DB code: M00049

RLCP classification 3.113.90000.394 : Transfer
CATH domain 3.10.20.30 : Ubiquitin-like (UB roll)
3.30.980.10 : Threonyl-tRNA Synthetase; Chain A, domain 2
3.30.54.20 : Replication Terminator Protein; Chain A, domain 2
3.30.930.10 : BirA Bifunctional Protein; domain 2 Catalytic domain
3.40.50.800 : Rossmann fold
E.C. 6.1.1.3
CSA 1qf6
M-CSA 1qf6
MACiE

CATH domain Related DB codes (homologues)
3.10.20.30 : Ubiquitin-like (UB roll) M00039 M00042
3.30.930.10 : BirA Bifunctional Protein; domain 2 S00413 D00291 D00293 D00294 D00295 T00113
3.40.50.800 : Rossmann fold D00295

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P0A8M3 Threonyl-tRNA synthetase
EC 6.1.1.3
Threonine--tRNA ligase
ThrRS
NP_416234.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_489981.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF03129 (HGTP_anticodon)
PF02824 (TGS)
PF00587 (tRNA-synt_2b)
PF07973 (tRNA_SAD)
[Graphical View]
Q8NW68 Threonyl-tRNA synthetase
EC 6.1.1.3
Threonine--tRNA ligase
ThrRS
NP_646443.1 (Protein)
NC_003923.1 (DNA/RNA sequence)
PF03129 (HGTP_anticodon)
PF02824 (TGS)
PF00587 (tRNA-synt_2b)
PF07973 (tRNA_SAD)
[Graphical View]

KEGG enzyme name
threonine---tRNA ligase
threonyl-tRNA synthetase
threonyl-transfer ribonucleate synthetase
threonyl-transfer RNA synthetase
threonyl-transfer ribonucleic acid synthetase
threonyl ribonucleic synthetase
threonine-transfer ribonucleate synthetase
threonine translase
threonyl-tRNA synthetase
TRS

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P0A8M3 SYT_ECOLI ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). Homodimer. Cytoplasm. Binds 1 zinc ion per subunit.
Q8NW68 SYT_STAAW ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). Homodimer (By similarity). Cytoplasm. Binds 1 zinc ion per subunit (By similarity).

KEGG Pathways
Map code Pathways E.C.
MAP00260 Glycine, serine and threonine metabolism
MAP00970 Aminoacyl-tRNA biosynthesis

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00038 C00305 C00002 C00188 C01651 C00013 C00020 C02992
E.C.
Compound Zinc Magnesium ATP L-Threonine tRNA(Thr) Pyrophosphate AMP L-Threonyl-tRNA(Thr)
Type heavy metal divalent metal (Ca2+, Mg2+) amine group,nucleotide amino acids,carbohydrate nucleic acids phosphate group/phosphate ion amine group,nucleotide amino acids,carbohydrate,nucleic acids
ChEBI 29105
18420
15422
16857
57926
29888
16027
PubChem 32051
888
5957
6288
6971019
1023
21961011
6083
1qf6A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyqA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyqB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyrA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyrB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qf6A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyqA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyqB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyrA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyrB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qf6A03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyqA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyqB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyrA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyrB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1evkA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1evkB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Bound:THR Unbound Unbound Unbound Unbound Unbound
1evlA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1evlB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1evlC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1evlD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1fyfA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:SSA
1fyfB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:SSA
1kogA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1kogB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1kogC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1kogD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1kogE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1kogF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1kogG01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1kogH01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1qf6A04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Bound:__A_76(chain B) Unbound Bound:AMP Unbound Unbound
1nyqA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1nyqB04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Unbound Unbound Unbound Unbound Unbound Unbound Intermediate-analogue:TSB
1nyrA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Bound:ATP Bound:THR Unbound Unbound Unbound Unbound Unbound
1nyrB04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_ZN Unbound Bound:ATP Bound:THR Unbound Unbound Unbound Unbound Unbound
1evkA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1evkB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1evlA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1evlB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1evlC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1evlD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1fyfA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1fyfB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1kogA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1kogB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1kogC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1kogD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1kogE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1kogF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1kogG02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1kogH02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qf6A05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyqA05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyqB05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyrA05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1nyrB05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P0A8M3 & literature [3] & [13]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1qf6A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyqA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyqB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyrA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyrB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1qf6A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyqA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyqB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyrA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyrB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1qf6A03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyqA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyqB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyrA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyrB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1evkA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1evkB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1evlA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1evlB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1evlC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1evlD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1fyfA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1fyfB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1kogA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1kogB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1kogC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1kogD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1kogE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1kogF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1kogG01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1kogH01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1qf6A04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 363;ARG 375;LYS 465 CYS 334;HIS 385;HIS 511(Zinc binding);GLU 365(Magnesium binding)
1nyqA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 365;ARG 377;LYS 471 CYS 336;HIS 387;HIS 517(Zinc binding);GLU 367(Magnesium binding)
1nyqB04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 365;ARG 377;LYS 471 CYS 336;HIS 387;HIS 517(Zinc binding);GLU 367(Magnesium binding)
1nyrA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 365;ARG 377;LYS 471 CYS 336;HIS 387;HIS 517(Zinc binding);GLU 367(Magnesium binding)
1nyrB04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ARG 365;ARG 377;LYS 471 CYS 336;HIS 387;HIS 517(Zinc binding);GLU 367(Magnesium binding)
1evkA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1evkB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1evlA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1evlB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1evlC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1evlD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1fyfA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1fyfB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kogA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kogB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kogC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kogD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kogE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kogF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kogG02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1kogH02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1qf6A05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyqA05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyqB05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyrA05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1nyrB05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[7]
Fig.1, p.435
[13]
p.206-207

