DB code: S00327

RLCP classification 9.5010.536200.8010 : Hydride transfer
9.1050.440000.8010 : Hydride transfer
CATH domain 3.40.50.720 : Rossmann fold Catalytic domain
E.C. 1.1.1.62
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.40.50.720 : Rossmann fold S00543 S00551 S00552 S00553 S00602 S00604 S00605 S00608 S00610 S00625 S00319 S00328 S00329 S00330 S00331 S00332 D00456 D00457 D00458 S00324 S00320 S00325 S00326 D00459 S00335 S00336 S00334 T00219 S00339 D00513 D00001 D00002 D00003 D00005 D00007 D00008 D00010 D00012 D00017 D00018 D00023 D00027 D00028 D00031 D00032 D00033 D00034 D00035 D00037 D00048 D00071 D00476 D00481 D00482 D00490 D00492 D00494 D00545 D00601 D00603 D00604 D00605 D00615 D00845 D00857 D00858 M00161 M00171 M00210 T00002 T00010 T00011 T00015 T00227 T00247 T00408 T00414 D00827 D00262 D00274 D00275 M00035 T00109

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P14061 Estradiol 17-beta-dehydrogenase 1
EC 1.1.1.62
17-beta-hydroxysteroid dehydrogenase type 1
17-beta-HSD 1
Placental 17-beta-hydroxysteroid dehydrogenase
20 alpha-hydroxysteroid dehydrogenase
20-alpha-HSD
E2DH
NP_000404.2 (Protein)
NM_000413.2 (DNA/RNA sequence)
PF00106 (adh_short)
[Graphical View]
Q8NBQ5 Estradiol 17-beta-dehydrogenase 11
EC 1.1.1.62
17-beta-hydroxysteroid dehydrogenase 11
17-beta-HSD 11
17betaHSD11
17bHSD11
17-beta-HSD XI
17betaHSDXI
Dehydrogenase/reductase SDR family member 8
Retinal short-chain dehydrogenase/reductase 2
retSDR2
Cutaneous T-cell lymphoma-associated antigen HD-CL-03
CTCL tumor antigen HD-CL-03
NP_057329.2 (Protein)
NM_016245.3 (DNA/RNA sequence)
PF00106 (adh_short)
[Graphical View]

KEGG enzyme name
estradiol 17beta-dehydrogenase
20alpha-hydroxysteroid dehydrogenase
17beta,20alpha-hydroxysteroid dehydrogenase
17beta-estradiol dehydrogenase
estradiol dehydrogenase
estrogen 17-oxidoreductase
17beta-HSD

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
Q8NBQ5 DHB11_HUMAN Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. Secreted (Potential).
P14061 DHB1_HUMAN Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. Homodimer. Cytoplasm.

KEGG Pathways
Map code Pathways E.C.
MAP00150 Androgen and estrogen metabolism

Compound table
Substrates Products Intermediates
KEGG-id C00003 C00006 C00951 C00004 C00005 C00468 C00080
E.C.
Compound NAD+ NADP+ Estradiol-17beta NADH NADPH Estrone H+
Type amide group,amine group,nucleotide amide group,amine group,nucleotide aromatic ring (only carbon atom),carbohydrate,steroid amide group,amine group,nucleotide amide group,amine group,nucleotide aromatic ring (only carbon atom),carbohydrate,steroid others
ChEBI 15846
18009
16469
16908
16474
17263
15378
PubChem 5893
5886
5757
439153
5884
5870
1038
1a27A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Bound:EST Unbound Unbound Unbound
1bhsA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound
1dhtA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:DHT Unbound Unbound Unbound
1equA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound Unbound Analogue:EQI
1equB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound Unbound Unbound
1fdsA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:EST Unbound Unbound Unbound
1fdtA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Bound:EST Unbound Unbound Unbound
1fduA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Bound:EST Unbound Unbound Unbound
1fduB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Bound:EST Unbound Unbound Unbound
1fduC Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Bound:EST Unbound Unbound Unbound
1fduD Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Bound:EST Unbound Unbound Unbound
1fdvA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound Unbound Unbound Unbound
1fdvB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound Unbound Unbound Unbound
1fdvC Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound Unbound Unbound Unbound
1fdvD Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:NAD Unbound Unbound Unbound Unbound Unbound
1fdwA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:EST Unbound Unbound Unbound
1i5rA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Analogue:HYC(Adenosine of NAD) Unbound Analogue:HYC(Estradiol) Unbound Unbound Unbound
1iolA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Bound:EST Unbound Unbound Unbound
1jtvA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:TES(disoriented) Unbound Unbound Unbound
1qyvA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Unbound Unbound Unbound Unbound
1qywA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:NAP Unbound Unbound Unbound Analogue:5SD(disoriented)
1qyxA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:NAP Unbound Unbound Unbound Analogue:ASD(disoriented)
3deyX Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:DHT(disoriented) Unbound Unbound Unbound
3dheA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Analogue:AND
3hb4X Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:E2B Unbound Unbound Unbound
3hb5X Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Analogue:E2B Unbound Unbound Unbound
3klmX Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:DHT(disoriented) Unbound Unbound Unbound
3klpX Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Analogue:B81(disoriented) Unbound Unbound Unbound
3km0A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Analogue:AOM Unbound Unbound Unbound
3km0B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Bound:NAP Analogue:AOM Unbound Unbound Unbound
1yb1A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Analogue:AE2(disoriented)
1yb1B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Analogue:AE2(disoriented)

