DB code: S00307

CATH domain 3.40.50.300 : Rossmann fold Catalytic domain
E.C. 2.7.4.10
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.40.50.300 : Rossmann fold S00527 S00547 S00548 S00550 S00554 S00555 S00671 S00672 S00676 S00680 S00682 S00913 S00914 S00301 S00302 S00303 S00304 S00308 S00305 S00306 S00309 S00310 S00311 M00114 M00199 D00129 D00130 D00540 M00186

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P08760 GTP:AMP phosphotransferase mitochondrial
EC 2.7.4.10
Adenylate kinase 3
AK 3
Adenylate kinase 3 alpha-like 1
NP_776662.1 (Protein)
NM_174237.2 (DNA/RNA sequence)
PF00406 (ADK)
PF05191 (ADK_lid)
[Graphical View]

KEGG enzyme name
nucleoside-triphosphate---adenylate kinase
guanosine triphosphate-adenylate kinase
nucleoside triphosphate-adenosine monophosphate transphosphorylase
GTP:AMP phosphotransferase
isozyme 3 of adenylate kinase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P08760 KAD3_BOVIN NTP + AMP = NDP + ADP. Monomer. Mitochondrion matrix.

KEGG Pathways
Map code Pathways E.C.
MAP00240 Pyrimidine metabolism

Compound table
Substrates Products Intermediates
KEGG-id C00044 C00020 C00035 C00008
E.C.
Compound GTP AMP GDP ADP
Type amide group,amine group,nucleotide amine group,nucleotide amide group,amine group,nucleotide amine group,nucleotide
ChEBI 15996
16027
17552
16761
PubChem 6830
6083
8977
6022
1ak3A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:AMP Unbound Unbound
1ak3B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:AMP Unbound Unbound
2ak3A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:AMP Unbound Unbound
2ak3B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:AMP Unbound Unbound

Reference for Active-site residues
resource references E.C.
similar to S00305

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1ak3A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 18;ARG 122
1ak3B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 18;ARG 122
2ak3A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 18;ARG 122
2ak3B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain LYS 18;ARG 122

References for Catalytic Mechanism
References Sections No. of steps in catalysis

References
[1]
Resource
Comments
Medline ID
PubMed ID 3013690
Journal FEBS Lett
Year 1986
Volume 202
Pages 303-8
Authors Tomasselli AG, Frank R, Schiltz E
Title The complete primary structure of GTP:AMP phosphotransferase from beef heart mitochondria.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 2175649
Journal Biochemistry
Year 1990
Volume 29
Pages 8138-44
Authors Diederichs K, Schulz GE
Title Three-dimensional structure of the complex between the mitochondrial matrix adenylate kinase and its substrate AMP.
Related PDB 1ak3
Related UniProtKB P08760
[3]
Resource
Comments
Medline ID
PubMed ID 1994037
Journal J Mol Biol
Year 1991
Volume 217
Pages 541-9
Authors Diederichs K, Schulz GE
Title The refined structure of the complex between adenylate kinase from beef heart mitochondrial matrix and its substrate AMP at 1.85 A resolution.
Related PDB 2ak3
Related UniProtKB P08760
[4]
Resource
Comments
Medline ID
PubMed ID 8537371
Journal J Biol Chem
Year 1995
Volume 270
Pages 31103-10
Authors Schricker R, Magdolen V, Strobel G, Bogengruber E, Breitenbach M, Bandlow W
Title Strain-dependent occurrence of functional GTP:AMP phosphotransferase (AK3) in Saccharomyces cerevisiae.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 9665856
Journal J Mol Biol
Year 1998
Volume 280
Pages 551-8
Authors Yamada M, Sugahara M, Hishitani Y, Nobumoto M, Nakazawa A
Title Isolation and characterization of mutated mitochondrial GTP:AMP phosphotransferase.
Related PDB
Related UniProtKB

Comments
This enzyme belongs to the adenylate kinase family.
This enzyme, adenylate kinase isozyme-3 (GTP:AMP phosphotransferase), is related to other adenylate kinases (see S00305).

Created Updated
2002-05-31 2009-02-26