DB code: T00100

CATH domain 3.40.50.1070 : Rossmann fold
3.40.275.10 : L-fucose Isomerase; Chain A, domain 2 Catalytic domain
3.20.14.10 : L-fucose Isomerase; Chain A, domain 3 Catalytic domain
E.C. 5.3.1.25
CSA 1fui
M-CSA 1fui
MACiE M0095

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P69922 L-fucose isomerase
EC 5.3.1.25
6-deoxy-L-galactose isomerase
FucIase
NP_417282.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491010.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF02952 (Fucose_iso_C)
PF07881 (Fucose_iso_N1)
PF07882 (Fucose_iso_N2)
[Graphical View]

KEGG enzyme name
L-fucose isomerase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P69922 FUCI_ECOLI L-fucose = L-fuculose. Homohexamer. Cytoplasm. Manganese.

KEGG Pathways
Map code Pathways E.C.
MAP00051 Fructose and mannose metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00034 C01019 C01721
E.C.
Compound Manganese L-Fucose L-Fuculose
Type heavy metal carbohydrate carbohydrate
ChEBI 18291
35154
2181
17617
PubChem 23930
17106
6857362
1fuiA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Unbound Unbound
1fuiA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Analogue:FOC Unbound
1fuiB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Analogue:FOC Unbound
1fuiC03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Analogue:FOC Unbound
1fuiD03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Analogue:FOC Unbound
1fuiE03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Bound:_MN Analogue:FOC Unbound
1fuiF03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain Unbound Analogue:FOC Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P69922 & literature [7]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1fuiA01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1fuiB01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1fuiC01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1fuiD01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1fuiE01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1fuiF01 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain
1fuiA02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 337 GLU 337(Manganese binding)
1fuiB02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 337 GLU 337(Manganese binding)
1fuiC02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 337 GLU 337(Manganese binding)
1fuiD02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 337 GLU 337(Manganese binding)
1fuiE02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 337 GLU 337(Manganese binding)
1fuiF02 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain GLU 337 GLU 337(Manganese binding)
1fuiA03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 361 ASP 361;HIS 528(Manganese binding)
1fuiB03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 361 ASP 361;HIS 528(Manganese binding)
1fuiC03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 361 ASP 361;HIS 528(Manganese binding)
1fuiD03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 361 ASP 361;HIS 528(Manganese binding)
1fuiE03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 361 ASP 361;HIS 528(Manganese binding)
1fuiF03 Pdbj logo s Rasmollogo id Rasmollogo chain Mmcif id Mmcif chain ASP 361 ASP 361;HIS 528(Manganese binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[7]
Fig.11, p.260-262, p.264-266
[10]
Scheme 16, p.9-10
[11]
p.101

References
[1]
Resource
Comments
Medline ID
PubMed ID 13319278
Journal J Biol Chem
Year 1956
Volume 219
Pages 557-68
Authors GREEN M, COHEN SS
Title Enzymatic conversion of L-fucose to L-fuculose.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID
Journal Methods Enzymol
Year 1966
Volume 9
Pages 583-5
Authors Mortlock RP
Title D-Arabinose isomerase.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 4632320
Journal J Bacteriol
Year 1973
Volume 113
Pages 687-96
Authors Boulter JR, Gielow WO
Title Properties of D-arabinose isomerase purified from two strains of Escherichia coli.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID
Journal Agric Biol Chem
Year 1974
Volume 38
Pages 267-73
Authors Izumori K, Yamanaka K
Title Purification and crystallization of D-arabinose (L-fucose) isomerase from Aerobacter aerogenes by polyethylene glycol.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID
Journal Agric Biol Chem
Year 1976
Volume 40
Pages 439-440
Authors Yamanaka K, Izumori K
Title Inhibition of D-arabinose (L-fucose) isomerase activity by Tris and its analogues.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID
Journal Agric Biol Chem
Year 1979
Volume 43
Pages 1993-4
Authors Izumori K, Yamanaka K
Title Activation and inhibition of D-arabinose isomerase of Klebsiella aerogenes by L-histidine.
Related PDB
Related UniProtKB
[7]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
Medline ID 98035055
PubMed ID 9367760
Journal J Mol Biol
Year 1997
Volume 273
Pages 256-68
Authors Seemann JE, Schulz GE
Title Structure and mechanism of L-fucose isomerase from Escherichia coli.
Related PDB 1fui
Related UniProtKB P69922
[8]
Resource
Comments
Medline ID
PubMed ID 9485504
Journal Proteins
Year 1997
Volume Suppl 1
Pages 129-33
Authors Flockner H, Domingues FS, Sippl MJ
Title Protein folds from pair interactions: a blind test in fold recognition.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 10368289
Journal Structure Fold Des
Year 1999
Volume 7
Pages 227-36
Authors Castillo RM, Mizuguchi K, Dhanaraj V, Albert A, Blundell TL, Murzin AG
Title A six-stranded double-psi beta barrel is shared by several protein superfamilies.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 11368170
Journal Arch Biochem Biophys
Year 2001
Volume 387
Pages 1-10
Authors Creighton DJ, Hamilton DS
Title Brief history of glyoxalase I and what we have learned about metal ion-dependent, enzyme-catalyzed isomerizations.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID
Journal Top Curr Chem
Year 2001
Volume 215
Pages 77-114
Authors Hausler H, Stutz AE
Title D-xylose (D-glucose) isomerase and related enzymes in carbohydrate synthesis.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 12645017
Journal Chemistry
Year 2003
Volume 9
Pages 1281-95
Authors Casanova D, Alemany P, Bofill JM, Alvarez S
Title Shape and symmetry of heptacoordinate transition-metal complexes: structural trends.
Related PDB
Related UniProtKB

Comments

Created Updated
2005-07-04 2009-02-26