DB code: D00458

CATH domain 3.40.192.10 : Leucine Dehydrogenase; Chain A, domain 1 Catalytic domain
3.40.50.720 : Rossmann fold
E.C. 1.1.1.38
CSA 1do8
M-CSA 1do8
MACiE M0021

CATH domain Related DB codes (homologues)
3.40.50.720 : Rossmann fold S00543 S00551 S00552 S00553 S00602 S00604 S00605 S00608 S00610 S00625 S00319 S00328 S00329 S00330 S00331 S00332 D00456 D00457 S00324 S00320 S00325 S00326 S00327 D00459 S00335 S00336 S00334 T00219 S00339 D00513 D00001 D00002 D00003 D00005 D00007 D00008 D00010 D00012 D00017 D00018 D00023 D00027 D00028 D00031 D00032 D00033 D00034 D00035 D00037 D00048 D00071 D00476 D00481 D00482 D00490 D00492 D00494 D00545 D00601 D00603 D00604 D00605 D00615 D00845 D00857 D00858 M00161 M00171 M00210 T00002 T00010 T00011 T00015 T00227 T00247 T00408 T00414 D00827 D00262 D00274 D00275 M00035 T00109
3.40.192.10 : Leucine Dehydrogenase; Chain A, domain 1 D00032 D00033 D00035 D00605 D00845 D00857 D00858 M00210 T00010 T00011 T00414

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam RefSeq
P27443 NAD-dependent malic enzyme, mitochondrial
NAD-ME
EC 1.1.1.38
PF00390 (malic)
PF03949 (Malic_M)
[Graphical View]
P23368 NAD-dependent malic enzyme, mitochondrial
NAD-ME
EC 1.1.1.38
Malic enzyme 2
PF00390 (malic)
PF03949 (Malic_M)
[Graphical View]
NP_001161807.1 (Protein)
NM_001168335.1 (DNA/RNA sequence)
NP_002387.1 (Protein)
NM_002396.4 (DNA/RNA sequence)

KEGG enzyme name
malate dehydrogenase (oxaloacetate-decarboxylating)
'malic' enzyme
pyruvic-malic carboxylase
NAD+-specific malic enzyme
NAD+-malic enzyme
NAD+-linked malic enzyme

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P23368 MAOM_HUMAN (S)-malate + NAD(+) = pyruvate + CO(2) + NADH. Homotetramer. Mitochondrion matrix. Divalent metal cations. Prefers magnesium or manganese.
P27443 MAOM_ASCSU (S)-malate + NAD(+) = pyruvate + CO(2) + NADH. Homotetramer (By similarity). Mitochondrion matrix. Divalent metal cations. Prefers magnesium or manganese.

