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_NA Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:DFP
1supA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_CA Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:PMS
1ubnA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1vsbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1yjaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1yjbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1yjcA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2sbtA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2secE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:3x_CA Unbound Bound:LEU_59-ASP_60 (chain I) Unbound Unbound Unbound Unbound Unbound
2sicE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Bound:MET_73-VAL_74 (chain I) Unbound Unbound Unbound Unbound Unbound
2sniE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Bound:MET_59-GLU_60 (chain I) Unbound Unbound Unbound Unbound Unbound
2st1A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
3sicE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Bound:LYS_73-VAL_74 (chain I) Unbound Unbound Unbound Unbound Unbound
3vsbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:2x_NA Unbound Unbound Unbound Unbound Unbound Unbound Unbound
5sicE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:2x_CA Unbound Bound:LYS_73-VAL_74 (chain I) Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P00780, P00782, P04189, P07518

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1scjB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1spbP Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1af4A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1a2qA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; MIS 221 MIS 221 mutant T22C, S87C, G169A, N218S
1ak9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant T22C, M50F, S87C, G169A, Y217K, N218S
1aqnA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant T22C, M50F, S87C, G169A, Q206C, Y217K, N218S
1au9A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant T22C, M50F, S87C, G169A, Q206C, Y217K, N218S
1av7A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; SBL 221 SBL 221
1avtA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; CLD 221 CLD 221
1be6A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1be8A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1bfkA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1bfuA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1c3lA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1cseE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1duiA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1gnvA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; MIS 221 MIS 221
1lw6E Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1meeA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1oyvA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1oyvB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1s01A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant M50F, N76D, G169A, Q206C, Y217K, N218S
1s02A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant Q19E, Q271E
1sbcA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1sbhA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant M50F, N76D, G169A, Q206C, N218S, K256Y
1sbiA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant M50F, N76D, G169A, Q206C, N218S
1sbnE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1sbtA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1scaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1scbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1scdA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1scnE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1scjA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; mutant S221C
1selA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; SEC 221 SEC 221
1selB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; SEC 221 SEC 221
1sibE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1spbS Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ;HIS 64;ASN 155;THR 220; mutant D32N, K43N, M50F, A73L, Q206V, Y217K, N218S, S221A, deletion 75-83
1st2A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1suaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; mutant K43N, M50F, G73L, Q206V, Y217K, N218S, S221A, Q271E, deletion 75-83
1subA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; mutant N218S, S221C
1sucA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; mutant M50P, Y217K, N218N, S221C, deletion 75-83
1sudA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; mutant M50P, Y217K, N218S, S221C
1sueA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant Q2K, S3C, P5S, K43N, M50F, A73L, Q206C, Y217K, N218S, deletion 75-83
1supA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
1ubnA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; SOC 221 SOC 221
1vsbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; CLB 221 CLB 221
1yjaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant M50F, N76D, G169A, Q206C, N218S, K256Y
1yjbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant M50F, N76D, G169A, Q206C, N218S, K256Y
1yjcA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221 mutant M50F, N76D, G169A, Q206C, N218S, K256Y
2sbtA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
2secE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
2sicE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
2sniE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
2st1A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
3sicE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221
3vsbA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220; SBD 221 SBD 221
5sicE Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 32;HIS 64;ASN 155;THR 220;SER 221 SER 221

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[2]
[5]
[8]
p.397-399
[9]
Fig.1
[10]
p.6595
[11]
p.7906
[12]
p.275
[16]
p.723
[17]
Fig.1
[18]
p.1219-1220
[19]
Fig.1, p.11800
[20]
Fig.2
[22]
p.314-316
[25]
Fig.4
[55]
Fig.5

References
[1]
Resource
Comments ACTIVE SITE.
Medline ID 69104413
PubMed ID 5249818
Journal Proc Natl Acad Sci U S A
Year 1968
Volume 61
Pages 1440-7
Authors Markland FS, Shaw E, Smith EL
Title Identification of histidine 64 in the active site of subtilisin.
