DB code: D00864

RLCP classification 1.30.35885.972 : Hydrolysis
CATH domain 3.20.20.80 : TIM Barrel Catalytic domain
2.60.40.1180 : Immunoglobulin-like
E.C. 3.2.1.45
CSA 2f61
M-CSA 2f61
MACiE

CATH domain Related DB codes (homologues)
3.20.20.80 : TIM Barrel S00202 S00210 S00748 S00906 S00907 S00911 S00912 S00915 M00134 M00160 D00479 S00204 S00205 S00206 S00207 S00203 S00208 S00209 S00211 S00213 S00214 M00113 T00307 D00165 D00166 D00169 D00176 D00501 D00502 D00503 D00844 D00861 M00026 M00112 M00193 M00346 T00057 T00062 T00063 T00066 T00067
2.60.40.1180 : Immunoglobulin-like M00113 T00307 D00165 D00176 D00664 D00665 D00863 M00112 M00193 M00314 T00057 T00062 T00067

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq CAZy Pfam
Q9BDT0 Glucosylceramidase
EC 3.2.1.45
Acid beta-glucosidase
Beta-glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
NP_001008997.1 (Protein)
NM_001008997.1 (DNA/RNA sequence)
GH30 (Glycoside Hydrolase Family 30)
PF02055 (Glyco_hydro_30)
[Graphical View]
P04062 Glucosylceramidase
EC 3.2.1.45
Acid beta-glucosidase
Alglucerase
Beta-glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
Imiglucerase
NP_000148.2 (Protein)
NM_000157.3 (DNA/RNA sequence)
NP_001005741.1 (Protein)
NM_001005741.2 (DNA/RNA sequence)
NP_001005742.1 (Protein)
NM_001005742.2 (DNA/RNA sequence)
GH30 (Glycoside Hydrolase Family 30)
PF02055 (Glyco_hydro_30)
[Graphical View]

KEGG enzyme name
Glucosylceramidase
Psychosine hydrolase
Glucosphingosine glucosylhydrolase
GlcCer-beta-glucosidase
beta-D-Glucocerebrosidase
Glucosylcerebrosidase
beta-Glucosylceramidase
Ceramide glucosidase
Glucocerebrosidase
Glucosylsphingosine beta-glucosidase
Glucosylsphingosine beta-D-glucosidase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P04062 GLCM_HUMAN D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine. Interacts with saposin-C. Lysosome membrane, Peripheral membrane protein, Lumenal side. Note=Interaction with saposin-C promotes membrane association.
Q9BDT0 GLCM_PANTR D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine. Interacts with SCARB2 (By similarity). Lysosome membrane, Peripheral membrane protein (By similarity). Note=Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2 (By similarity).

KEGG Pathways
Map code Pathways E.C.
MAP01032 Glycan structures - degradation
MAP00600 Sphingolipid metabolism

