DB code: M00157

RLCP classification 1.13.30120.22 : Hydrolysis
CATH domain -.-.-.- :
2.40.20.10 : Plasminogen Kringle 4
2.40.20.10 : Plasminogen Kringle 4
2.40.20.10 : Plasminogen Kringle 4
2.40.20.10 : Plasminogen Kringle 4
2.40.20.10 : Plasminogen Kringle 4
2.40.10.10 : Thrombin, subunit H Catalytic domain
2.40.10.10 : Thrombin, subunit H Catalytic domain
E.C. 3.4.21.7
CSA 1qrz 1ddj
M-CSA 1qrz 1ddj
MACiE

CATH domain Related DB codes (homologues)
2.40.20.10 : Plasminogen Kringle 4 M00152 M00155
2.40.10.10 : Thrombin, subunit H M00139 D00214 M00167 D00426 M00133 D00428 D00429 D00430 D00431 D00432 D00433 D00434 D00435 M00227 M00209 D00194 D00197 D00211 D00212 D00216 M00212 D00224 D00497 M00217 M00216 D00528 D00848 D00850 D00851 D00852 D00855 M00152 M00155 M00181 M00315 M00316 M00317 M00348 M00349 T00074 T00410 T00411

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Contains RefSeq MEROPS Pfam
P00747 Plasminogen
EC 3.4.21.7
Plasmin heavy chain A
Activation peptide
Angiostatin
Plasmin heavy chain A, short form
Plasmin light chain B
NP_000292.1 (Protein)
NM_000301.3 (DNA/RNA sequence)
S01.233 (Serine)
PF00051 (Kringle)
PF00024 (PAN_1)
PF00089 (Trypsin)
[Graphical View]

KEGG enzyme name
plasmin
fibrinase
fibrinolysin
actase
serum tryptase
thrombolysin

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P00747 PLMN_HUMAN Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa, higher selectivity than trypsin. Converts fibrin into soluble products. Interacts with AMOT and CSPG4 (also true for angiostatin). Secreted.

KEGG Pathways
Map code Pathways E.C.

Compound table
Substrates Products Intermediates
KEGG-id C00001 C00290 C02873 C00045 C00069 I00087 I00085 I00086
E.C.
Compound H2O Fibrin Peptide(Lys-, Arg-) Amino acid Alcohol Peptidyl-tetrahedral intermediate Acyl-enzyme Tetrahedral intermediate
Type H2O amine group,peptide/protein amino acids,amine group,peptide/protein amino acids carbohydrate
ChEBI 15377
5054
PubChem 22247451
962
439199
1ki0A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ceaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ceaB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1cebA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1cebB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1hpjA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1hpkA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pkrA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ki0A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b2iA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i5kA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1i5kB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ki0A03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1krnA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pk4A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pmkA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1pmkB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
2pk4A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
5hpgA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
5hpgB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1bmlA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1bmlB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1buiA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Transition-state-analogue:GLU-GLY-MAI (chain I)
1buiB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ddjA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ddjB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ddjC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ddjD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1l4dA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1l4zA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qrzA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qrzB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qrzC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qrzD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1rjxB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1bmlA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1bmlB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1buiA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1buiB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ddjA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ddjB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ddjC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1ddjD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1l4dA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1l4zA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qrzA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qrzB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qrzC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1qrzD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1rjxB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
PDB;1bml, 1bui, 1qrz & Swiss-prot;P00747

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1ki0A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ceaA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ceaB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1cebA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1cebB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1hpjA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1hpkA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pkrA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1ki0A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1b2iA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant C181T, E182S, C188G
1i5kA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant C4G, E56D, L72Y
1i5kB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant C4G, E56D, L72Y
1ki0A03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant N289E
1krnA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pk4A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pmkA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1pmkB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2pk4A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
5hpgA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
5hpgB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1bmlA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant S741A
1bmlB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant S741A
1buiA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 195
1buiB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 195
1ddjA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant S741A
1ddjB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant S741A
1ddjC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant S741A
1ddjD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant S741A
1l4dA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant S741A
1l4zA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain mutant S741A
1qrzA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 741 mutant V673M
1qrzB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 741 mutant V673M
1qrzC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 741 mutant V673M
1qrzD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 741 mutant V673M
1rjxB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 741 mutant K698M
1bmlA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated)
1bmlB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated)
1buiA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 57;ASP 102 SER 32(Phospholylated)
1buiB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 57;ASP 102 SER 32(Phospholylated)
1ddjA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated)
1ddjB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated)
1ddjC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated)
1ddjD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated)
1l4dA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated)
1l4zA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated)
1qrzA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated) mutant M585Q, M788L
1qrzB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated) mutant M585Q, M788L
1qrzC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated) mutant M585Q, M788L
1qrzD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated) mutant M585Q, M788L
1rjxB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 603;ASP 646 SER 578(Phospholylated)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[49]
p.906, p.907-909

