List of catalytic reactions

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References:
Nagano et al., (2007) PROTEINS, Structure, Function and Bioinformatics, 66, 147-159.


RLCP codes/
links
Overall reaction Ligand
types
Catalysis
types
Types of
catalytic sites
Corresponding enzymes
1.12.30000.10Hydrolysis Carboxylic Ester (One of Carbonyl Ester bonds)Trypsin-like mechanismSer/His/Asp + mainchain amide
  • S00345; Carboxylesterase 2
    (Catalytic domain: 3.40.50.1820)
  • S00347; Bile salt-activated lipase
    (Catalytic domain: 3.40.50.1820)
  • S00720; Arylesterase
    (Catalytic domain: 3.40.50.1820)
  • S00723; Feruloyl esterase A
    (Catalytic domain: 3.40.50.1820)
  • T00253; Pancreatic lipase-related protein 2
    (Catalytic domain: 3.40.50.1820)
  • 1.12.30000.14Hydrolysis Carboxylic Ester (One of Carbonyl Ester bonds)Trypsin-like mechanismSer/His/Glu + mainchain amide
  • S00057; Acetylcholinesterase
    (Catalytic domain: 3.40.50.1820)
  • S00725; Cholinesterase
    (Catalytic domain: 3.40.50.1820)
  • 1.12.30000.24Hydrolysis Carboxylic Ester (One of Carbonyl Ester bonds)Trypsin-like mechanismSer/His/Asp + Asn/mainchain amide
  • S00361; Platelet-activating factor acetylhydrolase IB subunit gamma
    (Catalytic domain: 3.40.50.1110)
  • 1.12.30000.26Hydrolysis Carboxylic Ester (One of Carbonyl Ester bonds)Trypsin-like mechanismSer/His/Asp + Thr/mainchain amide
  • S00346; Acetylxylan esterase 2
    (Catalytic domain: 3.40.50.1820)
  • 1.12.30000.27Hydrolysis Carboxylic Ester (One of Carbonyl Ester bonds)Trypsin-like mechanismSer/His/Asp + Ser/mainchain amide
  • S00724; Cutinase 1
    (Catalytic domain: 3.40.50.1820)
  • 1.12.30000.45Hydrolysis Carboxylic Ester (One of Carbonyl Ester bonds)Trypsin-like mechanismCys/His/Asp + Gln/mainchain amide
  • S00524; Ubiquitin carboxyl-terminal hydrolase YUH1
    (Catalytic domain: 3.40.532.10)
  • 1.12.30190.70Hydrolysis Carboxylic Ester (One of Carbonyl Ester bonds)Pectinesterase A-like mechanismAsp/Asp/Arg + Gln cluster
  • S00171; Pectinesterase A
    (Catalytic domain: 2.160.20.10)
  • 1.12.38700.511Hydrolysis Carboxylic Ester (One of Carbonyl Ester bonds)Phospholipase-A1-like mechanismSer/His/Asn + mainchain amide + calcium ion bound to mainchain carbonyl group and Ser
  • S00127; Phospholipase A1
    (Catalytic domain: 2.40.230.10)
  • 1.13.200.966Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Pepsin-like mechanismTwo acidic residues (interacting through a water)
  • D00231; Saccharopepsin
    (Catalytic domain: 2.40.70.10)
  • D00423; Chymosin
    (Catalytic domain: 2.40.70.10)
  • D00436; Pepsin A
    (Catalytic domain: 2.40.70.10)
  • D00437; Gastricsin
    (Catalytic domain: 2.40.70.10)
  • D00438; Renin-2
    (Catalytic domain: 2.40.70.10)
  • D00439; Penicillopepsin
    (Catalytic domain: 2.40.70.10)
  • D00440; Rhizopuspepsin
    (Catalytic domain: 2.40.70.10)
  • D00441; Endothiapepsin
    (Catalytic domain: 2.40.70.10)
  • D00442; Mucorpepsin
    (Catalytic domain: 2.40.70.10)
  • D00443; Candidapepsin
    (Catalytic domain: 2.40.70.10)
  • D00444; Plasmepsin-2
    (Catalytic domain: 2.40.70.10)
  • D00445; Phytepsin
    (Catalytic domain: 2.40.70.10)
  • D00462; Peptide-N(4)-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F
    (Catalytic domain: 2.60.120.230)
  • D00471; Gag-Pol polyprotein
    (Catalytic domain: 2.40.70.10)
  • D00484; Cathepsin D
    (Catalytic domain: 2.40.70.10)
  • D00529; Pepsin-2B
    (Catalytic domain: 2.40.70.10)
  • M00166; Gag-Pol polyprotein
    (Catalytic domains: 2.40.70.10, 3.30.70.270, 3.30.420.10)
  • M00206; Pol polyprotein
    (Catalytic domains: 2.40.70.10, 2.70.40.10)
  • 1.13.7495.453Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)E.coli N-Acetyl-D-glucosamine-6-phosphate deacetylase-like mechanismAn acidic residue + His + zinc ion bound to His/(acidic residue) cluster
  • D00801; N-acetylglucosamine-6-phosphate deacetylase
    (Catalytic domain: 3.20.20.140)
  • 1.13.7500.450Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)One zinc beta-lactamase mechanismAn acidic residue + [Asn or Gln] + zinc ion bound to His/Cys/Asp cluster
  • S00432; Beta-lactamase 2
    (Catalytic domain: 3.60.15.10)
  • 1.13.7630.541Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)N-Acetylglucosamine-6-phosphate deacetylase-like mechanismAn acidic residue + Two Fe ions bound to His/Asp/Glu cluster
  • D00673; N-acetylglucosamine-6-phosphate deacetylase
    (Catalytic domain: 3.20.20.140)
  • 1.13.10000.1220Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Two zinc beta-lactamase mechanismAsn + two zinc ions bound to His/Cys/Asp cluster
  • S00515; Beta-lactamase type II
    (Catalytic domain: 3.60.15.10)
  • 1.13.10880.280Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Muramoyl-pentapeptide-carboxypeptidase-like mechanismHis/Asp + His/Tyr + zinc ion bound to His/His/Asp
  • D00191; Zinc D-Ala-D-Ala carboxypeptidase
    (Catalytic domain: 3.30.1380.10)
  • 1.13.10900.463Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Thermolysin-like mechanismGlu + His/Tyr/Asn + mainchain carbonyl + zinc ion bound to His/Glu cluster
  • D00233; Pseudolysin
    (Catalytic domains: 1.10.390.10, 3.10.170.10)
  • D00234; Thermolysin
    (Catalytic domains: 1.10.390.10, 3.10.170.10)
  • D00235; Bacillolysin
    (Catalytic domains: 1.10.390.10, 3.10.170.10)
  • D00480; Zinc metalloproteinase aureolysin
    (Catalytic domains: 1.10.390.10, 3.10.170.10)
  • 1.13.10900.462Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Thermolysin-like mechanismGlu + Asn + mainchain carbonyl + zinc ion bound to His cluster
  • D00232; Interstitial collagenase
    (Catalytic domain: 3.40.390.10)
  • 1.13.11100.261Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Serralysin-like mechanismGlu/Tyr + zinc ion bound to His cluster
  • D00236; Serralysin
    (Catalytic domain: 3.40.390.10)
  • S00394; Astacin
    (Catalytic domain: 3.40.390.10)
  • 1.13.11100.285Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Serralysin-like mechanismTyr/Lys + zinc ion bound to His/His/Cys
  • S00502; N-acetylmuramoyl-L-alanine amidase
    (Catalytic domain: 3.40.80.10)
  • 1.13.11100.286Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Serralysin-like mechanismTyr/His + zinc ion bound to His/His/Cys
  • S00536; Peptidoglycan-recognition protein-LB
    (Catalytic domain: 3.40.80.10)
  • 1.13.11100.561Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Serralysin-like mechanismGlu/Gln + mainchain amide + Fe(II) bound to Cys/His/His
  • S00442; Peptide deformylase
    (Catalytic domain: 3.90.45.10)
  • 1.13.11110.262Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Carboxypeptidase A-like mechanismAn acidic residue + Arg + mainchain carbonyl + zinc ion bound to His/Glu cluster
  • D00193; Carboxypeptidase A2
    (Catalytic domain: 3.40.630.10)
  • D00467; Carboxypeptidase A
    (Catalytic domain: 3.40.630.10)
  • D00512; Carboxypeptidase B
    (Catalytic domain: 3.40.630.10)
  • S00407; Carboxypeptidase T
    (Catalytic domain: 3.40.630.10)
  • 1.13.11400.460Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Adamalysin-II-like mechanismGlu + zinc ion bound to His cluster
  • M00029; Leishmanolysin
    (Catalytic domains: 3.10.170.20, 3.90.132.10)
  • S00398; Zinc metalloproteinase atrolysin-D
    (Catalytic domain: 3.40.390.10)
  • S00399; Zinc metalloproteinase adamalysin-2
    (Catalytic domain: 3.40.390.10)
  • 1.13.11400.1261Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Adamalysin-II-like mechanismGlu + two zinc ions bound to His/Asp cluster
  • D00192; Carboxypeptidase G2 (CPDG2) (EC 3.4.17.11) (Folate hydrolase G2) (Glutamate carboxypeptidase) (Pteroylmonoglutamic acid hydrolase G2)AltName: INN=Glucarpidase;
    (Catalytic domain: 3.40.630.10)
  • S00406; Bacterial leucyl aminopeptidase
    (Catalytic domain: 3.40.630.10)
  • 1.13.13000.460Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Matrilysin-like mechanismGlu + zinc ion bound to His cluster
  • S00395; Matrilysin
    (Catalytic domain: 3.40.390.10)
  • S00397; Neutrophil collagenase
    (Catalytic domain: 3.40.390.10)
  • 1.13.30000.9Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismSer/His/Asp + Asn/Thr/mainchain amide
  • D00219; Subtilisin Carlsberg
    (Catalytic domain: 3.40.50.200)
  • S00295; Proteinase K
    (Catalytic domain: 3.40.50.200)
  • S00519; Thermitase
    (Catalytic domain: 3.40.50.200)
  • 1.13.30000.10Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismSer/His/Asp + mainchain amide
  • D00189; Carboxypeptidase Y
    (Catalytic domain: 3.40.50.1820)
  • D00194; Chymotrypsin-1
    (Catalytic domain: 2.40.10.10)
  • D00197; Trypsin
    (Catalytic domain: 2.40.10.10)
  • D00211; Brachyurin
    (Catalytic domain: 2.40.10.10)
  • D00212; Glandular kallikrein
    (Catalytic domain: 2.40.10.10)
  • D00214; Elastase-1
    (Catalytic domain: 2.40.10.10)
  • D00216; Neutrophil elastase
    (Catalytic domain: 2.40.10.10)
  • D00224; Streptogrisin-B
    (Catalytic domain: 2.40.10.10)
  • D00426; Cathepsin G
    (Catalytic domain: 2.40.10.10)
  • D00428; Chymase
    (Catalytic domain: 2.40.10.10)
  • D00429; Complement factor D
    (Catalytic domain: 2.40.10.10)
  • D00430; Protease 1
    (Catalytic domain: 2.40.10.10)
  • D00431; Tryptase beta-2
    (Catalytic domain: 2.40.10.10)
  • D00432; Elastase-2A
    (Catalytic domain: 2.40.10.10)
  • D00433; Myeloblastin
    (Catalytic domain: 2.40.10.10)
  • D00434; Streptogrisin-A
    (Catalytic domain: 2.40.10.10)
  • D00435; Glutamyl endopeptidase 2
    (Catalytic domain: 2.40.10.10)
  • D00497; Tonin
    (Catalytic domain: 2.40.10.10)
  • D00528; Epidermal growth factor-binding protein type B
    (Catalytic domain: 2.40.10.10)
  • D00848; Snake venom serine protease Dav-PA
    (Catalytic domain: 2.40.10.10)
  • D00850; Kallikrein-8
    (Catalytic domain: 2.40.10.10)
  • D00851; Glutamyl endopeptidase
    (Catalytic domain: 2.40.10.10)
  • D00852; Granzyme A
    (Catalytic domain: 2.40.10.10)
  • D00855; Granzyme B
    (Catalytic domain: 2.40.10.10)
  • M00122; ATP-dependent Clp protease proteolytic subunit
    (Catalytic domain: 3.90.226.10)
  • M00133; Coagulation factor IX
    (Catalytic domain: 2.40.10.10)
  • M00139; Complement C1r subcomponent
    (Catalytic domain: 2.40.10.10)
  • M00152; Urokinase-type plasminogen activator
    (Catalytic domain: 2.40.10.10)
  • M00155; Tissue-type plasminogen activator (t-PA) (t-plasminogen activator) (tPA) (EC 3.4.21.68)AltName: INN=Alteplase;AltName: INN=Reteplase;
    (Catalytic domain: 2.40.10.10)
  • M00167; Alpha-lytic protease
    (Catalytic domain: 2.40.10.10)
  • M00181; Kallikrein 1-related peptidase b3
    (Catalytic domain: 2.40.10.10)
  • M00212; Vitamin K-dependent protein C
    (Catalytic domain: 2.40.10.10)
  • M00227; Enteropeptidase
    (Catalytic domain: 2.40.10.10)
  • M00315; Mannan-binding lectin serine protease 2
    (Catalytic domain: 2.40.10.10)
  • M00316; Complement C1s subcomponent
    (Catalytic domain: 2.40.10.10)
  • M00317; Complement factor B
    (Catalytic domain: 2.40.10.10)
  • M00348; Suppressor of tumorigenicity 14 protein
    (Catalytic domain: 2.40.10.10)
  • M00349; Serine protease hepsin
    (Catalytic domain: 2.40.10.10)
  • S00353; Proline iminopeptidase
    (Catalytic domain: 3.40.50.1820)
  • S00374; Serine carboxypeptidase 2
    (Catalytic domain: 3.40.50.1820)
  • S00517; Lysosomal protective protein
    (Catalytic domain: 3.40.50.1820)
  • T00074; Prothrombin
    (Catalytic domain: 2.40.10.10)
  • T00410; Acrosin
    (Catalytic domain: 2.40.10.10)
  • T00411; Serine protease HTRA2
    (Catalytic domain: 2.40.10.10)
  • 1.13.30000.12Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismSer/Glu/Asp + Asp/mainchain amide
  • S00296; Pseudomonalisin
    (Catalytic domain: 3.40.50.200)
  • 1.13.30000.13Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismSer/Glu/Asp + Tyr/mainchain amide
  • D00210; Prolyl endopeptidase
    (Catalytic domain: 3.40.50.1820)
  • 1.13.30000.16Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismSer/Tyr/Lys + mainchain amide
  • S00512; Beta-lactamase
    (Catalytic domain: 3.40.710.10)
  • 1.13.30000.39Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismCys/His/Glu + Arg/mainchain amide
  • S00408; Pyrrolidone-carboxylate peptidase
    (Catalytic domain: 3.40.630.20)
  • 1.13.30000.41Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismCys/His/Asp + mainchain amide
  • M00216; Genome polyprotein
    (Catalytic domain: 2.40.10.10)
  • M00217; Genome polyprotein
    (Catalytic domain: 2.40.10.10)
  • 1.13.30000.42Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismCys/His/Glu + mainchain amide
  • M00217; Genome polyprotein
    (Catalytic domain: 2.40.10.10)
  • T00114; GMP synthase {glutamine-hydrolyzing}
    (Catalytic domains: 3.40.50.620, 3.40.50.880)
  • 1.13.30000.44Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismCys/His/Asn + Gln/mainchain amide
  • S00444; Cathepsin Z
    (Catalytic domain: 3.90.70.10)
  • S00445; Cathepsin B
    (Catalytic domain: 3.90.70.10)
  • S00446; Chymopapain
    (Catalytic domain: 3.90.70.10)
  • S00447; Actinidain (Actinidin) (EC 3.4.22.14)AltName: Allergen=Act c 1;
    (Catalytic domain: 3.90.70.10)
  • S00448; Cathepsin H
    (Catalytic domain: 3.90.70.10)
  • S00449; Papaya proteinase 4
    (Catalytic domain: 3.90.70.10)
  • S00450; Caricain
    (Catalytic domain: 3.90.70.10)
  • S00451; Cathepsin K
    (Catalytic domain: 3.90.70.10)
  • S00518; Cathepsin L1
    (Catalytic domain: 3.90.70.10)
  • 1.13.30000.45Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismCys/His/Asp + Gln/mainchain amide
  • S00524; Ubiquitin carboxyl-terminal hydrolase YUH1
    (Catalytic domain: 3.40.532.10)
  • 1.13.30000.46Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Trypsin-like mechanismCys/His/Tyr + mainchain amide
  • M00209; Genome polyprotein
    (Catalytic domain: 2.40.10.10)
  • 1.13.30005.18Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)DmpA-like mechanismSer(N-terminal) + N-terminal alpha-amine + Ser/mainchain amide + Asn/mainchain amide
  • S00528; D-aminopeptidase
    (Catalytic domain: 3.60.70.12)
  • 1.13.30005.34Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)DmpA-like mechanismThr(N-terminal) + N-terminal alpha-amine + Thr + mainchain amide
  • T00418; Gamma-glutamyltranspeptidase
    (Catalytic domain: 3.60.20.10)
  • 1.13.30010.35Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)20S-proteasome-like mechanismThr(N-terminal) + N-terminal alpha-amine + Lys/mainchain amide + acidic resiude
  • M00123; Proteasome component PRE5
    (Catalytic domain: 3.60.20.10)
  • M00174; Proteasome subunit alpha
    (Catalytic domain: 3.60.20.10)
  • 1.13.30010.30Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)20S-proteasome-like mechanismSer(N-terminal) + N-terminal alpha-amine + Asn/mainchain amide +His
  • M00309; Glutaryl-7-aminocephalosporanic-acid acylase
    (Catalytic domain: 3.60.20.10)
  • 1.13.30015.15Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)DD-carboxypeptidase-like mechanismSer/Lys/Tyr/Asn/His + mainchain amide
  • S00414; D-alanyl-D-alanine carboxypeptidase
    (Catalytic domain: 3.40.710.10)
  • T00222; D-aminopeptidase
    (Catalytic domain: 3.40.710.10)
  • 1.13.30020.28Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Tricorn protease-like mechanismSer/His/Glu/Ser + mainchain amide
  • M00351; Tricorn protease
    (Catalytic domains: 3.30.750.44, 3.90.226.10)
