DB code: D00254

CATH domain 1.10.12.10 : Lyase 2-enoyl-coa Hydratase; Chain A, domain 2
3.90.226.10 : 2-enoyl-CoA Hydratase; Chain A, domain 1 Catalytic domain
E.C. 4.1.1.41
CSA 1ef8
M-CSA 1ef8
MACiE M0070

CATH domain Related DB codes (homologues)
3.90.226.10 : 2-enoyl-CoA Hydratase; Chain A, domain 1 M00145 M00122 S00849

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P52045 Methylmalonyl-CoA decarboxylase
MMCD
EC 4.1.1.41
Transcarboxylase
NP_417394.4 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491119.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF00378 (ECH)
[Graphical View]

KEGG enzyme name
methylmalonyl-CoA decarboxylase
propionyl-CoA carboxylase
propionyl coenzyme A carboxylase
methylmalonyl-coenzyme A decarboxylase
(S)-2-methyl-3-oxopropanoyl-CoA carboxy-lyase [incorrect]
(S)-methylmalonyl-CoA carboxy-lyase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P52045 MMCD_ECOLI (S)-methylmalonyl-CoA = propanoyl-CoA + CO(2). Dimer of homotrimers.

KEGG Pathways
Map code Pathways E.C.
MAP00640 Propanoate metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00120 C00683 C00100 C00011
E.C.
Compound Biotin (S)-2-Methyl-3-oxopropanoyl-CoA Propanoyl-CoA CO2
Type amide group,amine group,fatty acid,sulfide group amine group,carbohydrate,carboxyl group,nucleotide ,peptide/protein,sulfide group amine group,carbohydrate,nucleotide ,peptide/protein,sulfide group others
ChEBI 15956
15466
15539
16526
PubChem 171548
11966111
439291
439164
92753
280
1ef8A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ef8B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ef8C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ef9A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ef8A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ef8B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ef8C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ef9A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:2CP Unbound Unbound

Reference for Active-site residues
resource references E.C.
PDB;1ef8 & literature [12]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1ef8A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 220(Nickel binding)
1ef8B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 220(Nickel binding)
1ef8C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 220(Nickel binding)
1ef9A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ef8A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 140 HIS 66;GLY 110
1ef8B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 140 HIS 66;GLY 110
1ef8C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 140 HIS 66;GLY 110
1ef9A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 140 HIS 66;GLY 110

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[3]
Fig.1, Fig.6 2
[12]
Fig.6, p.4638-4639 2

References
[1]
Resource
Comments
Medline ID
PubMed ID 6852015
Journal Eur J Biochem
Year 1983
Volume 132
Pages 579-87
Authors Hilpert W, Dimroth P
Title Purification and characterization of a new sodium-transport decarboxylase. Methylmalonyl-CoA decarboxylase from Veillonella alcalescens.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 6705792
Journal Eur J Biochem
Year 1984
Volume 140
Pages 147-51
Authors Haase FC, Beegen H, Allen SH
Title Propionyl-coenzyme A carboxylase of Mycobacterium smegmatis. An electron microscopic study.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 3860170
Journal Ann N Y Acad Sci
Year 1985
Volume 447
Pages 140-51
Authors Fry DC, Fox T, Lane MD, Mildvan AS
Title NMR studies of the exchange of the amide protons of d-biotin and its derivatives.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 3464942
Journal Proc Natl Acad Sci U S A
Year 1986
Volume 83
Pages 8049-53
Authors Kraus JP, Firgaira F, Novotny J, Kalousek F, Williams KR, Williamson C, Ohura T, Rosenberg LE
Title Coding sequence of the precursor of the beta subunit of rat propionyl-CoA carboxylase.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 3609308
Journal FEBS Lett
Year 1987
Volume 220
Pages 121-5
Authors Hoffmann A, Dimroth P
Title Stereochemistry of the methylmalonyl-CoA decarboxylation reaction.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 1769208
Journal Comp Biochem Physiol B
Year 1991
Volume 99
Pages 613-7
Authors Garrastazu C, Iniesta MP, Aranguez MI, Ruiz Amil M
Title Comparative analysis of propionyl-CoA carboxylase from liver and mammary gland of mid-lactation cow.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 8227015
Journal J Biol Chem
Year 1993
Volume 268
Pages 24564-71
Authors Huder JB, Dimroth P
Title Sequence of the sodium ion pump methylmalonyl-CoA decarboxylase from Veillonella parvula.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 7601825
Journal J Bacteriol
Year 1995
Volume 177
Pages 3623-30
Authors Huder JB, Dimroth P
Title Expression of the sodium ion pump methylmalonyl-coenzyme A-decarboxylase from Veillonella parvula and of mutated enzyme specimens in Escherichia coli.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 8865276
Journal Pediatr Res
Year 1996
Volume 40
Pages 404-9
Authors Bergman AJ, Van der Knaap MS, Smeitink JA, Duran M, Dorland L, Valk J, Poll-The BT
Title Magnetic resonance imaging and spectroscopy of the brain in propionic acidemia: clinical and biochemical considerations.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 9683657
Journal Arch Microbiol
Year 1998
Volume 170
Pages 179-84
Authors Kimura Y, Kojyo T, Kimura I, Sato M
Title Propionyl-CoA carboxylase of Myxococcus xanthus: catalytic properties and function in developing cells.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 9887338
Journal Hum Mol Genet
Year 1999
Volume 8
Pages 107-13
Authors Campeau E, Dupuis L, Leclerc D, Gravel RA
Title Detection of a normally rare transcript in propionic acidemia patients with mRNA destabilizing mutations in the PCCA gene.
Related PDB
Related UniProtKB
[12]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10769118
Journal Biochemistry
Year 2000
Volume 39
Pages 4630-9
Authors Benning MM, Haller T, Gerlt JA, Holden HM
Title New reactions in the crotonase superfamily: structure of methylmalonyl CoA decarboxylase from Escherichia coli.
Related PDB 1ef8 1ef9
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 10769117
Journal Biochemistry
Year 2000
Volume 39
Pages 4622-9
Authors Haller T, Buckel T, Retey J, Gerlt JA
Title Discovering new enzymes and metabolic pathways: conversion of succinate to propionate by Escherichia coli.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 11136555
Journal Mol Genet Metab
Year 2000
Volume 71
Pages 623-32
Authors Chloupkova M, Ravn K, Schwartz M, Kraus JP
Title Changes in the carboxyl terminus of the beta subunit of human propionyl-CoA carboxylase affect the oligomer assembly and catalysis: expression and characterization of seven patient-derived mutant forms of PCC in Escherichia coli.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 11414279
Journal Mol Neurobiol
Year 2000
Volume 22
Pages 21-40
Authors Hassel B
Title Carboxylation and anaplerosis in neurons and glia.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-05-21 2009-02-26