References
[1]
Resource
Comments ACTIVE SITE.
Medline ID 69234739
PubMed ID 4240117
Journal J Biol Chem
Year 1969
Volume 244
Pages 3590-7
Authors Groskopf WR, Summaria L, Robbins KC
Title Studies on the active center of human plasmin. Partial amino acid sequence of a peptide containing the active center serine residue.
Related PDB
Related UniProtKB P00747
[2]
Resource
Comments ACTIVE SITE.
Medline ID 73149248
PubMed ID 4694729
Journal J Biol Chem
Year 1973
Volume 248
Pages 1631-3
Authors Robbins KC, Bernabe P, Arzadon L, Summaria L
Title The primary structure of human plasminogen. II. The histidine loop of human plasmin: light (B) chain active center histidine sequence.
Related PDB
Related UniProtKB P00747
[3]
Resource
Comments VARIANT THROMBOPHILIA THR-620.
Medline ID 83065132
PubMed ID 6216475
Journal Proc Natl Acad Sci U S A
Year 1982
Volume 79
Pages 6132-6
Authors Miyata T, Iwanaga S, Sakata Y, Aoki N
Title Plasminogen Tochigi: inactive plasmin resulting from replacement of alanine-600 by threonine in the active site.
Related PDB
Related UniProtKB P00747
[4]
Resource
Comments
Medline ID
PubMed ID 6311534
Journal Eur J Biochem
Year 1983
Volume 135
Pages 379-91
Authors Llinas M, De Marco A, Hochschwender SM, Laursen RA
Title A 1H-NMR study of isolated domains from human plasminogen. Structural homology between kringles 1 and 4.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 2933077
Journal Biochim Biophys Acta
Year 1985
Volume 832
Pages 215-9
Authors Ascenzi P, Torroni A, Menegatti E, Guarneri M, Amiconi G
Title Catalytic properties of human Lys77-plasmin. A comparative steady-state and pre-steady-state study.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 2982407
Journal Biochim Biophys Acta
Year 1985
Volume 827
Pages 369-80
Authors De Marco A, Laursen RA, Llinas M
Title Proton Overhauser experiments on kringle 4 from human plasminogen. Implications for the structure of the kringles' hydrophobic core.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 4041537
Journal Biophys J
Year 1985
Volume 48
Pages 411-22
Authors De Marco A, Motta A, Llinas M, Laursen RA
Title Macro- and micro-stabilities of the kringle 4 domain from plasminogen. The effect of ligand binding.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 3004350
Journal Arch Biochem Biophys
Year 1986
Volume 244
Pages 727-41
Authors De Marco A, Laursen RA, Llinas M
Title 1H-NMR spectroscopic manifestations of ligand binding to the kringle 4 domain of human plasminogen.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 3803570
Journal FEBS Lett
Year 1986
Volume 209
Pages 111-6
Authors Williams RJ, Esnouf P, Lawrence M, Cederholm-Williams SA
Title The similarities and differences in structures between kringle 1 of prothrombin and kringle 4 of plasminogen.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 3531210
Journal J Biol Chem
Year 1986
Volume 261
Pages 13684-92
Authors Motta A, Laursen RA, Rajan N, Llinas M
Title Proton magnetic resonance study of kringle 1 from human plasminogen. Insights into the domain structure.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 2820478
Journal Biochemistry
Year 1987
Volume 26
Pages 3827-36
Authors Motta A, Laursen RA, Llinas M, Tulinsky A, Park CH
Title Complete assignment of the aromatic proton magnetic resonance spectrum of the kringle 1 domain from human plasminogen: structure of the ligand-binding site.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 3030435
Journal Biochim Biophys Acta
Year 1987
Volume 912
Pages 254-69
Authors Thewes T, Ramesh V, Simplaceanu EL, Llinas M
Title Isolation, purification and 1H-NMR characterization of a kringle 5 domain fragment from human plasminogen.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 2828641
Journal J Mol Biol
Year 1987
Volume 198
Pages 481-98
Authors Ramesh V, Petros AM, Llinas M, Tulinsky A, Park CH