  • 1.13.30100.19Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Caspase-1-like mechanismSer/Lys + mainchain amide
  • D00510; LexA repressor
    (Catalytic domain: 2.10.109.10)
  • 1.13.30100.40Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Caspase-1-like mechanismCys/His + mainchain amide
  • D00522; Caspase-1
    (Catalytic domain: 3.40.50.1460)
  • 1.13.30100.49Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Caspase-1-like mechanismCys/Asp/Lys
  • S00341; N-carbamoylsarcosine amidase
    (Catalytic domain: 3.40.50.850)
  • 1.13.30110.56Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Asparagine-synthetase-B glutamine hydrolysis mechanismCys(N-terminal) + N-terminal alpha-amine + Asn/mainchain amide
  • D00300; Asparagine synthetase B {glutamine-hydrolyzing}
    (Catalytic domains: 3.40.50.620, 3.60.20.10)
  • T00201; Glucosamine--fructose-6-phosphate aminotransferase {isomerizing}
    (Catalytic domains: 3.40.50.10490, 3.60.20.10)
  • 1.13.30120.22Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Plasminogen-like mechanismSer/His/Asp
  • M00157; Plasminogen
    (Catalytic domain: 2.40.10.10)
  • 1.13.30185.55Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)Penicillin acylase-like mechanismCys(N-terminal) + N-terminal alpha-amine + Asn/mainchain amide + Arg/Asp
  • S00437; Penicillin acylase
    (Catalytic domain: 3.60.60.10)
  • 1.13.29990.17Hydrolysis Amide bond (One of Carbonyl Ester bonds; including peptide bond)beta-lactamase OXA10 mechanismSer/carbamated-Lys + Trp + mainchain amide
  • S00513; Beta-lactamase OXA-10
    (Catalytic domain: 3.40.710.10)
  • 1.14.200.137Hydrolysis Thioester (One of Carbonyl Ester bonds)Pepsin-like mechanismAn acidic residue + [Asn or Gln] + mainchain amide groups + Cys covalently-bound to substrate/intermediate
  • D00021; Betaine aldehyde dehydrogenase
    (Catalytic domains: 3.40.309.10, 3.40.605.10)
  • 1.14.800.129Hydrolysis Thioester (One of Carbonyl Ester bonds)GDP mannose dehydrogenase final step-like mechanismAn acidic residue + Lys/Asp/Thr
  • T00227; UDP-glucose 6-dehydrogenase
    (Catalytic domains: 1.10.1040.10, 3.40.50.720)
  • T00408; GDP-mannose 6-dehydrogenase
    (Catalytic domains: 1.-.-.-, 3.40.50.720)
  • 1.14.10105.1251Hydrolysis Thioester (One of Carbonyl Ester bonds)Glyoxalase-II-like mechanismAsp + two zinc ions bound to His/Asp cluster
  • S00431; Hydroxyacylglutathione hydrolase
    (Catalytic domain: 3.60.15.10)
  • 1.14.30000.10Hydrolysis Thioester (One of Carbonyl Ester bonds)Trypsin-like mechanismSer/His/Asp + mainchain amide
  • S00350; Palmitoyl-protein thioesterase 1
    (Catalytic domain: 3.40.50.1820)
  • S00919; Thioesterase
    (Catalytic domain: 3.40.50.1820)
  • 1.14.30000.45Hydrolysis Thioester (One of Carbonyl Ester bonds)Trypsin-like mechanismCys/His/Asp + Gln/mainchain amide
  • S00524; Ubiquitin carboxyl-terminal hydrolase YUH1
    (Catalytic domain: 3.40.532.10)
  • 1.14.36110.975Hydrolysis Thioester (One of Carbonyl Ester bonds)4-hydroxybenzoyl-CoA thioesterase-like mechanismTwo acidic residues + mainchain amide
  • S00179; 4-hydroxybenzoyl-CoA thioesterase
    (Catalytic domain: 3.10.129.10)
  • 1.15.7590.1491Hydrolysis Phosphoric EsterPP2Calpha phosphtase-like mechanismHis + acidic residue + Arg + two divalent ions bound to three acidic residues/mainchain carbonyl
  • D00152; Protein phosphatase 1A
    (Catalytic domain: 3.60.40.10)
  • 1.15.7910.1164Hydrolysis Phosphoric EsterInositol-1-monophosphatase-like mechanismAsp/Thr + mainchain amide groups + two magnesium ions bound to acidic residues/mainchain carbonyl
  • D00148; 3''(2''),5''-bisphosphate nucleotidase (EC 3.1.3.7) (3''
    (Catalytic domains: 3.30.540.10, 3.40.190.80)
  • D00153; Inositol-1-monophosphatase
    (Catalytic domains: 3.30.540.10, 3.40.190.80)
  • D00491; 3''(2''),5''-bisphosphate nucleotidase 1
    (Catalytic domains: 3.30.540.10, 3.40.190.80)
  • T00053; Inositol polyphosphate 1-phosphatase
    (Catalytic domains: 3.30.540.10, 3.40.190.80)
  • 1.15.8000.2150Hydrolysis Phosphoric EsterInorganic pyrophosphatase-like mechanismAsp/Arg/Lys/Tyr+ three magnesium ions bound to acidic residues
  • S00455; Inorganic pyrophosphatase
    (Catalytic domain: 3.90.80.10)
  • 1.15.8220.1169Hydrolysis Phosphoric EsterThermophilus ADPribose pyrophosphatase Ndx4-like mechanismAn acidic residue + Arg/Arg + two magnesium ions bound to acidic residues/mainchain carbonyl
  • S00922; ADP-ribose pyrophosphatase
    (Catalytic domain: 3.90.79.10)
  • 1.15.8230.361Hydrolysis Phosphoric EsterDNA topoisomerase-II ATP hydrolysis mechanismAn acidic residue + Lys + mainchain amide + magnesium ion bound to Asn
  • M00048; DNA topoisomerase 2
    (Catalytic domains: 3.30.230.10, 3.30.565.10, 3.40.50.670, 3.90.199.10)
  • M00213; DNA gyrase subunit B
    (Catalytic domains: 3.30.230.10, 3.30.565.10)
  • 1.15.8230.352Hydrolysis Phosphoric EsterDNA topoisomerase-II ATP hydrolysis mechanismAn acidic residue + Lys cluster + mainchain amide + magnesium ion bound to Ser & water molecules
  • M00114; Nitrogenase molybdenum-iron protein alpha chain
    (Catalytic domains: 3.40.50.300, 3.40.50.1980)
  • M00199; Nitrogenase molybdenum-iron protein alpha chain
    (Catalytic domains: 3.40.50.300, 3.40.50.1980)
  • 1.15.8230.362Hydrolysis Phosphoric EsterDNA topoisomerase-II ATP hydrolysis mechanismAn acidic residue + Arg + [Gln or Asn] + [Ser or Thr] + mainchain amide/carbonyl + magnesium ion bound directly to substrate
  • T00231; DUTP pyrophosphatase
    (Catalytic domain: 2.70.40.10)
  • 1.15.8230.371Hydrolysis Phosphoric EsterDNA topoisomerase-II ATP hydrolysis mechanismHis/Arg + magnesium ion bound to Asn
  • S00402; Nuclease
    (Catalytic domain: 3.40.570.10)
  • 1.15.8240.1166Hydrolysis Phosphoric EsterNuclease P1-like mechanismAn acidic residue + Lys cluster + two magnesium ions bound to acidic residues/mainchain carbonyl + another magnesium ion bound to leaving group
  • S00815; Bis(5''-nucleosyl)-tetraphosphatase {asymmetrical}
    (Catalytic domain: 3.90.79.10)
  • 1.15.8240.1167Hydrolysis Phosphoric EsterNuclease P1-like mechanismAn acidic residue + Arg/Lys + two magnesium ions bound to acidic residues/mainchain carbonyl + another magnesium ion bound to leaving group
  • S00920; Diadenosine 5'',5''''''-P1,P4-tetraphosphate hydrolase
    (Catalytic domain: 3.90.79.10)
  • 1.15.8240.2170Hydrolysis Phosphoric EsterNuclease P1-like mechanismAsp/Arg + two zinc ions bound to His/Asp cluster & mainchain (interacting throuhg a water) + zinc ion bound to His/Asp cluster
  • S00022; Nuclease P1
    (Catalytic domain: 1.10.575.10)
  • 1.15.8245.1168Hydrolysis Phosphoric EsterE.coli ADPribose pyrophosphatase-like mechanismAn acidic residue + Arg + three magnesium ions bound to acidic residues/mainchain carbonyl
  • S00814; ADP-ribose pyrophosphatase
    (Catalytic domain: 3.90.79.10)
  • S00921; MutT/nudix family protein
    (Catalytic domain: 3.90.79.10)
  • S00923; MutT/nudix family protein
    (Catalytic domain: 3.90.79.10)
  • S00924; ADP-sugar pyrophosphatase
    (Catalytic domain: 3.90.79.10)
  • 1.15.8300.1170Hydrolysis Phosphoric EsterBamHI-like mechanismGlu + two magnesium ions bound to acidic residues
  • S00384; Type-2 restriction enzyme BamHI
    (Catalytic domain: 3.40.91.20)
  • 1.15.8310.1250Hydrolysis Phosphoric EsterPhosphotriesterase-like mechanismAn acidic residue + two zinc ions bound to carbamated Lys & His/Asp cluster
  • S00231; Parathion hydrolase
    (Catalytic domain: 3.20.20.140)
  • 1.15.9400.1180Hydrolysis Phosphoric EsterType-II restriction enzyme-like mechanismLys + two magnesium ions bound to acidic residues
  • S00383; Type-2 restriction enzyme Cfr10I
    (Catalytic domain: 3.40.91.10)
  • S00390; Type-2 restriction enzyme PvuII
    (Catalytic domain: 3.40.210.10)
  • S00403; Type-2 restriction enzyme EcoRI
    (Catalytic domain: 3.40.580.10)
  • S00404; Type-2 restriction enzyme EcoRV
    (Catalytic domain: 3.40.600.10)
  • S00405; Type-2 restriction enzyme BglI
    (Catalytic domain: 3.40.600.20)
  • S00462; Exonuclease
    (Catalytic domain: 3.90.320.10)
  • T00055; Type-2 restriction enzyme FokI
    (Catalytic domain: 3.40.91.30)
  • 1.15.9500.580Hydrolysis Phosphoric EsterIron(III)-zinc(II) purple acid phosphatase-like mechanismHis/His + Fe(III)/Zinc2+ bound to Asp/His cluster
  • D00146; Fe(3+)-Zn(2+) purple acid phosphatase (PAP) (EC 3.1.3.2) (Iron
    (Catalytic domain: 3.60.21.10)
  • 1.15.9500.540Hydrolysis Phosphoric EsterIron(III)-zinc(II) purple acid phosphatase-like mechanismAsp/His + Fe(III)/Fe(II) bound to Asp/His cluster
  • S00435; Tartrate-resistant acid phosphatase type 5
    (Catalytic domain: 3.60.21.10)
  • 1.15.9995.1169Hydrolysis Phosphoric EsterHuman ADPribose pyrophosphatase NUDT9-like mechanismAn acidic residue + Arg/Arg + two magnesium ions bound to an acidic residue/mainchain carbonyl
  • D00880; ADP-ribose pyrophosphatase
    (Catalytic domain: 3.90.79.10)
  • 1.15.10100.1172Hydrolysis Phosphoric EsterDNA-topoisomerase-I/3'-5' exonuclease-like mechanismGlu/Tyr + two divalent metal ions bound to acidic residues
  • M00055; DNA polymerase I
    (Catalytic domains: 1.10.473.10, 3.30.70.370, 3.30.420.10)
  • 1.15.11200.472Hydrolysis Phosphoric EsterDeoxyribonuclease-1-like mechanismHis/His + divalent metal bound to Asp/Glu cluster
  • S00429; Deoxyribonuclease-1
    (Catalytic domain: 3.60.10.10)
  • 1.15.14410.1770Hydrolysis Phosphoric EsterSerine/threonine protein phosphatase 2B-like mechanismHis/Asp/Arg + Fe(III)/Zinc bound to Asp/His cluster
  • D00151; Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
    (Catalytic domain: 3.60.21.10)
  • 1.15.30200.84Hydrolysis Phosphoric EsterProstatic acid phosphatase-like mechanismHis/Asp + Arg/His cluster
  • D00514; Periplasmic AppA protein
    (Catalytic domain: 3.40.50.1240)
  • S00363; Prostatic acid phosphatase
    (Catalytic domain: 3.40.50.1240)
  • 1.15.30200.81Hydrolysis Phosphoric EsterProstatic acid phosphatase-like mechanismHis/His/Glu + Arg/Asn cluster
  • S00365; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
    (Catalytic domain: 3.40.50.1240)
  • 1.15.32000.86Hydrolysis Phosphoric Ester3-phytase-like mechanismHis/Asp
  • D00460; 3-phytase A
    (Catalytic domain: 3.40.50.1240)
  • 1.15.33000.50Hydrolysis Phosphoric EsterAdipocyte acid phosphatase-like mechanismCys/Asp/Arg
  • S00298; Low molecular weight phosphotyrosine protein phosphatase
    (Catalytic domain: 3.40.50.270)
  • 1.15.36030.52Hydrolysis Phosphoric EsterTyrosine-protein-phosphatase-like mechanismCys/Asp/Arg + mainchain amide group + Ser or Thr
  • D00154; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
    (Catalytic domain: 3.90.190.10)
  • M00149; Receptor-type tyrosine-protein phosphatase F
    (Catalytic domain: 3.90.190.10)
  • M00169; Tyrosine-protein phosphatase non-receptor type 13
    (Catalytic domain: 3.90.190.10)
  • S00458; Dual specificity protein phosphatase 3
    (Catalytic domain: 3.90.190.10)
  • T00221; Tyrosine-protein phosphatase non-receptor type 11
    (Catalytic domain: 3.90.190.10)
  • 1.15.36100.51Hydrolysis Phosphoric EsterM-phase-inducer-phosphatase-1-like mechanismCys/Glu/Arg
  • S00393; M-phase inducer phosphatase 1
    (Catalytic domain: 3.40.250.10)
  • 1.15.39000.353Hydrolysis Phosphoric EsterSER Ca(2+)-ATPase-1-like mechanismAsp + magnesium ion bound to Asp cluster
  • M00102; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
    (Catalytic domain: 3.40.50.1000)
  • 1.15.60000.82Hydrolysis Phosphoric EsterRibonuclease-U2-like mechanismHis/His + Acidic residue
  • S00467; Ribonuclease Rh
    (Catalytic domain: 3.90.730.10)
  • 1.15.60000.85Hydrolysis Phosphoric EsterRibonuclease-U2-like mechanismHis/Glu/Arg
  • S00174; Ribonuclease U2
    (Catalytic domain: 3.10.450.30)
  • S00175; Ribonuclease alpha-sarcin
    (Catalytic domain: 3.10.450.30)
  • 1.15.72000.565Hydrolysis Phosphoric EsterPhosphoinositide phospholipase-C-delta-1-like mechanismGlu/His + His/Glu/Asn + calcium ion bound to His/Asn + acidic residues
  • M00118; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1
    (Catalytic domain: 3.20.20.190)
  • M00183; Putative uncharacterized protein
    (Catalytic domain: 3.20.20.190)
  • 1.19.30000.41Hydrolysis Carboxylic Ester bond with conjugated double bondTrypsin-like mechanismCys/His/Asp + mainchain amide
  • S00348; Carboxymethylenebutenolidase
    (Catalytic domain: 3.40.50.1820)
  • 1.19.30000.10Hydrolysis Carboxylic Ester bond with conjugated double bondTrypsin-like mechanismSer/His/Asp + mainchain amide
  • D00144; Chemotaxis response regulator protein-glutamate methylesterase
    (Catalytic domain: 3.40.50.180)
  • 1.20.30800.980Hydrolysis Halide bondL-2-haloacid dehalogenase-like mechanismTwo acidic residues + Arg/Asn/Thr/Ser + Lys/Tyr
  • D00248; (S)-2-haloacid dehalogenase
    (Catalytic domains: 1.10.164.10, 3.40.50.1000)
  • 1.20.30810.950Hydrolysis Halide bondHalogenase-like mechanismAsp/His/Asp + mainchain amide + Trp/Trp
  • S00356; Haloalkane dehalogenase
    (Catalytic domain: 3.40.50.1820)
  • 1.20.30810.951Hydrolysis Halide bondHalogenase-like mechanismAsp/His/Glu + mainchain amide + Trp/Asn
  • S00525; Haloalkane dehalogenase
    (Catalytic domain: 3.40.50.1820)
  • 1.30.260.1001Hydrolysis C-O bond (O-glycoside bond)Endo-polygalacturonase-like mechanismFour acidic residues + Lys/Arg
  • S00168; Endo-polygalacturonase
    (Catalytic domain: 2.160.20.10)
  • 1.30.300.2Hydrolysis C-O bond (O-glycoside bond)Exoglucanase-II-like mechanismTwo acidic residues
  • D00536; Endoglucanase E-2
    (Catalytic domain: 3.20.20.40)
  • D00537; Exoglucanase 2
    (Catalytic domain: 3.20.20.40)
  • S00021; Lysozyme
    (Catalytic domain: 1.10.530.40)
  • S00194; Endoglucanase-6B
    (Catalytic domain: 3.20.20.40)
  • S00265; Chitosanase
    (Catalytic domain: 1.10.530.40)
  • 1.30.4920.981Hydrolysis C-O bond (O-glycoside bond)Glucoamylase-like mechanismTwo acidic residues + Arg/Tyr
  • D00500; Glucoamylase
    (Catalytic domain: 1.50.10.10)
  • 1.30.4950.1Hydrolysis C-O bond (O-glycoside bond)Goose-lysozyme-like mechanismAn acidic residue
  • S00520; Lysozyme g
    (Catalytic domain: 1.10.530.10)
  • 1.30.5050.991Hydrolysis C-O bond (O-glycoside bond)Cellulase9A-like mechanismThree acidic residues + Tyr/His
  • D00167; Cellulase Cel9-M
    (Catalytic domain: 1.50.10.10)
  • M00192; Endoglucanase E-4
    (Catalytic domain: 1.50.10.10)
  • S00531; Endo-b-1,4-glucanase (Cellulase NtEG)
    (Catalytic domain: 1.50.10.10)
  • T00246; Endoglucanase G
    (Catalytic domain: 1.50.10.10)
  • 1.30.5050.993Hydrolysis C-O bond (O-glycoside bond)Cellulase9A-like mechanismThree acidic residues + Tyr/Tyr
  • T00245; Endoglucanase D
    (Catalytic domain: 1.50.10.10)
  • 1.30.5100.2Hydrolysis C-O bond (O-glycoside bond)beta-amylase-like mechanismTwo acidic residues
  • D00166; Beta-amylase
    (Catalytic domain: 3.20.20.80)
  • S00125; Endoglucanase-5
    (Catalytic domain: 2.40.40.10)
  • 1.30.11370.560Hydrolysis C-O bond (O-glycoside bond)ER-Man(9)-alpha-mannosidase-like mechanismTwo acidic residues + calcium ion bound to Thr
  • D00470; Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
    (Catalytic domain: 1.50.10.50)
  • S00051; Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
    (Catalytic domain: 1.50.10.50)
  • 1.30.33100.2Hydrolysis C-O bond (O-glycoside bond)Lysozyme-C-like mechanismTwo acidic residues
  • S00509; Lysozyme C (EC 3.2.1.17) (1,4-beta-N-acetylmuramidase C) (Allergen Gal d IV)AltName: Allergen=Gal d 4;
    (Catalytic domain: 1.10.530.10)
