DB code: M00331

RLCP classification 3.103.130000.1162 : Transfer
CATH domain -.-.-.- :
2.60.40.10 : Immunoglobulin-like
2.60.40.10 : Immunoglobulin-like
2.60.40.10 : Immunoglobulin-like
2.60.40.10 : Immunoglobulin-like
2.60.40.10 : Immunoglobulin-like
-.-.-.- :
-.-.-.- :
3.30.200.20 : Phosphorylase Kinase; domain 1
1.10.510.10 : Transferase(Phosphotransferase); domain 1 Catalytic domain
-.-.-.- :
E.C. 2.7.10.1
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
1.10.510.10 : Transferase(Phosphotransferase); domain 1 M00125 M00124 M00131 T00224 M00127 M00129 M00130 M00132 M00136 M00196 M00197 M00198 M00304 M00323 M00325 M00326 M00327 M00328 M00329 M00330 M00332 M00333 M00335 M00339 M00344
2.60.40.10 : Immunoglobulin-like M00131 T00257 T00005 M00113 M00127 M00132 M00323 M00325 M00327 M00329 M00330 M00332 T00307 D00166 D00500 M00112 M00193 T00063 T00065 T00067 T00245
3.30.200.20 : Phosphorylase Kinase; domain 1 M00125 M00124 M00131 T00224 M00127 M00129 M00130 M00132 M00136 M00196 M00197 M00198 M00304 M00323 M00325 M00326 M00327 M00328 M00329 M00330 M00332 M00333 M00335 M00339 M00344 D00298

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P36888 Receptor-type tyrosine-protein kinase FLT3 (EC 2.7.10.1) (FL cytokine receptor) (Fetal liver kinase-2) (FLK-2) (Fms-like tyrosine kinase 3) (FLT-3) (Stem cell tyrosine kinase 1) (STK-1)AltName: CD_antigen=CD135;
None NP_004110.2 (Protein)
NM_004119.2 (DNA/RNA sequence)
PF00047 (ig)
PF07714 (Pkinase_Tyr)
[Graphical View]

KEGG enzyme name
Receptor protein-tyrosine kinase
ATP:protein-tyrosine O-phosphotransferase (ambiguous)
CD135
CDw135
FLK1
FLK2
FLT1
FLT2
FLT3
FLT4
FMS
Fv2
Protein-tyrosine kinase (ambiguous)
Protein tyrosine kinase (ambiguous)
Receptor protein tyrosine kinase
STK
STK1

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P36888 FLT3_HUMAN ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Monomer in the absence of bound FLT3LG. Homodimer in the presence of bound FLT3LG. Interacts with FIZ1 following ligand activation (By similarity). Interacts with FES, FER, LYN, FGR, HCK, SRC and GRB2. Interacts with PTPRJ/DEP-1 and PTPN11/SHP2. Membrane, Single-pass type I membrane protein. Endoplasmic reticulum lumen. Note=Constitutively activated mutant forms with internal tandem duplications are less efficiently transported to the cell surface and a significant proportion is retained in an immature form in the endoplasmic reticulum lumen. The activated kinase is rapidly targeted for degradation.

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00305 C00002 C00585 C00008 C01167
E.C.
Compound Mg ATP [protein]-L-tyrosine ADP [protein]-L-tyrosine phosphate
Type divalent metal (Ca2+, Mg2+) amine group,nucleotide aromatic ring (only carbon atom),peptide/protein amine group,nucleotide aromatic ring (only carbon atom),peptide/protein,phosphate group/phosphate ion
ChEBI 18420
15422
16761
PubChem 888
5957
6022
3qs7E01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7F01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9E01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9G01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9H01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7E02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7F02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7G01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7H01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9E02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9G02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9H02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9F01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7E03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7F03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7G02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7H02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9E03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9G03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9H03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9F02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7E04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7F04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs7H03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9E04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9G04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9H04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9F03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9E05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9G05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9H05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3qs9F04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1rjbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
1rjbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Literature [2] & Swiss-prot;P36888

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
3qs7E01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7F01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9E01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9G01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9H01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7E02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7F02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7G01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7H01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9E02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9G02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9H02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9F01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7E03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7F03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7G02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7H02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9E03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9G03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9H03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9F02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7E04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7F04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs7H03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9E04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9G04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9H04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9F03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9E05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9G05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9H05 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3qs9F04 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1rjbA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1rjbA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 811;ARG 815 ASN 816;ASP 829(Magnesium binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis

References
[1]
Resource
Comments
Medline ID
PubMed ID 10881197
Journal Nat Struct Biol
Year 2000
Volume 7
Pages 486-91
Authors Savvides SN, Boone T, Andrew Karplus P
Title Flt3 ligand structure and unexpected commonalities of helical bundles and cystine knots.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 14759363
Journal Mol Cell
Year 2004
Volume 13
Pages 169-78
Authors Griffith J, Black J, Faerman C, Swenson L, Wynn M, Lu F, Lippke J, Saxena K
Title The structural basis for autoinhibition of FLT3 by the juxtamembrane domain.
Related PDB 1rjb
Related UniProtKB P36888
[3]
Resource
Comments
Medline ID
PubMed ID 18628457
Journal Clin Cancer Res
Year 2008
Volume 14
Pages 4437-45
Authors Vempati S, Reindl C, Wolf U, Kern R, Petropoulos K, Naidu VM, Buske C, Hiddemann W, Kohl TM, Spiekermann K
Title Transformation by oncogenic mutants and ligand-dependent activation of FLT3 wild-type requires the tyrosine residues 589 and 591.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 21389326
Journal Blood
Year 2011
Volume 118
Pages 60-8
Authors Verstraete K, Vandriessche G, Januar M, Elegheert J, Shkumatov AV, Desfosses A, Van Craenenbroeck K, Svergun DI, Gutsche I, Vergauwen B, Savvides SN
Title Structural insights into the extracellular assembly of the hematopoietic Flt3 signaling complex.
Related PDB 3qs7 3qs9
Related UniProtKB P36888

Comments
This enzyme belongs to platelet-derived growth factor (PDGF) receptor family, along with homologous enzymes, such as colony-stimulating factor-1 receptor (CSF-1-R, cFMS) (M00330 in EzCatDB) and tyrosine-protein kinase Kit (Kit) (M00323 in EzCatDB).
The catalytic domain of this enzyme is homologous to that of vascular endothelial growth factor receptor (M00131 in EzCatDB).

Created Updated
2011-11-17 2012-12-05