Reference for Active-site residues
resource references E.C.
Swiss-prot;P14061 & literature [16], [19] & [20]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1a27A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1bhsA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1dhtA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1equA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1equB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1fdsA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1fdtA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1fduA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159 mutant H221L
1fduB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159 mutant H221L
1fduC Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159 mutant H221L
1fduD Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159 mutant H221L
1fdvA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159 mutant H221L
1fdvB Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159 mutant H221L
1fdvC Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159 mutant H221L
1fdvD Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159 mutant H221L
1fdwA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159 mutant H221Q
1i5rA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1iolA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1jtvA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1qyvA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1qywA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1qyxA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
3deyX Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
3dheA Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
3hb4X Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
3hb5X Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
3klmX Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
3klpX Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
3km0A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
3km0B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 142;TYR 155;LYS 159
1yb1A Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 167;TYR 180;LYS 184
1yb1B Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain SER 167;TYR 180;LYS 184

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[13]
Fig.1, p.1061-1062
[14]
Fig.1, p.200-201
[16]
Fig.7, p.505-508
[18]
Fig.5, p.S19
[19]
p.667-668
[20]
Fig.7, p.910-913
[26]
Fig.2, p.841-842
[28]
p.1109

References
[1]
Resource
Comments
Medline ID
PubMed ID 240714
Journal Eur J Biochem
Year 1975
Volume 56
Pages 557-61
Authors Biellmann JF, Hirth CG
Title NAD(P)+ analogues: tools for the investigation of the active site of oestradiol 17beta-dehydrogenase from human placenta.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 976263
Journal Eur J Biochem
Year 1976
Volume 68
Pages 385-94
Authors Pons M, Nicolas JC, Boussioux AM, Descomps B, Crastes de Paulet A
Title Affinity labelling of the estradiol-17 beta dehydrogenase from human placenta with substrate analogs.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 570483
Journal Endocrinology
Year 1978
Volume 102
Pages 1398-1403
Authors Tseng L
Title Steroid specificity in the stimulation of human endometrial estradiol dehydrogenase.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 6949898
Journal J Biol Chem
Year 1982
Volume 257
Pages 2225-9
Authors Chin CC, Pineda J, Warren JC
Title Spatial relationship of steroid and cofactor at the active site of human placental estradiol 17 beta-dehydrogenase.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 6949900
Journal J Biol Chem
Year 1982
Volume 257
Pages 2783-6
Authors Tobias B, Covey DF, Strickler RC
Title Inactivation of human placental 17 beta-estradiol dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase with active site-directed 17 beta-propynyl-substituted progestin analogs.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 6572146
Journal Eur J Biochem
Year 1983
Volume 129
Pages 691-5
Authors Inano H, Tamaoki B