  • 1.30.35880.983Hydrolysis C-O bond (O-glycoside bond)beta-galactanase-like mechanismTwo acidic residues + Arg/Tyr + Asn or Gln
  • S00748; Arabinogalactan endo-1,4-beta-galactosidase
    (Catalytic domain: 3.20.20.80)
  • S00912; Arabinogalactan endo-1,4-beta-galactosidase
    (Catalytic domain: 3.20.20.80)
  • 1.30.35885.972Hydrolysis C-O bond (O-glycoside bond)beta-xylosidase-like mechanismTwo acidic residues + Arg/His/Tyr + Asn or Gln
  • D00501; Endoglucanase Z
    (Catalytic domain: 3.20.20.80)
  • D00502; Endoglucanase E1
    (Catalytic domain: 3.20.20.80)
  • D00503; Endoglucanase A
    (Catalytic domain: 3.20.20.80)
  • D00844; Beta-xylosidase
    (Catalytic domain: 3.20.20.80)
  • D00861; Mannanase
    (Catalytic domain: 3.20.20.80)
  • D00864; Glucosylceramidase
    (Catalytic domain: 3.20.20.80)
  • S00203; Endoglucanase C
    (Catalytic domain: 3.20.20.80)
  • S00208; Glucan 1,3-beta-glucosidase
    (Catalytic domain: 3.20.20.80)
  • S00210; Mannan endo-1,4-beta-mannosidase 4
    (Catalytic domain: 3.20.20.80)
  • S00906; Beta-mannanase
    (Catalytic domain: 3.20.20.80)
  • S00907; Mannan endo-1,4-beta-mannosidase
    (Catalytic domain: 3.20.20.80)
  • 1.30.35890.994Hydrolysis C-O bond (O-glycoside bond)alpha-N-acetylgalactosaminidase-like mechanismThree acidic residues + Arg/Tyr
  • D00664; Alpha-galactosidase
    (Catalytic domain: 3.20.20.70)
  • D00665; Alpha-N-acetylgalactosaminidase
    (Catalytic domain: 3.20.20.70)
  • D00863; Alpha-galactosidase
    (Catalytic domain: 3.20.20.70)
  • 1.30.36000.3Hydrolysis C-O bond (O-glycoside bond)alpha-amylase-like mechanismThree acidic residues
  • D00165; Alpha-amylase type B isozyme
    (Catalytic domain: 3.20.20.80)
  • D00176; Glucan 1,4-alpha-maltotetraohydrolase
    (Catalytic domain: 3.20.20.80)
  • M00112; Maltogenic alpha-amylase
    (Catalytic domain: 3.20.20.80)
  • M00193; Neopullulanase 2
    (Catalytic domain: 3.20.20.80)
  • T00057; Alpha-amylase
    (Catalytic domain: 3.20.20.80)
  • T00062; Oligo-1,6-glucosidase
    (Catalytic domain: 3.20.20.80)
  • T00067; Isoamylase
    (Catalytic domain: 3.20.20.80)
  • 1.30.36010.970Hydrolysis C-O bond (O-glycoside bond)Exoglucanase/xylanase-A-like mechanismTwo acidic residues + His/Asp
  • D00169; Endo-1,4-beta-xylanase A
    (Catalytic domain: 3.20.20.80)
  • D00479; Exoglucanase/xylanase
    (Catalytic domain: 3.20.20.80)
  • M00160; Endo-1,4-beta-xylanase A
    (Catalytic domain: 3.20.20.80)
  • 1.30.36020.973Hydrolysis C-O bond (O-glycoside bond)Gentiobiase-like mechanismTwo acidic residues + His/Tyr
  • S00205; Beta-glucosidase A
    (Catalytic domain: 3.20.20.80)
  • 1.30.36040.982Hydrolysis C-O bond (O-glycoside bond)beta-D-glucan-exohydrolase-like mechanismTwo acidic residues + Trp/Trp/Arg
  • D00175; Beta-D-glucan exohydrolase isoenzyme ExoI
    (Catalytic domains: 3.20.20.300, 3.40.50.1700)
  • 1.30.36200.973Hydrolysis C-O bond (O-glycoside bond)Lactase-like mechanismTwo acidic residues + His/Tyr
  • S00206; Beta-galactosidase
    (Catalytic domain: 3.20.20.80)
  • 1.30.36027.984Hydrolysis C-O bond (O-glycoside bond)beta-mannanase26-like mechanismTwo acidic residues + Trp/Arg/Tyr/His/Trp
  • M00346; Man26A
    (Catalytic domain: 3.20.20.80)
  • S00911; Beta-1,4-mannanase
    (Catalytic domain: 3.20.20.80)
  • S00915; Endo-1
    (Catalytic domain: 3.20.20.80)
  • 1.30.36210.971Hydrolysis C-O bond (O-glycoside bond)Sialidase-like mechanismTwo acidic residues + Tyr
  • S00207; Glucan endo-1,3-beta-glucosidase GII
    (Catalytic domain: 3.20.20.80)
  • S00209; Lichenase-2
    (Catalytic domain: 3.20.20.80)
  • S00211; 6-phospho-beta-galactosidase
    (Catalytic domain: 3.20.20.80)
  • T00066; Beta-glucuronidase
    (Catalytic domain: 3.20.20.80)
  • 1.30.36210.990Hydrolysis C-O bond (O-glycoside bond)Sialidase-like mechanismThree acidic residues + His
  • S00162; Endoglucanase 1
    (Catalytic domain: 2.70.100.10)
  • 1.30.36211.974Hydrolysis C-O bond (O-glycoside bond)Endoglucanase-E1-like mechanismTwo acidic residues + Tyr/Tyr
  • D00538; Endo-1,4-beta-xylanase
    (Catalytic domain: 2.60.120.180)
  • S00151; Endo-1,4-beta-xylanase
    (Catalytic domain: 2.60.120.180)
  • 1.30.36211.992Hydrolysis C-O bond (O-glycoside bond)Endoglucanase-E1-like mechanismThree acidic residues + Asn or Gln
  • D00504; Cellulase B
    (Catalytic domain: 2.60.120.180)
  • S00150; Cellulase
    (Catalytic domain: 2.60.120.180)
  • 1.30.36211.1000Hydrolysis C-O bond (O-glycoside bond)Endoglucanase-E1-like mechanismFour acidic residues
  • S00533; Endoglucanase A
    (Catalytic domain: 2.60.120.180)
  • 1.30.36300.2Hydrolysis C-O bond (O-glycoside bond)Endo-beta-(1->3)-(1->4) glucanase-like mechanismTwo acidic residues
  • S00511; Beta-glucanase
    (Catalytic domain: 2.60.120.200)
  • 1.30.46030.482Hydrolysis C-O bond (O-glycoside bond)alpha-mannosidase-like mechanismTwo acidic residues + Arg/Tyr + zinc ion bound to His/acidic residue + substrate/intermediate
  • M00314; Alpha-mannosidase
    (Catalytic domains: 1.-.-.-, 3.20.110.10)
  • 1.31.36210.99Hydrolysis C-O bond (O-glycoside bond) adjacent to carboxylateSialidase-like mechanismTyr + two acidic residues
  • T00065; Sialidase
    (Catalytic domain: 2.120.10.10)
  • T00208; Sialidase
    (Catalytic domain: 2.120.10.10)
  • 1.32.5000.73Hydrolysis C-O bond (O-glycoside bond) close to N-acetyl groupChitinase-A-like mechanismGlu/Tyr/Asp
  • M00134; Chitinase A1
    (Catalytic domain: 3.20.20.80)
  • T00063; Chitinase A
    (Catalytic domain: 3.20.20.80)
  • 1.32.60200.73Hydrolysis C-O bond (O-glycoside bond) close to N-acetyl groupHevamine-A-like mechanismGlu/Tyr/Asp
  • S00204; Hevamine-A
    (Catalytic domain: 3.20.20.80)
  • S00213; Endo-beta-N-acetylglucosaminidase H
    (Catalytic domain: 3.20.20.80)
  • 1.32.68200.2Hydrolysis C-O bond (O-glycoside bond) close to N-acetyl groupChitobiase-like mechanismTwo acidic residues
  • M00026; Chitobiase
    (Catalytic domain: 3.20.20.80)
  • 1.32.68230.73Hydrolysis C-O bond (O-glycoside bond) close to N-acetyl groupHyaluronidase-like mechanismGlu/Tyr/Asp
  • D00804; Hyaluronidase-1
    (Catalytic domain: 3.20.20.70)
  • S00745; Hyaluronidase A (Hya A) (EC 3.2.1.35) (Allergen Ves v II) (Hyaluronoglucosaminidase A)AltName: Allergen=Ves v 2a;
    (Catalytic domain: 3.20.20.70)
  • 1.35.30000.63Hydrolysis C-O bond (epoxide; cyclic ether)Trypsin-like mechanismAsp/His/Asp/Tyr + mainchain amide
  • D00539; Bifunctional epoxide hydrolase 2
    (Catalytic domain: 3.40.50.1820)
  • S00352; Epoxide hydrolase
    (Catalytic domain: 3.40.50.1820)
  • 1.40.300.2Hydrolysis C-N bond (N-glycoside bond)Exoglucanase-II-like mechanismTwo acidic residues
  • S00188; DNA-3-methyladenine glycosylase
    (Catalytic domain: 3.10.300.10)
  • 1.40.4930.61Hydrolysis C-N bond (N-glycoside bond)Uracil-DNA glycosylase-like mechanismAsp/His
  • S00401; Uracil-DNA glycosylase
    (Catalytic domain: 3.40.470.10)
  • 1.40.6430.132Hydrolysis C-N bond (N-glycoside bond)AlkA-DNA-glycosylase-like mechanismAn acidic residue + Tyr/Trp
  • T00070; DNA-3-methyladenine glycosylase 2
    (Catalytic domains: 1.10.340.30, 1.10.1670.10)
  • 1.40.7050.132Hydrolysis C-N bond (N-glycoside bond)TAG-DNA-glycosylase-like mechanismAn acidic residue + Tyr/Trp
  • S00749; DNA-3-methyladenine glycosylase 1
    (Catalytic domain: 1.10.340.30)
  • 1.40.14550.574Hydrolysis C-N bond (N-glycoside bond)IU-nucleoside hydrolase-like mechanismHis/Asp + calcium ion bound to acidic residues
  • S00461; Inosine-uridine preferring nucleoside hydrolase
    (Catalytic domain: 3.90.245.10)
  • S00751; Pyrimidine-specific ribonucleoside hydrolase RihB
    (Catalytic domain: 3.90.245.10)
  • 1.51.3100.78Hydrolysis C-C bond (Ketonic Carbon-Carbon bond; Carbonyl Carbon-Carbon adjacent to sp2 carbon)C-C hydrolase-like hydrolysis mechanismHis/Ser/Asp
  • S00355; 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
    (Catalytic domain: 3.40.50.1820)
  • S00526; 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
    (Catalytic domain: 3.40.50.1820)
  • 1.51.790.101Hydrolysis C-C bond (Ketonic Carbon-Carbon bond; Carbonyl Carbon-Carbon adjacent to sp2 carbon)Beta-ketoacyl-ACP synthase II 2nd reaction step-like mechanismHis/His/Lys
  • D00871; 3-oxoacyl-[acyl-carrier-protein] synthase 2
    (Catalytic domain: 3.40.47.10)
  • 1.60.55100.9000Hydrolysis C-S bond (S-glycoside bond)Myrosinase-MA1-like mechanismGlu/Tyr + Ascorbate bound to Gln
  • S00214; Myrosinase MA1
    (Catalytic domain: 3.20.20.80)
  • 2.15.32480.334Phosphorolysis Phosphoric EsterStabilizer; SN2-like nucleophilic attack on covalent bond by phosphate; Cofactor-assisted stabilization of SN2-like transition state mechanismArg/Lys cluster + mainchain amide + magnesium ion bound to acidic residues
  • S00465; Glucose-1-phosphate thymidylyltransferase 1
    (Catalytic domain: 3.90.550.10)
  • 2.40.18000.65Phosphorolysis C-N bond (N-glycoside bond)Acid/Stabilizer; SN1-like transition state; nucleophilic attack by phosphate group; Protonation to leaving group by acid mechanismAsp + negatively charged residues
  • S00376; S-methyl-5''-thioadenosine phosphorylase
    (Catalytic domain: 3.40.50.1580)
  • S00510; Purine nucleoside phosphorylase deoD-type
    (Catalytic domain: 3.40.50.1580)
  • 2.40.18500.20Phosphorolysis C-N bond (N-glycoside bond)Stabilizer; SN1-like transition state; nucleophilic attack by phosphate group mechanismAsn/[Glu or Asp] + negatively charged residues
  • S00375; Purine nucleoside phosphorylase
    (Catalytic domain: 3.40.50.1580)
  • 2.40.48000.390Phosphorolysis C-N bond (N-glycoside bond)Stabilizer; SN1-like transition state; nucleophilic attack by phosphate group; cofactor-assisted mechanismLys/Arg + magnesium ion bound
  • S00288; Orotate phosphoribosyltransferase
    (Catalytic domain: 3.40.50.2020)
  • 3.100.790.76Transfer Phosphate group from Hydroxyl oxygen to Hydroxyl oxygen Bacterial PI-PLC-like mechanismHis/His + acidic residues + Arg
  • S00236; 1-phosphatidylinositol phosphodiesterase
    (Catalytic domain: 3.20.20.190)
  • 3.100.219000.96Transfer Phosphate group from Hydroxyl oxygen to Hydroxyl oxygen DNA topoisomerase III autotransfer mechanismTyr/Glu + Arg/Lys cluster
  • M00158; DNA topoisomerase 3
    (Catalytic domains: 1.10.290.10, 3.40.50.140)
  • 3.100.219010.98Transfer Phosphate group from Hydroxyl oxygen to Hydroxyl oxygen DNA topoisomerase I autotransfer mechanismTyr/Glu/His/Asp + Arg/Lys cluster
  • M00034; DNA topoisomerase 1
    (Catalytic domains: 1.10.290.10, 3.40.50.140)
  • 3.100.220195.81Transfer Phosphate group from Hydroxyl oxygen to Hydroxyl oxygen Cofactor-dependent-phosphoglycerate-mutase-like mechanismHis/His/Glu + Arg/Asn cluster
  • S00366; 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase
    (Catalytic domain: 3.40.50.1240)
  • 3.100.305000.501Transfer Phosphate group from Hydroxyl oxygen to Hydroxyl oxygen DNA topoisomerase II autotransfer mechanismTyr + Acidic residues + Arg cluster + magnesium ion bound to acidic residues
  • M00048; DNA topoisomerase 2
    (Catalytic domains: 3.30.230.10, 3.30.565.10, 3.40.50.670, 3.90.199.10)
  • 3.103.69900.359Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen 6-phosphofructokinase-like mechanismAsp/Asp + Arg/Thr + mainchain amide + magnesium ion bound to acidic residues
  • D00111; 6-phosphofructokinase
    (Catalytic domains: 3.40.50.450, 3.40.50.460)
  • 3.103.69910.364Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Deoxycytidine kinase-like mechanismAn acidic residue + Arg/Lys cluster + mainchain amide groups + magnesium ion bound to acidic residue and [Ser or Thr]
  • S00672; Deoxynucleoside kinase
    (Catalytic domain: 3.40.50.300)
  • S00682; Deoxycytidine kinase
    (Catalytic domain: 3.40.50.300)
  • 3.103.70000.350Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Thymidine kinase-like mechanismAn acidic residue + Arg/Lys cluster + magnesium ion bound to acidic residue and [Ser or Thr]
  • S00302; Thymidine kinase
    (Catalytic domain: 3.40.50.300)
  • 3.103.70000.357Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Thymidine kinase-like mechanismAsp/Arg + mainchain amide + magnesium ion bound
  • D00416; Adenosine kinase
    (Catalytic domains: 3.30.1110.10, 3.40.1190.20)
  • 3.103.70020.355Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen N-acylneuraminate cytidylyltransferase-like mechanismAn acidic residue + Arg/Lys cluster + magnesium ion bound to acidic residues
  • S00466; N-acylneuraminate cytidylyltransferase
    (Catalytic domain: 3.90.550.10)
  • 3.103.70035.351Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Shikimate kinase-II-like mechanismAn acidic residue + Lys cluster + mainchain amide groups + magnesium ion bound to Asp/Ser
  • D00116; Phosphatidylinositol 5-phosphate 4-kinase type-2 beta
    (Catalytic domains: 3.30.800.10, 3.30.810.10)
  • 3.103.70035.355Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Shikimate kinase-II-like mechanismAn acidic residue + Arg/Lys cluster + magnesium ion bound to acidic residues
  • S00301; Phosphoribulokinase 1
    (Catalytic domain: 3.40.50.300)
  • 3.103.70035.360Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Shikimate kinase-II-like mechanismAn acidic residue + Arg/Lys cluster + mainchain amide groups + magnesium ion bound to [Ser or Thr]
  • S00304; Shikimate kinase 2
    (Catalytic domain: 3.40.50.300)
  • S00554; Shikimate kinase 1
    (Catalytic domain: 3.40.50.300)
  • S00555; Shikimate kinase
    (Catalytic domain: 3.40.50.300)
  • S00680; Uridine-cytidine kinase 2
    (Catalytic domain: 3.40.50.300)
  • 3.103.70035.364Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Shikimate kinase-II-like mechanismAn acidic residue + Arg/Lys cluster + mainchain amide groups + magnesium ion bound to acidic residue and [Ser or Thr]
  • S00676; Dephospho-CoA kinase
    (Catalytic domain: 3.40.50.300)
  • S00913; Dephospho-CoA kinase
    (Catalytic domain: 3.40.50.300)
  • 3.103.70035.366Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Shikimate kinase-II-like mechanismAn acidic residue + Lys (cluster) + mainchain amide groups + magnesium ion bound to [Asn or Gln] and [Ser or Thr]
  • S00914; Dephospho-CoA kinase
    (Catalytic domain: 3.40.50.300)
  • 3.103.70035.456Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Shikimate kinase-II-like mechanismAn acidic residue + mainchain amide groups + zinc ion bound + potassium ion bound to acidic residues/Ser or Thr/mainchain carbonyl
  • S00678; Pyridoxal kinase
    (Catalytic domain: 3.40.1190.20)
  • 3.103.70200.1160Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Poly(A) polymerase-alpha-like mechanismAn acidic residue + two magnesium ions bound to acidic residues
  • M00218; Poly(A) polymerase alpha
    (Catalytic domain: 3.30.460.10)
  • T00202; Poly(A) polymerase
    (Catalytic domain: 3.30.460.10)
  • 3.103.70800.502Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Adenylyl-sulfate kinase-like mechanismTyr/Ser + Lys cluster + magnesium ion bound to acidic residue/Ser or Thr
  • S00303; Adenylyl-sulfate kinase
    (Catalytic domain: 3.40.50.300)
  • 3.103.70810.363Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Glucokinase-like mechanismAn acidic residue + Arg + magnesium ion bound to substrate/water
  • D00643; Glucokinase
    (Catalytic domain: 3.40.367.20)
  • 3.103.75410.402Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Gluconate kinase-like mechanismLys/Arg + mainchain amide groups + magnesium ion bound to Ser & acidic residues through water
  • S00671; Thermoresistant gluconokinase
    (Catalytic domain: 3.40.50.300)
