DB code: D00274

RLCP classification 9.1050.440000.8010 : Hydride transfer
9.5010.536200.8010 : Hydride transfer
CATH domain 3.90.25.10 : UDP-galactose 4-epimerase; domain 1
3.40.50.720 : Rossmann fold Catalytic domain
E.C. 5.1.3.2
CSA
M-CSA
MACiE M0188

CATH domain Related DB codes (homologues)
3.40.50.720 : Rossmann fold S00543 S00551 S00552 S00553 S00602 S00604 S00605 S00608 S00610 S00625 S00319 S00328 S00329 S00330 S00331 S00332 D00456 D00457 D00458 S00324 S00320 S00325 S00326 S00327 D00459 S00335 S00336 S00334 T00219 S00339 D00513 D00001 D00002 D00003 D00005 D00007 D00008 D00010 D00012 D00017 D00018 D00023 D00027 D00028 D00031 D00032 D00033 D00034 D00035 D00037 D00048 D00071 D00476 D00481 D00482 D00490 D00492 D00494 D00545 D00601 D00603 D00604 D00605 D00615 D00845 D00857 D00858 M00161 M00171 M00210 T00002 T00010 T00011 T00015 T00227 T00247 T00408 T00414 D00827 D00262 D00275 M00035 T00109
3.90.25.10 : UDP-galactose 4-epimerase; domain 1 D00513 D00601 D00604 D00262 D00275

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P09147 UDP-glucose 4-epimerase
EC 5.1.3.2
Galactowaldenase
UDP-galactose 4-epimerase
NP_415280.3 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_489032.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF01370 (Epimerase)
PF13950 (Epimerase_Csub)
[Graphical View]
Q81K34
UDP-glucose 4-epimerase
EC 5.1.3.2
NP_847665.1 (Protein)
NC_003997.3 (DNA/RNA sequence)
YP_022172.1 (Protein)
NC_007530.2 (DNA/RNA sequence)
YP_031353.1 (Protein)
NC_005945.1 (DNA/RNA sequence)
PF01370 (Epimerase)
PF13950 (Epimerase_Csub)
[Graphical View]
Q3JPI3
UDP-glucose 4-epimerase
EC 5.1.3.2
YP_334520.1 (Protein)
NC_007434.1 (DNA/RNA sequence)
PF01370 (Epimerase)
PF13950 (Epimerase_Csub)
[Graphical View]
Q8T8E9
UDP-galactose 4-epimerase
EC 5.1.3.2
PF01370 (Epimerase)
PF13950 (Epimerase_Csub)
[Graphical View]
Q14376 UDP-glucose 4-epimerase
EC 5.1.3.2
Galactowaldenase
UDP-galactose 4-epimerase
NP_000394.2 (Protein)
NM_000403.3 (DNA/RNA sequence)
NP_001008217.1 (Protein)
NM_001008216.1 (DNA/RNA sequence)
NP_001121093.1 (Protein)
NM_001127621.1 (DNA/RNA sequence)
PF01370 (Epimerase)
PF13950 (Epimerase_Csub)
[Graphical View]

KEGG enzyme name
UDP-glucose 4-epimerase
UDP-galactose 4-epimerase
uridine diphosphoglucose epimerase
galactowaldenase
UDPG-4-epimerase
uridine diphosphate galactose 4-epimerase
uridine diphospho-galactose-4-epimerase
UDP-glucose epimerase
UDP-galactose 4-epimerase
4-epimerase
UDPG-4-epimerase
uridine diphosphoglucose 4-epimerase
uridine diphosphate glucose 4-epimerase
UDP-D-galactose 4-epimerase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P09147 GALE_ECOLI UDP-glucose = UDP-galactose. Homodimer. NAD.
Q81K34 Q81K34_BACAN
Q3JPI3 Q3JPI3_BURP1
Q8T8E9 Q8T8E9_9TRYP
Q14376 GALE_HUMAN UDP-glucose = UDP-galactose. Homodimer. NAD.

