DB code: M00329

RLCP classification 3.103.130000.1162 : Transfer
CATH domain 2.130.10.10 : Methylamine Dehydrogenase; Chain H
3.30.1680.10 : ligand-binding face of the semaphorins, domain 2
2.60.40.10 : Immunoglobulin-like
2.60.40.10 : Immunoglobulin-like
2.60.40.10 : Immunoglobulin-like
2.60.40.10 : Immunoglobulin-like
-.-.-.- :
-.-.-.- :
3.30.200.20 : Phosphorylase Kinase; domain 1
1.10.510.10 : Transferase(Phosphotransferase); domain 1 Catalytic domain
-.-.-.- :
E.C. 2.7.10.1
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
1.10.510.10 : Transferase(Phosphotransferase); domain 1 M00125 M00124 M00131 T00224 M00127 M00129 M00130 M00132 M00136 M00196 M00197 M00198 M00304 M00323 M00325 M00326 M00327 M00328 M00330 M00331 M00332 M00333 M00335 M00339 M00344
2.130.10.10 : Methylamine Dehydrogenase; Chain H S00852 D00039
2.60.40.10 : Immunoglobulin-like M00131 T00257 T00005 M00113 M00127 M00132 M00323 M00325 M00327 M00330 M00331 M00332 T00307 D00166 D00500 M00112 M00193 T00063 T00065 T00067 T00245
3.30.200.20 : Phosphorylase Kinase; domain 1 M00125 M00124 M00131 T00224 M00127 M00129 M00130 M00132 M00136 M00196 M00197 M00198 M00304 M00323 M00325 M00326 M00327 M00328 M00330 M00331 M00332 M00333 M00335 M00339 M00344 D00298

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Contains RefSeq Pfam
Q04912 Macrophage-stimulating protein receptor (MSP receptor) (EC 2.7.10.1) (CDw136) (Protein-tyrosine kinase 8) (p185-Ron)AltName: CD_antigen=CD136;
None Macrophage-stimulating protein receptor alpha chain
Macrophage-stimulating protein receptor beta chain
NP_001231866.1 (Protein)
NM_001244937.1 (DNA/RNA sequence)
NP_002438.2 (Protein)
NM_002447.2 (DNA/RNA sequence)
PF07714 (Pkinase_Tyr)
PF01403 (Sema)
PF01833 (TIG)
[Graphical View]

KEGG enzyme name
Receptor protein-tyrosine kinase
ATP:protein-tyrosine O-phosphotransferase (ambiguous)
MST1R
Protein-tyrosine kinase (ambiguous)
Protein tyrosine kinase (ambiguous)
PTK
Receptor protein tyrosine kinase
RON

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
Q04912 RON_HUMAN ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Heterodimer of an alpha chain and a beta chain which are disulfide linked. Binds PLXNB1. Associates with and is negatively regulated by HYAL2. Interacts when phosphorylated with downstream effectors including PIK3R1, PCLG1, GRB2 and GAB1. Interacts with integrin beta1/ITGB1 in a ligand-independent fashion. Membrane, Single-pass type I membrane protein.

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00305 C00002 C00585 C00008 C01167
E.C.
Compound Mg ATP [protein]-L-tyrosine ADP [protein]-L-tyrosine phosphate
Type divalent metal (Ca2+, Mg2+) amine group,nucleotide aromatic ring (only carbon atom),peptide/protein amine group,nucleotide aromatic ring (only carbon atom),peptide/protein,phosphate group/phosphate ion
ChEBI 18420
15422
16761
PubChem 888
5957
6022
4fwwA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
4fwwA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3plsA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Analogue:ANP Unbound
3plsA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Literature [7] & Swiss-prot;Q04912

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
4fwwA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
4fwwA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3plsA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3plsA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 1208;ARG 1212 ASN 1213;ASP 1226(Magnesium binding) TYR 1238;TYR 1239;TYR 1358 (auto-phosphorylation)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[7]
FIGURE 1

References
[1]
Resource
Comments
Medline ID
PubMed ID 8062829
Journal EMBO J
Year 1994
Volume 13
Pages 3524-32
Authors Gaudino G, Follenzi A, Naldini L, Collesi C, Santoro M, Gallo KA, Godowski PJ, Comoglio PM
Title RON is a heterodimeric tyrosine kinase receptor activated by the HGF homologue MSP.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 7939629
Journal Science
Year 1994
Volume 266
Pages 117-9
Authors Wang MH, Ronsin C, Gesnel MC, Coupey L, Skeel A, Leonard EJ, Breathnach R
Title Identification of the ron gene product as the receptor for the human macrophage stimulating protein.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 10631120
Journal Biochem Biophys Res Commun
Year 2000
Volume 267
Pages 669-75
Authors Xiao ZQ, Chen YQ, Wang MH
Title Requirement of both tyrosine residues 1330 and 1337 in the C-terminal tail of the RON receptor tyrosine kinase for epithelial cell scattering and migration.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 14597639
Journal J Biol Chem
Year 2004
Volume 279
Pages 3726-32
Authors Angeloni D, Danilkovitch-Miagkova A, Miagkov A, Leonard EJ, Lerman MI
Title The soluble sema domain of the RON receptor inhibits macrophage-stimulating protein-induced receptor activation.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 15632155
Journal J Biol Chem
Year 2005
Volume 280
Pages 8893-900
Authors Yokoyama N, Ischenko I, Hayman MJ, Miller WT
Title The C terminus of RON tyrosine kinase plays an autoinhibitory role.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 18950514
Journal J Exp Clin Cancer Res
Year 2008
Volume 27
Pages 55
Authors Lu Y, Yao HP, Wang MH
Title Significance of the entire C-terminus in biological activities mediated by the RON receptor tyrosine kinase and its oncogenic variant RON160.
Related PDB
Related UniProtKB
[7]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.24 ANGSTROMS) OF 1060-1357 IN COMPLEX WITH AMP-PNP AND MAGNESIUM, ACTIVE SITE, PHOSPHORYLATION AT TYR-1238.
Medline ID
PubMed ID 20726546
Journal Biochemistry
Year 2010
Volume 49
Pages 7972-4
Authors Wang J, Steinbacher S, Augustin M, Schreiner P, Epstein D, Mulvihill MJ, Crew AP
Title The crystal structure of a constitutively active mutant RON kinase suggests an intramolecular autophosphorylation hypothesis.
Related PDB 3pls
Related UniProtKB Q04912
[8]
Resource
Comments
Medline ID
PubMed ID 22848655
Journal PLoS One
Year 2012
Volume 7
Pages e41912
Authors Chao KL, Tsai IW, Chen C, Herzberg O
Title Crystal structure of the Sema-PSI extracellular domain of human RON receptor tyrosine kinase.
Related PDB 4fww
Related UniProtKB

Comments
This enzyme is homologous to c-MET (M00322 in EzCatDB).
The catalytic domain of this enzyme is homologous to that of vascular endothelial growth factor receptor (M00131 in EzCatDB).

Created Updated
2011-11-09 2012-12-11