  • 3.103.78000.1130Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen HPP kinase-like mechanismArg cluster + two magnesium ions bound to acidic residues
  • S00258; 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
    (Catalytic domain: 3.30.70.560)
  • 3.103.78010.320Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Hydroxyethylthiazole-kinase-like mechanismmainchain amide + magnesium ion bound to acidic residues
  • S00453; Hydroxyethylthiazole kinase
    (Catalytic domain: 3.40.1190.20)
  • 3.103.78020.387Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Pyridoxal kinase-like mechanismLys + mainchain amide groups + magnesium bound to acidic residues and mainchain carbonyl
  • S00905; Phosphomethylpyrimidine kinase
    (Catalytic domain: 3.40.1190.20)
  • 3.103.80570.321Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen HMP kinase 1st reaction step-like mechanismmainchain amide groups + magnesium ion bound to Thr
  • S00705; Phosphomethylpyrimidine kinase
    (Catalytic domain: 3.40.1190.20)
  • 3.103.90000.335Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Gro-PCT-like mechanismArg/His/Gln cluster + mainchain amide + magnesium ion bound
  • D00302; RNA 3''-terminal phosphate cyclase
    (Catalytic domain: 3.65.10.20)
  • 3.103.90020.1131Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen DNA polymerase-like mechanismArg/Lys/Asn + two magnesium ions bound to acidic residues
  • M00019; DNA polymerase
    (Catalytic domains: 1.10.287.280, 3.30.70.270)
  • 3.103.90020.1134Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen DNA polymerase-like mechanismArg/Lys/Asn + two magnesium ions bound to acidic residues/Ser/mainchain carbonyl
  • M00020; DNA polymerase
    (Catalytic domains: 1.-.-.-, 3.90.-.-)
  • 3.103.90020.1135Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen DNA polymerase-like mechanismArg/Lys/His/Tyr + two magnesium ions bound to acidic residues/mainchain carbonyl
  • M00173; DNA polymerase
    (Catalytic domains: 1.10.150.20, 3.30.70.370)
  • 3.103.90020.1136Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen DNA polymerase-like mechanismArg/Lys + two magnesium ions bound to acidic residues/mainchain carbonyl
  • M00104; DNA-directed RNA polymerase
    (Catalytic domains: 1.10.150.20, 3.30.70.370)
  • M00200; Adenylate cyclase type 5
    (Catalytic domain: 3.30.70.1230)
  • 3.103.90020.1140Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen DNA polymerase-like mechanismArg/Lys + mainchain amide + magnesium ion bound to Thr + a divalent metal ion bound to His/Asp/Lys
  • T00084; Phosphoenolpyruvate carboxykinase {ATP}
    (Catalytic domains: 2.170.8.10, 3.40.449.10, 3.90.228.20)
  • 3.103.90020.1141Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen DNA polymerase-like mechanismArg/Lys/His + mainchain amide groups + two divalent metal ions bound to acidic residues/mainchain carbonyl
  • M00055; DNA polymerase I
    (Catalytic domains: 1.10.473.10, 3.30.70.370, 3.30.420.10)
  • M00175; DNA polymerase I
    (Catalytic domains: 1.10.473.10, 3.30.70.370, 3.40.50.1010)
  • 3.103.90021.1120Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Reverse transcriptase-like mechanismmainchain amide groups + two magnesium ions bound to acidic residues
  • M00135; Gag-Pol polyprotein
    (Catalytic domains: 3.30.70.270, 3.30.420.10)
  • M00166; Gag-Pol polyprotein
    (Catalytic domains: 2.40.70.10, 3.30.70.270, 3.30.420.10)
  • 3.103.106000.1163Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen PI3-kinase-p110-like mechanismAsp/His + Ser/Lys + two magnesium ions bound to Asp/Asn
  • M00043; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
    (Catalytic domains: 1.10.1070.11, 3.30.1010.10)
  • 3.103.126600.1165Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Phosphorylase-kinase-like mechanismAsp + Lys cluster + mainchain amide + two magnesium ions bound to Asn/Asp
  • M00196; Cyclin-dependent kinase 5
    (Catalytic domains: 1.10.510.10, 3.30.200.20)
  • M00197; Casein kinase II subunit alpha
    (Catalytic domains: 1.10.510.10, 3.30.200.20)
  • M00198; Phosphorylase b kinase regulatory subunit alpha
    (Catalytic domains: 1.10.510.10, 3.30.200.20)
  • 3.103.130000.358Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Insulin receptor-like mechanismAsp/Arg + magnesium ion bound
  • M00136; Insulin-like growth factor 1 receptor (EC 2.7.10.1) (Insulin-like growth factor I receptor) (IGF-I receptor)AltName: CD_antigen=CD221;
    (Catalytic domain: 1.10.510.10)
  • 3.103.130000.1161Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Insulin receptor-like mechanismAsp/Lys + two magnesium ions bound to Asp/Asn
  • M00125; Beta-adrenergic receptor kinase 1
    (Catalytic domain: 1.10.510.10)
  • T00224; TGF-beta receptor type-1
    (Catalytic domain: 1.10.510.10)
  • 3.103.130000.1162Transfer Phosphate group from Phosphate oxygen to Hydroxyl oxygen Insulin receptor-like mechanismAsp/Arg + two magnesium ions bound to Asp/Asn
  • M00124; Ephrin type-B receptor 2
    (Catalytic domain: 1.10.510.10)
  • M00127; Fibroblast growth factor receptor 1 (FGFR-1) (EC 2.7.10.1) (Basic fibroblast growth factor receptor 1) (BFGFR) (bFGF-R-1) (Fms-like tyrosine kinase 2) (FLT-2) (N-sam) (Proto-oncogene c-Fgr)AltName: CD_antigen=CD331;
    (Catalytic domain: 1.10.510.10)
  • M00129; Insulin receptor (IR) (EC 2.7.10.1)AltName: CD_antigen=CD220;
    (Catalytic domain: 1.10.510.10)
  • M00130; Tyrosine-protein kinase ABL1
    (Catalytic domain: 1.10.510.10)
  • M00131; Vascular endothelial growth factor receptor 1
    (Catalytic domain: 1.10.510.10)
  • M00132; High affinity nerve growth factor receptor
    (Catalytic domain: 1.10.510.10)
  • M00304; Tyrosine-protein kinase CSK
    (Catalytic domain: 1.10.510.10)
  • M00323; Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Piebald trait protein) (PBT) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (p145 c-kit) (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog)AltName: CD_antigen=CD117;
    (Catalytic domain: 1.10.510.10)
  • M00325; Angiopoietin-1 receptor (EC 2.7.10.1) (Endothelial tyrosine kinase) (Tunica interna endothelial cell kinase) (Tyrosine kinase with Ig and EGF homology domains-2) (Tyrosine-protein kinase receptor TEK) (Tyrosine-protein kinase receptor TIE-2) (hTIE2) (p140 TEK)AltName: CD_antigen=CD202b;
    (Catalytic domain: 1.10.510.10)
  • M00326; Proto-oncogene tyrosine-protein kinase receptor Ret
    (Catalytic domain: 1.10.510.10)
  • M00327; Muscle
    (Catalytic domain: 1.10.510.10)
  • M00328; ALK tyrosine kinase receptor (EC 2.7.10.1) (Anaplastic lymphoma kinase)AltName: CD_antigen=CD246;
    (Catalytic domain: 1.10.510.10)
  • M00329; Macrophage-stimulating protein receptor (MSP receptor) (EC 2.7.10.1) (CDw136) (Protein-tyrosine kinase 8) (p185-Ron)AltName: CD_antigen=CD136;
    (Catalytic domain: 1.10.510.10)
  • M00330; Macrophage colony-stimulating factor 1 receptor (CSF-1 receptor) (CSF-1-R) (CSF-1R) (M-CSF-R) (EC 2.7.10.1) (Proto-oncogene c-Fms)AltName: CD_antigen=CD115;
    (Catalytic domain: 1.10.510.10)
  • M00331; Receptor-type tyrosine-protein kinase FLT3 (EC 2.7.10.1) (FL cytokine receptor) (Fetal liver kinase-2) (FLK-2) (Fms-like tyrosine kinase 3) (FLT-3) (Stem cell tyrosine kinase 1) (STK-1)AltName: CD_antigen=CD135;
    (Catalytic domain: 1.10.510.10)
  • M00332; Tyrosine-protein kinase Mer
    (Catalytic domain: 1.10.510.10)
  • M00333; Tyrosine-protein kinase ZAP-70
    (Catalytic domain: 1.10.510.10)
  • M00335; Activated CDC42 kinase 1
    (Catalytic domain: 1.10.510.10)
  • M00339; Tyrosine-protein kinase Fes/Fps
    (Catalytic domain: 1.10.510.10)
  • M00344; Cytoplasmic tyrosine-protein kinase BMX
    (Catalytic domain: 1.10.510.10)
  • 3.105.230000.86Transfer Phosphate group from Imine nitrogen (sp2) to Hydroxyl oxygen PTS system-mannose-specific IIAB-like mechanismHis/Asp
  • D00527; PTS system mannose-specific EIIAB component
    (Catalytic domain: 3.40.50.510)
  • 3.105.250000.90Transfer Phosphate group from Imine nitrogen (sp2) to Hydroxyl oxygen PTS system-like mechanismHis + His/Arg
  • S00046; Lactose-specific phosphotransferase enzyme IIA component
    (Catalytic domain: 1.20.58.80)
  • S00420; Nitrogen regulatory protein
    (Catalytic domain: 3.40.930.10)
  • T00258; PTS system mannitol-specific EIICBA component
    (Catalytic domain: 3.40.930.10)
  • 3.105.250000.91Transfer Phosphate group from Imine nitrogen (sp2) to Hydroxyl oxygen PTS system-like mechanismHis + amide (dipole)
  • S00283; Fructose-specific phosphotransferase enzyme IIB component
    (Catalytic domain: 3.40.35.10)
  • 3.105.250000.48Transfer Phosphate group from Imine nitrogen (sp2) to Hydroxyl oxygen PTS system-like mechanismCys/Arg
  • D00525; PTS system glucose-specific EIICB component
    (Catalytic domain: 3.30.1360.60)
  • S00297; N,N''-diacetylchitobiose-specific phosphotransferase enzyme IIB component
    (Catalytic domain: 3.40.50.270)
  • 3.110.90030.330Transfer Phosphate group from Hydroxyl oxygen to Carboxyl oxygen Thymidylate-kinase-like mechanismArg cluster + His + magnesium ion bound to acidic residues
  • T00217; Phosphoenolpyruvate carboxylase
    (Catalytic domains: 1.20.1100.10, 3.20.20.60)
  • 3.113.90000.331Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen Gro-PCT-like mechanismArg/Gln + magnesium ion bound to acidic residues
  • S00413; Aspartate--ammonia ligase
    (Catalytic domain: 3.30.930.10)
  • 3.113.90000.394Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen Gro-PCT-like mechanismLys/Arg cluster + magnesium ion bound to an acidic residue
  • M00049; Threonyl-tRNA synthetase
    (Catalytic domain: 3.30.930.10)
  • 3.113.90000.397Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen Gro-PCT-like mechanismLys/His/Asn + mainchain amide + magnesium ion bound
  • M00178; Glutaminyl-tRNA synthetase
    (Catalytic domains: 1.10.1160.10, 3.40.50.620)
  • 3.113.90020.1181Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen DNA polymerase-like mechanismLys/Asn + two magnesium ions bound to His/Glu/Ser
  • T00109; UDP-N-acetylmuramoylalanine--D-glutamate ligase
    (Catalytic domain: 3.40.1190.10)
  • 3.113.90020.1182Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen DNA polymerase-like mechanismLys + two magnesium ions bound to His/Glu/mainchain carbonyl
  • D00516; Folylpolyglutamate synthase
    (Catalytic domain: 3.40.1190.10)
  • 3.113.90030.330Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen Thymidylate-kinase-like mechanismArg cluster + His + magnesium ion bound to acidic residues
  • D00291; Lysyl-tRNA synthetase
    (Catalytic domain: 3.30.930.10)
  • 3.113.90030.1133Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen Thymidylate-kinase-like mechanismArg/Lys cluster + mainchain amide + two magnesium ions bound to acidic residues/Asn/Gln
  • M00051; Glutathione synthetase
    (Catalytic domains: 1.10.1080.10, 3.30.470.20, 3.30.1490.50)
  • T00107; Glutathione synthetase
    (Catalytic domains: 3.30.470.20, 3.30.1490.20)
  • 3.113.90030.2130Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen Thymidylate-kinase-like mechanismArg cluster + three magnesium ions bound to acidic residues
  • D00295; Glycyl-tRNA synthetase
    (Catalytic domain: 3.30.930.10)
  • 3.113.90030.2131Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen Thymidylate-kinase-like mechanismArg cluster + three magnesium ions bound to acidic residue/[Ser or Thr]
  • D00293; Seryl-tRNA synthetase
    (Catalytic domain: 3.30.930.10)
  • 3.113.90040.334Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen D-alanine--poly(phosphoribitol) ligase 1st reaction step-like mechanismArg/Lys cluster + mainchain amide + magnesium ion bound to acidic residues
  • M00347; D-alanine--poly(phosphoribitol) ligase subunit 1
    (Catalytic domains: 2.30.38.10, 3.30.300.30, 3.40.50.980)
  • 3.113.163000.1110Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen NH(3)-dependent NAD+ synthetase phosphoryl transfer mechanismMonovalent cation bound + magnesium ions bound to acidic residues
  • S00315; NH(3)-dependent NAD(+) synthetase
    (Catalytic domain: 3.40.50.620)
  • 3.113.310400.385Transfer Phosphate group from Phosphate oxygen to Carboxyl oxygen Succinyl-CoA synthetase phosphoryl transfer mechanismHis/Acidic residue + Arg/Thr + mainchain amide + magnesium bound to acidic residue/Asn or Gln
  • M00035; Succinyl-CoA ligase [GDP-forming] subunit alpha
    (Catalytic domains: 3.30.470.20, 3.30.1490.20, 3.40.50.261)
  • 3.123.90030.312Transfer Phosphate group from Phosphate oxygen to Carbonyl oxygen Thymidylate-kinase-like mechanismSer/Gly cluster + Lys + magnesium ion bound to acidic residues
  • T00114; GMP synthase {glutamine-hydrolyzing}
    (Catalytic domains: 3.40.50.620, 3.40.50.880)
  • 3.130.90020.2100Transfer Phosphate group from Hydroxyl oxygen to Phosphate oxygen DNA polymerase-like mechanismArg/Lys cluster + potassium ion bound to Ser/Thr/Asn/Asp + magnesium bound to acidic residues + magnesium bound to substrate/intermediate
  • T00043; Pyruvate kinase I
    (Catalytic domains: 2.40.33.10, 3.20.20.60)
  • 3.133.90010.334Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Adenylate-kinase-like mechanismArg/Lys cluster + mainchain amide + magnesium ion bound to acidic residues
  • S00465; Glucose-1-phosphate thymidylyltransferase 1
    (Catalytic domain: 3.90.550.10)
  • 3.133.90010.373Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Adenylate-kinase-like mechanismHis/Lys cluster + magnesium ion bound to acidic residue
  • S00318; Glycerol-3-phosphate cytidylyltransferase
    (Catalytic domain: 3.40.50.620)
  • 3.133.90010.389Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Adenylate-kinase-like mechanismLys/Arg/His cluster + magnesium ion bound to an acidic residue
  • S00548; Adenylate kinase
    (Catalytic domain: 3.40.50.300)
  • 3.133.90010.393Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Adenylate-kinase-like mechanismLys/Arg + magnesium ion bound to Asp/Asn
  • D00417; Bifunctional protein GlmU
    (Catalytic domains: 2.160.10.10, 3.90.550.10)
  • 3.133.90010.394Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Adenylate-kinase-like mechanismLys/Arg cluster + magnesium ion bound to an acidic residue
  • S00305; Adenylate kinase
    (Catalytic domain: 3.40.50.300)
  • S00547; Adenylate kinase
    (Catalytic domain: 3.40.50.300)
  • 3.133.90030.334Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Thymidylate-kinase-like mechanismArg/Lys cluster + mainchain amide + magnesium ion bound to acidic residues
  • S00709; Glucose-1-phosphate cytidylyltransferase
    (Catalytic domain: 3.90.550.10)
  • 3.133.90030.336Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Thymidylate-kinase-like mechanismArg/His cluster + mainchain amide + magnesium ion bound
  • S00549; Nicotinamide-nucleotide adenylyltransferase
    (Catalytic domain: 3.40.50.620)
  • 3.133.90030.381Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Thymidylate-kinase-like mechanismHis/Lys/Arg cluster + mainchain amide + magnesium ion bound
  • S00316; Nicotinamide mononucleotide adenylyltransferase 1
    (Catalytic domain: 3.40.50.620)
  • 3.133.90030.383Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Thymidylate-kinase-like mechanismHis/Lys/Arg cluster + magnesium ion bound
  • S00317; Phosphopantetheine adenylyltransferase
    (Catalytic domain: 3.40.50.620)
  • 3.133.90030.392Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Thymidylate-kinase-like mechanismLys/Arg cluster + magnesium ion bound