KEGG Pathways
Map code Pathways E.C.
MAP00052 Galactose metabolism
MAP00520 Nucleotide sugars metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00003 C00029 C00052 I00016
E.C.
Compound NAD+ UDP-glucose UDP-galactose UDP-4-ketohexopyranose
Type amide group,amine group,nucleotide amide group,carbohydrate,nucleotide amide group,carbohydrate,nucleotide
ChEBI 15846
46229
PubChem 5893
8629
23724458
1a9yA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
1a9zA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:UPG
1kvqA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
1kvrA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UDP Unbound
1kvsA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
1kvtA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
1kvuA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
1lrjA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UD1 Unbound
1lrkA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UD1 Unbound
1lrlA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
1nahA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UDP Unbound
1naiA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UDP Unbound
1udaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:UFG
1udbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UFG Unbound
1udcA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UFM Unbound
1xelA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
2udpA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UPP Unbound
2udpB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UPP Unbound
3enkA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:GUD Unbound
3enkB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:GUD Unbound
1gy8A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UDP Unbound
1gy8B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UDP Unbound
1gy8C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UDP Unbound
1gy8D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UDP Unbound
2cnbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:UFG
2cnbB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:UFG
2cnbC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:UFG
2cnbD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:UFG
1ek5A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1ek6A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
1ek6B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
1hzjA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UD1 Unbound
1hzjB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UD1 Unbound
1i3kA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
1i3kB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:UPG Unbound
1i3lA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:GDU
1i3lB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:GDU
1i3mA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UD1 Unbound
1i3mB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UD1 Unbound
1i3nA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UD1 Unbound
1i3nB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:UD2 Unbound
1a9yA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1a9zA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1kvqA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1kvrA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1kvsA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1kvtA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1kvuA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1lrjA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1lrkA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1lrlA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1nahA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1naiA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1udaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1udbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1udcA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1xelA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
2udpA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
2udpB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
3enkA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
3enkB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1gy8A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1gy8B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1gy8C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1gy8D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
2cnbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
2cnbB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
2cnbC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
2cnbD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1ek5A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1ek6A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:NAI Unbound Unbound
1ek6B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:NAI Unbound Unbound
1hzjA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1hzjB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1i3kA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1i3kB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1i3lA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1i3lB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1i3mA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1i3mB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1i3nA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound
1i3nB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound

Reference for Active-site residues
resource references E.C.
literature [22], [31], [35]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1a9yA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1a9zA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kvqA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kvrA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kvsA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kvtA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kvuA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1lrjA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1lrkA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant Y299C
1lrlA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant Y299C
