DB code: D00298

CATH domain 3.30.200.20 : Phosphorylase Kinase; domain 1
3.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1
E.C. 6.3.2.6
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.30.200.20 : Phosphorylase Kinase; domain 1 M00125 M00124 M00131 T00224 M00127 M00129 M00130 M00132 M00136 M00196 M00197 M00198 M00304 M00323 M00325 M00326 M00327 M00328 M00329 M00330 M00331 M00332 M00333 M00335 M00339 M00344
3.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1 T00082 M00035 M00037 M00051 T00107 T00108

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P27616 Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC 6.3.2.6
SAICAR synthetase
NP_009409.1 (Protein)
NM_001178216.1 (DNA/RNA sequence)
PF01259 (SAICAR_synt)
[Graphical View]

KEGG enzyme name
phosphoribosylaminoimidazolesuccinocarboxamide synthase
phosphoribosylaminoimidazole-succinocarboxamide synthetase
PurC
SAICAR synthetase
4-(N-succinocarboxamide)-5-aminoimidazole synthetase
4-[(N-succinylamino)carbonyl]-5-aminoimidazole ribonucleotidesynthetase
SAICARs
phosphoribosylaminoimidazolesuccinocarboxamide synthetase
5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P27616 PUR7_YEAST ATP + 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate. Monomer.

KEGG Pathways
Map code Pathways E.C.
MAP00230 Purine metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00305 C00002 C04751 C00049 C00008 C00009 C04823
E.C.
Compound Magnesium ATP 1-(5'-Phosphoribosyl)-5-amino-4-carboxyimidazole L-Aspartate ADP Orthophosphate 1-(5'-Phosphoribosyl)-5-amino-4-(N-succinocarboxamide)-imidazole
Type divalent metal (Ca2+, Mg2+) amine group,nucleotide amine group,carboxyl group,nucleotide amino acids,carboxyl group amine group,nucleotide phosphate group/phosphate ion amino acids,amide group,amine group,carbohydrate,nucleotide
ChEBI 18420
15422
28413
17053
16761
26078
18319
PubChem 888
5957
165388
44367445
5960
6022
1004
22486802
160666
1a48A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1a48A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P27616

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1a48A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1a48A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis

References
[1]
Resource
Comments
Medline ID
PubMed ID 1534690
Journal Biochemistry
Year 1992
Volume 31
Pages 5022-32
Authors Meyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM
Title Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 1447788
Journal J Mol Biol
Year 1992
Volume 228
Pages 298-9
Authors Grebenko AI, Levdikov VM, Barynin VV, Melik-Adamyan WR, Myasnikov AN
Title Crystallization and preliminary X-ray investigation of phosphoribosylaminoimidazolesuccinocarboxamide synthase from the yeast Saccharomyces cerevisiae.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 7918411
Journal Biochemistry
Year 1994
Volume 33
Pages 11927-34
Authors Firestine SM, Poon SW, Mueller EJ, Stubbe J, Davisson VJ
Title Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms.
Related PDB
Related UniProtKB
[4]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)
Medline ID 98212921
PubMed ID 9551557
Journal Structure
Year 1998
Volume 6
Pages 363-76
Authors Levdikov VM, Barynin VV, Grebenko AI, Melik-Adamyan WR, Lamzin VS, Wilson KS
Title The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis.
Related PDB 1a48
Related UniProtKB P27616

Comments

Created Updated
2004-03-25 2009-02-26