DB code: D00432

RLCP classification 1.13.30000.10 : Hydrolysis
CATH domain 2.40.10.10 : Thrombin, subunit H Catalytic domain
2.40.10.10 : Thrombin, subunit H Catalytic domain
E.C. 3.4.21.71
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
2.40.10.10 : Thrombin, subunit H M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00157 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq MEROPS Pfam
P08419 Elastase-2A
EC 3.4.21.71
Elastase-2
NP_999274.1 (Protein)
NM_214109.1 (DNA/RNA sequence)
S01.155 (Serine)
PF00089 (Trypsin)
[Graphical View]
Q29461 Elastase-2A
EC 3.4.21.71
Elastase-2
Elastase II
NP_777139.1 (Protein)
NM_174714.2 (DNA/RNA sequence)
S01.155 (Serine)
PF00089 (Trypsin)
[Graphical View]

KEGG enzyme name
pancreatic elastase II
pancreatic elastase 2

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
Q29461 ELA2A_BOVIN Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin. Secreted.
P08419 ELA2A_PIG Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin. Secreted.

KEGG Pathways
Map code Pathways E.C.

Compound table
Substrates Products Intermediates
KEGG-id C00373 C00001 C00012 I00087 I00085 I00086
E.C.
Compound Elastin H2O Peptide Peptidyl-tetrahedral intermediate Acyl-enzyme Tetrahedral intermediate
Type peptide/protein H2O peptide/protein
ChEBI 15377
PubChem 439221
962
22247451
1bruP01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Intermediate-analogue:1NB Unbound
1bruP02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P08419

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1bruP01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 195 GLY 193;SER 195
1bruP02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 57;ASP 102

References for Catalytic Mechanism
References Sections No. of steps in catalysis

References
[1]
Resource
Comments
Medline ID
PubMed ID 6094548
Journal J Biol Chem
Year 1984
Volume 259
Pages 14271-8
Authors Swift GH, Craik CS, Stary SJ, Quinto C, Lahaie RG, Rutter WJ, MacDonald RJ
Title Structure of the two related elastase genes expressed in the rat pancreas.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 3635654
Journal J Theor Biol
Year 1986
Volume 119
Pages 107-24
Authors Carlson GM, MacDonald RJ, Meyer EF Jr
Title Computer aided prediction and evaluation of the tertiary structure for rat elastase II.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 3634756
Journal Int J Pept Protein Res
Year 1986
Volume 27
Pages 183-90
Authors Vered M, Gertler A, Burstein Y
Title Partial amino acid sequence of porcine elastase II. Active site and the activation peptide regions.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 11838546
Journal J Protein Chem
Year 2001
Volume 20
Pages 577-84
Authors Azuma K, Banshou Y, Suzuki H
Title Bovine pancreatic elastase II cleaves Gln-Ile bond.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the peptidase family-S1.
This enzyme has got a catalytic triad, composed of Ser195/His57/Asp102, suggesting that it has a similar catalytic mechanism to that of trypsin.

Created Updated
2004-10-26 2011-02-21