1nahA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1naiA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1udaA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1udbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1udcA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1xelA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2udpA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2udpB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3enkA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3enkB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gy8A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gy8B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gy8C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gy8D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2cnbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2cnbB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2cnbC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2cnbD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ek5A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ek6A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ek6B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1hzjA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1hzjB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i3kA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i3kB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i3lA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i3lB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i3mA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i3mB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i3nA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1i3nB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1a9yA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ; ;LYS 153 mutant S124A, Y149F
1a9zA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ; ;LYS 153 mutant S124A, Y149F
1kvqA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ;TYR 149;LYS 153 mutant S124A
1kvrA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ;TYR 149;LYS 153 mutant S124A
1kvsA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ;TYR 149;LYS 153 mutant S124T
1kvtA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ;TYR 149;LYS 153 mutant S124V
1kvuA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;;LYS 153 mutant Y149F
1lrjA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
1lrkA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
1lrlA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
1nahA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
1naiA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
1udaA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
1udbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
1udcA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
1xelA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
2udpA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
2udpB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 124;TYR 149;LYS 153
3enkA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 128;TYR 152;LYS 156
3enkB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 128;TYR 152;LYS 156
1gy8A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 173;LYS 177
1gy8B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 173;LYS 177
1gy8C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 173;LYS 177
1gy8D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 173;LYS 177
2cnbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 173;LYS 177
2cnbB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 173;LYS 177
2cnbC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 173;LYS 177
2cnbD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 173;LYS 177
1ek5A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161
1ek6A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161
1ek6B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161
1hzjA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161
1hzjB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161
1i3kA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161 mutant V94M
1i3kB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161 mutant V94M
1i3lA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161 mutant V94M
1i3lB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161 mutant V94M
1i3mA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161 mutant V94M
1i3mB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161 mutant V94M
1i3nA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161 mutant V94M
1i3nB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 132;TYR 157;LYS 161 mutant V94M

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[3]
Fig.2, p.135-136
[6]
Fig.2, p.373
[7]
p.13227-13230
[8]
p.13235-13236
[11]
p.5139-5143
[12]
Fig.10, p.2156-2159
[13]
Scheme 1, Scheme 2, Scheme 3, p.10682-10683
[14]
p.10694-10695
[15]
Scheme 1, p.6294-6295, p.6302-6303
[18]
Scheme 1, p.11474-11477
[20]
Scheme 1, p.5697-5700
[22]
Scheme 1, Fig.4, p.6703-6705
[23]
Scheme 1, p.9187-9188
[24]
Scheme 1, Fig.6, p.11284-11286
[29]
p.250
[30]
Scheme 1
[31]
p.177-179
[32]
Fig.2, p.13371
[33]
Scheme 2, p.3065-3066
[35]
p.832-833

References
[1]
Resource
Comments
Medline ID
PubMed ID 3061645
Journal Carbohydr Res
Year 1988
Volume 179
Pages 289-99
Authors Kaca W, de Jongh-Leuvenink J, Zahringer U, Rietschel ET, Brade H, Verhoef J, Sinnwell V
Title Isolation and chemical analysis of 7-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-L-glycero-D-manno-heptose as a constituent of the lipopolysaccharides of the UDP-galactose epimerase-less mutant J-5 of Escherichia coli and Vibrio cholerae.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 2659075
Journal Biochemistry
Year 1989
Volume 28
Pages 2645-54
Authors Konopka JM, Halkides CJ, Vanhooke JL, Gorenstein DG, Frey PA
Title UDP-galactose 4-epimerase. Phosphorus-31 nuclear magnetic resonance analysis of NAD+ and NADH bound at the active site.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 2008433
Journal Proteins
Year 1991
Volume 9
Pages 135-42
Authors Bauer AJ, Rayment I, Frey PA, Holden HM
Title The isolation, purification, and preliminary crystallographic characterization of UDP-galactose-4-epimerase from Escherichia coli.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 1601848
Journal J Biol Chem
Year 1992
Volume 267
Pages 11714-20
Authors Bhattacharjee H, Bhaduri A
Title Distinct functional roles of two active site thiols in UDPglucose 4-epimerase from Kluyveromyces fragilis.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 1297789
Journal J Nutr Sci Vitaminol (Tokyo)
Year 1992
Volume Spec No
Pages 461-4
Authors Frey PA, Bauer AJ, Vanhooke JL, Konopka JM, Rayment I, Holden HM
Title Chemical spectroscopic and crystallographic studies of UDP-galactose 4-epimerase from Escherichia coli.
Related PDB
Related UniProtKB
[6]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
Medline ID 92253563
PubMed ID 1579570
Journal Proteins
Year 1992
Volume 12
Pages 372-81
Authors Bauer AJ, Rayment I, Frey PA, Holden HM
Title The molecular structure of UDP-galactose 4-epimerase from Escherichia coli determined at 2.5 A resolution.