Title Affinity labeling of arginyl residues at the catalytic region of estradiol 17 beta-dehydrogenase from human placenta by 16-oxoestrone.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 6580513
Journal J Steroid Biochem
Year 1983
Volume 19
Pages 1617-22
Authors Inano H, Ohba H, Tamaoki B
Title Photochemical inactivation of human placental estradiol 17 beta-dehydrogenase in the presence of 2,3-butanedione.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 3456799
Journal Biochemistry
Year 1986
Volume 25
Pages 641-6
Authors Murdock GL, Chin CC, Warren JC
Title Human placental estradiol 17 beta-dehydrogenase: sequence of a histidine-bearing peptide in the catalytic region.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 3130054
Journal Biochem Biophys Res Commun
Year 1988
Volume 152
Pages 789-93
Authors Inano H
Title Chemical modification of lysine residues at active-site of human placental estradiol 17 beta-dehydrogenase.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 2846351
Journal FEBS Lett
Year 1988
Volume 239
Pages 73-7
Authors Peltoketo H, Isomaa V, Maentausta O, Vihko R
Title Complete amino acid sequence of human placental 17 beta-hydroxysteroid dehydrogenase deduced from cDNA.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 2772972
Journal Steroids
Year 1989
Volume 53
Pages 77-96
Authors Auchus RJ, Palmer JO, Carrell HL, Covey DF
Title Preparation of 14,15-secoestra-1,3,5(10)-trien-15-ynes, inhibitors of estradiol dehydrogenase.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 2146972
Journal J Steroid Biochem Mol Biol
Year 1990
Volume 37
Pages 65-70
Authors Pineda JA, Murdock GL, Watson RJ, Warren JC
Title Stereospecificity of hydrogen transfer between progesterone and cofactor by human placental estradiol-17 beta dehydrogenase.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 1953709
Journal Biochem Biophys Res Commun
Year 1991
Volume 180
Pages 1057-63
Authors Sweet F, Boyd J, Medina O, Konderski L, Murdock GL
Title Hydrogen bonding in steroidogenesis: studies on new heterocyclic analogs of estrone that inhibit human estradiol 17 beta-dehydrogenase.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 1998720
Journal Biochim Biophys Acta
Year 1991
Volume 1076
Pages 197-202
Authors Murdock GL, Pineda J, Nagorsky N, Lawrence SS, Heritage R, Warren JC
Title Estradiol 17 beta-dehydrogenase: full enzymatic activity in the absence of zinc.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 8200347
Journal Eur J Biochem
Year 1994
Volume 222
Pages 221-7
Authors Leenders F, Adamski J, Husen B, Thole HH, Jungblut PW
Title Molecular cloning and amino acid sequence of the porcine 17 beta-estradiol dehydrogenase.
Related PDB
Related UniProtKB
[16]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.2 AND 1.7 ANGSTROMS).
Medline ID 95393021
PubMed ID 7663947
Journal Structure
Year 1995
Volume 3
Pages 503-13
Authors Ghosh D, Pletnev VZ, Zhu DW, Wawrzak Z, Duax WL, Pangborn W, Labrie F, Lin SX
Title Structure of human estrogenic 17 beta-hydroxysteroid dehydrogenase at 2.20 A resolution.
Related PDB 1bhs
Related UniProtKB P14061
[17]
Resource
Comments
Medline ID
PubMed ID 8917625
Journal Biochim Biophys Acta
Year 1996
Volume 1297
Pages 219-27
Authors Mendoza-Hernandez G, Rendon JL
Title Human placental estradiol 17 beta-dehydrogenase: structural and catalytic changes during urea denaturation.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 8943782
Journal J Endocrinol
Year 1996
Volume 150 Suppl
Pages S13-20
Authors Lin SX, Zhu DW, Azzi A, Campbell RL, Breton R, Labrie F, Ghosh D, Pletnev V, Duax WL, Pangborn W
Title Studies on the three-dimensional structure of estrogenic 17 beta-hydroxysteroid dehydrogenase.
Related PDB
Related UniProtKB
[19]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8756321
Journal Nat Struct Biol
Year 1996
Volume 3
Pages 665-8
Authors Azzi A, Rehse PH, Zhu DW, Campbell RL, Labrie F, Lin SX
Title Crystal structure of human estrogenic 17 beta-hydroxysteroid dehydrogenase complexed with 17 beta-estradiol.