KEGG Pathways
Map code Pathways E.C.
MAP00620 Pyruvate metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C02148 C00149 C00003 C00006 C00022 C00011 C00004 C00005 C00036 I00001
E.C.
Compound Divalent metal (S)-Malate NAD+ NADP Pyruvate CO2 NADH NADPH Oxaloacetate Enolpyruvate
Type divalent metal (Ca2+, Mg2+) carbohydrate,carboxyl group amide group,amine group,nucleotide amide group,amine group,nucleotide carbohydrate,carboxyl group others amide group,amine group,nucleotide amide group,amine group,nucleotide
ChEBI 30797
15846
18009
32816
16526
16908
16474
PubChem 222656
5893
5886
1060
280
439153
5884
1llqA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1llqB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1o0sA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1o0sB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1do8A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OXL
1do8B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OXL
1do8C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OXL
1do8D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OXL
1efkA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:MAK Unbound
1efkB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:MAK Unbound
1efkC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:MAK Unbound
1efkD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:MAK Unbound
1eflA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Analogue:TTN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1eflB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Analogue:TTN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1eflC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Analogue:TTN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1eflD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Analogue:TTN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz3A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OXL
1gz3B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OXL
1gz3C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OXL
1gz3D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:OXL
1gz4A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Analogue:TTN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz4B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Analogue:TTN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz4C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Analogue:TTN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz4D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Analogue:TTN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj2A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Bound:MLT Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj2B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Bound:MLT Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj2C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Bound:MLT Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj2D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Bound:MLT Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj3A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Bound:PYR Unbound Unbound Unbound Unbound Unbound
1pj3B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Bound:PYR Unbound Unbound Unbound Unbound Unbound
1pj3C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Bound:PYR Unbound Unbound Unbound Unbound Unbound
1pj3D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Unbound Unbound Unbound Bound:PYR Unbound Unbound Unbound Unbound Unbound
1pj4A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Bound:MLT Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj4B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Bound:MLT Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj4C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Bound:MLT Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj4D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Bound:MLT Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_LU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_LU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_LU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_LU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_LU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_LU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlG01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_LU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlH01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:_LU Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qr6A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qr6B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1llqA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1llqB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1o0sA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:NAI Unbound Unbound Unbound
1o0sB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:NAI Unbound Unbound Unbound
1do8A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1do8B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_1601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1do8C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_2601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1do8D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_3601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1efkA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1efkB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_1601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1efkC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_2601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1efkD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_3601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1eflA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1eflB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_1601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1eflC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_2601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1eflD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_3601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz3A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz3B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz3C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz3D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz4A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz4B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz4C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gz4D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj2A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:NAI Unbound Unbound Unbound
1pj2B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:NAI Unbound Unbound Unbound
1pj2C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:NAI Unbound Unbound Unbound
1pj2D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:NAI Unbound Unbound Unbound
1pj3A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj3B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_1601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj3C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_2601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj3D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_3601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj4A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj4B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj4C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pj4D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ATP Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_1601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_2601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_3601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_4601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_5601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlG02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_6601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pjlH02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_7601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qr6A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qr6B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:NAD_1601 Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