Related PDB 1udp
Related UniProtKB P09147
[7]
Resource
Comments
Medline ID
PubMed ID 8241177
Journal Biochemistry
Year 1993
Volume 32
Pages 13220-30
Authors Burke JR, Frey PA
Title The importance of binding energy in catalysis of hydride transfer by UDP-galactose 4-epimerase: a 13C and 15N NMR and kinetic study.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 8241178
Journal Biochemistry
Year 1993
Volume 32
Pages 13231-6
Authors Swanson BA, Frey PA
Title Identification of lysine 153 as a functionally important residue in UDP-galactose 4-epimerase from Escherichia coli.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 8652544
Journal Biochemistry
Year 1996
Volume 35
Pages 7615-20
Authors Liu Y, Vanhooke JL, Frey PA
Title UDP-galactose 4-epimerase: NAD+ content and a charge-transfer band associated with the substrate-induced conformational transition.
Related PDB
Related UniProtKB
[10]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
Medline ID 96180680
PubMed ID 8611559
Journal Biochemistry
Year 1996
Volume 35
Pages 2557-66
Authors Thoden JB, Frey PA, Holden HM
Title Crystal structures of the oxidized and reduced forms of UDP-galactose 4-epimerase isolated from Escherichia coli.
Related PDB 1nah 1nai
Related UniProtKB P09147
[11]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8611497
Journal Biochemistry
Year 1996
Volume 35
Pages 5137-44
Authors Thoden JB, Frey PA, Holden HM
Title Molecular structure of the NADH/UDP-glucose abortive complex of UDP-galactose 4-epimerase from Escherichia coli: implications for the catalytic mechanism.
Related PDB 1xel
Related UniProtKB
[12]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
Medline ID 97084800
PubMed ID 8931134
Journal Protein Sci
Year 1996
Volume 5
Pages 2149-61
Authors Thoden JB, Frey PA, Holden HM
Title High-resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol.
Related PDB 2udp
Related UniProtKB P09147
[13]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9271498
Journal Biochemistry
Year 1997
Volume 36
Pages 10675-84
Authors Liu Y, Thoden JB, Kim J, Berger E, Gulick AM, Ruzicka FJ, Holden HM, Frey PA
Title Mechanistic roles of tyrosine 149 and serine 124 in UDP-galactose 4-epimerase from Escherichia coli.
Related PDB 1kvu
Related UniProtKB
[14]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF SER-124 MUTANTS.
Medline ID 97419132
PubMed ID 9271499
Journal Biochemistry
Year 1997
Volume 36
Pages 10685-95
Authors Thoden JB, Gulick AM, Holden HM
Title Molecular structures of the S124A, S124T, and S124V site-directed mutants of UDP-galactose 4-epimerase from Escherichia coli.
Related PDB 1kvq 1kvr 1kvs 1kvt
Related UniProtKB P09147
[15]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).
Medline ID 97317070
PubMed ID 9174344
Journal Biochemistry
Year 1997
Volume 36
Pages 6294-304
Authors Thoden JB, Hegeman AD, Wesenberg G, Chapeau MC, Frey PA, Holden HM
Title Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coli.
Related PDB 1uda 1udb 1udc
Related UniProtKB P09147
[16]
Resource
Comments
Medline ID
PubMed ID 9119006
Journal Eur J Biochem
Year 1997
Volume 244
Pages 407-13
Authors Dutta S, Maiti NR, Bhattacharyya D
Title Reversible folding of UDP-galactose 4-epimerase from Escherichia coli.
Related PDB
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID 9224707
Journal FEBS Lett
Year 1997
Volume 409
Pages 449-51
Authors Nayar S, Bhattacharyya D
Title UDP-galactose 4-epimerase from Escherichia coli: existence of a catalytic monomer.
Related PDB
Related UniProtKB
[18]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF MUTANTS.
Medline ID 98376428
PubMed ID 9708982
Journal Biochemistry
Year 1998
Volume 37
Pages 11469-77
Authors Thoden JB, Holden HM
Title Dramatic differences in the binding of UDP-galactose and UDP-glucose to UDP-galactose 4-epimerase from Escherichia coli.