Title Proton magnetic resonance study of lysine-binding to the kringle 4 domain of human plasminogen. The structure of the binding site.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 2954262
Journal Thromb Res
Year 1987
Volume 45
Pages 451-62
Authors Hijikata-Okunomiya A, Okamoto S, Kikumoto R, Tamao Y, Ohkubo K, Tezuka T, Tonomura S, Matsumoto O
Title Similarity and dissimilarity in the stereogeometry of the active sites of thrombin, trypsin, plasmin and glandular kallikrein.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 3338450
Journal Eur J Biochem
Year 1988
Volume 170
Pages 539-48
Authors Mabbutt BC, Williams RJ
Title Two-dimensional 1H-NMR studies of the solution structure of plasminogen kringle 4.
Related PDB
Related UniProtKB
[16]
Resource
Comments
Medline ID
PubMed ID 2841130
Journal Eur J Biochem
Year 1988
Volume 175
Pages 237-49
Authors Thewes T, Ramesh V, Simplaceanu EL, Llinas M
Title Analysis of the aromatic 1H-NMR spectrum of the kringle 5 domain from human plasminogen. Evidence for a conserved kringle fold.
Related PDB
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID 3135547
Journal Proteins
Year 1988
Volume 3
Pages 85-96
Authors Tulinsky A, Park CH, Mao B, Llinas M
Title Lysine/fibrin binding sites of kringles modeled after the structure of kringle 1 of prothrombin.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 2535939
Journal Biochim Biophys Acta
Year 1989
Volume 994
Pages 121-37
Authors De Marco A, Petros AM, Llinas M, Kaptein R, Boelens R
Title Ligand-binding effects on the kringle 4 domain from human plasminogen: a study by laser photo-CIDNP 1H-NMR spectroscopy.
Related PDB
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID 1966798
Journal Blood Coagul Fibrinolysis
Year 1990
Volume 1
Pages 673-9
Authors Mulichak AM, Tulinsky A
Title Structure of the lysine-fibrin binding subsite of human plasminogen kringle 4.
Related PDB
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 2105955
Journal J Biol Chem
Year 1990
Volume 265
Pages 3906-15
Authors Thewes T, Constantine K, Byeon IJ, Llinas M
Title Ligand interactions with the kringle 5 domain of plasminogen. A study by 1H NMR spectroscopy.
Related PDB
Related UniProtKB
[21]
Resource
Comments STRUCTURE BY NMR OF 374-461.
Medline ID 90219023
PubMed ID 2157850
Journal J Mol Biol
Year 1990
Volume 212
Pages 541-52
Authors Atkinson RA, Williams RJ
Title Solution structure of the kringle 4 domain from human plasminogen by 1H nuclear magnetic resonance spectroscopy and distance geometry.
Related PDB
Related UniProtKB P00747
[22]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 374-461.
Medline ID 92031502
PubMed ID 1657148
Journal Biochemistry
Year 1991
Volume 30
Pages 10576-88
Authors Mulichak AM, Tulinsky A, Ravichandran KG
Title Crystal and molecular structure of human plasminogen kringle 4 refined at 1.9-A resolution.
Related PDB 1pk4
Related UniProtKB P00747
[23]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 374-461.
Medline ID 92031503
PubMed ID 1657149
Journal Biochemistry
Year 1991
Volume 30
Pages 10589-94
Authors Wu TP, Padmanabhan K, Tulinsky A, Mulichak AM
Title The refined structure of the epsilon-aminocaproic acid complex of human plasminogen kringle 4.
Related PDB 2pk4
Related UniProtKB P00747
[24]
Resource
Comments
Medline ID
PubMed ID 1847112
Journal FEBS Lett
Year 1991
Volume 278
Pages 17-22
Authors Teuten AJ, Smith RA, Dobson CM
Title Domain interactions in human plasminogen studied by proton NMR.
Related PDB
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID 1827215
Journal Thromb Haemost
Year 1991
Volume 65
Pages 67-72
Authors Lu H, Soria C, Li H, Soria J, Lijnen HR, Perrot JY, Caen JP
Title Role of active center and lysine binding sites of plasmin in plasmin-induced platelet activation and disaggregation.
Related PDB
Related UniProtKB
[26]
Resource
