DB code: M00138

CATH domain -.-.-.- :
-.-.-.- :
3.90.56.10 : Phenol Hydroxylase P2 Protein
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-.-.-.- :
-.-.-.- :
E.C. 1.14.13.7
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
P19731 Phenol hydroxylase P2 protein
EC 1.14.13.7
Phenol 2-monooxygenase P2 component
PF02406 (MmoB_DmpM)
[Graphical View]

KEGG enzyme name
phenol 2-monooxygenase
phenol hydroxylase
phenol o-hydroxylase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P19731 DMPM_PSEUF Phenol + NADPH + O(2) = catechol + NADP(+) + H(2)O. The multicomponent enzyme phenol hydroxylase is formed by P0, P1, P2, P3, P4 and P5 polypeptides. FAD. Fe(2+).

KEGG Pathways
Map code Pathways E.C.
MAP00361 gamma-Hexachlorocyclohexane degradation
MAP00622 Toluene and xylene degradation
MAP00626 Naphthalene and anthracene degradation

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00016 C00146 C00005 C00007 C00090 C00006 C00001
E.C.
Compound FAD Phenol NADPH O2 Catechol NADP+ H2O
Type amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms),carbohydrate,nucleotide aromatic ring (only carbon atom) amide group,amine group,nucleotide others aromatic ring (only carbon atom) amide group,amine group,nucleotide H2O
ChEBI 16238
15882
16474
15379
26689
27140
18135
18009
15377
PubChem 643975
20488062
996
5884
977
289
5886
22247451
962
1hqiA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1hqiA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis

References
[1]
Resource
Comments
Medline ID
PubMed ID 8145253
Journal J Mol Biol
Year 1994
Volume 238
Pages 128-30
Authors Enroth C, Huang W, Waters S, Neujahr H, Lindqvist Y, Schneider G
Title Crystallization and preliminary X-ray analysis of phenol hydroxylase from Trichosporon cutaneum.
Related PDB
Related UniProtKB
[2]
Resource
Comments STRUCTURE BY NMR
Medline ID 97164903
PubMed ID 9012665
Journal Biochemistry
Year 1997
Volume 36
Pages 495-504
Authors Qian H, Edlund U, Powlowski J, Shingler V, Sethson I
Title Solution structure of phenol hydroxylase protein component P2 determined by NMR spectroscopy.
Related PDB 1hqi
Related UniProtKB P19731

Comments
This enzyme is composed of six different chains, P0(Swiss-prot;P19729), P1(Swiss-prot;P19730), P2(Swiss-prot;P19731), P3(Swiss-prot;P19732), P4(Swiss-prot;P19733) and P5(Swiss-prot;P19734). PDB structre, 1hqi, corresponds to the structure of P2 component.

Created Updated
2004-02-04 2009-02-26