Related PDB 1a9y 1a9z
Related UniProtKB P09147
[19]
Resource
Comments
Medline ID
PubMed ID 10329648
Journal J Biol Chem
Year 1999
Volume 274
Pages 14573-8
Authors Bhattacharyya U, Dhar G, Bhaduri A
Title An arginine residue is essential for stretching and binding of the substrate on UDP-glucose-4-epimerase from Escherichia coli. Use of a stacked and quenched uridine nucleotide fluorophore as probe.
Related PDB
Related UniProtKB
[20]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
Medline ID 20263487
PubMed ID 10801319
Journal Biochemistry
Year 2000
Volume 39
Pages 5691-701
Authors Thoden JB, Wohlers TM, Fridovich-Keil JL, Holden HM
Title Crystallographic evidence for Tyr 157 functioning as the active site base in human UDP-galactose 4-epimerase.
Related PDB 1ek5 1ek6
Related UniProtKB Q14376
[21]
Resource
Comments
Medline ID
PubMed ID 11437350
Journal Arch Biochem Biophys
Year 2001
Volume 391
Pages 188-96
Authors Barat B, Bhattacharyya D
Title UDP-galactose 4-epimerase from Escherichia coli: formation of catalytic site during reversible folding.
Related PDB
Related UniProtKB
[22]
Resource
Comments
Medline ID
PubMed ID 11380265
Journal Biochemistry
Year 2001
Volume 40
Pages 6699-705
Authors Berger E, Arabshahi A, Wei Y, Schilling JF, Frey PA
Title Acid-base catalysis by UDP-galactose 4-epimerase: correlations of kinetically measured acid dissociation constants with thermodynamic values for tyrosine 149.
Related PDB
Related UniProtKB
[23]
Resource
Comments
Medline ID
PubMed ID 11478886
Journal Biochemistry
Year 2001
Volume 40
Pages 9187-95
Authors Gerratana B, Cleland WW, Frey PA
Title Mechanistic roles of Thr134, Tyr160, and Lys 164 in the reaction catalyzed by dTDP-glucose 4,6-dehydratase.
Related PDB
Related UniProtKB
[24]
Resource
Comments
Medline ID
PubMed ID 11551228
Journal Biochemistry
Year 2001
Volume 40
Pages 11279-87
Authors Wei Y, Lin J, Frey PA
Title 13C NMR analysis of electrostatic interactions between NAD+ and active site residues of UDP-galactose 4-epimerase: implications for the activation induced by uridine nucleotides.
Related PDB
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID 11726498
Journal EMBO J
Year 2001
Volume 20
Pages 6619-26
Authors Stammers DK, Ren J, Leslie K, Nichols CE, Lamb HK, Cocklin S, Dodds A, Hawkins AR
Title The structure of the negative transcriptional regulator NmrA reveals a structural superfamily which includes the short-chain dehydrogenase/reductases.
Related PDB
Related UniProtKB
[26]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF VARIANT EDG MET-94.
Medline ID 21282994
PubMed ID 11279193
Journal J Biol Chem
Year 2001
Volume 276
Pages 20617-23
Authors Thoden JB, Wohlers TM, Fridovich-Keil JL, Holden HM
Title Molecular basis for severe epimerase deficiency galactosemia. X-ray structure of the human V94m-substituted UDP-galactose 4-epimerase.
Related PDB 1i3k 1i3l 1i3m 1i3n
Related UniProtKB Q14376
[27]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 11279032
Journal J Biol Chem
Year 2001
Volume 276
Pages 15131-6
Authors Thoden JB, Wohlers TM, Fridovich-Keil JL, Holden HM
Title Human UDP-galactose 4-epimerase. Accommodation of UDP-N-acetylglucosamine within the active site.
Related PDB 1hzj
Related UniProtKB
[28]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12019271
Journal J Biol Chem
Year 2002
Volume 277
Pages 27528-34
Authors Thoden JB, Henderson JM, Fridovich-Keil JL, Holden HM
Title Structural analysis of the Y299C mutant of Escherichia coli UDP-galactose 4-epimerase. Teaching an old dog new tricks.