Comments
Medline ID
PubMed ID 1533399
Journal J Cell Physiol
Year 1992
Volume 151
Pages 255-61
Authors Ryan TJ, Lai L, Malik AB
Title Plasmin generation induces neutrophil aggregation: dependence on the catalytic and lysine binding sites.
Related PDB
Related UniProtKB
[27]
Resource
Comments
Medline ID
PubMed ID 8218159
Journal Biochemistry
Year 1993
Volume 32
Pages 10936-43
Authors Hoover GJ, Menhart N, Martin A, Warder S, Castellino FJ
Title Amino acids of the recombinant kringle 1 domain of human plasminogen that stabilize its interaction with omega-amino acids.
Related PDB
Related UniProtKB
[28]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8054447
Journal Blood Coagul Fibrinolysis
Year 1994
Volume 5
Pages 157-66
Authors Wu TP, Padmanabhan KP, Tulinsky A
Title The structure of recombinant plasminogen kringle 1 and the fibrin binding site.
Related PDB 1pkr
Related UniProtKB
[29]
Resource
Comments
Medline ID
PubMed ID 8187244
Journal Chem Phys Lipids
Year 1994
Volume 67-68
Pages 43-58
Authors Cox M, Schaller J, Boelens R, Kaptein R, Rickli E, Llinas M
Title Kringle solution structures via NMR: two-dimensional 1H-NMR analysis of horse plasminogen kringle 4.
Related PDB
Related UniProtKB
[30]
Resource
Comments STRUCTURE BY NMR OF 96-184.
Medline ID 94237158
PubMed ID 8181476
Journal Eur J Biochem
Year 1994
Volume 221
Pages 939-49
Authors Rejante MR, Llinas M
Title Solution structure of the epsilon-aminohexanoic acid complex of human plasminogen kringle 1.
Related PDB 1hpj 1hpk
Related UniProtKB P00747
[31]
Resource
Comments STRUCTURE BY NMR OF 96-184.
Medline ID 94237157
PubMed ID 8181475
Journal Eur J Biochem
Year 1994
Volume 221
Pages 927-37
Authors Rejante MR, Llinas M
Title 1H-NMR assignments and secondary structure of human plasminogen kringle 1.
Related PDB
Related UniProtKB P00747
[32]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8069221
Journal Protein Sci
Year 1994
Volume 3
Pages 898-910
Authors Padmanabhan K, Wu TP, Ravichandran KG, Tulinsky A
Title Kringle-kringle interactions in multimer kringle structures.
Related PDB 1pmk
Related UniProtKB
[33]
Resource
Comments
Medline ID
PubMed ID 7793622
Journal Anal Biochem
Year 1995
Volume 226
Pages 225-31
Authors Castro MJ, Kingston IB, Anderson S
Title A spectrophotometric assay for the determination of the catalytic efficiency of plasminogen activators using a slowly hydrolyzed plasmin substrate.
Related PDB
Related UniProtKB
[34]
Resource
Comments
Medline ID
PubMed ID 7711052
Journal Biochim Biophys Acta
Year 1995
Volume 1248
Pages 1-10
Authors Rudd PM, Woods RJ, Wormald MR, Opdenakker G, Downing AK, Campbell ID, Dwek RA
Title The effects of variable glycosylation on the functional activities of ribonuclease, plasminogen and tissue plasminogen activator.
Related PDB
Related UniProtKB
[35]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 102-181.
Medline ID 96180681
PubMed ID 8611560
Journal Biochemistry
Year 1996
Volume 35
Pages 2567-76
Authors Mathews II, Vanderhoff-Hanaver P, Castellino FJ, Tulinsky A
Title Crystal structures of the recombinant kringle 1 domain of human plasminogen in complexes with the ligands epsilon-aminocaproic acid and trans-4-(aminomethyl)cyclohexane-1-carboxylic Acid.
Related PDB 1cea 1ceb
Related UniProtKB P00747
[36]
Resource
Comments STRUCTURE BY NMR OF 183-354.
Medline ID 96194156
PubMed ID 8652577
Journal Biochemistry
Year 1996
Volume 35
Pages 2357-64
Authors Sohndel S, Hu CK, Marti D, Affolter M, Schaller J, Llinas M, Rickli EE
Title Recombinant gene expression and 1H NMR characteristics of the kringle (2 + 3) supermodule: spectroscopic/functional individuality of plasminogen kringle domains.
Related PDB
Related UniProtKB P00747
[37]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.67 ANGSTROMS) OF 376-454
Medline ID
PubMed ID
Journal Acta Crystallogr D Biol Crystallogr