literature [13]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1llqA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 126;LYS 199;ASP 295 GLU 271;ASP 272;ASP 295(Divalent metal binding)
1llqB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 126;LYS 199;ASP 295 GLU 271;ASP 272;ASP 295(Divalent metal binding)
1o0sA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 126;LYS 199;ASP 295 GLU 271;ASP 272;ASP 295(Divalent metal binding)
1o0sB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 126;LYS 199;ASP 295 GLU 271;ASP 272;ASP 295(Divalent metal binding)
1do8A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1do8B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1do8C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1do8D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1efkA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1efkB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1efkC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1efkD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1eflA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1eflB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1eflC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1eflD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1gz3A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding) mutant R189Q
1gz3B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding) mutant R189Q
1gz3C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding) mutant R189Q
1gz3D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding) mutant R189Q
1gz4A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1gz4B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1gz4C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1gz4D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1pj2A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1pj2B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 1112;LYS 1183;ASP 1279 GLU 1255;ASP 1256;ASP 1279(Divalent metal binding)
1pj2C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 2112;LYS 2183;ASP 2279 GLU 2255;ASP 2256;ASP 2279(Divalent metal binding)
1pj2D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 3112;LYS 3183;ASP 3279 GLU 3255;ASP 3256;ASP 3279(Divalent metal binding)
1pj3A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1pj3B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 1112;LYS 1183;ASP 1279 GLU 1255;ASP 1256;ASP 1279(Divalent metal binding)
1pj3C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 2112;LYS 2183;ASP 2279 GLU 2255;ASP 2256;ASP 2279(Divalent metal binding)
1pj3D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 3112;LYS 3183;ASP 3279 GLU 3255;ASP 3256;ASP 3279(Divalent metal binding)
1pj4A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1pj4B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 1112;LYS 1183;ASP 1279 GLU 1255;ASP 1256;ASP 1279(Divalent metal binding)
1pj4C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 2112;LYS 2183;ASP 2279 GLU 2255;ASP 2256;ASP 2279(Divalent metal binding)
1pj4D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 3112;LYS 3183;ASP 3279 GLU 3255;ASP 3256;ASP 3279(Divalent metal binding)
1pjlA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1pjlB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 1112;LYS 1183;ASP 1279 GLU 1255;ASP 1256;ASP 1279(Divalent metal binding)
1pjlC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 2112;LYS 2183;ASP 2279 GLU 2255;ASP 2256;ASP 2279(Divalent metal binding)
1pjlD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 3112;LYS 3183;ASP 3279 GLU 3255;ASP 3256;ASP 3279(Divalent metal binding)
1pjlE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 4112;LYS 4183;ASP 4279 GLU 4255;ASP 4256;ASP 4279(Divalent metal binding)
1pjlF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 5112;LYS 5183;ASP 5279 GLU 5255;ASP 5256;ASP 5279(Divalent metal binding)
1pjlG01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 6112;LYS 6183;ASP 6279 GLU 6255;ASP 6256;ASP 6279(Divalent metal binding)
1pjlH01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 7112;LYS 7183;ASP 7279 GLU 7255;ASP 7256;ASP 7279(Divalent metal binding)
1qr6A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 112;LYS 183;ASP 279 GLU 255;ASP 256;ASP 279(Divalent metal binding)
1qr6B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 1112;LYS 1183;ASP 1279 GLU 1255;ASP 1256;ASP 1279(Divalent metal binding)
1llqA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1llqB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1o0sA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1o0sB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1do8A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1do8B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1do8C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1do8D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1efkA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1efkB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1efkC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1efkD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1eflA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1eflB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1eflC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1eflD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gz3A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant R523V, R556Q
1gz3B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant R523V, R556Q
1gz3C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant R523V, R556Q
1gz3D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant R523V, R556Q
1gz4A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gz4B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gz4C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gz4D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj2A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj2B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj2C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj2D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj3A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj3B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj3C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj3D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj4A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj4B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj4C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pj4D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pjlA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pjlB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pjlC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pjlD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pjlE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pjlF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pjlG02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pjlH02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1qr6A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1qr6B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[6]
Fig.1, p.10531-10532
[7]
p.884-885
[9]
Fig.3, p.254-255
[10]
Scheme 2, p.6935-6937
[12]
Scheme 1, p.38056-38058
[13]
Fig.5, p.1145-1149
[15]
Scheme 1