Related PDB 1lrj 1lrk 1lrl
Related UniProtKB
[29]
Resource
Comments
Medline ID
PubMed ID 12604210
Journal Chem Biol Interact
Year 2003
Volume 143-144
Pages 247-53
Authors Oppermann U, Filling C, Hult M, Shafqat N, Wu X, Lindh M, Shafqat J, Nordling E, Kallberg Y, Persson B, Jornvall H
Title Short-chain dehydrogenases/reductases (SDR): the 2002 update.
Related PDB
Related UniProtKB
[30]
Resource
Comments
Medline ID
PubMed ID 12642575
Journal J Biol Chem
Year 2003
Volume 278
Pages 20874-81
Authors Koropatkin NM, Liu HW, Holden HM
Title High resolution x-ray structure of tyvelose epimerase from Salmonella typhi.
Related PDB
Related UniProtKB
[31]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12615316
Journal Mol Biochem Parasitol
Year 2003
Volume 126
Pages 173-80
Authors Shaw MP, Bond CS, Roper JR, Gourley DG, Ferguson MA, Hunter WN
Title High-resolution crystal structure of Trypanosoma brucei UDP-galactose 4'-epimerase: a potential target for structure-based development of novel trypanocides.
Related PDB 1gy8
Related UniProtKB
[32]
Resource
Comments
Medline ID
PubMed ID 15491143
Journal Biochemistry
Year 2004
Volume 43
Pages 13370-9
Authors Gatzeva-Topalova PZ, May AP, Sousa MC
Title Crystal structure of Escherichia coli ArnA (PmrI) decarboxylase domain. A key enzyme for lipid A modification with 4-amino-4-deoxy-L-arabinose and polymyxin resistance.
Related PDB
Related UniProtKB
[33]
Resource
Comments
Medline ID
PubMed ID 15023057
Journal Biochemistry
Year 2004
Volume 43
Pages 3057-67
Authors Vogan EM, Bellamacina C, He X, Liu HW, Ringe D, Petsko GA
Title Crystal structure at 1.8 A resolution of CDP-D-glucose 4,6-dehydratase from Yersinia pseudotuberculosis.
Related PDB
Related UniProtKB
[34]
Resource
Comments
Medline ID
PubMed ID 15175331
Journal J Biol Chem
Year 2004
Volume 279
Pages 32796-803
Authors Schulz JM, Watson AL, Sanders R, Ross KL, Thoden JB, Holden HM, Fridovich-Keil JL
Title Determinants of function and substrate specificity in human UDP-galactose 4'-epimerase.
Related PDB
Related UniProtKB
[35]
Resource
Comments
Medline ID
PubMed ID 16946458
Journal Acta Crystallograph Sect F Struct Biol Cryst Commun
Year 2006
Volume 62
Pages 829-34
Authors Alphey MS, Burton A, Urbaniak MD, Boons GJ, Ferguson MA, Hunter WN
Title Trypanosoma brucei UDP-galactose-4'-epimerase in ternary complex with NAD+ and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose.
Related PDB 2cnb
Related UniProtKB

Comments
This enzyme is a distant homologue of the short-chain dehydrogenase/reductase (SDR) superfamily, which includes Drosophia alcohol dehydrogenase (S00319 in EzCatDB). This enzyme has got a catalytic triad composed of conserved residues, Ser, Tyr, and Lys. The conformation of these residues, compared to that of the NAD molecule, seems to be similar to that of the homologous enzymes.
According to the literature [22], [31] and [35], this enzyme catalyzes the following reactions:
(A) Hydride transfer from C4' atom of substrate to NAD+, forming 4-keto intermediate and NADH.
This reaction mechanism must be the same as that by Drosophia alcohol dehydrogenase.
(X) Rotation or conformation change of the intermediate.
(B) Hydride transfer from NADH to C4' atom of the 4-ketose intermediate, recovering NAD+.
This reaction mechanism also must be the same as the reverse reaction by Drosophia alcohol dehydrogenase.

Created Updated
2005-06-07 2011-07-05