DB code: S00261

RLCP classification 3.747.6300.267 : Transfer
CATH domain 3.40.50.150 : Rossmann fold Catalytic domain
E.C. 2.1.1.72
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.40.50.150 : Rossmann fold S00637 S00639 S00262 S00291 S00412 D00075 D00076 D00079 D00080 D00082 D00083 D00823

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
P14751 Modification methylase RsrI
M.RsrI
EC 2.1.1.72
Adenine-specific methyltransferase RsrI
PF01555 (N6_N4_Mtase)
[Graphical View]
P23192 Modification methylase MboII
M.MboII
EC 2.1.1.72
Adenine-specific methyltransferase MboII
DNA MTase MboIIA
PF01555 (N6_N4_Mtase)
[Graphical View]

KEGG enzyme name
site-specific DNA-methyltransferase (adenine-specific)
modification methylase
restriction-modification system

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P14751 MTR1_RHOSH S-adenosyl-L-methionine + DNA adenine = S- adenosyl-L-homocysteine + DNA 6-methylaminopurine.
P23192 MTM2_MORBO S-adenosyl-L-methionine + DNA adenine = S- adenosyl-L-homocysteine + DNA 6-methylaminopurine. Homodimer.

KEGG Pathways
Map code Pathways E.C.

Compound table
Substrates Products Intermediates
KEGG-id C00019 C00821 C00021 C03391
E.C.
Compound S-Adenosyl-L-methionine DNA adenine S-Adenosyl-L-homocysteine DNA 6-methylaminopurine
Type amino acids,amine group,nucleoside,sulfonium ion amine group,nucleic acids amino acids,amine group,nucleoside,sulfide group amine group,nucleic acids
ChEBI 67040
16680
57856
PubChem 34755
25246222
439155
1eg2A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:MTA Unbound Unbound Unbound
1g60A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:SAM Unbound Unbound Unbound
1g60B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:SAM Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
literature [2]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1eg2A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 65
1g60A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 30
1g60B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 30

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[2]
Fig.1 1

References
[1]
Resource
Comments
Medline ID
PubMed ID 1511884
Journal Gene
Year 1992
Volume 118
Pages 5-11
Authors Kaszubska W, Webb HK, Gumport RI
Title Purification and characterization of the M.RsrI DNA methyltransferase from Escherichia coli.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 11024175
Journal Nucleic Acids Res
Year 2000
Volume 28
Pages 3950-61
Authors Scavetta RD, Thomas CB, Walsh MA, Szegedi S, Joachimiak A, Gumport RI, Churchill ME
Title Structure of RsrI methyltransferase, a member of the N6-adenine beta class of DNA methyltransferases.
Related PDB 1eg2
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 11024176
Journal Nucleic Acids Res
Year 2000
Volume 28
Pages 3962-71
Authors Szegedi SS, Reich NO, Gumport RI
Title Substrate binding in vitro and kinetics of RsrI [N6-adenine] DNA methyltransferase.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 11024177
Journal Nucleic Acids Res
Year 2000
Volume 28
Pages 3972-81
Authors Szegedi SS, Gumport RI
Title DNA binding properties in vivo and target recognition domain sequence alignment analyses of wild-type and mutant RsrI [N6-adenine] DNA methyltransferases.
Related PDB
Related UniProtKB

Comments
This methylase recognizes the double-stranded sequence [G-A-A-T-T-C], causes specific methylation on 6-amino group of Adenine on both strands, and protects the DNA from cleavage by the RsrI endonuclease.
According to the literature [2], Asp65 acts as a general base, which can activate the acceptor group, the amino group of adenine. This activated group in turn makes a nucleophilic attack on the methyl group of the SAM molecule.

Created Updated
2002-09-03 2009-02-26