Related PDB
Related UniProtKB P00782
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
Medline ID 72035041
PubMed ID 4399039
Journal Philos Trans R Soc Lond B Biol Sci
Year 1970
Volume 257
Pages 119-24
Authors Alden RA, Wright CS, Kraut J
Title A hydrogen-bond network at the active site of subtilisin BPN'.
Related PDB
Related UniProtKB P00782
[3]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 5160720
Journal Biochem Biophys Res Commun
Year 1971
Volume 45
Pages 337-44
Authors Alden RA, Birktoft JJ, Kraut J, Robertus JD, Wright CS
Title Atomic coordinates for subtilisin BPN' (or Novo).
Related PDB 1sbt
Related UniProtKB
[4]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 4508127
Journal Cold Spring Harb Symp Quant Biol
Year 1972
Volume 36
Pages 107-16
Authors Drenth J, Hol WG, Jansonius JN, Koekoek R
Title A comparison of the three-dimensional structures of subtilisin BPN' and subtilisin novo.
Related PDB 2sbt
Related UniProtKB
[5]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF COMPLEX WITH INHIBITOR.
Medline ID 85033707
PubMed ID 6387152
Journal J Mol Biol
Year 1984
Volume 178
Pages 389-414
Authors Hirono S, Akagawa H, Mitsui Y, Iitaka Y
Title Crystal structure at 2.6 A resolution of the complex of subtilisin BPN' with streptomyces subtilisin inhibitor.
Related PDB
Related UniProtKB P00782
[6]
Resource
Comments
Medline ID
PubMed ID 3519213
Journal EMBO J
Year 1986
Volume 5
Pages 813-8
Authors Bode W, Papamokos E, Musil D, Seemueller U, Fritz H
Title Refined 1.2 A crystal structure of the complex formed between subtilisin Carlsberg and the inhibitor eglin c. Molecular structure of eglin and its detailed interaction with subtilisin.
Related PDB
Related UniProtKB
[7]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 3301348
Journal Eur J Biochem
Year 1987
Volume 166
Pages 673-92
Authors Bode W, Papamokos E, Musil D
Title The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis. Structural analysis, subtilisin structure and interface geometry.
Related PDB 1cse
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 3299704
Journal Science
Year 1987
Volume 237
Pages 394-9
Authors Carter P, Wells JA
Title Engineering enzyme specificity by "substrate-assisted catalysis".
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 3282170
Journal Nature
Year 1988
Volume 332
Pages 564-8
Authors Carter P, Wells JA
Title Dissecting the catalytic triad of a serine protease.
Related PDB
Related UniProtKB
[10]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 3064813
Journal Biochemistry
Year 1988
Volume 27
Pages 6582-98
Authors McPhalen CA, James MN
Title Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-c-subtilisin Carlsberg and CI-2-subtilisin Novo.
Related PDB 2sec 2sni
Related UniProtKB
[11]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 3286644
Journal J Biol Chem
Year 1988
Volume 263
Pages 7895-906
Authors Bott R, Ultsch M, Kossiakoff A, Graycar T, Katz B, Power S
Title The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.
Related PDB 1st2 2st1
Related UniProtKB
[12]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 3150541
Journal Protein Eng
Year 1988
Volume 2
Pages 271-6
Authors Neidhart DJ, Petsko GA
Title The refined crystal structure of subtilisin Carlsberg at 2.5 A resolution.
Related PDB 1sbc
Related UniProtKB
[13]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF MUTANT.
Medline ID 90057412
PubMed ID 2684274
Journal Biochemistry
Year 1989
Volume 28
Pages 7205-13
Authors Pantoliano MW, Whitlow M, Wood JF, Dodd SW, Hardman KD, Rollence ML, Bryan PN
Title Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding.
