DB code: M00037

CATH domain 3.40.50.20 : Rossmann fold
3.30.1490.20 : Dna Ligase; domain 1
3.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1
3.90.600.10 : Glycinamide Ribonucleotide Synthetase; Chain A, domain 4
E.C. 6.3.4.13
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.30.1490.20 : Dna Ligase; domain 1 T00082 M00035 T00107 T00108
3.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1 T00082 D00298 M00035 M00051 T00107 T00108
3.40.50.20 : Rossmann fold T00082 T00107 T00108

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P15640 Phosphoribosylamine--glycine ligase
EC 6.3.4.13
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
NP_418433.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491455.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF01071 (GARS_A)
PF02843 (GARS_C)
PF02844 (GARS_N)
[Graphical View]

KEGG enzyme name
phosphoribosylamine---glycine ligase
phosphoribosylglycinamide synthetase
glycinamide ribonucleotide synthetase
phosphoribosylglycineamide synthetase
glycineamide ribonucleotide synthetase
2-amino-N-ribosylacetamide 5'-phosphate kinosynthase
5'-phosphoribosylglycinamide synthetase
GAR

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P15640 PUR2_ECOLI ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. Monomer. Binds 1 magnesium or manganese ion per subunit (By similarity).

KEGG Pathways
Map code Pathways E.C.
MAP00230 Purine metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00305 C00002 C03090 C00037 C00008 C00009 C03838
E.C.
Compound Magnesium ATP 5-Phospho-D-ribosylamine Glycine ADP Orthophosphate N1-(5-Phospho-D-ribosyl)glycinamide
Type divalent metal (Ca2+, Mg2+) amine group,nucleotide amine group,carbohydrate,phosphate group/phosphate ion amino acids amine group,nucleotide phosphate group/phosphate ion amide group,amine group,carbohydrate,phosphate group/phosphate ion
ChEBI 18420
15422
37737
15428
57305
16761
26078
PubChem 888
5957
439905
5257127
750
6022
1004
22486802
160913
1gsoA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gsoA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gsoA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1gsoA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1gsoA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gsoA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gsoA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1gsoA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis

References
[1]
Resource
Comments
Medline ID
PubMed ID 3756144
Journal Biochemistry
Year 1986
Volume 25
Pages 4356-65
Authors Schrimsher JL, Schendel FJ, Stubbe J
Title Isolation of a multifunctional protein with aminoimidazole ribonucleotide synthetase, glycinamide ribonucleotide synthetase, and glycinamide ribonucleotide transformylase activities: characterization of aminoimidazole ribonucleotide synthetase.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 8299947
Journal Gene
Year 1993
Volume 137
Pages 195-202
Authors Kan JL, Jannatipour M, Taylor SM, Moran RG
Title Mouse cDNAs encoding a trifunctional protein of de novo purine synthesis and a related single-domain glycinamide ribonucleotide synthetase.
Related PDB
Related UniProtKB
[3]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)
Medline ID 99060041
PubMed ID 9843369
Journal Biochemistry
Year 1998
Volume 37
Pages 15647-62
Authors Wang W, Kappock TJ, Stubbe J, Ealick SE
Title X-ray crystal structure of glycinamide ribonucleotide synthetase from Escherichia coli.
Related PDB 1gso
Related UniProtKB P15640
[4]
Resource
Comments
Medline ID
PubMed ID 10089364
Journal Acta Crystallogr D Biol Crystallogr
Year 1999
Volume 55
Pages 518-21
Authors Weaver TM, Wang W, Ealick SE
Title Purification, crystallization and preliminary X-ray diffraction data from selenomethionine glycinamide ribonucleotide synthetase.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-03-25 2009-02-26