DB code: S00279

CATH domain 3.40.30.10 : Glutaredoxin Catalytic domain
E.C. 1.11.1.9
CSA 1gp1
M-CSA 1gp1
MACiE

CATH domain Related DB codes (homologues)
3.40.30.10 : Glutaredoxin S00916 M00184 D00866 D00869 D00870 D00278

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P00435 Glutathione peroxidase 1
GPx-1
GSHPx-1
EC 1.11.1.9
Cellular glutathione peroxidase
NP_776501.1 (Protein)
NM_174076.3 (DNA/RNA sequence)
PF00255 (GSHPx)
[Graphical View]

KEGG enzyme name
glutathione peroxidase
GSH peroxidase
selenium-glutathione peroxidase
reduced glutathione peroxidase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P00435 GPX1_BOVIN 2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O. Homotetramer. Cytoplasm.

KEGG Pathways
Map code Pathways E.C.
MAP00590 Arachidonic acid metabolism
MAP00480 Glutathione metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C01529 C00051 C00027 C00127 C00001
E.C.
Compound Selenium Glutathione H2O2 Oxidized glutathione H2O
Type others amino acids,carboxyl group,peptide/protein,sulfhydryl group others amino acids,carboxyl group,peptide/protein,disulfide bond H2O
ChEBI 27568
16856
16240
17858
15377
PubChem 6326970
124886
25246407
22326046
784
11215652
65359
22247451
962
1gp1A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:SEC Unbound Unbound Unbound
1gp1B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:SEC Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1gp1A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SEC 45(Seleninic acid)
1gp1B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SEC 45(Seleninic acid)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[2]
p.66-68, Fig.13
[6]
Fig.1, p.304
[7]
p.216-218
[8]
p.90-91
[9]
p.147-149
[10]
p.878-880

