DB code: M00344

RLCP classification 3.103.130000.1162 : Transfer
CATH domain 2.30.29.30 : PH-domain like
-.-.-.- :
-.-.-.- :
3.30.505.10 : SHC Adaptor Protein
3.30.200.20 : Phosphorylase Kinase; domain 1
1.10.510.10 : Transferase(Phosphotransferase); domain 1 Catalytic domain
E.C. 2.7.10.2
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
1.10.510.10 : Transferase(Phosphotransferase); domain 1 M00125 M00124 M00131 T00224 M00127 M00129 M00130 M00132 M00136 M00196 M00197 M00198 M00304 M00323 M00325 M00326 M00327 M00328 M00329 M00330 M00331 M00332 M00333 M00335 M00339
2.30.29.30 : PH-domain like M00125 M00183 M00118
3.30.200.20 : Phosphorylase Kinase; domain 1 M00125 M00124 M00131 T00224 M00127 M00129 M00130 M00132 M00136 M00196 M00197 M00198 M00304 M00323 M00325 M00326 M00327 M00328 M00329 M00330 M00331 M00332 M00333 M00335 M00339 D00298
3.30.505.10 : SHC Adaptor Protein M00183 M00043 M00130 M00148 T00256 M00304 M00333 M00339 T00221

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P51813 Cytoplasmic tyrosine-protein kinase BMX
EC 2.7.10.2
Bone marrow tyrosine kinase gene in chromosome X protein
Epithelial and endothelial tyrosine kinase
ETK
NTK38
NP_001712.1 (Protein)
NM_001721.6 (DNA/RNA sequence)
NP_975010.1 (Protein)
NM_203281.2 (DNA/RNA sequence)
PF00779 (BTK)
PF00169 (PH)
PF07714 (Pkinase_Tyr)
PF00017 (SH2)
[Graphical View]

KEGG enzyme name
Non-specific protein-tyrosine kinase
ATP:protein-tyrosine O-phosphotransferase (ambiguous)
Bruton's tyrosine kinase
BMX
Cytoplasmic protein tyrosine kinase
ETK
Ntk

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P51813 BMX_HUMAN ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B. Cytoplasm. Note: Localizes to the edges of spreading cells when complexed with BCAR1. Binds 1 zinc ion per subunit (By similarity).

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00305 C00002 C00585 C00008 C01167
E.C.
Compound Magnesium ATP [protein]-L-tyrosine ADP [protein]-L-tyrosine phosphate
Type divalent metal (Ca2+, Mg2+) amine group,nucleotide aromatic ring (only carbon atom),peptide/protein amine group,nucleotide aromatic ring (only carbon atom),peptide/protein,phosphate group/phosphate ion
ChEBI 18420
15422
16761
PubChem 888
5957
6022
2ys2A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
2ekxA00 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3sxrA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3sxrB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3sxsA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3sxrA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3sxrB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound
3sxsA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
2ys2A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
2ekxA00 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3sxrA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3sxrB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3sxsA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
3sxrA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 536;ARG 540 ASN 541;ASP 554(Magnesium binding)
3sxrB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 536;ARG 540 ASN 541;ASP 554(Magnesium binding)
3sxsA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 536;ARG 540 ASN 541;ASP 554(Magnesium binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[1]
Figure 2

References
[1]
Resource
Comments REVIEW ON FUNCTION.
Medline ID
PubMed ID 11114746
Journal Oncogene
Year 2000
Volume 19
Pages 5651-61
Authors Qiu Y, Kung HJ
Title Signaling network of the Btk family kinases.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 11331870
Journal Nat Cell Biol
Year 2001
Volume 3
Pages 439-44
Authors Chen R, Kim O, Li M, Xiong X, Guan JL, Kung HJ, Chen H, Shimizu Y, Qiu Y
Title Regulation of the PH-domain-containing tyrosine kinase Etk by focal adhesion kinase through the FERM domain.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 14532277
Journal J Biol Chem
Year 2003
Volume 278
Pages 51267-76
Authors Zhang R, Xu Y, Ekman N, Wu Z, Wu J, Alitalo K, Min W
Title Etk/Bmx transactivates vascular endothelial growth factor 2 and recruits phosphatidylinositol 3-kinase to mediate the tumor necrosis factor-induced angiogenic pathway.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 18292575
Journal J Immunol
Year 2008
Volume 180
Pages 3485-91
Authors Semaan N, Alsaleh G, Gottenberg JE, Wachsmann D, Sibilia J
Title Etk/BMX, a Btk family tyrosine kinase, and Mal contribute to the cross-talk between MyD88 and FAK pathways.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 21883956
Journal Chem Biol Drug Des
Year 2011
Volume 78
Pages 739-48
Authors Muckelbauer J, Sack JS, Ahmed N, Burke J, Chang CY, Gao M, Tino J, Xie D, Tebben AJ
Title X-ray crystal structure of bone marrow kinase in the x chromosome: a Tec family kinase.
Related PDB 3sxr 3sxs
Related UniProtKB P51813

Comments
This enzyme belongs to Tec family of non-receptor tyrosine kinases.
This enzyme is composed of the N-terminal pleckstrin homology (PH) domain, BTK-type zinc finger motif, an unknown domain, which can be Src homology 3 (SH3) domain, Src homology 2 (SH2) domain, and the C-terminal kinase domain (see [1]).
The catalytic domain of this enzyme is homologous to Proto-oncogene tyrosine-protein kinase ABL1 (M00130 in EzCatDB), with common active-site residues.

Created Updated
2012-06-19 2013-01-30