DB code: M00021

CATH domain 1.-.-.- :
-.-.-.- :
-.-.-.- :
2.60.40.350 : Immunoglobulin-like
2.60.98.10 : Tick-borne Encephalitis virus Glycoprotein; domain 1
3.30.67.10 : Viral Envelope Glycoprotein; domain 2
3.30.387.10 : Viral Envelope Glycoprotein; domain 3
-.-.-.- :
-.-.-.- :
-.-.-.- :
2.40.10.- : Thrombin, subunit H
2.40.10.- : Thrombin, subunit H
3.-.-.- :
3.40.50.- : Rossmann fold
-.-.-.- :
-.-.-.- :
3.40.50.- : Rossmann fold
3.40.50.- : Rossmann fold
E.C. 3.4.21.91 2.7.7.48
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Contains RefSeq MEROPS Pfam
P14336 Genome polyprotein
None Peptide 2k
Capsid protein C Core protein
prM
Peptide pr
Small envelope protein M Matrix protein
Envelope protein E
Non-structural protein 1
(NS1)
Non-structural protein 2A
(NS2A)
Serine protease subunit NS2B Flavivirin protease NS2B regulatory subunit Non-structural protein 2B
Serine protease NS3
EC 3.4.21.91
EC 3.6.1.15
EC 3.6.4.13
Flavivirin protease NS3 catalytic subunit Non-structural protein 3
Non-structural protein 4A
(NS4A)
Non-structural protein 4B
(NS4B)
RNA-directed RNA polymerase NS5
EC 2.1.1.56
EC 2.1.1.57
EC 2.7.7.48
Non-structural protein 5
NP_043135.1 (Protein)
NC_001672.1 (DNA/RNA sequence)
S07.001 (Serine)
PF01003 (Flavi_capsid)
PF07652 (Flavi_DEAD)
PF02832 (Flavi_glycop_C)
PF00869 (Flavi_glycoprot)
PF01004 (Flavi_M)
PF00948 (Flavi_NS1)
PF01005 (Flavi_NS2A)
PF01002 (Flavi_NS2B)
PF01350 (Flavi_NS4A)
PF01349 (Flavi_NS4B)
PF00972 (Flavi_NS5)
PF01570 (Flavi_propep)
PF01728 (FtsJ)
PF00271 (Helicase_C)
PF00949 (Peptidase_S7)
[Graphical View]

KEGG enzyme name
flavivirin
(EC 3.4.21.91 )
Yellow fever virus (flavivirus) protease
(EC 3.4.21.91 )
NS2B-3 proteinase
(EC 3.4.21.91 )
RNA-directed RNA polymerase
(EC 2.7.7.48 )
RNA nucleotidyltransferase (RNA-directed)
(EC 2.7.7.48 )
RNA nucleotidyltransferase (RNA-directed)
(EC 2.7.7.48 )
RNA-dependent ribonucleate nucleotidyltransferase
(EC 2.7.7.48 )
3D polymerase
(EC 2.7.7.48 )
PB1 proteins
(EC 2.7.7.48 )
PB2 proteins
(EC 2.7.7.48 )
phage f2 replicase
(EC 2.7.7.48 )
polymerase L
(EC 2.7.7.48 )
Q-beta replicase
(EC 2.7.7.48 )
phage f2 replicase
(EC 2.7.7.48 )
ribonucleic acid replicase
(EC 2.7.7.48 )
ribonucleic acid-dependent ribonucleate nucleotidyltransferase
(EC 2.7.7.48 )
ribonucleic acid-dependent ribonucleic acid polymerase
(EC 2.7.7.48 )
ribonucleic replicase
(EC 2.7.7.48 )
ribonucleic synthetase
(EC 2.7.7.48 )
RNA replicase
(EC 2.7.7.48 )
RNA synthetase
(EC 2.7.7.48 )
RNA transcriptase
(EC 2.7.7.48 )
RNA-dependent ribonucleate nucleotidyltransferase
(EC 2.7.7.48 )
RDRP
(EC 2.7.7.48 )
RNA-dependent RNA polymerase
(EC 2.7.7.48 )
RNA-dependent RNA replicase
(EC 2.7.7.48 )
transcriptase
(EC 2.7.7.48 )

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P14336 POLG_TBEVW Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala. NS3 and NS2B form a heterodimer. NS3 is the catalytic subunit, whereas NS2B strongly stimulates the latter (By similarity). Protein C: Virion (Potential). Membrane, Single-pass membrane protein (Potential). Small envelope protein M: Virion (Potential). Membrane, Single-pass membrane protein (Potential). Envelope protein E: Virion (Potential). Membrane, Multi-pass membrane protein (Potential). Non-structural protein 4A: Membrane, Single- pass membrane protein (Potential). Non-structural protein 4B: Membrane, Multi- pass membrane protein (Potential).

KEGG Pathways
Map code Pathways E.C.
MAP00230 Purine metabolism 2.7.7.48

Compound table
Substrates Products Intermediates
KEGG-id C00017 C00201 C00046 C00017 C00012 C00013 C00046
E.C. 3.4.21.91
2.7.7.48
2.7.7.48
3.4.21.91
3.4.21.91
2.7.7.48
2.7.7.48
Compound Protein Nucleoside triphosphate RNA(n) Protein Peptide Pyrophosphate RNA(n+1)
Type peptide/protein nucleotide nucleic acids peptide/protein peptide/protein phosphate group/phosphate ion nucleic acids
ChEBI 29888
PubChem 1023
21961011
1svbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1svbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1svbA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1svbA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1svbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1svbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1svbA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1svbA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis

References
[1]
Resource
Comments
Medline ID
PubMed ID 8591041
Journal Structure
Year 1995
Volume 3
Pages 645-8
Authors Stuart D, Gouet P
Title Viral envelope glycoproteins swing into action.
Related PDB
Related UniProtKB
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 281-680.
Medline ID 95272700
PubMed ID 7753193
Journal Nature
Year 1995
Volume 375
Pages 291-8
Authors Rey FA, Heinz FX, Mandl C, Kunz C, Harrison SC
Title The envelope glycoprotein from tick-borne encephalitis virus at 2 A resolution.
Related PDB 1svb
Related UniProtKB P14336
[3]
Resource
Comments
Medline ID
PubMed ID 11287576
Journal J Virol
Year 2001
Volume 75
Pages 4268-75
Authors Allison SL, Schalich J, Stiasny K, Mandl CW, Heinz FX
Title Mutational evidence for an internal fusion peptide in flavivirus envelope protein E.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 11264392
Journal J Virol
Year 2001
Volume 75
Pages 4002-7
Authors Bhardwaj S, Holbrook M, Shope RE, Barrett AD, Watowich SJ
Title Biophysical characterization and vector-specific antagonist activity of domain III of the tick-borne flavivirus envelope protein.
Related PDB
Related UniProtKB

Comments
This protein (Swiss-prot;P14336) is genome polyprotein from Tick-borne encephalitis virus, which is composed of capsid protein C, matrix protein, major envelope protein E, four non-structural proteins, flavivirin (EC 3.4.21.91), RNA-directed RNA polymerase (EC 2.7.7.48).
However, the catalytic domain of these enzymes have not been determined yet. (The PDB structure (1svb) corresponds to major envelope protein E.)

Created Updated
2002-10-18 2009-02-26