DB code: D00844

RLCP classification 1.30.35885.972 : Hydrolysis
CATH domain 2.60.40.1500 : Immunoglobulin-like
3.20.20.80 : TIM Barrel Catalytic domain
E.C. 3.2.1.37
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.20.20.80 : TIM Barrel S00202 S00210 S00748 S00906 S00907 S00911 S00912 S00915 M00134 M00160 D00479 S00204 S00205 S00206 S00207 S00203 S00208 S00209 S00211 S00213 S00214 M00113 T00307 D00165 D00166 D00169 D00176 D00501 D00502 D00503 D00861 D00864 M00026 M00112 M00193 M00346 T00057 T00062 T00063 T00066 T00067

Uniprot Enzyme Name
UniprotKB Protein name Synonyms CAZy Pfam
P36906 Beta-xylosidase
EC 3.2.1.37
1,4-beta-D-xylan xylohydrolase
Xylan 1,4-beta-xylosidase
GH39 (Glycoside Hydrolase Family 39)
PF01229 (Glyco_hydro_39)
[Graphical View]
Q9ZFM2 Beta-xylosidase
EC 3.2.1.37
1,4-beta-D-xylan xylohydrolase
Xylan 1,4-beta-xylosidase
GH39 (Glycoside Hydrolase Family 39)
PF01229 (Glyco_hydro_39)
[Graphical View]

KEGG enzyme name
Xylan 1,4-beta-xylosidase
Xylobiase
Beta-xylosidase
Exo-1,4-beta-xylosidase
Beta-D-xylopyranosidase
Beta-xylosidase
Beta-xylosidase
Exo-1,4-xylosidase
Exo-1,4-beta-D-xylosidase
1,4-beta-D-xylan xylohydrolase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
Q9ZFM2 XYNB_BACST Hydrolysis of 1,4-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.
P36906 XYNB_THESA Hydrolysis of 1,4-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.

KEGG Pathways
Map code Pathways E.C.
MAP00520 Nucleotide sugars metabolism
MAP00500 Starch and sucrose metabolism

Compound table
Substrates Products Intermediates
KEGG-id L00052 C02352 C02337 C00001 L00052 C02352 C02337 C02096 I00061
E.C.
Compound Xylan 1,4-beta-D-Xylan beta-D-Xyloside H2O Xylan 1,4-beta-D-Xylan beta-D-Xyloside beta-D-Xylose Peptidyl-Glu-D-xylose
Type aromatic ring (only carbon atom),carboxyl group,polysaccharide polysaccharide polysaccharide H2O aromatic ring (only carbon atom),carboxyl group,polysaccharide polysaccharide polysaccharide carbohydrate
ChEBI 15377
28161
PubChem 22247451
962
125409
1px8A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1px8B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1uhvA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1uhvB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1uhvC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1uhvD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91E01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91F01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91G01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91H01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bfgA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bfgB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bfgC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bfgD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bfgE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bfgF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bfgG01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bfgH01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9E01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9F01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9G01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9H01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1px8A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:XYP Unbound
1px8B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Bound:XYP Unbound
1uhvA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:DFX
1uhvB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:DFX
1uhvC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:DFX
1uhvD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:DFX
1w91A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91E02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91F02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91G02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1w91H02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bfgA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ANX-XYP Unbound Unbound Unbound Unbound Intermediate-bound:XYS
2bfgB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-bound:XYS
2bfgC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-bound:XYS
2bfgD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:ANX-XYP Unbound Unbound Unbound Unbound Intermediate-bound:XYS
2bfgE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-bound:XYS
2bfgF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-bound:XYS
2bfgG02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-bound:XYS
2bfgH02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-bound:XYS
2bs9A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9E02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9F02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9G02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2bs9H02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Literature [5], [6], [7] & Swiss-prot;P36906, Q9ZFM2

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1px8A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1px8B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1uhvA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1uhvB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1uhvC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1uhvD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1w91A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1w91B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1w91C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1w91D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1w91E01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1w91F01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1w91G01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1w91H01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bfgA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bfgB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bfgC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bfgD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bfgE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bfgF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bfgG01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bfgH01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bs9A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bs9B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bs9C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bs9D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bs9E01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bs9F01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bs9G01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2bs9H01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1px8A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 277
1px8B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 277
1uhvA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 277
1uhvB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 277
1uhvC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 277
1uhvD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 277
1w91A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
1w91B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
1w91C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
1w91D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
1w91E02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
1w91F02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
1w91G02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
1w91H02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
2bfgA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;;HIS 228;TYR 230;GLU 278 mutant E160A
2bfgB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;;HIS 228;TYR 230;GLU 278 mutant E160A
2bfgC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;;HIS 228;TYR 230;GLU 278 mutant E160A
2bfgD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;;HIS 228;TYR 230;GLU 278 mutant E160A
2bfgE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;;HIS 228;TYR 230;GLU 278 mutant E160A
2bfgF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;;HIS 228;TYR 230;GLU 278 mutant E160A
2bfgG02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;;HIS 228;TYR 230;GLU 278 mutant E160A
2bfgH02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;;HIS 228;TYR 230;GLU 278 mutant E160A
2bs9A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
2bs9B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
2bs9C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
2bs9D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
2bs9E02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
2bs9F02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
2bs9G02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278
2bs9H02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 52;ASN 159;GLU 160;HIS 228;TYR 230;GLU 278