  • S00308; Cytidylate kinase
    (Catalytic domain: 3.40.50.300)
  • S00550; Cytidylate kinase
    (Catalytic domain: 3.40.50.300)
  • 3.133.90030.394Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Thymidylate-kinase-like mechanismLys/Arg cluster + magnesium ion bound to an acidic residue
  • D00130; Deoxynucleotide monophosphate kinase
    (Catalytic domain: 3.40.50.300)
  • S00527; Thymidylate kinase
    (Catalytic domain: 3.40.50.300)
  • 3.133.90030.395Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Thymidylate-kinase-like mechanismLys/Arg/Tyr cluster + magnesium ion bound to acidic residue
  • D00129; Guanylate kinase
    (Catalytic domains: 3.30.63.10, 3.40.50.300)
  • D00540; Guanylate kinase
    (Catalytic domains: 3.30.63.10, 3.40.50.300)
  • 3.133.90030.398Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen Thymidylate-kinase-like mechanismLys/Arg cluster + magnesium ion bound to Ser or Thr
  • S00306; Thymidylate kinase
    (Catalytic domain: 3.40.50.300)
  • 3.133.91040.388Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen HMP kinase 2nd reaction step-like mechanismLys + mainchain amide groups + magnesium ion bound to Thr
  • S00705; Phosphomethylpyrimidine kinase
    (Catalytic domain: 3.40.1190.20)
  • 3.133.300000.396Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen mRNA capping enzyme-like mechanismLys/Asp + Arg/Lys/Asn/Asp cluster + magnesium or manganese ion bound
  • T00050; mRNA-capping enzyme
    (Catalytic domains: 2.40.50.140, 3.90.63.10, 4.10.87.10)
  • 3.133.305000.384Transfer Phosphate group from Phosphate oxygen to Phosphate oxygen DNA topoisomerase II autotransfer mechanismHis/Glu + Arg/His/Gln cluster + mainchain amide + magnesium ion bound
  • D00302; RNA 3''-terminal phosphate cyclase
    (Catalytic domain: 3.65.10.20)
  • 3.203.800.83Transfer Sulfonic acid group from Phosphate oxygen to Hydroxyl OxygenAlcohol sulfotransferase-like mechanismHis/Lys/Ser
  • S00309; Bile salt sulfotransferase
    (Catalytic domain: 3.40.50.300)
  • S00310; Estrogen sulfotransferase
    (Catalytic domain: 3.40.50.300)
  • 3.676.249900.37Transfer Peroxide oxygen from peroxide oxygen to sulfur atom1-Cys-Peroxiredoxin 1st reaction step-like mechanismCys + Arg + [Thr or Ser] + mainchain amide groups
  • D00869; Hybrid peroxiredoxin hyPrx5
    (Catalytic domain: 3.40.30.10)
  • D00870; 1-Cys peroxiredoxin
    (Catalytic domain: 3.40.30.10)
  • S00916; Putative peroxiredoxin Rv2238c/MT2298
    (Catalytic domain: 3.40.30.10)
  • 3.707.20000.110Transfer Methyl/Alkyl group from Sulfur atom to Hydroxyl oxygenCap specific mRNA (nucleoside-2'-O-)-methyltransferase-like mechanismLys cluster
  • S00412; Cap-specific mRNA (nucleoside-2''-O-)-methyltransferase
    (Catalytic domain: 3.40.50.150)
  • 3.707.90500.391Transfer Methyl/Alkyl group from Sulfur atom to Hydroxyl oxygenCatechol O-methyltransferase-like mechanismLys + magnesium ion bound to acidic residues/acceptor
  • S00291; Catechol O-methyltransferase
    (Catalytic domain: 3.40.50.150)
  • 3.717.19970.25Transfer Methyl/Alkyl group from Sulfur atom to Carboxyl oxygenProtein-repair-methyltransferase-like mechanismSer
  • D00823; Protein-L-isoaspartate O-methyltransferase
    (Catalytic domain: 3.40.50.150)
  • S00639; Protein-L-isoaspartate O-methyltransferase
    (Catalytic domain: 3.40.50.150)
  • 3.747.6300.267Transfer Methyl/Alkyl group from Sulfur atom to Amine nitrogen (sp3)Modification methylase RsrI-like mechanismionized Asp or Glu
  • D00079; Modification methylase DpnIIA
    (Catalytic domain: 3.40.50.150)
  • S00261; Modification methylase RsrI
    (Catalytic domain: 3.40.50.150)
  • 3.747.6310.11Transfer Methyl/Alkyl group from Sulfur atom to Amine nitrogen (sp3)Modification methylase-PvuII-like mechanismSer/Asp
  • S00262; Modification methylase PvuII
    (Catalytic domain: 3.40.50.150)
  • 3.747.29000.21Transfer Methyl/Alkyl group from Sulfur atom to Amine nitrogen (sp3)rRNA adenine N-6-methyltransferase-like mechanismAsn/mainchain carbonyl + aromatic residues
  • D00076; rRNA adenine N-6-methyltransferase
    (Catalytic domain: 3.40.50.150)
  • D00080; Modification methylase TaqI
    (Catalytic domain: 3.40.50.150)
  • 3.748.90280.5472Transfer Methyl/Alkyl group from Carbon (sp3) atom to Amine nitrogen (sp3)Serine hydroxymethyltransferase-like mechanismTwo acidic residues + PLP double-bonded to substrate/intermediate
  • D00085; Serine hydroxymethyltransferase
    (Catalytic domain: 3.40.640.10)
  • 3.770.220000.47Transfer Methyl/Alkyl group from Hydroxyl oxygen to Sulfur atom6-O-methylguanine-DNA methyltransferase-like mechanismCys/His/Tyr/Glu/Asn
  • D00077; Methylated-DNA--protein-cysteine methyltransferase
    (Catalytic domain: 1.10.10.10)
  • D00517; Methylated-DNA--protein-cysteine methyltransferase
    (Catalytic domain: 1.10.10.10)
  • 3.797.810.4110Transfer Methyl/Alkyl group from Sulfur atom to Carbon (double-bonded; sp2)Modification methylase-HaeIII methyl transfer mechanismGlu/Arg + mainchain carbonyl + Cys covalently bound to substrate/intermediate
  • D00082; Modification methylase HaeIII
    (Catalytic domain: 3.40.50.150)
  • 3.900.45300.71Transfer Glycosyl group from Hydroxyl oxygen to Hydroxyl oxygenAnhydrosialidase-like mechanismAsp/Glu/Tyr
  • T00064; Anhydrosialidase
    (Catalytic domain: 2.120.10.10)
  • 3.900.275800.990Transfer Glycosyl group from Hydroxyl oxygen to Hydroxyl oxygenCyclodextrin-glycosyltransferase-like mechanismThree acidic residues + His
  • M00113; Cyclomaltodextrin glucanotransferase
    (Catalytic domain: 3.20.20.80)
  • S00202; 4-alpha-glucanotransferase
    (Catalytic domain: 3.20.20.80)
  • T00307; 1,4-alpha-glucan branching enzyme GlgB
    (Catalytic domain: 3.20.20.80)
  • 3.903.70210.354Transfer Glycosyl group from Phoshate oxygen to Hydroxyl oxygenDNA beta-glucosyltransferase-like mechanismAsp + magnesium ion bound to Asp
  • D00403; DNA beta-glucosyltransferase
    (Catalytic domain: 3.40.50.2000)
  • 3.903.70210.640Transfer Glycosyl group from Phoshate oxygen to Hydroxyl oxygenDNA beta-glucosyltransferase-like mechanismAn acidic residue + manganese ion bound to an acidic residue
  • T00415; Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
    (Catalytic domain: 3.90.550.10)
  • 3.903.137500.660Transfer Glycosyl group from Phoshate oxygen to Hydroxyl oxygenEXTL2-acetylhexosaminyltransferase-like mechanismArg + mainchain amide + Asn/Asp + manganese ion bound to an acidic residue
  • D00860; Exostosin-like 2
    (Catalytic domain: 3.90.550.10)
  • 3.940.275890.113Transfer Glycosyl group from Hydroxyl oxygen to Amine nitrogen (sp3)Endonuclease III glycosyl transfer mechanismLys + two acidic residues
  • D00266; Endonuclease III
    (Catalytic domains: 1.10.340.30, 1.10.1670.10)
  • 3.943.120000.356Transfer Glycosyl group from Phosphate oxygen to Amine nitrogen (sp3)Uracil phosphoribosyltransferase-like mechanismAsp/Asp/Asp + magnesium ion bound
  • S00287; Uracil phosphoribosyltransferase
    (Catalytic domain: 3.40.50.2020)
  • 3.943.131000.356Transfer Glycosyl group from Phosphate oxygen to Amine nitrogen (sp3)Xanthine phosphoribosyltransferase-like mechanismAsp/Asp/Asp + magnesium ion bound
  • S00289; Xanthine phosphoribosyltransferase
    (Catalytic domain: 3.40.50.2020)
  • 3.944.220700.2Transfer Glycosyl group from Amine nitrogen (sp3) to Amine nitrogen (sp3)TGT-like mechanismTwo acidic residues
  • S00230; Queuine tRNA-ribosyltransferase
    (Catalytic domain: 3.20.20.105)
  • 3.944.290000.2Transfer Glycosyl group from Amine nitrogen (sp3) to Amine nitrogen (sp3)Nucleoside deoxyribosyltransferase-like mechanismTwo acidic residues
  • S00377; Nucleoside deoxyribosyltransferase
    (Catalytic domain: 3.40.50.1810)
  • 3.955.50030.967Transfer Glycosyl group from Imine nitrogen (sp2) to Imine nitrogen (sp2)NAD+ ADP-ribosyltransferase-like mechanismTwo acidic residues + Arg cluster
  • D00463; Vip2Ac
    (Catalytic domain: 3.90.176.10)
  • 3.1103.6090.130Transfer Acyl group from Phosphate oxygen to Hydroxyl oxygenGlutaminyl-tRNA synthetase acyl transfer mechanismAn acidic residue + Lys/His/Arg + mainchain amide
  • M00178; Glutaminyl-tRNA synthetase
    (Catalytic domains: 1.10.1160.10, 3.40.50.620)
  • 3.1107.6300.74Transfer Acyl group from Sulfur atom to Hydroxyl oxygenModification methylase RsrI-like mechanismHis
  • D00464; Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
    (Catalytic domain: 2.160.10.10)
  • 3.1107.220190.41Transfer Acyl group from Sulfur atom to Hydroxyl oxygenArylamine N-acetyltransferase-like mechanismCys/His/Asp + mainchain amide
  • D00508; N-hydroxyarylamine O-acetyltransferase
    (Catalytic domain: 2.40.128.40)
  • 3.1143.50000.64Transfer Acyl group from Phosphate oxygen to Amine nitrogen (sp3)Aspartate--ammonia ligase acyl transfer mechanismAsp/Arg/Gln
  • S00413; Aspartate--ammonia ligase
    (Catalytic domain: 3.30.930.10)
  • 3.1143.70030.3060Transfer Acyl group from Phosphate oxygen to Amine nitrogen (sp3)NH(3)-dependent NAD+ synthetase acyl transfer mechanismAsp + monovalent cation bound
  • S00315; NH(3)-dependent NAD(+) synthetase
    (Catalytic domain: 3.40.50.620)
  • 3.1143.80000.1190Transfer Acyl group from Phosphate oxygen to Amine nitrogen (sp3)Folylpolyglutamate synthase acyl transfer mechanismHis + two magnesium ions bound
  • D00516; Folylpolyglutamate synthase
    (Catalytic domain: 3.40.1190.10)
  • T00109; UDP-N-acetylmuramoylalanine--D-glutamate ligase
    (Catalytic domain: 3.40.1190.10)
  • 3.1143.90000.1132Transfer Acyl group from Phosphate oxygen to Amine nitrogen (sp3)Gro-PCT-like mechanismArg cluster + mainchain amide + two magnesium ions bound to acidic residues/Asn/Gln
  • M00051; Glutathione synthetase
    (Catalytic domains: 1.10.1080.10, 3.30.470.20, 3.30.1490.50)
  • T00107; Glutathione synthetase
    (Catalytic domains: 3.30.470.20, 3.30.1490.20)
  • 3.1144.1800.89Transfer Acyl group from Amine nitrogen (sp3) to Amine nitrogen (sp3)Methionyl-tRNA formyltransferase-like mechanismHis/Asn/Asp
  • D00087; Methionyl-tRNA formyltransferase
    (Catalytic domain: 3.40.50.170)
  • S00294; Phosphoribosylglycinamide formyltransferase
    (Catalytic domain: 3.40.50.170)
  • 3.1144.220195.34Transfer Acyl group from Amine nitrogen (sp3) to Amine nitrogen (sp3)Cofactor-dependent-phosphoglycerate-mutase-like mechanismThr(N-terminal) + N-terminal alpha-amine + Thr + mainchain amide
  • T00418; Gamma-glutamyltranspeptidase
    (Catalytic domain: 3.60.20.10)
  • 3.1147.900.95Transfer Acyl group from Sulfur atom to Amine nitrogen (sp3)Serotonin N-acetyltransferase-like mechanismTyr/His/His + mainchain amide
  • S00410; Serotonin N-acetyltransferase
    (Catalytic domain: 3.40.630.30)
  • 3.1147.6000.72Transfer Acyl group from Sulfur atom to Amine nitrogen (sp3)Histone acetyltransferase type B-like mechanismGlu/hydrophobic residues + mainchain amide
  • M00165; General control of amino acid synthesis protein 5-like 2
    (Catalytic domain: 3.40.630.30)
  • T00034; Histone acetyltransferase type B catalytic subunit
    (Catalytic domain: 3.40.630.30)
  • 3.1147.6010.88Transfer Acyl group from Sulfur atom to Amine nitrogen (sp3)Glucosamine-1-phosphate N-acetyltransferase-like mechanismHis/Glu/Ser + mainchain amide
  • D00417; Bifunctional protein GlmU
    (Catalytic domains: 2.160.10.10, 3.90.550.10)
  • 3.1147.37500.97Transfer Acyl group from Sulfur atom to Amine nitrogen (sp3)Histone acetyltransferase HPA2-like mechanismTyr + Tyr/mainchain amide + Hydrophobic residues/mainchain carbonyl
  • S00409; Histone acetyltransferase HPA2
    (Catalytic domain: 3.40.630.30)
  • 3.1147.47600.180Transfer Acyl group from Sulfur atom to Amine nitrogen (sp3)NMT-like mechanismC-terminal carboxylate + Asn + mainchain amide groups + Thr or Ser + mainchain carbonyl
  • D00413; Glycylpeptide N-tetradecanoyltransferase
    (Catalytic domain: 3.40.630.30)
  • 3.1177.805.79Transfer Acyl group from Sulfur atom to Sulfur atomDihydrolipoamide-succinyltransferase-like mechanismHis/Asn + mainchain carbonyl + a residue with hydroxyl group [Ser or Thr]
  • M00189; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
    (Catalytic domain: 3.30.559.10)
  • 3.1177.805.87Transfer Acyl group from Sulfur atom to Sulfur atomDihydrolipoamide-succinyltransferase-like mechanismHis/Asp/Arg + mainchain carbonyl + a residue with hydroxyl group [Ser or Thr]
  • M00188; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
    (Catalytic domain: 3.30.559.10)
  • M00190; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
    (Catalytic domain: 3.30.559.10)
  • T00223; Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
    (Catalytic domain: 3.30.559.10)
  • 3.1177.220080.38Transfer Acyl group from Sulfur atom to Sulfur atomBeta-ketoacyl-ACP synthase III 1st reaction step-like mechanismCys + an acidic residue + [Ser or Thr] + His/Asn + mainchain amide groups
  • D00825; 3-oxoacyl-[acyl-carrier-protein] synthase 3
    (Catalytic domain: 3.40.47.10)
  • D00826; 3-oxoacyl-[acyl-carrier-protein] synthase 3
    (Catalytic domain: 3.40.47.10)
  • D00867; 3-oxoacyl-[acyl-carrier-protein] synthase 3
    (Catalytic domain: 3.40.47.10)
  • 3.1177.220085.58Transfer Acyl group from Sulfur atom to Sulfur atomBeta-ketoacyl-ACP synthase II 1st reaction step-like mechanismCys + His/His/Lys + mainchain amide groups
  • D00871; 3-oxoacyl-[acyl-carrier-protein] synthase 2
    (Catalytic domain: 3.40.47.10)
  • 3.1187.65790.5550Transfer Acyl group from Sulfur atom to Carbon (sp3)glycine acetyltransferase mechanismLys/Ser + PLP double-bonded to substrate/intermediate
  • D00639; 2-amino-3-ketobutyrate coenzyme A ligase
    (Catalytic domain: 3.40.640.10)
  • 3.1187.70500.5510Transfer Acyl group from Sulfur atom to Carbon (sp3)8-amino-7-oxononanoate synthase acyl transfer mechanismLys/His + PLP double-bonded to substrate/intermediate
  • D00092; 8-amino-7-oxononanoate synthase
    (Catalytic domains: 3.40.640.10, 3.90.1150.10)
  • 3.1197.15020.136Transfer Acyl group from Sulfur atom to Carbon (double-bonded; sp2)Beta-ketoacyl-ACP synthase III 3rd reaction step-like mechanismAn acidic residue + [Ser or Thr] + mainchain amide groups + His + [Asn or Gln] + Cys covalently-bound to substrate/intermediate
  • D00825; 3-oxoacyl-[acyl-carrier-protein] synthase 3
    (Catalytic domain: 3.40.47.10)
  • D00826; 3-oxoacyl-[acyl-carrier-protein] synthase 3
    (Catalytic domain: 3.40.47.10)
  • D00867; 3-oxoacyl-[acyl-carrier-protein] synthase 3
    (Catalytic domain: 3.40.47.10)
  • 3.1197.15025.150Transfer Acyl group from Sulfur atom to Carbon (double-bonded; sp2)Beta-ketoacyl-ACP synthase II 3rd reaction step-like mechanismHis + mainchain amide groups + Cys covalently-bound to substrate/intermediate
  • D00871; 3-oxoacyl-[acyl-carrier-protein] synthase 2
    (Catalytic domain: 3.40.47.10)
  • 3.1244.220200.43Transfer Amidino group from Amine nitrogen (sp3) to Amine nitrogen (sp3)Glycine amidinotransferase-like mechanismCys/His/Asp/Asp + acidic residue
  • S00440; Glycine amidinotransferase
    (Catalytic domain: 3.75.10.10)
  • 4.12.18410.92Addition to double-bond Water (H2O; hydration) to Imine carbon (sp2) [leading to hydorxyl group & amine group]Creatinase-like addition mechanismHis + two acidic residues
  • D00244; Creatinase
    (Catalytic domain: 3.90.230.10)
  • 4.12.642300.465Addition to double-bond Water (H2O; hydration) to Imine carbon (sp2) [leading to hydorxyl group & amine group]Base-Acid/Stabilizer; Base activation of added group; Inorganic-cofactor-assisted added group attacks on addition site; Simultaneous acid protonation to protonation site; Stabilization of group adjacent to addition site mechanismGlu + mainchain carbonyl + zinc ion bound to Cys/His cluster