Related PDB 1s01 1a2q 1ak9 1au9
Related UniProtKB P00782
[14]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 2127106
Journal Protein Eng
Year 1990
Volume 4
Pages 87-97
Authors Erwin CR, Barnett BL, Oliver JD, Sullivan JF
Title Effects of engineered salt bridges on the stability of subtilisin BPN'.
Related PDB 1s02
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 2199972
Journal Proteins
Year 1990
Volume 7
Pages 343-57
Authors Katz B, Kossiakoff AA
Title Crystal structures of subtilisin BPN' variants containing disulfide bonds and cavities: concerted structural rearrangements induced by mutagenesis.
Related PDB
Related UniProtKB
[16]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
Medline ID 92172311
PubMed ID 1793542
Journal Acta Crystallogr B
Year 1991
Volume 47
Pages 707-30
Authors Dauter Z, Betzel C, Genov N, Pipon N, Wilson KS
Title Complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-C.
Related PDB 1mee
Related UniProtKB P07518
[17]
Resource
Comments
Medline ID
PubMed ID 2059622
Journal Biochemistry
Year 1991
Volume 30
Pages 6142-8
Authors Carter P, Abrahmsen L, Wells JA
Title Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 1991100
Journal Biochemistry
Year 1991
Volume 30
Pages 1211-21
Authors Kossiakoff AA, Ultsch M, White S, Eigenbrot C
Title Neutron structure of subtilisin BPN': effects of chemical environment on hydrogen-bonding geometries and the pattern of hydrogen-deuterium exchange in secondary structure elements.
Related PDB
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID 1904870
Journal J Biol Chem
Year 1991
Volume 266
Pages 11797-800
Authors Braxton S, Wells JA
Title The importance of a distal hydrogen bonding group in stabilizing the transition state in subtilisin BPN'.
Related PDB
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 1904871
Journal J Biol Chem
Year 1991
Volume 266
Pages 11801-9
Authors Mizushima N, Spellmeyer D, Hirono S, Pearlman D, Kollman P
Title Free energy perturbation calculations on binding and catalysis after mutating threonine 220 in subtilisin.
Related PDB
Related UniProtKB
[21]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 1992167
Journal J Mol Biol
Year 1991
Volume 217
Pages 353-71
Authors Heinz DW, Priestle JP, Rahuel J, Wilson KS, Grutter MG
Title Refined crystal structures of subtilisin novo in complex with wild-type and two mutant eglins. Comparison with other serine proteinase inhibitor complexes.
Related PDB 1sbn 1sib
Related UniProtKB
[22]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 1920411
Journal J Mol Biol
Year 1991
Volume 221
Pages 309-25
Authors Takeuchi Y, Satow Y, Nakamura KT, Mitsui Y
Title Refined crystal structure of the complex of subtilisin BPN' and Streptomyces subtilisin inhibitor at 1.8 A resolution.
Related PDB 2sic
Related UniProtKB
[23]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 1891457
Journal Protein Eng
Year 1991
Volume 4
Pages 501-8
Authors Takeuchi Y, Noguchi S, Satow Y, Kojima S, Kumagai I, Miura K, Nakamura KT, Mitsui Y
Title Molecular recognition at the active site of subtilisin BPN': crystallographic studies using genetically engineered proteinaceous inhibitor SSI (Streptomyces subtilisin inhibitor).
Related PDB 3sic 5sic
Related UniProtKB
[24]
Resource
Comments
Medline ID
PubMed ID 1453465
Journal J Mol Biol
Year 1992
Volume 228
Pages 580-95
Authors Goddette DW, Paech C, Yang SS, Mielenz JR, Bystroff C, Wilke ME, Fletterick RJ
Title The crystal structure of the Bacillus lentus alkaline protease, subtilisin BL, at 1.4 A resolution.