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[5]
Scheme 1
[6]
Figure 5, p.159-162
[7]
Figure 4, p.841-844

References
[1]
Resource
Comments CHARACTERIZATION.
Medline ID
PubMed ID 8612648
Journal Eur J Biochem
Year 1996
Volume 236
Pages 706-13
Authors Armand S, Vieille C, Gey C, Heyraud A, Zeikus JG, Henrissat B
Title Stereochemical course and reaction products of the action of beta-xylosidase from Thermoanaerobacterium saccharolyticum strain B6A-RI.
Related PDB
Related UniProtKB
[2]
Resource
Comments ACTIVE SITE GLU-277, MASS SPECTROMETRY.
Medline ID
PubMed ID 9761746
Journal Biochem J
Year 1998
Volume 335
Pages 449-55
Authors Vocadlo DJ, MacKenzie LF, He S, Zeikus GJ, Withers SG
Title Identification of glu-277 as the catalytic nucleophile of Thermoanaerobacterium saccharolyticum beta-xylosidase using electrospray MS.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 11322958
Journal FEBS Lett
Year 2001
Volume 495
Pages 115-9
Authors Bravman T, Mechaly A, Shulami S, Belakhov V, Baasov T, Shoham G, Shoham Y
Title Glutamic acid 160 is the acid-base catalyst of beta-xylosidase from Bacillus stearothermophilus T-6: a family 39 glycoside hydrolase.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 12146939
Journal Biochemistry
Year 2002
Volume 41
Pages 9736-46
Authors Vocadlo DJ, Wicki J, Rupitz K, Withers SG
Title A case for reverse protonation: identification of Glu160 as an acid/base catalyst in Thermoanaerobacterium saccharolyticum beta-xylosidase and detailed kinetic analysis of a site-directed mutant.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 12146938
Journal Biochemistry
Year 2002
Volume 41
Pages 9727-35
Authors Vocadlo DJ, Wicki J, Rupitz K, Withers SG
Title Mechanism of Thermoanaerobacterium saccharolyticum beta-xylosidase: kinetic studies.
Related PDB
Related UniProtKB
[6]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS)
Medline ID
PubMed ID 14659747
Journal J Mol Biol
Year 2004
Volume 335
Pages 155-65
Authors Yang JK, Yoon HJ, Ahn HJ, Lee BI, Pedelacq JD, Liong EC, Berendzen J, Laivenieks M, Vieille C, Zeikus GJ, Vocadlo DJ, Withers SG, Suh SW
Title Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase.
Related PDB 1px8 1uhv
Related UniProtKB P36906
[7]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS)
Medline ID
PubMed ID 16212978
Journal J Mol Biol
Year 2005
Volume 353
Pages 838-46
Authors Czjzek M, Ben David A, Bravman T, Shoham G, Henrissat B, Shoham Y
Title Enzyme-substrate complex structures of a GH39 beta-xylosidase from Geobacillus stearothermophilus.
Related PDB 2bfg 2bs9
Related UniProtKB Q9ZFM2

Comments
This enzyme belongs to glycosidase family-39, with a retaining mechanism.
According to the literature [6] and [7], the reaction proceeds as follows:
(0) Arg52 and Tyr230 modulate the activity of catalytic nucleophile, Glu277, whereas His228 modulates the activity of a general acid-base, Glu160.
(1) Glu160 acts as a general acid to protonate the leaving oxygen, O4 atom at subsite +1, forming an oxocarbenium-ion-like transition-state. The transition-state is stabilized by Asn159 and Tyr230 through hydrogen bonding to the oxygen atoms of xylose at subsite -1. (SN1-like reaction)
(2) Glu277 makes a nucleophilic attack on C1 atom of substrate, xylan, to form a covalent intermediate with a xylose unit at the non-reducing end (or subsite -1).
(3) Glu160 acts as a general base to deprotonate a water molecule, to activate it.
(4) The activated water makes a nucleophilic attack on the C1 atom of the covalent intermediate. Thus, the substitution reaction completes.

Created Updated
2010-04-19 2012-01-27