Year 1997
Volume 53
Pages 169-78
Authors Stec B, Yamano A, Whitlow M, Teeter MM
Title Structure of Human Plasminogen Kringle 4 at 1.68 angstrom and 277 K. A Possible Structural Role of Disordered Residues.
Related PDB 1krn
Related UniProtKB P00747
[38]
Resource
Comments PHOSPHORYLATION SITE SER-597.
Medline ID 97345939
PubMed ID 9201958
Journal Biochemistry
Year 1997
Volume 36
Pages 8100-6
Authors Wang H, Prorok M, Bretthauer RK, Castellino FJ
Title Serine-578 is a major phosphorylation locus in human plasma plasminogen.
Related PDB
Related UniProtKB P00747
[39]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) OF 480-563.
Medline ID 98198034
PubMed ID 9521645
Journal Biochemistry
Year 1998
Volume 37
Pages 3258-71
Authors Chang Y, Mochalkin I, McCance SG, Cheng B, Tulinsky A, Castellino FJ
Title Structure and ligand binding determinants of the recombinant kringle 5 domain of human plasminogen.
Related PDB 5hpg
Related UniProtKB P00747
[40]
Resource
Comments
Medline ID
PubMed ID 9563511
Journal FEBS Lett
Year 1998
Volume 425
Pages 448-52
Authors Takeda-Shitaka M, Umeyama H
Title Elucidation of the cause for reduced activity of abnormal human plasmin containing an Ala55-Thr mutation: importance of highly conserved Ala55 in serine proteases.
Related PDB
Related UniProtKB
[41]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9783753
Journal Nat Struct Biol
Year 1998
Volume 5
Pages 917-23
Authors Parry MA, Fernandez-Catalan C, Bergner A, Huber R, Hopfner KP, Schlott B, Guhrs KH, Bode W
Title The ternary microplasmin-staphylokinase-microplasmin complex is a proteinase-cofactor-substrate complex in action.
Related PDB 1bui
Related UniProtKB
[42]
Resource
Comments
Medline ID
PubMed ID 9761475
Journal Protein Sci
Year 1998
Volume 7
Pages 1947-59
Authors An SS, Marti DN, Carreno C, Albericio F, Schaller J, Llinas M
Title Structural/functional properties of the Glu1-HSer57 N-terminal fragment of human plasminogen: conformational characterization and interaction with kringle domains.
Related PDB
Related UniProtKB
[43]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9733510
Journal Science
Year 1998
Volume 281
Pages 1662-5
Authors Wang X, Lin X, Loy JA, Tang J, Zhang XC
Title Crystal structure of the catalytic domain of human plasmin complexed with streptokinase.
Related PDB 1bml
Related UniProtKB
[44]
Resource
Comments NMR Structure
Medline ID
PubMed ID 10625440
Journal Biochemistry
Year 1999
Volume 38
Pages 15741-55
Authors Marti DN, Schaller J, Llinas M
Title Solution structure and dynamics of the plasminogen kringle 2-AMCHA complex: 3(1)-helix in homologous domains.
Related PDB 1b2i
Related UniProtKB
[45]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10460175
Journal Biochemistry
Year 1999
Volume 38
Pages 11180-8
Authors Peisach E, Wang J, de los Santos T, Reich E, Ringe D
Title Crystal structure of the proenzyme domain of plasminogen.
Related PDB 1qrz
Related UniProtKB
[46]
Resource
Comments
Medline ID
PubMed ID 10390345
Journal J Mol Biol
Year 1999
Volume 290
Pages 471-9
Authors Jespers L, Lijnen HR, Vanwetswinkel S, Van Hoef B, Brepoels K, Collen D, De Maeyer M
Title Guiding a docking mode by phage display: selection of correlated mutations at the staphylokinase-plasmin interface.
Related PDB
Related UniProtKB
[47]
Resource
Comments
Medline ID
PubMed ID 11076540
Journal Biochemistry
Year 2000
Volume 39
Pages 13974-81
Authors Boxrud PD, Bock PE
Title Streptokinase binds preferentially to the extended conformation of plasminogen through lysine binding site and catalytic domain interactions.
Related PDB
Related UniProtKB
[48]
Resource
Comments
Medline ID
PubMed ID 10769130
Journal Biochemistry
Year 2000
Volume 39
Pages 4740-5
Authors Lin LF, Houng A, Reed GL
Title Epsilon amino caproic acid inhibits streptokinase-plasminogen activator complex formation and substrate binding through kringle-dependent mechanisms.
