DB code: D00541

CATH domain -.-.-.- :
3.10.270.10 : Urate Oxidase Catalytic domain
E.C. 1.7.3.3
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.10.270.10 : Urate Oxidase S00187

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Includes Pfam
Q45697 Uric acid degradation bifunctional protein
None 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
(OHCU decarboxylase)
EC 4.1.1.n1
Uricase
EC 1.7.3.3
Urate oxidase
PF09349 (OHCU_decarbox)
PF01014 (Uricase)
[Graphical View]

KEGG enzyme name
urate oxidase
uric acid oxidase
uricase
uricase II

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
Q45697 URIC_BACSB Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2).

KEGG Pathways
Map code Pathways E.C.
MAP00232 Caffeine metabolism
MAP00230 Purine metabolism

Compound table
Substrates Products Intermediates
KEGG-id C00366 C00007 C00001 C11821 C00027
E.C.
Compound Urate O2 H2O 5-Hydroxyisourate H2O2
Type amide group,aromatic ring (with nitrogen atoms) others H2O amide group,aromatic ring (with nitrogen atoms),imine group others
ChEBI 17775
46811
46814
46817
46823
62589
15379
26689
27140
15377
18072
16240
PubChem 1175
977
22247451
962
250388
22326046
784
1j2gA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:AZA Unbound Unbound Unbound
1j2gB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:AZA Unbound Unbound Unbound
1j2gC Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:AZA Unbound Unbound Unbound
1j2gD Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:AZA Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;Q00511 & literature [11], [18], [19]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1j2gA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 13;THR 73;ARG 201;GLN 250;ASN 276 THR 73
1j2gB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 13;THR 73;ARG 201;GLN 250;ASN 276 THR 73
1j2gC Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 13;THR 73;ARG 201;GLN 250;ASN 276 THR 73
1j2gD Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 13;THR 73;ARG 201;GLN 250;ASN 276 THR 73

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[8]
Fig.4, p.950-951
[9]
Scheme 1
[18]
Scheme 1, Fig.5, p.4097-4100
[19]
p.460-462