Related PDB 1iol
Related UniProtKB
[20]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
Medline ID 96398680
PubMed ID 8805577
Journal Structure
Year 1996
Volume 4
Pages 905-15
Authors Breton R, Housset D, Mazza C, Fontecilla-Camps JC
Title The structure of a complex of human 17beta-hydroxysteroid dehydrogenase with estradiol and NADP+ identifies two principal targets for the design of inhibitors.
Related PDB 1fds 1fdt
Related UniProtKB P14061
[21]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID
Journal Grenoble : Universite Joseph Fourier (Thesis)
Year 1997
Volume
Pages
Authors Mazza, C
Title Human Type I 17Beta-Hydroxysteroid Dehydrogenase: Site Directed Mutagenesis and X-Ray Crystallography Structure-Function Analysis.
Related PDB 1a27
Related UniProtKB
[22]
Resource
Comments
Medline ID
PubMed ID 8994190
Journal Mol Endocrinol
Year 1997
Volume 11
Pages 77-86
Authors Puranen T, Poutanen M, Ghosh D, Vihko P, Vihko R
Title Characterization of structural and functional properties of human 17 beta-hydroxysteroid dehydrogenase type 1 using recombinant enzymes and site-directed mutagenesis.
Related PDB
Related UniProtKB
[23]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
Medline ID 98192605
PubMed ID 9525918
Journal J Biol Chem
Year 1998
Volume 273
Pages 8145-52
Authors Mazza C, Breton R, Housset D, Fontecilla-Camps JC
Title Unusual charge stabilization of NADP+ in 17beta-hydroxysteroid dehydrogenase.
Related PDB 1fdu 1fdv 1fdw
Related UniProtKB P14061
[24]
Resource
Comments
Medline ID
PubMed ID 10419021
Journal J Steroid Biochem Mol Biol
Year 1999
Volume 69
Pages 425-9
Authors Lin SX, Han Q, Azzi A, Zhu DW, Gangloff A, Campbell RL, Gongloff A
Title 3D-structure of human estrogenic 17beta-HSD1: binding with various steroids.
Related PDB
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID 10622412
Journal J Steroid Biochem Mol Biol
Year 1999
Volume 70
Pages 229-35
Authors Zhu DW, Campbell R, Labrie F, Lin SX
Title Crystallization and preliminary crystal structure of the complex of 17beta-hydroxysteroid dehydrogenase with a dual-site inhibitor.
Related PDB
Related UniProtKB
[26]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9927655
Journal Proc Natl Acad Sci U S A
Year 1999
Volume 96
Pages 840-5
Authors Sawicki MW, Erman M, Puranen T, Vihko P, Ghosh D
Title Structure of the ternary complex of human 17beta-hydroxysteroid dehydrogenase type 1 with 3-hydroxyestra-1,3,5,7-tetraen-17-one (equilin) and NADP+.
Related PDB 1equ
Related UniProtKB
[27]
Resource
Comments
Medline ID
PubMed ID 11368312
Journal Arch Biochem Biophys
Year 2000
Volume 384
Pages 255-62
Authors Rizner TL, Adamski J, Stojan J
Title 17Beta-hydroxysteroid dehydrogenase from Cochliobolus lunatus: model structure and substrate specificity.
Related PDB
Related UniProtKB
[28]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.24 ANGSTROMS) OF 38-327, AND MUTAGENESIS OF LEU-149.
Medline ID
PubMed ID 10625652
Journal J Biol Chem
Year 2000
Volume 275
Pages 1105-11
Authors Han Q, Campbell RL, Gangloff A, Huang YW, Lin SX
Title Dehydroepiandrosterone and dihydrotestosterone recognition by human estrogenic 17beta-hydroxysteroid dehydrogenase. C-18/c-19 steroid discrimination and enzyme-induced strain.
Related PDB 1dht 3dhe
Related UniProtKB
[29]
Resource
Comments
Medline ID
PubMed ID 11719564
Journal Mol Biol Evol
Year 2001
Volume 18
Pages 2154-61
Authors Breitling R, Laubner D, Adamski J
Title Structure-based phylogenetic analysis of short-chain alcohol dehydrogenases and reclassification of the 17beta-hydroxysteroid dehydrogenase family.
Related PDB
Related UniProtKB
[30]
Resource
Comments
Medline ID
PubMed ID 11682630
Journal Mol Endocrinol
Year 2001
Volume 15
Pages 2010-20
Authors Huang YW, Pineau I, Chang HJ, Azzi A, Bellemare V, Laberge S, Lin SX
Title Critical residues for the specificity of cofactors and substrates in human estrogenic 17beta-hydroxysteroid dehydrogenase 1: variants designed from the three-dimensional structure of the enzyme.