Compound table
Substrates Products Intermediates
KEGG-id C01190 C00001 C00031 C00195 I00122
E.C.
Compound D-glucosyl-N-acylsphingosine H2O D-glucose N-acylsphingosine Peptidyl-Glu-Glucose
Type amide group,carbohydrate,lipid H2O carbohydrate amide group,carbohydrate,lipid
ChEBI 15377
4167
PubChem 22247451
962
5793
2vt0A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Intermediate-analogue:CBU
2vt0B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Intermediate-analogue:CBU
1ogsA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ogsB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1y7vA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Intermediate-analogue:INS
1y7vB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Intermediate-analogue:INS
2f61A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2f61B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2j25A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2j25B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nsxA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nsxB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:IFM Unbound Unbound
2nsxC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nsxD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:IFM Unbound Unbound
2nt0A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt0B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt0C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt0D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt1A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt1B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt1C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt1D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2v3dA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:NBV Unbound Unbound Unbound
2v3dB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:NBV Unbound Unbound Unbound
2v3eA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:NND Unbound Unbound Unbound
2v3eB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:NND Unbound Unbound Unbound
2v3fA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2v3fB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2wcgA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:MT5 Unbound Unbound
2wcgB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:MT5 Unbound Unbound
2wklA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2wklB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2xwdA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:LGS Unbound Unbound
2xwdB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:LGS Unbound Unbound
2xweA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:AMF Unbound Unbound Unbound
2xweB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:AMF Unbound Unbound Unbound
3gxdA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxdB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxdC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxdD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxfA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxfB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:IFM Unbound Unbound
3gxfC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxfD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:IFM Unbound Unbound
3gxiA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxiB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxiC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxiD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxmA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxmB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxmC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxmD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3ke0A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3ke0B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3kehA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3kehB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2vt0A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2vt0B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ogsA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1ogsB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1y7vA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
1y7vB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2f61A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2f61B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2j25A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2j25B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nsxA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nsxB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nsxC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nsxD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt0A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt0B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt0C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt0D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt1A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt1B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt1C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2nt1D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2v3dA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2v3dB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2v3eA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2v3eB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2v3fA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2v3fB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2wcgA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2wcgB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2wklA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2wklB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2xwdA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2xwdB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2xweA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
2xweB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxdA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxdB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxdC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxdD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxfA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxfB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxfC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxfD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxiA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxiB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxiC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxiD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxmA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxmB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxmC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3gxmD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3ke0A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3ke0B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3kehA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound
3kehB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
literature [5], [7]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
2vt0A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2vt0B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
1ogsA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
1ogsB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
1y7vA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
1y7vB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2f61A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2f61B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2j25A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2j25B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nsxA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nsxB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nsxC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nsxD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nt0A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nt0B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nt0C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nt0D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nt1A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nt1B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nt1C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2nt1D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2v3dA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2v3dB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2v3eA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2v3eB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2v3fA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2v3fB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2wcgA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2wcgB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2wklA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2wklB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2xwdA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2xwdB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2xweA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
2xweB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxdA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxdB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxdC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxdD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxfA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxfB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxfC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxfD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxiA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxiB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxiC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxiD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxmA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxmB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxmC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3gxmD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340
3ke0A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340 mutant N370S
3ke0B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340 mutant N370S
3kehA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340 mutant N370S
3kehB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 120;ASN 234;GLU 235;HIS 311;TYR 313;GLU 340 mutant N370S
2vt0A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2vt0B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ogsA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ogsB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1y7vA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1y7vB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2f61A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2f61B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2j25A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2j25B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nsxA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nsxB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nsxC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nsxD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nt0A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nt0B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nt0C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nt0D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nt1A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nt1B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nt1C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2nt1D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2v3dA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2v3dB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2v3eA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2v3eB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2v3fA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2v3fB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2wcgA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2wcgB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2wklA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2wklB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2xwdA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2xwdB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2xweA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2xweB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxdA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxdB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxdC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxdD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxfA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxfB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxfC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxfD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxiA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxiB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxiC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxiD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxmA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxmB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxmC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gxmD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3ke0A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3ke0B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3kehA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3kehB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[3]
Fig.1, p.886, p.890
[6]
Fig.1, p.853
[12]
p.4242
[14]
Fig.6, p.29055-29057