Related PDB
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID 8268166
Journal Biochemistry
Year 1993
Volume 32
Pages 13909-16
Authors Jackson SE, Fersht AR
Title Contribution of long-range electrostatic interactions to the stabilization of the catalytic transition state of the serine protease subtilisin BPN'.
Related PDB
Related UniProtKB
[26]
Resource
Comments
Medline ID
PubMed ID 8347611
Journal Biochemistry
Year 1993
Volume 32
Pages 8112-9
Authors Strausberg S, Alexander P, Wang L, Schwarz F, Bryan P
Title Catalysis of a protein folding reaction: thermodynamic and kinetic analysis of subtilisin BPN' interactions with its propeptide fragment.
Related PDB
Related UniProtKB
[27]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF MUTANT WITH SELENOCYSTEINE-325.
Medline ID 93291170
PubMed ID 8512925
Journal Biochemistry
Year 1993
Volume 32
Pages 6157-64
Authors Syed R, Wu ZP, Hogle JM, Hilvert D
Title Crystal structure of selenosubtilisin at 2.0-A resolution.
Related PDB 1sel
Related UniProtKB P00780
[28]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8378343
Journal Proc Natl Acad Sci U S A
Year 1993
Volume 90
Pages 8653-7
Authors Fitzpatrick PA, Steinmetz AC, Ringe D, Klibanov AM
Title Enzyme crystal structure in a neat organic solvent.
Related PDB 1sca 1scb
Related UniProtKB
[29]
Resource
Comments
Medline ID
PubMed ID 8332597
Journal Protein Eng
Year 1993
Volume 6
Pages 397-408
Authors Heiner AP, Berendsen HJ, van Gunsteren WF
Title Structure prediction of subtilisin BPN' mutants using molecular dynamics methods.
Related PDB
Related UniProtKB
[30]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8332608
Journal Proteins
Year 1993
Volume 16
Pages 205-13
Authors Gallagher T, Bryan P, Gilliland GL
Title Calcium-independent subtilisin by design.
Related PDB 1sub 1suc 1sud
Related UniProtKB
[31]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8297378
Journal Biochem Biophys Res Commun
Year 1994
Volume 198
Pages 675-81
Authors Fitzpatrick PA, Ringe D, Klibanov AM
Title X-ray crystal structure of cross-linked subtilisin Carlsberg in water vs. acetonitrile.
Related PDB 1scd
Related UniProtKB
[32]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8068694
Journal Biochemistry
Year 1994
Volume 33
Pages 10535-44
Authors Steinmetz AC, Demuth HU, Ringe D
Title Inactivation of subtilisin Carlsberg by N-((tert-butoxycarbonyl)alanylprolylphenylalanyl)-O-benzoylhydroxyl- amine: formation of a covalent enzyme-inhibitor linkage in the form of a carbamate derivative.
Related PDB 1scn
Related UniProtKB
[33]
Resource
Comments
Medline ID
PubMed ID 7547973
Journal Biochemistry
Year 1995
Volume 34
Pages 12302-10
Authors Wangikar PP, Rich JO, Clark DS, Dordick JS
Title Probing enzymic transition state hydrophobicities.
Related PDB
Related UniProtKB
[34]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8535784
Journal Structure
Year 1995
Volume 3
Pages 907-14
Authors Gallagher T, Gilliland G, Wang L, Bryan P
Title The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'.
Related PDB 1spb
Related UniProtKB
[35]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)
Medline ID
PubMed ID
Journal Acta Crystallogr D Biol Crystallogr
Year 1996
Volume 52
Pages 1125-35
Authors Gallagher T, Oliver J, Bott R, Betzel C, Gilliland GL
Title Subtilisin BPN' at 1.6-A resolution: analysis for discrete disorder and comparison of crystal forms.
Related PDB
Related UniProtKB P00782
[36]
Resource
Comments
Medline ID
PubMed ID 8796321
Journal Adv Exp Med Biol
Year 1996
Volume 379
Pages 159-69
Authors Gilliland GL, Gallagher DT, Alexander P, Bryan P
Title Crystal structure analysis of subtilisin BPN' mutants engineered for studying thermal stability.