  • D00406; Cytidine deaminase
    (Catalytic domain: 3.40.140.10)
  • S00808; Deoxycytidylate deaminase
    (Catalytic domain: 3.40.140.10)
  • S00810; Guanine deaminase
    (Catalytic domain: 3.40.140.10)
  • 4.12.642320.458Addition to double-bond Water (H2O; hydration) to Imine carbon (sp2) [leading to hydorxyl group & amine group]Adenosine deaminase 1st reaction step-like mechanismAsp/Glu/His + zinc ion bound to His/(acidic residue) cluster
  • D00873; Guanine deaminase
    (Catalytic domain: 3.20.20.140)
  • S00232; Adenosine deaminase
    (Catalytic domain: 3.20.20.140)
  • 4.12.642320.562Addition to double-bond Water (H2O; hydration) to Imine carbon (sp2) [leading to hydorxyl group & amine group]Adenosine deaminase 1st reaction step-like mechanismAsp/Glu/His + Fe(II) ion bound to His/(acidic residue) cluster
  • D00675; Cytosine deaminase
    (Catalytic domain: 3.20.20.140)
  • 4.15.898500.454Addition to double-bond Water (H2O; hydration) to CO2 [leading to hydorxyl group & carboxylate; fixation of CO2]Base-stabilizer/Stabilizer; Inorganic-cofactor-assisted activation of added group by base; Added group stabilized; Addition-site stabilized; Activated added group attacks on the addition-site mechanismAsp/Gly + zinc ion bound to Cys/Cys/His cluster
  • S00521; Carbonic anhydrase
    (Catalytic domain: 3.40.1050.10)
  • 4.15.898510.455Addition to double-bond Water (H2O; hydration) to CO2 [leading to hydorxyl group & carboxylate; fixation of CO2]Base-stabilizer/Stabilizer/Proton-shuttle; Inorganic-cofactor-assisted base activation of added group through proton-shuttle; Added group stabilized; Addition-site of unsaturated group stabilized; Activated added group attacks on the addition-site mechanismAsp/Gln/Tyr + zinc ion bound to Cys/Cys/His cluster
  • D00474; Carbonic anhydrase
    (Catalytic domain: 3.40.1050.10)
  • 4.15.898520.455Addition to double-bond Water (H2O; hydration) to CO2 [leading to hydorxyl group & carboxylate; fixation of CO2]Base-proton-shuttle-stabilizer/Stabilizer/proton-shuttle; Inorganic-cofactor-assisted activation of added group by base-proton-shuttle; Added group stabilized; Addition-site of unsaturated group stabilized; Activated added group attacks on the addition-site of unsaturated group mechanismAsp/Gln/Tyr + zinc ion bound to Cys/Cys/His cluster
  • S00424; Carbonic anhydrase
    (Catalytic domain: 3.40.1050.10)
  • 4.15.1107000.480Addition to double-bond Water (H2O; hydration) to CO2 [leading to hydorxyl group & carboxylate; fixation of CO2]Base-stabilizer/Proton-shuttle/Stabilizer; Inorganic-cofactor-assisted base deprotonation of added group through proton shuttle; Addition-site of unsaturated group stabilized; Activated added group attacks on the addition-site mechanismTwo acidic residues + Arg/Gln/Asn + zinc ion bound to His cluster
  • S00167; Carbonic anhydrase
    (Catalytic domain: 2.160.10.10)
  • 4.16.66400.4Addition to double-bond Water (H2O; hydration) to sp2-like Carbon (C=O-PO3) [leading to HO-C-OPO3]Base/Base; Base deprotonation of added group; Activated added group attacks on addition site of unsaturated group; 2nd base deprotonation of protonated 1st base mechanismAn acidic residue + His
  • S00532; 2-dehydro-3-deoxyphosphooctonate aldolase
    (Catalytic domain: 3.20.20.70)
  • 4.16.66400.7Addition to double-bond Water (H2O; hydration) to sp2-like Carbon (C=O-PO3) [leading to HO-C-OPO3]Base/Base; Base deprotonation of added group; Activated added group attacks on addition site of unsaturated group; 2nd base deprotonation of protonated 1st base mechanismAn acidic residue + Lys
  • S00243; Phospho-2-dehydro-3-deoxyheptonate aldolase
    (Catalytic domain: 3.20.20.70)
  • 4.23.18400.69Addition to double-bond Hydroxyl oxygen to Carbon (sp2) (with carboxylate group)UDP-N-acetylglucosamine 1-carboxyvinyltransferase-like addition mechanismAsp/Cys/Asn
  • D00415; UDP-N-acetylglucosamine 1-carboxyvinyltransferase
    (Catalytic domain: 3.65.10.10)
  • 4.121.143000.6100Addition to double-bond Carbon (sp3) (adjacent to double-bonded sp2 carbon ) to Carbonyl carbon (sp2)Acid; Added group attacks on addition site of unsaturated group to form covalent bond; Acid protonation to protonation site of unsaturated group mechanismAn acidic residue + Lys double-bonded to substrate/intermediate
  • S00198; Transaldolase B
    (Catalytic domain: 3.20.20.70)
  • 4.151.766500.451Addition to double-bond Carbon (sp2) (C=C-O) to Carbonyl carbon (sp2)Modulator-Acid/Stabilizer; Inorganic-cofactor-assisted stabilization of added group of substrate; Modulator-assisted activation of electrophilic unsaturated group; Added group attacks on unsaturated group forming covalent bond; Acid protonation to protonation site of unsaturated group mechanismAn acidic residue + Asn (or Gln) + zinc ion bound to His cluster
  • S00235; Fructose-bisphosphate aldolase class 2
    (Catalytic domain: 3.20.20.70)
  • 4.151.774000.401Addition to double-bond Carbon (sp2) (C=C-O) to Carbonyl carbon (sp2)Acid; Inorganic-cofactor-assisted activation of protonation site of unsaturated group; Added group attacks on addition site of unsaturated group; Acid protonation to protonation site of unsaturated group mechanismLys + a divalent metal bound to substrate/Cys/His/Acidic residues
  • S00243; Phospho-2-dehydro-3-deoxyheptonate aldolase
    (Catalytic domain: 3.20.20.70)
  • S00532; 2-dehydro-3-deoxyphosphooctonate aldolase
    (Catalytic domain: 3.20.20.70)
  • 4.162.20000.1Addition to double-bond Carbon (sp2) (C=C-N) to Imine carbon (sp2)Base-Acid; Base activation of added group; Nucleophilic attack on addition site of unsaturated group by added group; substrate-assisted base deprotonates addition site of added group through water; Acid protonation to 2-bonds away from addition site of added group mechanismAn acidic resdiue
  • S00276; Deoxycytidylate 5-hydroxymethyltransferase
    (Catalytic domain: 3.30.572.10)
  • 4.191.350400.117Addition to double-bond Aromatic carbon (sp2) (C=C-N) to Carbonyl carbon (sp2) [leading to hydorxyl group & imine group]Acid/Modulator-Stabilizer/Stabilizer/Modulator; Acid protonation to protonation site of unsaturated group; Electrophilic addition site attacks on added group; Modulation of addition site and added group through other site; Modulator-asisted reprotonation of acid mechanismLys/Lys + two acidic residues
  • S00826; Indole-3-glycerol phosphate synthase
    (Catalytic domain: 3.20.20.70)
  • 4.202.112900.6000Addition to double-bond Amine group (sp3 nitrogen) to Imine carbon (sp2)Substrate-assisted-Base-Acid; Substrate-base activation of added group; Activated added group attacks on addition site of unsaturated group; Substrate-acid protonation to protonation site of unsaturated group mechanismLys double-bonded to substrate/intermediate
  • S00239; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00538; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • 4.202.3822800.1Addition to double-bond Amine group (sp3 nitrogen) to Imine carbon (sp2)Base; Base deprotonation of added group; Activated added group attacks on the addition-site mechanismAn acidic resdiue
  • D00085; Serine hydroxymethyltransferase
    (Catalytic domain: 3.40.640.10)
  • 4.501.3813400.59Addition to double-bond Sulfur to Carbonyl carbon (sp2)Betaine aldehyde dehydrogenase 1st reaction step-like mechanismCys + an acidic residue + [Asn or Gln] + mainchain amide group
  • D00021; Betaine aldehyde dehydrogenase
    (Catalytic domains: 3.40.309.10, 3.40.605.10)
  • 4.501.3944060.57Addition to double-bond Sulfur to Carbonyl carbon (sp2)GDP mannose dehydrogenase 2nd reaction step-like mechanismCys/Lys + Lys/Asp/Thr
  • T00227; UDP-glucose 6-dehydrogenase
    (Catalytic domains: 1.10.1040.10, 3.40.50.720)
  • T00408; GDP-mannose 6-dehydrogenase
    (Catalytic domains: 1.-.-.-, 3.40.50.720)
  • 4.504.771000.5521Addition to double-bond Sulfur to Carbon (sp2) adjacent to double-bondAcid/Modulator; Added group attacks on addition site of unsaturated group; Organic-cofactor bound to unsaturated group stabilizes intermediate; Modulator-assisted acid protonation to protonation site of unsaturated group mechanismLys/Tyr + PLP covalently bound to substrate/intermediate
  • D00265; Cystathionine gamma-synthase
    (Catalytic domain: 3.40.640.10)
  • 4.1010.695600.8301Addition to double-bond Water (H2O; hydration) to Carbon (sp2) adjacent to double-bond; Opposition to non-beta-elimination6-phospho-alpha-glucosidase 4th reaction step-like mechanismAn acidic residue + manganese ion bound to Cys/His/O7-nicotinamide/substrate or intermediate
  • D00827; Maltose-6''-phosphate glucosidase
    (Catalytic domain: 3.90.110.10)
  • 4.1034.143290.135Addition to double-bond Carboxylate oxygen to Carbon (sp2) adjacent to double-bond; Opposition to non-beta-eliminationMetal-independent eukaryotic MLE-like mechanismAn acidic residue + Arg cluster
  • S00852; Carboxy-cis,cis-muconate cyclase
    (Catalytic domain: 2.130.10.10)
  • 4.1034.769660.650Addition to double-bond Carboxylate oxygen to Carbon (sp2) adjacent to double-bond; Opposition to non-beta-eliminationMetal-dependent bacterial MLE-like mechanismAn acidic residue + Lys + manganese ion bound to acidic residues
  • D00282; Muconate cycloisomerase 1
    (Catalytic domain: 3.20.20.120)
  • D00283; Chloromuconate cycloisomerase
    (Catalytic domain: 3.20.20.120)
  • 4.1164.693000.5470Addition to double-bond Carbon (sp2) (C=C-N) to Carbon (sp2) adjacent to double-bond; Opposition to non-beta-eliminationBase; Added group attacks on addition site of unsaturated group; Organic-cofactor bound to unsaturated group stabilizes intermediate; Base deprotonation of addition site of added group mechanismAn acidic residue + PLP double-bonded to substrate/intermediate
  • T00089; Tryptophan synthase alpha chain
    (Catalytic domains: 3.20.20.70, 3.40.50.1100)
  • 4.1244.165000.313Addition to double-bond Nitrogen atom in aromatic ring (sp2 nitrogen group) to Carbon (sp2) adjacent to double-bond; Opposition to non-beta-eliminationModulator/Stabilizer; Stabilization of protonation site of unsaturated group & Modulation of addition site of unsaturated group and substrate-assisted acid; Inorganic-cofactor-asisted stabilization of substrate-assisted acid; Added group attacks on unsaturated group & Substrate-assisted acid protonation to protonation site of unsaturated group mechanismSer/Gln + magnesium ion bound to acidic residues/phosphate
  • S00220; Thiamine-phosphate pyrophosphorylase
    (Catalytic domain: 3.20.20.70)
  • 4.1504.3900000.53Addition to double-bond Sulfur to Carbon (sp2) adjacent to double-bond; Opposition to non-beta-eliminationNucleophile/Acid; Nucleophile attacks on addition site of unsaturated group; Acid protonates protonation site of unsaturated group mechanismCys + an acidic residue
  • S00276; Deoxycytidylate 5-hydroxymethyltransferase
    (Catalytic domain: 3.30.572.10)
  • 4.1514.818600.1130Addition to double-bond Sulfur to Carbon (sp2) adjacent to double-bond; Opposition to non-beta-eliminationStabilizer; Stabilization of protonation site of unsaturated group by inorganic cofactor; Stabilized added group attacks on addition site of unsaturated group mechanismArg cluster + two magnesium ions bound to acidic residues
  • S00841; Phosphosulfolactate synthase
    (Catalytic domain: 3.20.20.70)
  • 5.10.9510.969Eliminative double-bond formation Hydroxyl group (dehydration) from Carbon (sp3)dTDP glucose dehydratase 2nd reaction step-like mechanismTwo acidic residues + Tyr/Thr
  • D00262; dTDP-glucose 4,6-dehydratase 2
    (Catalytic domain: 3.40.50.720)
  • 5.10.12500.6010Eliminative double-bond formation Hydroxyl group (dehydration) from Carbon (sp3)Base-Acid/Modulator; Base deprotonates deprotonation site; Intermediate formation stabilized by tautomerization; Acid protonates eliminated group leading to bond cleavage; E1Cb-like reaction mechanismHis/Glu + Lys double-bonded to substrate/intermediate
  • S00240; 3-dehydroquinate dehydratase
    (Catalytic domain: 3.20.20.70)
  • 5.10.68000.77Eliminative double-bond formation Hydroxyl group (dehydration) from Carbon (sp3)Base-Acid/Stabilizer/Modulators; Base deprotonates deprotonation site; Intermediate formation stabilized; Modulator-activated water protonates leaving group (non-deprotonation/elimination site) & Acid protonation to eliminated group leading to bond cleavage; Water deprotonates leaving group (non-deprotonation/elimination site) ; E1cb-like reaction mechanismHis + His+ Asp + Tyr cluster
  • S00176; Scytalone dehydratase
    (Catalytic domain: 3.10.450.50)
  • 5.10.552200.114Eliminative double-bond formation Hydroxyl group (dehydration) from Carbon (sp3)Base/Acid; Acid protonation to eliminated group; Base deprotonation of dedeprotonation site leading to bond cleavage; E2-like reaction mechanismLys/Glu
  • S00826; Indole-3-glycerol phosphate synthase
    (Catalytic domain: 3.20.20.70)
  • 5.12.1132000.75Eliminative double-bond formation Hydroxyl group (dehydration) from Carbon (sp3) (with amine group) [leading to imine group]Base-Acid/Acid/Modulator/Stabilizer; Modulator-activated base deprotonates leaving group (non-deprotonation/elimination site); Intermediate formation stabilized; Acid protonation to eliminated group leading to bond cleavage; Water deprotonates deprotonation site of leaving group; 2nd Acid protonates leaving group (non-deprotonation/elimination site); E1-like reaction mechanismHis/His + acidic residues + mainchain amide
  • S00254; Pterin-4-alpha-carbinolamine dehydratase
    (Catalytic domain: 3.30.1360.20)
  • 5.12.1497400.1Eliminative double-bond formation Hydroxyl group (dehydration) from Carbon (sp3) (with amine group) [leading to imine group]Acid; Acid protonation to eliminated group; E1-like reaction mechanismAn acidic resdiue
  • S00226; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
    (Catalytic domain: 3.20.20.70)
  • S00847; N-(5''-phosphoribosyl)anthranilate isomerase
    (Catalytic domain: 3.20.20.70)
  • 5.12.1504210.1Eliminative double-bond formation Hydroxyl group (dehydration) from Carbon (sp3) (with amine group) [leading to imine group]Acid; Acid protonation to eliminated group completes reaction; E1-like reaction mechanismAn acidic resdiue
  • D00085; Serine hydroxymethyltransferase
    (Catalytic domain: 3.40.640.10)
  • 5.14.411700.1Eliminative double-bond formation Hydroxyl group (dehydration) from Carbon (sp3) adjacent to double-bondGDP mannose dehydratase 3rd reaction step-like mechanismAn acidic residue
  • D00513; GDP-mannose 4,6 dehydratase 2
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • D00543; GDP-mannose 4,6-dehydratase
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • 5.14.3200000.372Eliminative double-bond formation Hydroxyl group (dehydration) from Carbon (sp3) adjacent to double-bondAcid/Stabilizer; Acid protonation to eliminated group leading to bond cleavage; Inorganic cofactor-assisted stabilization of intermediate; Inorganic cofactor contribution to leaving group (non-deprotonation/elimination site); E1-like reaction mechanismHis + Tyr/Lys/Asn + magnesium ion bound to Asp/Glu/Asn
  • D00261; Glucarate dehydratase
    (Catalytic domain: 3.20.20.120)