Related PDB
Related UniProtKB
[49]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10656799
Journal J Mol Biol
Year 2000
Volume 295
Pages 903-14
Authors Wang X, Terzyan S, Tang J, Loy JA, Lin X, Zhang XC
Title Human plasminogen catalytic domain undergoes an unusual conformational change upon activation.
Related PDB 1ddj
Related UniProtKB
[50]
Resource
Comments
Medline ID
PubMed ID 10926513
Journal J Mol Biol
Year 2000
Volume 301
Pages 333-47
Authors Zajicek J, Chang Y, Castellino FJ
Title The effects of ligand binding on the backbone dynamics of the kringle 1 domain of human plasminogen.
Related PDB
Related UniProtKB
[51]
Resource
Comments
Medline ID
PubMed ID 11152303
Journal J Pept Res
Year 2000
Volume 56
Pages 438-45
Authors Schenone MM, Warder SE, Martin JA, Prorok M, Castellino FJ
Title An internal histidine residue from the bacterial surface protein, PAM, mediates its binding to the kringle-2 domain of human plasminogen.
Related PDB
Related UniProtKB
[52]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 11350170
Journal J Mol Biol
Year 2001
Volume 308
Pages 705-19
Authors Rios-Steiner JL, Schenone M, Mochalkin I, Tulinsky A, Castellino FJ
Title Structure and binding determinants of the recombinant kringle-2 domain of human plasminogen to an internal peptide from a group A Streptococcal surface protein.
Related PDB 1i5k
Related UniProtKB
[53]
Resource
Comments
Medline ID
PubMed ID 12080056
Journal J Biol Chem
Year 2002
Volume 277
Pages 33068-74
Authors Turner RB, Liu L, Sazonova IY, Reed GL
Title Structural elements that govern the substrate specificity of the clot-dissolving enzyme plasmin.
Related PDB
Related UniProtKB
[54]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12054798
Journal J Mol Biol
Year 2002
Volume 318
Pages 1009-17
Authors Abad MC, Arni RK, Grella DK, Castellino FJ, Tulinsky A, Geiger JH
Title The X-ray crystallographic structure of the angiogenesis inhibitor angiostatin.
Related PDB 1ki0
Related UniProtKB
[55]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12456874
Journal Protein Eng
Year 2002
Volume 15
Pages 753-61
Authors Wakeham N, Terzyan S, Zhai P, Loy JA, Tang J, Zhang XC
Title Effects of deletion of streptokinase residues 48-59 on plasminogen activation.
Related PDB 1l4d
Related UniProtKB
[56]
Resource
Comments
Medline ID
PubMed ID 12515545
Journal Biochemistry
Year 2003
Volume 42
Pages 114-20
Authors Zhai P, Wakeham N, Loy JA, Zhang XC
Title Functional roles of streptokinase C-terminal flexible peptide in active site formation and substrate recognition in plasminogen activation.
Related PDB
Related UniProtKB
[57]
Resource
Comments
Medline ID
PubMed ID 12773528
Journal J Biol Chem
Year 2003
Volume 278
Pages 30569-77
Authors Sundram V, Nanda JS, Rajagopal K, Dhar J, Chaudhary A, Sahni G
Title Domain truncation studies reveal that the streptokinase-plasmin activator complex utilizes long range protein-protein interactions with macromolecular substrate to maximize catalytic turnover.
Related PDB
Related UniProtKB
[58]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 15211511
Journal Proteins
Year 2004
Volume 56
Pages 277-84
Authors Terzyan S, Wakeham N, Zhai P, Rodgers K, Zhang XC
Title Characterization of Lys-698-to-Met substitution in human plasminogen catalytic domain.
Related PDB 1rjx
Related UniProtKB

Comments
This enzyme is composed of N-terminal PAN domain, five kringle domains and trypsin-like serine protease domain. This enzyme is activated by Streptokinase.
Although this enzyme has got a classical catalytic triad, composed of Ser/His/Asp, which is the same as that of trypsin, its oxyanion hole is not catalytically competent, according to literature [49].
Thus, it should not be included in the same classification with trypsin (D00197 in EzCatDB).

Created Updated
2004-08-20 2011-02-17