Related PDB
Related UniProtKB
[37]
Resource
Comments
Medline ID
PubMed ID 8796307
Journal Adv Exp Med Biol
Year 1996
Volume 379
Pages 21-7
Authors Nonaka T, Suzuki T, Tanaka N, Saito S, Senda T, Miura K, Mitsui Y
Title Structure and function of subtilisin BPN' as studied through crystallographic studies on a series of its complexes with genetically engineered proteinaceous inhibitor SSI.
Related PDB
Related UniProtKB
[38]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID
Journal J Am Chem Soc
Year 1996
Volume 118
Pages 1645-50
Authors Kidd RD, Yennawar HP, Sears P, Wong CH, Farber GK
Title A Weak Calcium Binding Site in Subtilisin BPN' Has a Dramatic Effect on Protein Stability.
Related PDB 1sbi
Related UniProtKB
[39]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9113975
Journal Proc Natl Acad Sci U S A
Year 1997
Volume 94
Pages 4250-5
Authors Schmitke JL, Stern LJ, Klibanov AM
Title The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrile.
Related PDB 1af4
Related UniProtKB
[40]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9675126
Journal Biochem Biophys Res Commun
Year 1998
Volume 248
Pages 273-7
Authors Schmitke JL, Stern LJ, Klibanov AM
Title Organic solvent binding to crystalline subtilisin1 in mostly aqueous media and in the neat solvents.
Related PDB 1bfk 1bfu
Related UniProtKB
[41]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 106-379.
Medline ID 98087517
PubMed ID 9425066
Journal Biochemistry
Year 1998
Volume 37
Pages 451-62
Authors Stoll VS, Eger BT, Hynes RC, Martichonok V, Jones JB, Pai EF
Title Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes.
Related PDB 1av7 1avt 1vsb
Related UniProtKB P00780
[42]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
Medline ID 99030466
PubMed ID 9811547
Journal J Mol Biol
Year 1998
Volume 284
Pages 137-44
Authors Jain SC, Shinde U, Li Y, Inouye M, Berman HM
Title The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution.
Related PDB 1scj
Related UniProtKB P04189
[43]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID
Journal Journal of Crystal Growth
Year 1998
Volume 193
Pages 665-73
Authors Gallagher DT, Pan QW, Gilliland GL
Title Mechanism of ionic strength dependence of crystal growth rates in a subtilisin variant.
Related PDB 1sue
Related UniProtKB
[44]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9789015
Journal Proc Natl Acad Sci U S A
Year 1998
Volume 95
Pages 12918-23
Authors Schmitke JL, Stern LJ, Klibanov AM
Title Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water.
Related PDB 1be6 1be8
Related UniProtKB
[45]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9552156
Journal Proteins
Year 1998
Volume 31
Pages 21-32
Authors Almog O, Gallagher T, Tordova M, Hoskins J, Bryan P, Gilliland GL
Title Crystal structure of calcium-independent subtilisin BPN' with restored thermal stability folded without the prodomain.
Related PDB 1sua
Related UniProtKB
[46]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9443341
Journal Proteins
Year 1998
Volume 30
Pages 61-73
Authors Prange T, Schiltz M, Pernot L, Colloc'h N, Longhi S, Bourguet W, Fourme R
Title Exploring hydrophobic sites in proteins with xenon or krypton.
Related PDB 1c3l
Related UniProtKB
[47]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10350485
Journal Biochemistry
Year 1999
Volume 38
Pages 6659-67
Authors Dinakarpandian D, Shenoy BC, Hilvert D, McRee DE, McTigue M, Carey PR
Title Electric fields in active sites: substrate switching from null to strong fields in thiol- and selenol-subtilisins.