References
[1]
Resource
Comments
Medline ID
PubMed ID 2043616
Journal Biochemistry
Year 1991
Volume 30
Pages 5764-9
Authors Gavva SR, Harris BG, Weiss PM, Cook PF
Title Modification of a thiol at the active site of the Ascaris suum NAD-malic enzyme results in changes in the rate-determining steps for oxidative decarboxylation of L-malate.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 1993653
Journal J Biol Chem
Year 1991
Volume 266
Pages 2732-8
Authors Mallick S, Harris BG, Cook PF
Title Kinetic mechanism of NAD:malic enzyme from Ascaris suum in the direction of reductive carboxylation.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 8117666
Journal Biochemistry
Year 1994
Volume 33
Pages 2096-103
Authors Karsten WE, Cook PF
Title Stepwise versus concerted oxidative decarboxylation catalyzed by malic enzyme: a reinvestigation.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 7626027
Journal Biochem J
Year 1995
Volume 309
Pages 607-11
Authors Kochan Z, Karbowska J, Bukato G, Zydowo MM, Bertoli E, Tanfani F, Swierczynski J
Title A comparison of the secondary structure of human brain mitochondrial and cytosolic 'malic' enzyme investigated by Fourier-transform infrared spectroscopy.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 8844853
Journal Protein Sci
Year 1996
Volume 5
Pages 1648-54
Authors Tipton PA, Quinn TP, Peisach J, Cook PF
Title Role of the divalent metal ion in the NAD:malic enzyme reaction: an ESEEM determination of the ground state conformation of malate in the E:Mn:malate complex.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 10441149
Journal Biochemistry
Year 1999
Volume 38
Pages 10527-32
Authors Karsten WE, Chooback L, Liu D, Hwang CC, Lynch C, Cook PF
Title Mapping the active site topography of the NAD-malic enzyme via alanine-scanning site-directed mutagenesis.
Related PDB
Related UniProtKB
[7]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
Medline ID
PubMed ID 10467136
Journal Structure (Camb)
Year 1999
Volume 7
Pages 877-89
Authors Xu Y, Bhargava G, Wu H, Loeber G, Tong L
Title Crystal structure of human mitochondrial NAD(P)(+)-dependent malic enzyme: a new class of oxidative decarboxylases.
Related PDB 1qr6
Related UniProtKB P23368
[8]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10913357
Journal Biochem Biophys Res Commun
Year 2000
Volume 274
Pages 440-4
Authors Yang Z, Batra R, Floyd DL, Hung HC, Chang GG, Tong L
Title Potent and competitive inhibition of malic enzymes by lanthanide ions.
Related PDB 1pjl
Related UniProtKB
[9]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10700286
Journal Nat Struct Biol
Year 2000
Volume 7
Pages 251-7
Authors Yang Z, Floyd DL, Loeber G, Tong L
Title Structure of a closed form of human malic enzyme and implications for catalytic mechanism.
Related PDB 1do8 1efk 1efl
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 12033925
Journal Biochemistry
Year 2002
Volume 41
Pages 6928-38
Authors Coleman DE, Rao GS, Goldsmith EJ, Cook PF, Harris BG
Title Crystal structure of the malic enzyme from Ascaris suum complexed with nicotinamide adenine dinucleotide at 2.3 A resolution.
Related PDB 1llq
Related UniProtKB
[11]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12121650
Journal Structure (Camb)
Year 2002
Volume 10
Pages 951-60
Authors Yang Z, Lanks CW, Tong L
Title Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate.
Related PDB 1gz3 1gz4
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 12853453
Journal J Biol Chem
Year 2003
Volume 278
Pages 38051-8
Authors Rao GS, Coleman DE, Karsten WE, Cook PF, Harris BG
Title Crystallographic studies on Ascaris suum NAD-malic enzyme bound to reduced cofactor and identification of an effector site.
Related PDB 1o0s
Related UniProtKB
[13]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12962632
Journal Structure (Camb)
Year 2003
Volume 11
Pages 1141-50
Authors Tao X, Yang Z, Tong L
Title Crystal structures of substrate complexes of malic enzyme and insights into the catalytic mechanism.
Related PDB 1pj2 1pj3 1pj4
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 15182181
Journal Biochemistry
Year 2004
Volume 43
Pages 7382-90
Authors Hsu WC, Hung HC, Tong L, Chang GG
Title Dual functional roles of ATP in the human mitochondrial malic enzyme.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 14747989
Journal Proteins
Year 2004
Volume 54
Pages 404-11
Authors Kuo CW, Hung HC, Tong L, Chang GG
Title Metal-Induced reversible structural interconversion of human mitochondrial NAD(P)+-dependent malic enzyme.
Related PDB
Related UniProtKB

Comments
There are several types of malate deydrogenases.
1.1.1.37: Malate dehydrogenase / bacteria, rat, porcine
1.1.1.38: Malate dehydrogenase (oxaloacetate decarboxylating) / Human, Ascaris suum
1.1.1.39: Malate dehydrogenase (decarboxylating)
1.1.1.82: Malate dehydrogenase (NADP+) / Archaea, plant
1.1.1.40: Malate dehydrogenase (oxaloacetate decarboxylating) (NADP+) / pigeon
1.1.1.83: D-malate dehydrogenase (decarboxylating)
This enzyme catalyzes the following reactions:
(A) Hydride transfer from substrate, (S)-Malate, to NAD+, giving oxaloacetate and NADH:
(B) Eliminative double-bond formation; Elimination of CO2 from oxaloacetate, giving enolpyruvate:
(C) Isomerization (shift of double-bond position; from C=C-O to C-C=O):

Created Updated
2004-12-07 2009-02-26