References
[1]
Resource
Comments
Medline ID
PubMed ID 2388270
Journal J Mol Biol
Year 1990
Volume 214
Pages 819-20
Authors Garber MB, Yaremchuk AD, Tukalo MA, Egorova SP, Fomenkova NP, Nikonov SV
Title Crystals of threonyl-tRNA synthetase from Thermus thermophilus. Preliminary crystallographic data.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 7589494
Journal FEBS Lett
Year 1995
Volume 374
Pages 110-2
Authors Cura V, Kern D, Mitschler A, Moras D
Title Crystallization of threonyl-tRNA synthetase from Thermus thermophilus and preliminary crystallographic data.
Related PDB
Related UniProtKB
[3]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
Medline ID 99251535
PubMed ID 10319817
Journal Cell
Year 1999
Volume 97
Pages 371-81
Authors Sankaranarayanan R, Dock-Bregeon AC, Romby P, Caillet J, Springer M, Rees B, Ehresmann C, Ehresmann B, Moras D
Title The structure of threonyl-tRNA synthetase-tRNA(Thr) complex enlightens its repressor activity and reveals an essential zinc ion in the active site.
Related PDB 1qf6
Related UniProtKB P0A8M3
[4]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 11136973
Journal Cell
Year 2000
Volume 103
Pages 877-84
Authors Dock-Bregeon A, Sankaranarayanan R, Romby P, Caillet J, Springer M, Rees B, Francklyn CS, Ehresmann C, Moras D
Title Transfer RNA-mediated editing in threonyl-tRNA synthetase. The class II solution to the double discrimination problem.
Related PDB 1fyf
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 10675344
Journal EMBO J
Year 2000
Volume 19
Pages 749-57
Authors Staker BL, Korber P, Bardwell JC, Saper MA
Title Structure of Hsp15 reveals a novel RNA-binding motif.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 10632708
Journal Eur J Biochem
Year 2000
Volume 267
Pages 379-93
Authors Cura V, Moras D, Kern D
Title Sequence analysis and modular organization of threonyl-tRNA synthetase from Thermus thermophilus and its interrelation with threonyl-tRNA synthetases of other origins.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 10881182
Journal Nat Struct Biol
Year 2000
Volume 7
Pages 435-6
Authors Musier-Forsyth K, Beuning PJ
Title Role of zinc ion in translational accuracy becomes crystal clear.
Related PDB
Related UniProtKB
[8]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 242-642.
Medline ID 20343005
PubMed ID 10881191
Journal Nat Struct Biol
Year 2000
Volume 7
Pages 461-5
Authors Sankaranarayanan R, Dock-Bregeon AC, Rees B, Bovee M, Caillet J, Romby P, Francklyn CS, Moras D
Title Zinc ion mediated amino acid discrimination by threonyl-tRNA synthetase.
Related PDB 1evk 1evl
Related UniProtKB P0A8M3
[9]
Resource
Comments
Medline ID
PubMed ID 11732604
Journal Acta Biochim Pol
Year 2001
Volume 48
Pages 323-35
Authors Sankaranarayanan R, Moras D
Title The fidelity of the translation of the genetic code.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 11953757
Journal Nat Struct Biol
Year 2002
Volume 9
Pages 343-7
Authors Torres-Larios A, Dock-Bregeon AC, Romby P, Rees B, Sankaranarayanan R, Caillet J, Springer M, Ehresmann C, Ehresmann B, Moras D
Title Structural basis of translational control by Escherichia coli threonyl tRNA synthetase.
Related PDB 1kog
