DB code: T00082

CATH domain 3.40.50.20 : Rossmann fold
3.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1 Catalytic domain
3.30.1490.20 : Dna Ligase; domain 1
E.C. 4.1.1.21
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
3.30.1490.20 : Dna Ligase; domain 1 M00035 M00037 T00107 T00108
3.30.470.20 : D-amino Acid Aminotransferase; Chain A, domain 1 D00298 M00035 M00037 M00051 T00107 T00108
3.40.50.20 : Rossmann fold M00037 T00107 T00108

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P09029 N5-carboxyaminoimidazole ribonucleotide synthase
N5-CAIR synthase
EC 6.3.4.18
5-(carboxyamino)imidazole ribonucleotide synthetase
NP_415055.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_488811.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF02222 (ATP-grasp)
[Graphical View]

KEGG enzyme name
phosphoribosylaminoimidazole carboxylase
5-phosphoribosyl-5-aminoimidazole carboxylase
5-amino-1-ribosylimidazole 5-phosphate carboxylase
AIR carboxylase
1-(5-phosphoribosyl)-5-amino-4-imidazolecarboxylate carboxy-lyase
ADE2
class II PurE
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate carboxy-lyase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P09029 PURK_ECOLI 5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2). Homodimer.

KEGG Pathways
Map code Pathways E.C.
MAP00230 Purine metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00305 C03373 C00011 C00002 C00001 L00008 C00008 C00009
E.C.
Compound Magnesium 1-(5-Phospho-D-ribosyl)-5-aminoimidazole CO2 ATP H2O 1-(5-Phospho-D-ribosyl)-5-carboxyamino-imidazole ADP Orthophosphate
Type divalent metal (Ca2+, Mg2+) amine group,nucleotide others amine group,nucleotide H2O amide group,carboxyl group,nucleotide amine group,nucleotide phosphate group/phosphate ion
ChEBI 18420
16526
15422
15377
48000
16761
26078
PubChem 888
161500
280
5957
22247451
962
23657851
6022
1004
22486802
1b6rA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b6sA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b6sB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b6sC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b6sD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b6rA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Analogue:SO4
1b6sA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Unbound Unbound Unbound Unbound Bound:ADP Unbound
1b6sB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Unbound Unbound Unbound Unbound Bound:ADP Unbound
1b6sC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Unbound Unbound Unbound Unbound Bound:ADP Unbound
1b6sD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MG Unbound Unbound Unbound Unbound Bound:ADP Unbound
1b6rA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b6sA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b6sB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b6sC03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound
1b6sD03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
PDB;1b6s

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1b6rA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1b6sA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1b6sB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1b6sC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1b6sD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1b6rA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 226;GLU 238(magnesium binding)
1b6sA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 226;GLU 238(magnesium binding)
1b6sB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 226;GLU 238(magnesium binding)
1b6sC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 226;GLU 238(magnesium binding)
1b6sD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 226;GLU 238(magnesium binding)
1b6rA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1b6sA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1b6sB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1b6sC03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1b6sD03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[4]
p.15489, p.15491

References
[1]
Resource
Comments CHARACTERIZATION, AND SEQUENCE OF 1-20.
Medline ID 92287929
PubMed ID 1534690
Journal Biochemistry
Year 1992
Volume 31
Pages 5022-32
Authors Meyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM
Title Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.
Related PDB
Related UniProtKB P09029
[2]
Resource
Comments
Medline ID
PubMed ID 7918411
Journal Biochemistry
Year 1994
Volume 33
Pages 11927-34
Authors Firestine SM, Poon SW, Mueller EJ, Stubbe J, Davisson VJ
Title Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 10074353
Journal Biochemistry
Year 1999
Volume 38
Pages 3012-8
Authors Meyer E, Kappock TJ, Osuji C, Stubbe J
Title Evidence for the direct transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia coli PurE, an N5-CAIR mutase.
Related PDB
Related UniProtKB
[4]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
Medline ID 20039871
PubMed ID 10569930
Journal Biochemistry
Year 1999
Volume 38
Pages 15480-92
Authors Thoden JB, Kappock TJ, Stubbe J, Holden HM
Title Three-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily.
Related PDB 1b6r 1b6s
Related UniProtKB P09029

Comments

Created Updated
2004-03-25 2009-02-26