DB code: M00310

CATH domain -.-.-.- :
3.30.750.60 : Transcription Regulator spoIIAA
2.120.10.10 : Neuraminidase Catalytic domain
2.40.30.20 : Elongation Factor Tu (Ef-tu); domain 3
4.10.1090.10 : Endosialidase, domain 4
-.-.-.- :
2.-.-.- :
1.-.-.- :
E.C. 3.2.1.129
CSA 1v0e
M-CSA 1v0e
MACiE

CATH domain Related DB codes (homologues)
2.120.10.10 : Neuraminidase D00173 T00064 T00065 T00208

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq CAZy MEROPS Pfam
Q858B1
Endo-N-acetylneuraminidase
Endo-alpha-sialidase
EC 3.2.1.129
Precursor of gp17
EC 3.2.1.129
YP_338127.1 (Protein)
NC_007456.1 (DNA/RNA sequence)
GH58 (Glycoside Hydrolase Family 58)
S74.001 (Serine)
PF12195 (End_beta_barrel)
PF12217 (End_beta_propel)
PF12218 (End_N_terminal)
PF12219 (End_tail_spike)
PF03906 (Phage_T7_tail)
[Graphical View]
Q04830 Endo-N-acetylneuraminidase
Endo-N
EC 3.2.1.129
Endosialidase
G102
GH58 (Glycoside Hydrolase Family 58)
PF12195 (End_beta_barrel)
PF12217 (End_beta_propel)
PF12218 (End_N_terminal)
PF12219 (End_tail_spike)
PF03906 (Phage_T7_tail)
[Graphical View]

KEGG enzyme name
Endo-alpha-sialidase
Endo-N-acylneuraminidase
Endoneuraminidase
Endo-N-acetylneuraminidase
Poly(alpha-2,8-sialosyl) endo-N-acetylneuraminidase
Poly(alpha-2,8-sialoside) alpha-2,8-sialosylhydrolase
Endosialidase
Endo-N

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
Q04830 ENAN_BPK1F Endohydrolysis of (2->8)-alpha-sialosyl linkages in oligo- or poly(sialic) acids. Homotrimer.
Q858B1 Q858B1_BPK1F Endohydrolysis of (2->8)-alpha-sialosyl linkages in oligo- or poly(sialic) acids. Homotrimer.

KEGG Pathways
Map code Pathways E.C.

Compound table
Substrates Products Intermediates
KEGG-id C04466 C00001 L00073 C04466
E.C.
Compound alpha-2,8-Linked polymer of sialic acid H2O beta-sialic acid at reducing end of alpha-2,8-Linked polymer of sialic acid alpha-2,8-Linked polymer of sialic acid
Type amide group,carboxyl group,polysaccharide H2O amide group,carboxyl group,polysaccharide amide group,carboxyl group,polysaccharide
ChEBI 15377
PubChem 22247451
962
1v0eA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvjA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvkA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvkB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvkC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvlA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3ju4A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvjA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvkA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:SLB-SIA-SIA Unbound
3gvkB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:SLB-SIA-SIA Unbound
3gvkC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:SLB-SIA-SIA Unbound
3gvlA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3ju4A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eC03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eD03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eE03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eF03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fC03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fD03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fE03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fF03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvjA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvkA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvkB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvkC03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvlA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3ju4A03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eB04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eC04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eD04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eE04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0eF04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fB04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fC04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fD04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fE04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1v0fF04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvjA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvkA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvkB04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvkC04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gvlA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3ju4A04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6E01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6F01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6E02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6F02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6A03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6B03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6C03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6D03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6E03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6F03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6A04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6B04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6C04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6D04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6E04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
3gw6F04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Literature [6]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1v0eA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvjA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvkA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvkB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvkC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvlA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3ju4A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0eB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0eC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0eD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0eE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0eF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0fA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0fB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0fC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0fD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0fE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0fF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
3gvjA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596; mutant R647A
3gvkA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ;GLU 581;ARG 596;ARG 647 mutant H350A
3gvkB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ;GLU 581;ARG 596;ARG 647 mutant H350A
3gvkC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ;GLU 581;ARG 596;ARG 647 mutant H350A
3gvlA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
3ju4A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain HIS 350;GLU 581;ARG 596;ARG 647
1v0eA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eC03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eD03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eE03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eF03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fC03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fD03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fE03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fF03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvjA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvkA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvkB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvkC03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvlA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3ju4A03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eB04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eC04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eD04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eE04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0eF04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fB04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fC04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fD04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fE04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1v0fF04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvjA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvkA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvkB04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvkC04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gvlA04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3ju4A04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6A01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6B01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6C01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6D01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6E01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6F01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6A02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6B02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6C02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6D02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6E02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6F02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6A03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6B03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6C03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6D03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6E03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6F03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6A04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6B04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6C04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6D04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6E04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3gw6F04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[6]
Fig.3, Fig.4, p.347-348

References
[1]
Resource
Comments
Medline ID
PubMed ID 15608653
Journal Nat Struct Mol Biol
Year 2005
Volume 12
Pages 90-6
Authors Stummeyer K, Dickmanns A, Muhlenhoff M, Gerardy-Schahn R, Ficner R
Title Crystal structure of the polysialic acid-degrading endosialidase of bacteriophage K1F.
Related PDB 1v0e 1v0f
Related UniProtKB Q858B1
[2]
Resource
Comments
Medline ID
PubMed ID 16991177
Journal Chembiochem
Year 2006
Volume 7
Pages 1875-7
Authors Haselhorst T, Stummeyer K, Muhlenhoff M, Schaper W, Gerardy-Schahn R, von Itzstein M
Title Endosialidase NF appears to bind polySia DP5 in a helical conformation.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 18558099
Journal Curr Opin Chem Biol
Year 2008
Volume 12
Pages 539-55
Authors Vocadlo DJ, Davies GJ
Title Mechanistic insights into glycosidase chemistry.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 19411257
Journal J Biol Chem
Year 2009
Volume 284
Pages 17404-10
Authors Morley TJ, Willis LM, Whitfield C, Wakarchuk WW, Withers SG
Title A new sialidase mechanism: bacteriophage K1F endo-sialidase is an inverting glycosidase.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 20124697
Journal Acta Crystallogr D Biol Crystallogr
Year 2010
Volume 66
Pages 176-80
Authors Schulz EC, Neumann P, Gerardy-Schahn R, Sheldrick GM, Ficner R
Title Structure analysis of endosialidase NF at 0.98 A resolution.
Related PDB 3ju4
Related UniProtKB Q04830
[6]
Resource
Comments
Medline ID
PubMed ID 20096705
Journal J Mol Biol
Year 2010
Volume 397
Pages 341-351
Authors Schulz EC, Schwarzer D, Frank M, Stummeyer K, Muhlenhoff M, Dickmanns A, Gerardy-Schahn R, Ficner R
Title Structural Basis for the Recognition and Cleavage of Polysialic Acid by the Bacteriophage K1F Tailspike Protein EndoNF.
Related PDB 3gvj 3gvk 3gvl
Related UniProtKB Q858B1

Comments
This enzyme belongs to glycosidase family-58, with an inverting mechanism.
According to the literature [6], His350 seems to act as a general acid to protonate the leaving group in the SN1-like reaction. On the other hand, it is not still cleare which group can act as a general base to activate the hydrolytic water, substrate carboxylate or Glu581. Moreover, Arg596 and Arg647 may be involved in catalysis.

Created Updated
2010-04-13 2012-02-20