Related PDB 1ubn
Related UniProtKB
[48]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10048334
Journal Protein Sci
Year 1999
Volume 8
Pages 410-7
Authors Kidd RD, Sears P, Huang DH, Witte K, Wong CH, Farber GK
Title Breaking the low barrier hydrogen bond in a serine protease.
Related PDB 1yja 1yjb 1yjc
Related UniProtKB
[49]
Resource
Comments
Medline ID
PubMed ID 11524007
Journal Biochemistry
Year 2001
Volume 40
Pages 10634-9
Authors Alexander PA, Ruan B, Bryan PN
Title Cation-dependent stability of subtilisin.
Related PDB
Related UniProtKB
[50]
Resource
Comments
Medline ID
PubMed ID 11524008
Journal Biochemistry
Year 2001
Volume 40
Pages 10640-4
Authors Alexander PA, Ruan B, Strausberg SL, Bryan PN
Title Stabilizing mutations and calcium-dependent stability of subtilisin.
Related PDB
Related UniProtKB
[51]
Resource
Comments
Medline ID
PubMed ID 11523982
Journal Biochemistry
Year 2001
Volume 40
Pages 10411-6
Authors Stratton JR, Pelton JG, Kirsch JF
Title A novel engineered subtilisin BPN' lacking a low-barrier hydrogen bond in the catalytic triad.
Related PDB
Related UniProtKB
[52]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12011071
Journal J Biol Chem
Year 2002
Volume 277
Pages 27553-8
Authors Almog O, Gallagher DT, Ladner JE, Strausberg S, Alexander P, Bryan P, Gilliland GL
Title Structural basis of thermostability. Analysis of stabilizing mutations in subtilisin BPN'.
Related PDB 1gnv
Related UniProtKB
[53]
Resource
Comments
Medline ID
PubMed ID 11925221
Journal J Org Chem
Year 2002
Volume 67
Pages 2144-51
Authors Ema T, Jittani M, Furuie K, Utaka M, Sakai T
Title 5-[4-(1-Hydroxyethyl)phenyl]-10,15,20-triphenylporphyrin as a probe of the transition-state conformation in hydrolase-catalyzed enantioselective transesterifications.
Related PDB
Related UniProtKB
[54]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12142461
Journal Proc Natl Acad Sci U S A
Year 2002
Volume 99
Pages 10316-21
Authors Radisky ES, Koshland DE Jr
Title A clogged gutter mechanism for protease inhibitors.
Related PDB 1lw6
Related UniProtKB
[55]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12684499
Journal J Biol Chem
Year 2003
Volume 278
Pages 24062-71
Authors Barrette-Ng IH, Ng KK, Cherney MM, Pearce G, Ryan CA, James MN
Title Structural basis of inhibition revealed by a 1:2 complex of the two-headed tomato inhibitor-II and subtilisin Carlsberg.
Related PDB 1oyv
Related UniProtKB
[56]
Resource
Comments
Medline ID
PubMed ID 12649438
Journal Protein Sci
Year 2003
Volume 12
Pages 794-810
Authors Day RM, Thalhauser CJ, Sudmeier JL, Vincent MP, Torchilin EV, Sanford DG, Bachovchin CW, Bachovchin WW
Title Tautomerism, acid-base equilibria, and H-bonding of the six histidines in subtilisin BPN' by NMR.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the peptidase family-S8.
The calcium ions are not involved in catalysis.
This enzyme has got a classic catalytic triad, composed of Ser/His/Asp, as in trypsin (D00197 in EzCatDB), suggesting that it has a similar mechanism to that of trypsin. However, in contrast to trypsin-like enzymes (where mainchain amide groups form an oxyanion hole), sidechains of Asn155 and Thr220 form an oxyanion hole, which stabilizes the transition-state, together with the mainchain amide group of Ser221 (see [11], [19] & [20]).

Created Updated
2004-11-12 2011-02-21