Related PDB
Related UniProtKB
[31]
Resource
Comments
Medline ID
PubMed ID 12475215
Journal Biochemistry
Year 2002
Volume 41
Pages 14659-68
Authors Benach J, Filling C, Oppermann UC, Roversi P, Bricogne G, Berndt KD, Jornvall H, Ladenstein R
Title Structure of bacterial 3beta/17beta-hydroxysteroid dehydrogenase at 1.2 A resolution: a model for multiple steroid recognition.
Related PDB
Related UniProtKB
[32]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12223444
Journal FASEB J
Year 2002
Volume 16
Pages 1829-31
Authors Qiu W, Campbell RL, Gangloff A, Dupuis P, Boivin RP, Tremblay MR, Poirier D, Lin SX
Title A concerted, rational design of type 1 17beta-hydroxysteroid dehydrogenase inhibitors: estradiol-adenosine hybrids with high affinity.
Related PDB 1i5r
Related UniProtKB
[33]
Resource
Comments
Medline ID
PubMed ID 12604234
Journal Chem Biol Interact
Year 2003
Volume 143-144
Pages 481-91
Authors Brown WM, Metzger LE, Barlow JP, Hunsaker LA, Deck LM, Royer RE, Vander Jagt DL
Title 17-beta-Hydroxysteroid dehydrogenase type 1: computational design of active site inhibitors targeted to the Rossmann fold.
Related PDB
Related UniProtKB
[34]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 12490543
Journal FASEB J
Year 2003
Volume 17
Pages 274-6
Authors Gangloff A, Shi R, Nahoum V, Lin SX
Title Pseudo-symmetry of C19 steroids, alternative binding orientations, and multispecificity in human estrogenic 17beta-hydroxysteroid dehydrogenase.
Related PDB 1jtv
Related UniProtKB
[35]
Resource
Comments
Medline ID
PubMed ID 15110763
Journal Biochem Biophys Res Commun
Year 2004
Volume 318
Pages 131-4
Authors Owen CP, Ahmed S
Title The derivation of a potential transition state for the reduction reaction catalysed by 17beta-hydroxysteroid dehydrogenase--an approximate representation of its active site for use in drug design and discovery.
Related PDB
Related UniProtKB
[36]
Resource
Comments
Medline ID
PubMed ID 14966133
Journal J Biol Chem
Year 2004
Volume 279
Pages 16778-85
Authors Shi R, Lin SX
Title Cofactor hydrogen bonding onto the protein main chain is conserved in the short chain dehydrogenase/reductase family and contributes to nicotinamide orientation.
Related PDB 1qyv 1qyw 1qyx
Related UniProtKB
[37]
Resource
Comments
Medline ID
PubMed ID 16414178
Journal Mol Cell Endocrinol
Year 2006
Volume 248
Pages 61-71
Authors Lukacik P, Kavanagh KL, Oppermann U
Title Structure and function of human 17beta-hydroxysteroid dehydrogenases.
Related PDB 1yb1
Related UniProtKB
[38]
Resource
Comments
Medline ID
PubMed ID 19929851
Journal Biochem J
Year 2009
Volume 424
Pages 357-66
Authors Mazumdar M, Fournier D, Zhu DW, Cadot C, Poirier D, Lin SX
Title Binary and ternary crystal structure analyses of a novel inhibitor with 17beta-HSD type 1: a lead compound for breast cancer therapy.
Related PDB 3hb4 3hb5
Related UniProtKB
[39]
Resource
Comments
Medline ID
PubMed ID 20172961
Journal Mol Endocrinol
Year 2010
Volume 24
Pages 832-45
Authors Aka JA, Mazumdar M, Chen CQ, Poirier D, Lin SX
Title 17beta-hydroxysteroid dehydrogenase type 1 stimulates breast cancer by dihydrotestosterone inactivation in addition to estradiol production.
Related PDB 3dey 3klm
Related UniProtKB

Comments
This enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily, along with Drosophia alcohol dehydrogenase (S00319 in EzCatDB). This enzyme has got a catalytic triad composed of conserved residues, Ser, Tyr, and Lys. The conformation of these residues, compared to that of the NAD molecule, seems to be similar to that of the homologous enzymes. Thus, its catalytic mechanism must be similar to those of the homologous enzymes.
According to the literature [37], type 1 of this enzyme (17-beta-hydroxysteroid dehydrogenase) (Swiss-prot;P14061) catalyzes the reduction of estrone to estradiol-17-beta, whereas type 11 (Swiss-prot;Q8NBQ5) catalyzes the oxidation/dehydrogenation of 5-alpha-androstanediol to androstrone.

Created Updated
2004-07-13 2011-06-24