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 14690420
Journal Biochemistry
Year 2003
Volume 42
Pages 15102-13
Authors Bovee ML, Pierce MA, Francklyn CS
Title Induced fit and kinetic mechanism of adenylation catalyzed by Escherichia coli threonyl-tRNA synthetase.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 12554667
Journal EMBO J
Year 2003
Volume 22
Pages 668-75
Authors Beebe K, Ribas De Pouplana L, Schimmel P
Title Elucidation of tRNA-dependent editing by a class II tRNA synthetase and significance for cell viability.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 12875846
Journal J Mol Biol
Year 2003
Volume 331
Pages 201-11
Authors Torres-Larios A, Sankaranarayanan R, Rees B, Dock-Bregeon AC, Moras D
Title Conformational movements and cooperativity upon amino acid, ATP and tRNA binding in threonyl-tRNA synthetase.
Related PDB 1nyr
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 12581352
Journal Mol Microbiol
Year 2003
Volume 47
Pages 961-74
Authors Caillet J, Nogueira T, Masquida B, Winter F, Graffe M, Dock-Bregeon AC, Torres-Larios A, Sankaranarayanan R, Westhof E, Ehresmann B, Ehresmann C, Romby P, Springer M
Title The modular structure of Escherichia coli threonyl-tRNA synthetase as both an enzyme and a regulator of gene expression.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the class-IIa aminoacyl-tRNA synthetase family.
Although the tertiary structures with magnesium ions have not been determined yet, Mg2+ ion might be bound to Glu365 (of 1evk), according to the paper [13].
According to the literature [7] & [8], zinc ion is involved in threonine recognition, discriminating it from other amino acids.
According to the literature [13], this enzyme catalyzes two successive transfer reactions. Firstly, it transfers the adenylate from ATP (the first substrate) to the carboxylate of the second substrate, threonine, resulting in the formation of threonyl-adenylate (intermediate) and the release of the inorganic pyrophosphate. Secondly, it transfers the acyl group from the intermediate to the 3'-OH of tRNA(Thr).
The first transfer reaction proceeds as follows (see [13]):
(1) The first substrate, ATP, adopts a bent conformation so that the alpha-phosphate group faces the carboxylate of the threonine.
(2) Arg363 stabilizes the negatively charged groups, the acceptor group (the carboxylate) and the transferred group (apha-phosphate of ATP), by neutralizing the charged groups. A magnesium ion coordinated to Glu365 also stabilizes the leaving group, the beta- and gamma-phosphphate moieties (or pyrophosphate).
(3) The stabilization of the negatively charged groups leads to an in-line nucleophilic attack by the carboxylate group on the alpha-phosphorus atom, by associative mechanism (SN2-like mechanism).
(4) The pentacovalent transition state is stabilized by three basic residues (Arg363, Arg375 & Lys465), and the magnesium ion. Here, the leaving group, the pyrophosphate, is stabilized by the magnesium ion, Arg375 and Lys465.
(5) The leaving group, the inorganic pyrophosphate, leaves the active site, probably together with the two bridging magnesium ions.
The second acyl transfer reaction has not been elucidated yet.

Created Updated
2004-10-25 2009-04-03