References
[1]
Resource
Comments
Medline ID
PubMed ID 5640165
Journal Biochim Biophys Acta
Year 1968
Volume 151
Pages 63-9
Authors Nose K, Arima K
Title Studies on bacterial urate:oxygen oxidoreductase. II. Observations concerning the properties and components of the active site.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 4696756
Journal Biochemistry
Year 1973
Volume 12
Pages 1358-63
Authors Pitts OM, Priest DG
Title Uricase reaction intermediate. Mechanism of borate and hydroxide ion catalysis.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 4834240
Journal Nature
Year 1974
Volume 249
Pages 490-1
Authors Naparstek A, Romette JL, Kernevez JP, Thomas D
Title Memory in enzyme membranes.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 455938
Journal Comput Biol Med
Year 1979
Volume 9
Pages 145-53
Authors Lam CF, Cross AP
Title Comparative study of parameter estimation procedures in enzymic kinetics.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 7194181
Journal Enzyme
Year 1981
Volume 26
Pages 49-53
Authors Nishimura H, Matsushima A, Inada Y
Title Improved modification of yeast uricase with polyethylene glycol, accompanied with nonimmunoreactivity towards anti-uricase serum and high enzymic activity.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 9222322
Journal FEBS Lett
Year 1981
Volume 134
Pages 50-2
Authors Yoshida K, Nishimura H, Takahashi K, Matsushima A, Inada Y
Title Modification of amino groups in Candida utilis uricase with naphthoquinone disulfonic acid in relation to the enzymic activity.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 9125493
Journal Biochemistry
Year 1997
Volume 36
Pages 4731-8
Authors Kahn K, Tipton PA
Title Kinetic mechanism and cofactor content of soybean root nodule urate oxidase.
Related PDB
Related UniProtKB
[8]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)
Medline ID 98025064
PubMed ID 9360612
Journal Nat Struct Biol
Year 1997
Volume 4
Pages 947-52
Authors Colloc'h N, el Hajji M, Bachet B, L'Hermite G, Schiltz M, Prange T, Castro B, Mornon JP
Title Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 A resolution.
Related PDB 1uox
Related UniProtKB Q00511
[9]
Resource
Comments
Medline ID
PubMed ID 9709003
Journal Biochemistry
Year 1998
Volume 37
Pages 11651-9
Authors Kahn K, Tipton PA
Title Spectroscopic characterization of intermediates in the urate oxidase reaction.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 10361492
Journal Anal Chem
Year 1999
Volume 71
Pages 1928-34
Authors Nakaminami T, Ito S, Kuwabata S, Yoneyama H
Title Uricase-catalyzed oxidation of uric acid using an artificial electron acceptor and fabrication of amperometric uric acid sensors with use of a redox ladder polymer.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 10227848
Journal J Membr Biol
Year 1999
Volume 169
Pages 13-27
Authors Leal-Pinto E, Cohen BE, Abramson RG
Title Functional analysis and molecular modeling of a cloned urate transporter/channel.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 10737935
Journal Proteins
Year 2000
Volume 39
Pages 142-54
Authors Colloc'h N, Poupon A, Mornon JP
Title Sequence and structural features of the T-fold, an original tunnelling building unit.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 11856833
Journal Acta Crystallogr D Biol Crystallogr
Year 2002
Volume 58
Pages 472-9
Authors Vivares D, Bonnete F
Title X-ray scattering studies of Aspergillus flavus urate oxidase: towards a better understanding of PEG effects on the crystallization of large proteins.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 12060597
Journal Am J Physiol Renal Physiol
Year 2002
Volume 283
Pages F150-63
Authors Leal-Pinto E, Cohen BE, Lipkowitz MS, Abramson RG
Title Functional analysis and molecular model of the human urate transporter/channel, hUAT.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 12149119
Journal Biotechnol Appl Biochem
Year 2002
Volume 36
Pages 21-31
Authors Bayol A, Capdevielle J, Malazzi P, Buzy A, Claude Bonnet M, Colloc'h N, Mornon JP, Loyaux D, Ferrara P
Title Modification of a reactive cysteine explains differences between rasburicase and Uricozyme, a natural Aspergillus flavus uricase.
Related PDB
Related UniProtKB
[16]
Resource
Comments
Medline ID
PubMed ID 12208494
Journal FEBS Lett
Year 2002
Volume 526
Pages 5-10
Authors Colloc'h N, Mornon JP, Camadro JM
Title Towards a new T-fold protein?: the coproporphyrinogen III oxidase sequence matches many structural features from urate oxidase.
Related PDB
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID 12499547
Journal Acta Crystallogr D Biol Crystallogr
Year 2003
Volume 59
Pages 118-26
Authors Girard E, Stelter M, Anelli PL, Vicat J, Kahn R
Title A new class of gadolinium complexes employed to obtain high-phasing-power heavy-atom derivatives: results from SAD experiments with hen egg-white lysozyme and urate oxidase from Aspergillus flavus.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 12680763
Journal Biochemistry
Year 2003
Volume 42
Pages 4094-100
Authors Imhoff RD, Power NP, Borrok MJ, Tipton PA
Title General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diad.
Related PDB
Related UniProtKB
[19]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 14993669
Journal Acta Crystallogr D Biol Crystallogr
Year 2004
Volume 60
Pages 453-62
Authors Retailleau P, Colloc'h N, Vivares D, Bonnete F, Castro B, El-Hajji M, Mornon JP, Monard G, Prange T
Title Complexed and ligand-free high-resolution structures of urate oxidase (Uox) from Aspergillus flavus: a reassignment of the active-site binding mode.
Related PDB 1r4s 1r4u 1r51 1r56
Related UniProtKB
[20]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 15735331
Journal Acta Crystallogr D Biol Crystallogr
Year 2005
Volume 61
Pages 218-29
Authors Retailleau P, Colloc'h N, Vivares D, Bonnete F, Castro B, El Hajji M, Prange T
Title Urate oxidase from Aspergillus flavus: new crystal-packing contacts in relation to the content of the active site.
Related PDB 1wrr 1ws2 1ws3 1xt4 1xxj 1xy3
Related UniProtKB

Comments
This enzyme is homologous to the counterpart enzyme (S00187 in EzCatDB). The structure of the N-terminal domain has not been determined yet.
According to the literature [9], [18] & [19], this enzyme seems to catalyzes the following reactions:
(A) Oxygenation by O2
(B) Deoxygenation to release H2O2
(C) Additive double-bond deformation by H2O (or hydration)

Created Updated
2004-07-15 2009-04-08