References
[1]
Resource
Comments
Medline ID
PubMed ID 3927728
Journal Am J Med Genet
Year 1985
Volume 21
Pages 529-49
Authors Grabowski GA, Goldblatt J, Dinur T, Kruse J, Svennerholm L, Gatt S, Desnick RJ
Title Genetic heterogeneity in Gaucher disease: physicokinetic and immunologic studies of the residual enzyme in cultured fibroblasts from non-neuronopathic and neuronopathic patients.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 1714449
Journal J Biol Chem
Year 1991
Volume 266
Pages 15021-7
Authors Fabbro D, Grabowski GA
Title Human acid beta-glucosidase. Use of inhibitory and activating monoclonal antibodies to investigate the enzyme's catalytic mechanism and saposin A and C binding sites.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 7712292
Journal Curr Opin Struct Biol
Year 1994
Volume 4
Pages 885-92
Authors McCarter JD, Withers SG
Title Mechanisms of enzymatic glycoside hydrolysis.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 8294487
Journal J Biol Chem
Year 1994
Volume 269
Pages 2283-91
Authors Grace ME, Newman KM, Scheinker V, Berg-Fussman A, Grabowski GA
Title Analysis of human acid beta-glucosidase by site-directed mutagenesis and heterologous expression.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 7908905
Journal J Biol Chem
Year 1994
Volume 269
Pages 10975-8
Authors Miao S, McCarter JD, Grace ME, Grabowski GA, Aebersold R, Withers SG
Title Identification of Glu340 as the active-site nucleophile in human glucocerebrosidase by use of electrospray tandem mass spectrometry.
Related PDB
Related UniProtKB P04062
[6]
Resource
Comments
Medline ID
PubMed ID 8535779
Journal Structure
Year 1995
Volume 3
Pages 853-9
Authors Davies G, Henrissat B
Title Structures and mechanisms of glycosyl hydrolases.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 11087714
Journal Glycobiology
Year 2000
Volume 10
Pages 1217-24
Authors Fabrega S, Durand P, Codogno P, Bauvy C, Delomenie C, Henrissat B, Martin BM, McKinney C, Ginns EI, Mornon JP, Lehn P
Title Human glucocerebrosidase: heterologous expression of active site mutants in murine null cells.
Related PDB
Related UniProtKB
[8]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 40-536, GLYCOSYLATION AT ASN-58, AND DISULFIDE BONDS.
Medline ID
PubMed ID 12792654
Journal EMBO Rep
Year 2003
Volume 4
Pages 704-9
Authors Dvir H, Harel M, McCarthy AA, Toker L, Silman I, Futerman AH, Sussman JL
Title X-ray structure of human acid-beta-glucosidase, the defective enzyme in Gaucher disease.
Related PDB 1ogs
Related UniProtKB P04062
[9]
Resource
Comments
Medline ID
PubMed ID 15019270
Journal Trends Pharmacol Sci
Year 2004
Volume 25
Pages 147-51
Authors Futerman AH, Sussman JL, Horowitz M, Silman I, Zimran A
Title New directions in the treatment of Gaucher disease.
Related PDB
Related UniProtKB
[10]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 40-536 IN COMPLEX WITH SYNTHETIC INHIBITOR, ACTIVE SITE.
Medline ID
PubMed ID 15817452
Journal J Biol Chem
Year 2005
Volume 280
Pages 23815-9
Authors Premkumar L, Sawkar AR, Boldin-Adamsky S, Toker L, Silman I, Kelly JW, Futerman AH, Sussman JL
Title X-ray structure of human acid-beta-glucosidase covalently bound to conduritol-B-epoxide. Implications for Gaucher disease.
Related PDB 1y7v
Related UniProtKB P04062
[11]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 40-536, AND GLYCOSYLATION AT ASN-58; ASN-98 AND ASN-185.
Medline ID
PubMed ID 17139081
Journal Acta Crystallogr D Biol Crystallogr
Year 2006
Volume 62
Pages 1458-65
Authors Brumshtein B, Wormald MR, Silman I, Futerman AH, Sussman JL
Title Structural comparison of differently glycosylated forms of acid-beta-glucosidase, the defective enzyme in Gaucher disease.
Related PDB 2j25
Related UniProtKB P04062
[12]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 40-536, CHARACTERIZATION OF GD VARIANTS SER-55; GLN-87; ASN-118; GLN-159; LEU-161; VAL-162; VAL-166; ASN-200; PHE-213; PHE-224; GLU-232; GLU-237; LEU-298; ILE-303; CYS-343; ILE-362; LYS-365; GLY-381; LYS-388; TRP-392; CYS-402; SER-409; VAL-410; HIS-419; LYS-421; ARG-429; LEU-433; SER-436; ASN-438; HIS-448; VAL-455; PRO-483; PRO-500 AND PRO-502, AND MUTAGENESIS OF CYS-43; CYS-57 AND CYS-62.
Medline ID
PubMed ID 16293621
Journal J Biol Chem
Year 2006
Volume 281
Pages 4242-53
Authors Liou B, Kazimierczuk A, Zhang M, Scott CR, Hegde RS, Grabowski GA
Title Analyses of variant acid beta-glucosidases: effects of Gaucher disease mutations.
Related PDB 2f61
Related UniProtKB P04062
[13]
Resource
Comments
Medline ID
PubMed ID 17894779
Journal FEBS J
Year 2007
Volume 274
Pages 4944-50
Authors Yu Z, Sawkar AR, Kelly JW
Title Pharmacologic chaperoning as a strategy to treat Gaucher disease.
Related PDB
Related UniProtKB
[14]
Resource
Comments X-RAY CRYSTALLOGRAPHY
Medline ID
PubMed ID 17666401
Journal J Biol Chem
Year 2007
Volume 282
Pages 29052-8
Authors Brumshtein B, Greenblatt HM, Butters TD, Shaaltiel Y, Aviezer D, Silman I, Futerman AH, Sussman JL
Title Crystal structures of complexes of N-butyl- and N-nonyl-deoxynojirimycin bound to acid beta-glucosidase: insights into the mechanism of chemical chaperone action in Gaucher disease.
Related PDB 2v3e 2v3d
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 17713797
Journal J Mol Model
Year 2007
Volume 13
Pages 1133-9
Authors Zubrzycki IZ, Borcz A, Wiacek M, Hagner W
Title The studies on substrate, product and inhibitor binding to a wild-type and neuronopathic form of human acid-beta-glucosidase.
Related PDB
Related UniProtKB
[16]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 40-536 IN COMPLEXES WITH ISOFAGOMINE, AND SUBCELLULAR LOCATION.
Medline ID
PubMed ID 17187079
Journal Nat Chem Biol
Year 2007
Volume 3
Pages 101-7
Authors Lieberman RL, Wustman BA, Huertas P, Powe AC Jr, Pine CW, Khanna R, Schlossmacher MG, Ringe D, Petsko GA
Title Structure of acid beta-glucosidase with pharmacological chaperone provides insight into Gaucher disease.
Related PDB 2nt0 2nt1 2nsx
Related UniProtKB P04062
[17]
Resource
Comments X-RAY CRYSTALLOGRAPHY
Medline ID
PubMed ID 17524049
Journal Plant Biotechnol J
Year 2007
Volume 5
Pages 579-90
Authors Shaaltiel Y, Bartfeld D, Hashmueli S, Baum G, Brill-Almon E, Galili G, Dym O, Boldin-Adamsky SA, Silman I, Sussman JL, Futerman AH, Aviezer D
Title Production of glucocerebrosidase with terminal mannose glycans for enzyme replacement therapy of Gaucher's disease using a plant cell system.
Related PDB 2v3f
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 18783340
Journal Biol Chem
Year 2008
Volume 389
Pages 1361-9
Authors Kacher Y, Brumshtein B, Boldin-Adamsky S, Toker L, Shainskaya A, Silman I, Sussman JL, Futerman AH
Title Acid beta-glucosidase: insights from structural analysis and relevance to Gaucher disease therapy.
Related PDB 2vt0
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID 19374450
Journal Biochemistry
Year 2009
Volume 48
Pages 4816-27
Authors Lieberman RL, D'aquino JA, Ringe D, Petsko GA
Title Effects of pH and iminosugar pharmacological chaperones on lysosomal glycosidase structure and stability.
Related PDB 3gxd 3gxf 3gxi 3gxm
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 19437524
Journal Chembiochem
Year 2009
Volume 10
Pages 1480-5
Authors Brumshtein B, Aguilar-Moncayo M, Garc?a-Moreno MI, Ortiz Mellet C, Garc?a Fern?ndez JM, Silman I, Shaaltiel Y, Aviezer D, Sussman JL, Futerman AH
Title 6-Amino-6-deoxy-5,6-di-N-(N'-octyliminomethylidene)nojirimycin: synthesis, biological evaluation, and crystal structure in complex with acid beta-glucosidase.
Related PDB 2wcg
Related UniProtKB
[21]
Resource
Comments
Medline ID
PubMed ID 20980263
Journal J Biol Chem
Year 2011
Volume 286
Pages 299-308
Authors Wei RR, Hughes H, Boucher S, Bird JJ, Guziewicz N, Van Patten SM, Qiu H, Pan CQ, Edmunds T
Title X-ray and biochemical analysis of N370S mutant human acid beta-glucosidase.
Related PDB 3ke0 3keh
Related UniProtKB
[22]
Resource
Comments
Medline ID
PubMed ID 21483943
Journal Org Biomol Chem
Year 2011
Volume 9
Pages 4160-7
Authors Brumshtein B, Aguilar-Moncayo M, Benito JM, Garc?a Fernandez JM, Silman I, Shaaltiel Y, Aviezer D, Sussman JL, Futerman AH, Ortiz Mellet C
Title Cyclodextrin-mediated crystallization of acid beta-glucosidase in complex with amphiphilic bicyclic nojirimycin analogues.
Related PDB 2xwd 2xwe
Related UniProtKB

