DB code: D00475

CATH domain 3.40.605.10 : Aldehyde Dehydrogenase; Chain A, domain 1 Catalytic domain
3.40.309.10 : Aldehyde Dehydrogenase; Chain A, domain 2 Catalytic domain
E.C. 1.2.1.4
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.40.605.10 : Aldehyde Dehydrogenase; Chain A, domain 1 D00020 D00021 D00022 D00614
3.40.309.10 : Aldehyde Dehydrogenase; Chain A, domain 2 D00020 D00021 D00022 D00614

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
Q56694 NADP-dependent fatty aldehyde dehydrogenase
EC 1.2.1.4
PF00171 (Aldedh)
[Graphical View]

KEGG enzyme name
aldehyde dehydrogenase (NADP+)
NADP+-acetaldehyde dehydrogenase
NADP+-dependent aldehyde dehydrogenase
aldehyde dehydrogenase (NADP+)

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
Q56694 ALDH_VIBHA An aldehyde + NADP(+) + H(2)O = an acid + NADPH. Homodimer.

KEGG Pathways
Map code Pathways E.C.
MAP00930 Caprolactam degradation

Compound table
Substrates Products Intermediates
KEGG-id C00071 C00006 C00001 C00174 C00005 C00080
E.C.
Compound Aldehyde NADP+ H2O Acid NADPH H+
Type carbohydrate amide group,amine group,nucleotide H2O others amide group,amine group,nucleotide others
ChEBI 18009
15377
16474
15378
PubChem 5886
22247451
962
5884
1038
1cbzA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:NAP Unbound Unbound
1cbzB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:NAP Unbound Unbound
1cbzC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:NAP Unbound Unbound
1cbzD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:NAP Unbound Unbound
1co3A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound
1co3B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound
1co3C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound
1co3D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound
1eyyA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:NAP Unbound Unbound
1eyyB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:NAP Unbound Unbound
1eyyC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:NAP Unbound Unbound
1eyyD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:NAP Unbound Unbound
1ez0A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound
1ez0B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound
1ez0C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound
1ez0D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound
1cbzA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1cbzB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1cbzC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1cbzD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1co3A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1co3B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1co3C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1co3D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1eyyA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1eyyB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1eyyC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1eyyD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ez0A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ez0B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ez0C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ez0D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;Q56694 & literature [7]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1cbzA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1cbzB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1cbzC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1cbzD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1co3A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1co3B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1co3C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1co3D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1eyyA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1eyyB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1eyyC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1eyyD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1ez0A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1ez0B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1ez0C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1ez0D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASN 147;GLU 253;HIS 450
1cbzA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1cbzB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1cbzC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1cbzD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1co3A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1co3B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1co3C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1co3D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1eyyA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1eyyB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1eyyC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1eyyD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1ez0A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1ez0B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1ez0C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289
1ez0D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain CYS 289

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[5]
p.859-860
[6]
p.14411-14413, p.14414-14418
[7]
p.34-36

References
[1]
Resource
Comments
Medline ID
PubMed ID 8527447
Journal Biochemistry
Year 1995
Volume 34
Pages 16725-32
Authors Vedadi M, Szittner R, Smillie L, Meighen E
Title Involvement of cysteine 289 in the catalytic activity of an NADP(+)-specific fatty aldehyde dehydrogenase from Vibrio harveyi.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 8897616
Journal Protein Sci
Year 1996
Volume 5
Pages 2130-2
Authors Croteau N, Vedadi M, Delarge M, Meighen E, Abu-Abed M, Howell PL, Vrielink A
Title Crystallization and preliminary X-ray analysis of aldehyde dehydrogenase from Vibrio harveyi.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 9059630
Journal Adv Exp Med Biol
Year 1997
Volume 414
Pages 269-75
Authors Vedadi M, Croteau N, Delarge M, Vrielink A, Meighen E
Title Structural and functional studies of a NADP(+)-specific aldehyde dehydrogenase from the luminescent marine bacterium Vibrio harveyi.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 10471295
Journal Biochemistry
Year 1999
Volume 38
Pages 11440-7
Authors Zhang L, Ahvazi B, Szittner R, Vrielink A, Meighen E
Title Change of nucleotide specificity and enhancement of catalytic efficiency in single point mutants of Vibrio harveyi aldehyde dehydrogenase.
Related PDB
Related UniProtKB
[5]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS)
Medline ID 20363527
PubMed ID 10903148
Journal Biochem J
Year 2000
Volume 349 Pt 3
Pages 853-61
Authors Ahvazi B, Coulombe R, Delarge M, Vedadi M, Zhang L, Meighen E, Vrielink A
Title Crystal structure of the NADP+-dependent aldehyde dehydrogenase from Vibrio harveyi: structural implications for cofactor specificity and affinity.
Related PDB 1ez0 1eyy 1co3 1cbz
Related UniProtKB Q56694
[6]
Resource
Comments
Medline ID
PubMed ID 11087393
Journal Biochemistry
Year 2000
Volume 39
Pages 14409-18
Authors Zhang L, Ahvazi B, Szittner R, Vrielink A, Meighen E
Title A histidine residue in the catalytic mechanism distinguishes Vibrio harveyi aldehyde dehydrogenase from other members of the aldehyde dehydrogenase superfamily.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 11306028
Journal Chem Biol Interact
Year 2001
Volume 130-132
Pages 29-38
Authors Zhang L, Ahvazi B, Szittner R, Vrielink A, Meighen E
Title Differences in nucleotide specificity and catalytic mechanism between Vibrio harveyi aldehyde dehydrogenase and other members of the aldehyde dehydrogenase superfamily.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to aldehyde dehydrogenase superfamily.

Created Updated
2005-01-19 2009-02-26