References
[1]
Resource
Comments
Medline ID
PubMed ID 1140308
Journal Experientia
Year 1975
Volume 31
Pages 769-70
Authors Beutler E, Beutler B, Matsumoto J
Title Glutathione peroxidase activity of inorganic selenium and seleno-DL-cysteine.
Related PDB
Related UniProtKB
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)
Medline ID 83209650
PubMed ID 6852035
Journal Eur J Biochem
Year 1983
Volume 133
Pages 51-69
Authors Epp O, Ladenstein R, Wendel A
Title The refined structure of the selenoenzyme glutathione peroxidase at 0.2-nm resolution.
Related PDB 1gp1
Related UniProtKB P00435
[3]
Resource
Comments
Medline ID
PubMed ID 6714945
Journal Hoppe Seylers Z Physiol Chem
Year 1984
Volume 365
Pages 195-212
Authors Gunzler WA, Steffens GJ, Grossmann A, Kim SM, Otting F, Wendel A, Flohe L
Title The amino-acid sequence of bovine glutathione peroxidase.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 6233508
Journal Nutr Rev
Year 1984
Volume 42
Pages 168-9
Authors [No authors listed]
Title Position and function of the organic selenium in glutathione peroxidase.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 2130181
Journal J Toxicol Clin Exp
Year 1990
Volume 10
Pages 375-83
Authors Bompart G
Title Cisplatin-induced changes on cytochrome P-450, lipid peroxidation and some P-450 related specific catalytic activities in rat liver.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 7651210
Journal Methods Enzymol
Year 1995
Volume 251
Pages 303-14
Authors Biewenga GP, Bast A
Title Reaction of lipoic acid with ebselen and hypochlorous acid.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 7766257
Journal Z Naturforsch [C]
Year 1995
Volume 50
Pages 209-19
Authors Prutz WA
Title Glutathione peroxidase-like activity of simple selenium compounds. Peroxides and the heterocyclic N-oxide resazurin acting as O-atom donors.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 8912640
Journal Biochem Biophys Res Commun
Year 1996
Volume 228
Pages 88-93
Authors Hou Y, Guo Z, Li J, Wang PG
Title Seleno compounds and glutathione peroxidase catalyzed decomposition of S-nitrosothiols.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 9315305
Journal Biomed Environ Sci
Year 1997
Volume 10
Pages 136-55
Authors Aumann KD, Bedorf N, Brigelius-Flohe R, Schomburg D, Flohe L
Title Glutathione peroxidase revisited--simulation of the catalytic cycle by computer-assisted molecular modelling.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 9180378
Journal J Mol Biol
Year 1997
Volume 268
Pages 869-85
Authors Ren B, Huang W, Akesson B, Ladenstein R
Title The crystal structure of seleno-glutathione peroxidase from human plasma at 2.9 A resolution.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 9517485
Journal Biol Trace Elem Res
Year 1998
Volume 61
Pages 127-36
Authors Howard SA, Hawkes WC
Title The relative effectiveness of human plasma glutathione peroxidase as a catalyst for the reduction of hydroperoxides by glutathione.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 9828014
Journal Protein Sci
Year 1998
Volume 7
Pages 2465-8
Authors Schroder E, Ponting CP
Title Evidence that peroxiredoxins are novel members of the thioredoxin fold superfamily.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 11018712
Journal Biochim Biophys Acta
Year 2000
Volume 1481
Pages 222-8
Authors Liu J, Luo G, Ren X, Mu Y, Bai Y, Shen J
Title A bis-cyclodextrin diselenide with glutathione peroxidase-like activity.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 10995212
Journal Bioconjug Chem
Year 2000
Volume 11
Pages 682-7
Authors Ren X, Liu J, Luo G, Zhang Y, Luo Y, Yan G, Shen J
Title A novel selenocystine-beta-cyclodextrin conjugate that acts as a glutathione peroxidase mimic.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 11697854
Journal Arch Biochem Biophys
Year 2001
Volume 395
Pages 177-84
Authors Su D, Ren X, You D, Li D, Mu Y, Yan G, Zhang Y, Luo Y, Xue Y, Shen J, Liu Z, Luo G
Title Generation of three selenium-containing catalytic antibodies with high catalytic efficiency using a novel hapten design method.
Related PDB
Related UniProtKB
[16]
Resource
Comments
Medline ID
PubMed ID 11485327
Journal Biochem Biophys Res Commun
Year 2001
Volume 286
Pages 189-94
Authors Su D, You D, Ren X, Luo G, Mu Y, Yan G, Xue Y, Shen J
Title Kinetics study of a selenium-containing ScFv catalytic antibody that mimics glutathione peroxidase.
Related PDB
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID 11456617
Journal J Am Chem Soc
Year 2001
Volume 123
Pages 839-50
Authors Mugesh G, Panda A, Singh HB, Punekar NS, Butcher RJ
Title Glutathione peroxidase-like antioxidant activity of diaryl diselenides: a mechanistic study.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 11678710
Journal Org Lett
Year 2001
Volume 3
Pages 3569-72
Authors Goto K, Nagahama M, Mizushima T, Shimada K, Kawashima T, Okazaki R
Title The first direct oxidative conversion of a selenol to a stable selenenic acid: experimental demonstration of three processes included in the catalytic cycle of glutathione peroxidase.
Related PDB
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID 12371844
Journal J Am Chem Soc
Year 2002
Volume 124
Pages 12104-5
Authors Back TG, Moussa Z
Title Remarkable activity of a novel cyclic seleninate ester as a glutathione peroxidase mimetic and its facile in situ generation from allyl 3-hydroxypropyl selenide.
Related PDB
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 11996219
Journal J Biotechnol
Year 2002
Volume 82
Pages 411-24
Authors Scheller FW, Wollenberger U, Lei C, Jin W, Ge B, Lehmann C, Lisdat F, Fridman V
Title Bioelectrocatalysis by redox enzymes at modified electrodes.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-07-09 2009-02-26