DB code: S00101

CATH domain 2.40.40.20 : Barwin-like endoglucanases Catalytic domain
E.C. 4.1.1.11
CSA 1aw8
M-CSA 1aw8
MACiE

CATH domain Related DB codes (homologues)
2.40.40.20 : Barwin-like endoglucanases M00007

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Contains RefSeq Pfam
P0A790 Aspartate 1-decarboxylase
EC 4.1.1.11
Aspartate alpha-decarboxylase
Aspartate 1-decarboxylase beta chain
Aspartate 1-decarboxylase alpha chain
NP_414673.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_488434.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF02261 (Asp_decarbox)
[Graphical View]

KEGG enzyme name
aspartate 1-decarboxylase
aspartate alpha-decarboxylase
L-aspartate alpha-decarboxylase
aspartic alpha-decarboxylase
L-aspartate 1-carboxy-lyase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P0A790 PAND_ECOLI L-aspartate = beta-alanine + CO(2). Heterooctamer of four alpha and four beta subunits. Cytoplasm. Pyruvoyl group.

KEGG Pathways
Map code Pathways E.C.
MAP00252 Alanine and aspartate metabolism
MAP00410 beta-Alanine metabolism

Compound table
Substrates Products Intermediates
KEGG-id C00049 C00011 C00099
E.C.
Compound L-Aspartate CO2 beta-Alanine
Type amino acids,carboxyl group others amino acids
ChEBI 17053
16526
16958
57966
PubChem 44367445
5960
280
239
4755801
1aw8A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1aw8D Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1aw8B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1aw8E Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
PDB;1aw8 & Swiss-prot;P0A790 & literature;[1],[2],[5]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1aw8A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 9
1aw8D Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 9
1aw8B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 58 PYR 25(Pyruvoyl Ser)
1aw8E Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain TYR 58 PYR 25(Pyruvoyl Ser)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[1]
Scheme 2, p.312 2
[2]
Fig.1b, p.290-291
[5]
Scheme 1 p.1760

References
[1]
Resource
Comments
Medline ID
PubMed ID 9765870
Journal Biochem Soc Trans
Year 1998
Volume 26
Pages 310-5
Authors Abell C
Title Enzymes that exploit imines--one way or the other.
Related PDB
Related UniProtKB
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
Medline ID 98206295
PubMed ID 9546220
Journal Nat Struct Biol
Year 1998
Volume 5
Pages 289-93
Authors Albert A, Dhanaraj V, Genschel U, Khan G, Ramjee MK, Pulido R, Sibanda BL, von Delft F, Witty M, Blundell TL, Smith AG, Abell C
Title Crystal structure of aspartate decarboxylase at 2.2 A resolution provides evidence for an ester in protein self-processing.
Related PDB 1aw8
Related UniProtKB P0A790
[3]
Resource
Comments
Medline ID
PubMed ID 10368289
Journal Structure Fold Des
Year 1999
Volume 7
Pages 227-36
Authors Castillo RM, Mizuguchi K, Dhanaraj V, Albert A, Blundell TL, Murzin AG
Title A six-stranded double-psi beta barrel is shared by several protein superfamilies.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 10610264
Journal Structure Fold Des
Year 1999
Volume 7
Pages R215-6
Authors Mizuguchi K, Dhanaraj V, Blundell TL, Murzin AG
Title N-ethylmaleimide-sensitive fusion protein (NSF) and CDC48 confirmed as members of the double-psi beta-barrel aspartate decarboxylase/formate dehydrogenase family.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 12240302
Journal Chem Commun (Camb)
Year 2001
Volume (18)
Pages 1760-1
Authors Saldanha SA, Birch LM, Webb ME, Nabbs BK, von Delft F, Smith AG, Abell C
Title Identification of Tyr58 as the proton donor in the aspartate-alpha-decarboxylase reaction.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 12182836
Journal Protein Expr Purif
Year 2002
Volume 25
Pages 533-40
Authors Chopra S, Pai H, Ranganathan A
Title Expression, purification, and biochemical characterization of Mycobacterium tuberculosis aspartate decarboxylase, PanD.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-03-25 2009-02-26