Comments
This enzyme belongs to glycosidase family-30, with a retaining mechanism.
This enzyme seems to be distantly related to beta-xylosidase (EC 3.2.1.37; D00844 in EzCatDB) from glycosidase family-39, and the catalytic residues of the counterpart enzyme can be superimposed with the corresponding residues. According to the literature [14], [16] and [18], these residues are located at the active site, along with the nucleophilic residue, Glu340, and general acid-base, Glu235.
The reaction may proceeds as follows:
(0) Arg120 and Tyr313 may modulate the activity of catalytic nucleophile, Glu340, whereas His311 may modulate the activity of a general acid-base, Glu235.
(1) Glu235 acts as a general acid to protonate the leaving oxygen, O4 atom at subsite +1, forming an oxocarbenium-ion-like transition-state. The transition-state is stabilized by Asn234 and Tyr313 through hydrogen bonding to the oxygen atoms of glucosyl group. (SN1-like reaction)
(2) Glu340 makes a nucleophilic attack on C1 atom of substrate, glucose, to form a covalent intermediate with the glucose unit.
(3) Glu235 acts as a general base to deprotonate a water molecule, to activate it.
(4) The activated water makes a nucleophilic attack on the C1 atom of the covalent intermediate. Thus, the substitution reaction completes.

Created Updated
2008-05-13 2018-04-23