  • 5.14.3250000.5471Eliminative double-bond formation Hydroxyl group (dehydration) from Carbon (sp3) adjacent to double-bondAcid; Acid protonation to eliminated group; Organic cofactor-assisted bond cleavage; E1-like reaction mechanismAn acidic residue + PLP covalently bound to substrate/intermediate
  • T00089; Tryptophan synthase alpha chain
    (Catalytic domains: 3.20.20.70, 3.40.50.1100)
  • 5.20.8010.94Eliminative double-bond formation O-glycoside group from Carbon (sp3)Base-Acid/Stabilizer/Modulator; Modulater-activated base deprotonates deprotonation site; Intermediate formation stabilized; Acid protonates eliminated group leading to cleavage; E1Cb-like reaction mechanismTyr/His/Arg
  • D00518; Alginate lyase
    (Catalytic domain: 1.50.10.110)
  • 5.20.1641500.532Eliminative double-bond formation O-glycoside group from Carbon (sp3)Base/Acid/Stabilizer; Inorganic-cofactor-stabilization of functional group adjacent to deprotonation site leading to modulation of deprotonation site; Base deprotonation of deprotonation site of leaving group; Intermediate formation; Stabilization of eliminated group & Acid protonation to eliminated group leading to bond cleavage; E1cb-like reaction mechanismLys + an acidic residue + Arg + calcium ion bound to Asn + two acidic residues
  • S00837; Chondroitinase-B
    (Catalytic domain: 2.160.20.10)
  • 5.20.1710000.530Eliminative double-bond formation O-glycoside group from Carbon (sp3)Base/Stabilizer; Substrate-assisted modulation of base; Inorganic cofactor-assisted base deprotonation of deprotonation site; Inorganic cofactor-assisted stabilization of intermediate formation; Water protonation to eliminated group; E1cb-like reaction mechanismArg/Lys + calcium ion bound to acidic residues
  • S00169; Pectate lyase C
    (Catalytic domain: 2.160.20.10)
  • S00546; Pectate lyase
    (Catalytic domain: 2.160.20.10)
  • 5.21.14000.74Eliminative double-bond formation O-glycoside group from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Base-Acid; Base deprotonates deprotonation site; Acid protonates eliminated group leading to bond cleavage; E1Cb-like reaction mechanismHis
  • T00201; Glucosamine--fructose-6-phosphate aminotransferase {isomerizing}
    (Catalytic domains: 3.40.50.10490, 3.60.20.10)
  • 5.30.1645000.5521Eliminative double-bond formation Carboxyl ester (oxygen) from Carbon (sp3)Base/Acid; Base deprotonation of deprotonation site of leaving group; Intermediate formation; Acid protonation to eliminated group leading to bond cleavage; E1cb-like reaction; Organic-cofactor-assisted reaction mechanismLys/Tyr + PLP covalently bound to substrate/intermediate
  • D00265; Cystathionine gamma-synthase
    (Catalytic domain: 3.40.640.10)
  • 5.41.2776000.400Eliminative double-bond formation Phosphoryl oxygen (O3P-O-) from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Acid-Base/Stabilizer; Inorganic cofactor-assisted stabilization of group attached to elimination site of leaving group; Acid protonation to elimination group; Base deprotonation of deprotonation site of leaving group; Stabilization of eliminated group facilitates bond cleavage; E1-like reaction mechanismLys/Arg + mainchain amide + a divalent metal bound to Cys/His/Acidic residues
  • S00243; Phospho-2-dehydro-3-deoxyheptonate aldolase
    (Catalytic domain: 3.20.20.70)
  • S00532; 2-dehydro-3-deoxyphosphooctonate aldolase
    (Catalytic domain: 3.20.20.70)
  • 5.104.2187000.9100Eliminative double-bond formation Carbon (sp3) from Carbon (sp3) adjacent to double-bondStabilizer/Electrophile; Initial state is electrophilic anion (no need to deprotonation of deprotonation site); Electrophilic modified residue-assisted bond cleavage; Leaving group stabilized; E1cb-like reaction; mechanismmainchain amide + MIO (Ala-Ser-Gly modified residues)
  • D00267; Histidine ammonia-lyase
    (Catalytic domains: 1.10.275.10, 1.20.200.10)
  • 5.105.3195000.7370Eliminative double-bond formation Carbon (sp3) from Carboxylate (sp2) [release of CO2]Acid/Modulator; Modulator/Organic-cofactor-assisted bond cleavage; Organic cofactor-assisted stabilization of intermediate by tautomerization; Acid protonation to eliminated group; E1 reaction mechanismHis/Ser + Thiamin diphosphate bound through calcium ion to Asp/Asn/Thr
  • T00230; Benzoylformate decarboxylase
    (Catalytic domains: 3.40.50.970, 3.40.50.1220)
  • 5.111.552000.6100Eliminative double-bond formation Methylene (adjacent to double-bonded sp2 carbon ) from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Base-Acid; Base deprotonates dedeprotonation site leading to bond cleavage; formation of eliminated-group intermediate; Acid protonates eliminated group; E2-like reaction mechanismAn acidic residue + Lys double-bonded to substrate/intermediate
  • S00222; KHG/KDPG aldolase
    (Catalytic domain: 3.20.20.70)
  • 5.111.850000.6300Eliminative double-bond formation Methylene (adjacent to double-bonded sp2 carbon ) from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Modulator; Substrate-assisted base deprotonates deprotonation site of leaving group through modulator; deprotonation leading to cleavage of eliminated group and double-bond formation; E2-like reaction mechanismTyr + Lys double-bonded to substrate/intermediate
  • S00200; N-acetylneuraminate lyase
    (Catalytic domain: 3.20.20.70)
  • 5.115.3380000.410Eliminative double-bond formation Methylene (adjacent to double-bonded sp2 carbon ) from Carboxylate (sp2) [release of CO2]Stabilizer; Inorganic cofactor-assisted stabilization of eliminated group & Leaving group stabilized; Bond-cleavage occurs by E1-like reaction mechanismTyr + a divalent metal ion bound to His/Asp/Lys
  • T00084; Phosphoenolpyruvate carboxykinase {ATP}
    (Catalytic domains: 2.170.8.10, 3.40.449.10, 3.90.228.20)
  • 5.115.129300.66Eliminative double-bond formation Methylene (adjacent to double-bonded sp2 carbon ) from Carboxylate (sp2) [release of CO2]Beta-ketoacyl-ACP synthase III 2nd reaction step-like mechanismHis + [Asn or Gln]
  • D00825; 3-oxoacyl-[acyl-carrier-protein] synthase 3
    (Catalytic domain: 3.40.47.10)
  • D00826; 3-oxoacyl-[acyl-carrier-protein] synthase 3
    (Catalytic domain: 3.40.47.10)
  • D00867; 3-oxoacyl-[acyl-carrier-protein] synthase 3
    (Catalytic domain: 3.40.47.10)
  • 5.121.670200.6100Eliminative double-bond formation Carbon (sp3) (adjacent to double-bonded sp2 carbon) from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Base; Base deprotonation of deprotonation site of leaving group leading to bond cleavage; E2-like reaction; Substrate-assisted stabilization mechanismAn acidic residue + Lys double-bonded to substrate/intermediate
  • S00198; Transaldolase B
    (Catalytic domain: 3.20.20.70)
  • 5.121.671000.6111Eliminative double-bond formation Carbon (sp3) (adjacent to double-bonded sp2 carbon) from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Base/Modulator; Modulator-assisted base deprotonation of deprotonation site of leaving group leading to bond cleavage; E2-like reaction mechanismTwo acidic residues + Lys double-bonded to substrate/intermediate
  • S00199; Fructose-bisphosphate aldolase A
    (Catalytic domain: 3.20.20.70)
  • 5.125.526200.114Eliminative double-bond formation Carbon (sp3) (adjacent to double-bonded sp2 carbon) from Carboxylate (sp2) [release of CO2]Modulator/Stabilizer; Stabilization of 2-bonds away from elimination site of eliminated group; E1cb-like reaction mechanismLys/Glu
  • S00826; Indole-3-glycerol phosphate synthase
    (Catalytic domain: 3.20.20.70)
  • 5.151.2325000.451Eliminative double-bond formation Carbon (sp2) (C=C-O) from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Modulator-Acid/Stabilizer; Inorganic-cofactor-assisted stabilization of eliminated group; Modulator-assisted activation of eliminated group; Base deprotonation of deprotonation site of leaving group; Bond cleavage of eliminated group and double-bond formation by E2-like reaction mechanismAn acidic residue + Asn (or Gln) + zinc ion bound to His cluster
  • S00235; Fructose-bisphosphate aldolase class 2
    (Catalytic domain: 3.20.20.70)
  • 5.161.586000.968Eliminative double-bond formation Carbon (sp2) (C=C-N) from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Base-Acid-Stabilizer;Acid-Base;Modulator; Modulator-assisted 1st base deprotonation of eliminated group & 1st acid protonation to elimination site of eliminated group leading to transition state; 2nd base deprotonation of deprotonation site leading to bond cleavage & 2nd acid protonation to eliminated group; E2-like reaction mechanismTwo acidic residues + Thr
  • T00089; Tryptophan synthase alpha chain
    (Catalytic domains: 3.20.20.70, 3.40.50.1100)
  • 5.171.2330000.7370Eliminative double-bond formation Carbon (sp2) (C=N-X) from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Base/Modulator; Base deprotonation of deprotonation site of leaving group; Modulator/Organic-cofactor-assisted bond cleavage; E2-like reaction mechanismHis/Ser + Thiamin diphosphate bound through calcium ion to Asp/Asn/Thr
  • T00230; Benzoylformate decarboxylase
    (Catalytic domains: 3.40.50.970, 3.40.50.1220)
  • 5.191.546000.23Eliminative double-bond formation Cyanide group (CN) from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Base/Acid-Modulator/Stabilizer/Modulator; Modulator-assisted base deprotonation of deprotonation site leading to bond cleavage; Acid protonation to eliminated group; E2-like reaction mechanismSer/His/Thr/Asp
  • S00358; Hydroxynitrilase
    (Catalytic domain: 3.40.50.1820)
  • 5.200.552010.6500Eliminative double-bond formation Amine group from Carbon (sp3)Base-Acid; Base deprotonates dedeprotonation site leading to bond cleavage & Acid protonates eliminated group; E2-like reaction mechanismLys covalently bound to substrate/intermediate
  • S00239; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00538; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • 5.201.672500.74Eliminative double-bond formation Amine group from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Creatinase-like elimination mechanismHis
  • D00244; Creatinase
    (Catalytic domain: 3.90.230.10)
  • 5.201.1651000.1453Eliminative double-bond formation Amine group from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Base-Acid/Stabilizer; Inorganic-cofactor-assisted base deprotonation of deprotonation site of leaving group; Acid protonation to eliminated group; Inorganic-cofactor-assisted stabilization of intermediate; Bond cleavage of eliminated group and double-bond formation by E1cb-like reaction mechanismAsp/Glu + two manganese ions bound to His/Asp cluster
  • S00419; Arginase
    (Catalytic domain: 3.40.800.10)
  • 5.201.1660000.464Eliminative double-bond formation Amine group from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Base-Acid; Inorganic-cofactor-assisted base deprotonation of deprotonation site of leaving group; Acid protonation to eliminated group; Inorganic-cofactor-assisted stabilization of intermediate; Bond cleavage of eliminated group and double-bond formation by E1cb-like reaction mechanismGlu + zinc ion bound to Cys/His cluster
  • D00406; Cytidine deaminase
    (Catalytic domain: 3.40.140.10)
  • S00808; Deoxycytidylate deaminase
    (Catalytic domain: 3.40.140.10)
  • S00810; Guanine deaminase
    (Catalytic domain: 3.40.140.10)
  • 5.201.2781500.453Eliminative double-bond formation Amine group from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Adenosine deaminase 2nd reaction step-like mechanismAn acidic residue + His + zinc ion bound to His/(acidic residue) cluster
  • D00873; Guanine deaminase
    (Catalytic domain: 3.20.20.140)
  • S00232; Adenosine deaminase
    (Catalytic domain: 3.20.20.140)
  • 5.201.2781500.563Eliminative double-bond formation Amine group from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]Adenosine deaminase 2nd reaction step-like mechanismAn acidic residue + His + Fe(II) ion bound to His/(acidic residue) cluster
  • D00675; Cytosine deaminase
    (Catalytic domain: 3.20.20.140)
  • 5.202.1504000.1Eliminative double-bond formation Amine group from Carbon (sp3) (with amine group) [leading to imine group]Acid; Interaction with enzyme and substrate/intermediate causes constraints in reactive parts & facilitates bond cleavage; protonation to eliminated group by acid through a water completes reaction; E1-like reaction mechanismAn acidic residue
  • S00276; Deoxycytidylate 5-hydroxymethyltransferase
    (Catalytic domain: 3.30.572.10)
  • 5.203.8000.93Eliminative double-bond formation Amine group from Carbon (sp3) (with carboxylate group)Base/Acid/Stabilizer/Modulator; Modulater-activated base deprotonates deprotonation site; Intermediate formation stabilized; Acid protonates eliminated group leading to cleavage; E1Cb-like reaction mechanismHis/Ser/Lys/Glu
  • T00094; Argininosuccinate lyase
    (Catalytic domain: 1.20.200.10)
  • 5.204.2187010.9090Eliminative double-bond formation Amine group from Carbon (sp3) adjacent to double-bondStabilizer/Electrophile; Initial state is electrophilic anion (no need to deprotonation of deprotonation site); Electrophilic modified residue-assisted bond cleavage; Eliminated group stabilized; E1cb-like reaction; mechanismTyr/Asn/Asn + MIO (Ala-Ser-Gly modified residues)
  • D00267; Histidine ammonia-lyase
    (Catalytic domains: 1.10.275.10, 1.20.200.10)
  • 5.300.550500.6111Eliminative double-bond formation Phosphate group from Carbon (sp3)Base/Acid/Modulator; Modulator-assisted base deprotonation of deprotonation site through water leading to bond cleavage; Acid protonation to eliminated group; E2-like reaction mechanismTwo acidic residues + Lys double-bonded to substrate/intermediate
  • D00266; Endonuclease III
    (Catalytic domains: 1.10.340.30, 1.10.1670.10)
  • 5.303.667700.54Eliminative double-bond formation Phosphate group from Carbon (sp3) (with carboxylate group)Base/Stabilizer; Stabilization of eliminated group; Base deprotonation of deprotonation site of leaving group leading to bond cleavage; E2-like reaction mechanismCys + Arg/Lys cluster
  • D00415; UDP-N-acetylglucosamine 1-carboxyvinyltransferase
    (Catalytic domain: 3.65.10.10)
  • 5.304.3380000.2135Eliminative double-bond formation Phosphate group from Carbon (sp3) adjacent to double-bondStabilizer; Inorganic cofactor-assisted stabilization of eliminated group & Leaving group stabilized; Bond-cleavage occurs by E1-like reaction mechanismLys/Arg cluster + Thr/Gln + mainchain carbonyl + three magnesium ions bound to acidic residues
  • S00024; Farnesyl pyrophosphate synthetase
    (Catalytic domain: 1.10.600.10)
  • 5.305.2200100.675Eliminative double-bond formation Phosphate group from Carboxylate (sp2) [release of CO2]Base-Acid/Stabilizer; Base deprotonation of deprotonation site of leaving group; Bond cleavage of eliminated group and double-bond formation by E2-like reaction; Inorganic-cofactor-assisted stabilization of eliminated intermediate: Acid protonation to eliminated intermediate mechanismHis/Arg + hydrophobic residues + divalent metal ion bound to acidic residues
  • T00217; Phosphoenolpyruvate carboxylase
    (Catalytic domains: 1.20.1100.10, 3.20.20.60)
  • 5.501.400060.67Eliminative double-bond formation Sulfide from Carbon (sp3) (with hydroxyl group) [leading to carbonyl group]HMG-CoA reductase class II 2nd reaction step-like mechanismHis/Lys + two acidic residues
  • D00607; 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    (Catalytic domain: 3.90.770.10)
  • M00180; 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    (Catalytic domains: 3.30.70.420, 3.90.770.10)
  • 5.524.3198500.5541Eliminative double-bond formation Sulfoxide group from Carbon (sp3) adjacent to double-bondAllinase 3rd reaction step-like mechanismLys + an acidic residue + Tyr/Tyr + hydroxyl residue (Ser or Thr) + mainchain amide + PLP covalently bound to substrate/intermediate
  • T00407; Alliin lyase 1
    (Catalytic domains: 2.10.25.30, 3.40.640.10)
  • 5.1020.2074300.8301Eliminative double-bond formation O-glycoside group from Carbon (sp3) adjacent to double-bond: Non-beta elimination6-phospho-alpha-glucosidase 3rd reaction step-like mechanismAn acidic residue + manganese ion bound to Cys/His/O7-nicotinamide/substrate or intermediate
  • D00827; Maltose-6''-phosphate glucosidase
    (Catalytic domain: 3.90.110.10)
  • 5.1202.1504200.6501Eliminative double-bond formation Amine group from Carbon (sp3) adjacent to double-bond with amine group [leading to imine group]: Non-beta elimination Acid; Electrophilic leaving group; Acid protonation to eliminated group completes reaction; E1-like reaction mechanismLys + Lys covalently bound to substrate/intermediate
  • S00239; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00538; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • 5.1204.671000.4100Eliminative double-bond formation Amine group from Carbon (sp3) adjacent to double-bond: Non-beta eliminationBase/Modulator; Modulator-assisted base deprotonation of deprotonation site of leaving group leading to bond cleavage; E2-like reaction mechanismTwo acidic residues + Cys covalently bound to substrate/intermediate
  • S00276; Deoxycytidylate 5-hydroxymethyltransferase
    (Catalytic domain: 3.30.572.10)
  • 5.1304.666000.68Eliminative double-bond formation Phosphate group from Carbon (sp3) adjacent to double-bond: Non-beta eliminationBase/Modulator/Stabilizers; Modulator-activated base deprotonates deprotonation site leading to bond cleavage; Eliminated group & leaving group stabilized; E2-like reaction mechanismAsp/Asp/His/His + Lys + Thr/Ser cluster + mainchain amide
  • S00370; Methylglyoxal synthase
    (Catalytic domain: 3.40.50.1380)
  • 5.1304.3086000.333Eliminative double-bond formation Phosphate group from Carbon (sp3) adjacent to double-bond: Non-beta eliminationStabilizer; Inorganic cofactor-assisted stabilization of eliminated group; Bond-cleavage occurs by E1-like reaction; Intermediate formation; Substrate-assisted base deprotonation of deprotonation site of leaving group mechanismArg/Lys cluster + Gln/Ser + magnesium ion bound to acidic residues
  • S00220; Thiamine-phosphate pyrophosphorylase
    (Catalytic domain: 3.20.20.70)
  • 5.1504.667700.4100Eliminative double-bond formation Sulfide from Carbon (sp3) adjacent to double-bond: Non-beta eliminationBase/Stabilizer; Stabilization of eliminated group; Base deprotonation of deprotonation site of leaving group leading to bond cleavage; E2-like reaction mechanismGlu/Arg + mainchain carbonyl + Cys covalently bound to substrate/intermediate
  • D00082; Modification methylase HaeIII
    (Catalytic domain: 3.40.50.150)
  • 6.10.82600.5900Exchange of double-bonded atoms Carbonyl oxygen to Schiff-base nitrogen (Schiff-base formation; dehydration)Acid-Base-Acid/Modulator; Substituted group attacks on double-bond partner atom; Acid protonation to leaving group; Formation of tetrahedral intermediate; Base deprotonation of substituted group; Lone pair on nucleophile attacks again on double-bond partner atom & 2nd Acid protonation to leaving group; Organic-cofactor-assisted reaction mechanismLys/Tyr + PMP bound
  • D00101; Aspartate aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00102; Tyrosine aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00108; Aromatic-amino-acid aminotransferase
    (Catalytic domain: 3.40.640.10)
  • 6.10.85210.5901Exchange of double-bonded atoms Carbonyl oxygen to Schiff-base nitrogen (Schiff-base formation; dehydration)Base/Acid/Modulator; Base-activated substituted group attacks on double-bond partner atom; Acid protonation to leaving group; Formation of tetrahedral intermediate; 2nd base deprotonation of substituted group; Lone pair on nucleophile attacks again on double-bond partner atom; Proton transfer from protonated base to deprotonated acid through cofactor/modulator and water; Organic-cofactor-assisted reaction mechanismLys/His/Tyr + PMP bound
  • D00105; D-alanine aminotransferase
    (Catalytic domains: 3.20.10.10, 3.30.470.10)
  • 6.10.398000.111Exchange of double-bonded atoms Carbonyl oxygen to Schiff-base nitrogen (Schiff-base formation; dehydration)Nucleophile/Acid-Base-Modulator-Stabilizer; Modulator-assisted nucleophile attacks on double-bond partner atom; Formation of tetrahedral transition-state; Transition-state stabilized; 1st general acid protonates tetrahedral transition-state; Base deprotonates 2nd Nucleophile; Lone-pair of 2nd Nucleophile attacks on center atom of tetrahedral intermediate; 2nd acid protonates leaving group leading to elimination of leaving group; SN2 & E2 mechanismLys/Lys
  • S00239; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00537; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00538; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00539; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • 6.10.400000.116Exchange of double-bonded atoms Carbonyl oxygen to Schiff-base nitrogen (Schiff-base formation; dehydration)Nucleophile/Base-Acid/Stabilizer-Modulator; Base activation of nucleophile through a modulated water; activated nucleophile attacks on double-bond partner atom; Stabilization of tetrahedral intermediate; Proton transfer from Nucleophile to leaving group through water; Acid protonation to leaving group mechanismLys/Glu/Thr
  • S00198; Transaldolase B
    (Catalytic domain: 3.20.20.70)
  • 6.10.400200.114Exchange of double-bonded atoms Carbonyl oxygen to Schiff-base nitrogen (Schiff-base formation; dehydration)Nucleophile-Acid/Base-Acid-Stabilizer; Base-activated nucleophile attacks on double-bond partner atom; Formation of tetrahedral intermediate; Nucleophile also acts as 1st general acid to protonate leaving group; 2nd Acid protonates leaving group mechanismLys/Glu
  • S00222; KHG/KDPG aldolase
    (Catalytic domain: 3.20.20.70)
  • 6.10.400600.113Exchange of double-bonded atoms Carbonyl oxygen to Schiff-base nitrogen (Schiff-base formation; dehydration)Nucleophile/Base-Acid/Modulator; Modulator-assisted base activation of nucleophile; nucleophile attacks on double-bonded partner atom; Acid-Base transfers proton from the nucleophile to leaving group; Acid protonation to leaving group completing reaction mechanismLys + two acidic residues
  • S00199; Fructose-bisphosphate aldolase A
    (Catalytic domain: 3.20.20.70)
  • 6.10.431300.120Exchange of double-bonded atoms Carbonyl oxygen to Schiff-base nitrogen (Schiff-base formation; dehydration)Nucleophile/Substrate-assisted-Acid-Base-Stabilizer; nucleophile attacks on double-bond partner atom; Formation of tetrahedral transition-state; Transition-state stabilized; 1st substrate-acid protonates tetrahedral transition-state; Substrate-base deprotonates 2nd Nucleophile; Lone-pair of 2nd Nucleophile attacks on center atom of tetrahedral intermediate; 2nd substrate-acid protonates leaving group leading to elimination of leaving group; SN2 & E2 mechanismLys
  • S00239; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00537; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00538; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00539; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • 6.10.445000.115Exchange of double-bonded atoms Carbonyl oxygen to Schiff-base nitrogen (Schiff-base formation; dehydration)Nucleophile-Acid/Stabilizer; Nucleophile attacks on double-bond partner atom; Formation of tetrahedral intermediate; Nucleophile also acts as 1st general acid to protonate leaving group; Same acid protonates leaving group leading to elimination of leaving group; Indirect stabilization mechanismLys + mainchain carbonyl
  • S00200; N-acetylneuraminate lyase
    (Catalytic domain: 3.20.20.70)
  • 6.10.448000.112Exchange of double-bonded atoms Carbonyl oxygen to Schiff-base nitrogen (Schiff-base formation; dehydration)Nucleophile-Acid/Acid/Modulator; Nucleophile attacks on double-bond partner atom; Formation of tetrahedral intermediate; Nucleophile also acts as 1st general acid to protonate leaving group; A second acid protonates leaving group leading to elimination of leaving group mechanismLys/His/Glu
  • S00240; 3-dehydroquinate dehydratase
    (Catalytic domain: 3.20.20.70)
  • 6.20.7100.6120Exchange of double-bonded atoms Schiff-base nitrogen to Carbonyl oxygen (Schiff-base deformation; hydration)Base-Acid/Stabilizer-Modulator; Base activation of substituted group through a water; substituted group attacks on double-bonded partner atom; Stabilization of tetrahedral intermediate; Proton transfer from the substituted group to leaving group through water; Acid protonation to leaving group mechanismGlu/Thr + Lys double-bonded to substrate/intermediate
  • S00198; Transaldolase B
    (Catalytic domain: 3.20.20.70)
  • 6.20.7800.6111Exchange of double-bonded atoms Schiff-base nitrogen to Carbonyl oxygen (Schiff-base deformation; hydration)Base-Acid/Modulator; Modulator-assisted base activation of substituted group; substituted group attacks on double-bonded partner atom; Acid-Base transfers proton from the substituted group to leaving group; Acid protonation to leaving group mechanismTwo acidic residues + Lys double-bonded to substrate/intermediate
  • S00199; Fructose-bisphosphate aldolase A
    (Catalytic domain: 3.20.20.70)
  • 6.20.8000.6100Exchange of double-bonded atoms Schiff-base nitrogen to Carbonyl oxygen (Schiff-base deformation; hydration)Base-Acid/Base; Base activation of substituted group; Substituted group attacks on double-bond partner atom; Formation of tetrahedral intermediate; Covalently-bonded base deprotonates substituted group; Lone pair on substituted group attacks again on double-bond partner atom leading to bond cleavage; Acid protonation to leaving group mechanismAn acidic residue + Lys double-bonded to substrate/intermediate
  • S00222; KHG/KDPG aldolase
    (Catalytic domain: 3.20.20.70)
  • 6.20.8010.6100Exchange of double-bonded atoms Schiff-base nitrogen to Carbonyl oxygen (Schiff-base deformation; hydration)Base-Acid; Base activation of substituted group; substituted group attacks on double-bonded partner atom; Acid-Base transfers proton from the substituted group to leaving group; Acid protonation to leaving group mechanismAn acidic residue + Lys double-bonded to substrate/intermediate
  • D00266; Endonuclease III
    (Catalytic domains: 1.10.340.30, 1.10.1670.10)
  • 6.20.28500.6010Exchange of double-bonded atoms Schiff-base nitrogen to Carbonyl oxygen (Schiff-base deformation; hydration)Base/Modulator/Base; Base activation of substituted group; Substituted group attacks on double-bond partner atom; Formation of tetrahedral intermediate; Covalently-bonded base deprotonates substituted group; Lone pair on nucleophile (substituted group) attacks again on double-bond partner atom mechanismHis/Glu + Lys double-bonded to substrate/intermediate
  • S00240; 3-dehydroquinate dehydratase
    (Catalytic domain: 3.20.20.70)
  • 6.20.29000.6000Exchange of double-bonded atoms Schiff-base nitrogen to Carbonyl oxygen (Schiff-base deformation; hydration)Base; Substituted group attacks on double-bond partner atom; Formation of tetrahedral intermediate; Covalently-bonded base deprotonates substituted group; Lone pair on nucleophile attacks again on double-bond partner atom mechanismLys double-bonded to substrate/intermediate
  • S00200; N-acetylneuraminate lyase
    (Catalytic domain: 3.20.20.70)
  • 6.20.85200.5520Exchange of double-bonded atoms Schiff-base nitrogen to Carbonyl oxygen (Schiff-base deformation; hydration)Base-Acid-Base/Modulator; Base-activated substituted group attacks on double-bond partner atom; Acid protonation to leaving group; Formation of tetrahedral intermediate; 2nd base deprotonation of substituted group; Lone pair on nucleophile attacks again on double-bond partner atom; Organic-cofactor-assisted reaction mechanismLys/Tyr + PLP double-bonded to substrate/intermediate
  • D00101; Aspartate aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00102; Tyrosine aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00108; Aromatic-amino-acid aminotransferase
    (Catalytic domain: 3.40.640.10)
  • 6.20.85210.5511Exchange of double-bonded atoms Schiff-base nitrogen to Carbonyl oxygen (Schiff-base deformation; hydration)Base/Acid/Modulator; Base-activated substituted group attacks on double-bond partner atom; Acid protonation to leaving group; Formation of tetrahedral intermediate; 2nd base deprotonation of substituted group; Lone pair on nucleophile attacks again on double-bond partner atom; Proton transfer from protonated base to deprotonated acid through cofactor/modulator and water; Organic-cofactor-assisted reaction mechanismHis/Lys/Tyr + PLP double-bonded to substrate/intermediate
  • D00105; D-alanine aminotransferase
    (Catalytic domains: 3.20.10.10, 3.30.470.10)
  • 6.20.93000.5520Exchange of double-bonded atoms Schiff-base nitrogen to Carbonyl oxygen (Schiff-base deformation; hydration)Base/Modulator; Base-activated substituted group attacks on double-bond partner atom; Formation of tetrahedral intermediate; Organic-cofactor-assisted deprotonation of 3rd base; 3rd base activation of lone pair on nucleophile; Lone pair on nucleophile attacks again on doulbe-bond partner atom; Organic-cofactor-assisted protonation to leaving group mechanismLys/Tyr + PLP double-bonded to substrate/intermediate
  • D00106; Branched-chain-amino-acid aminotransferase
    (Catalytic domain: 3.20.10.10)
  • 6.30.83700.5070Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (external aldimine formation)Base-Acid-Base; Base-activated substituted group attacks on double-bond partner atom; Acid protonation to leaving group; Formation of tetrahedral intermediate; 2nd base deprotonation of substituted group; Lone pair on nucleophile attacks again on double-bond partner atom; Organic-cofactor-assisted reaction mechanismAn acidic residue + PLP double-bonded to Lys
  • T00089; Tryptophan synthase alpha chain
    (Catalytic domains: 3.20.20.70, 3.40.50.1100)
  • 6.30.93020.5311Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (external aldimine formation)Base/Modulator; Modulator-assisted base activation of substituted group attacks on double-bond partner atom; Formation of tetrahedral intermediate; Organic-cofactor-assisted deprotonation of nucleophile & protonation to leaving group; Lone pair on nucleophile attacks again on doulbe-bond partner atom mechanismTyr +Arg/His/Asp + PLP double-bonded to Lys
  • D00271; Alanine racemase
    (Catalytic domains: 2.40.37.10, 3.20.20.10)
  • 6.30.97700.5320Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (external aldimine formation)Base/Modulator; Organic-cofactor/Modulator-assisted proton transfer from substituted group to leaving group; Nucleophilic attack of substituted group on double-bond partner atom; Formation of tetrahedral intermediate; Lone pair on nucleophile attacks again on double-bond partner atom mechanismTyr/Tyr + PLP double-bonded to Lys
  • D00101; Aspartate aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00102; Tyrosine aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00108; Aromatic-amino-acid aminotransferase
    (Catalytic domain: 3.40.640.10)
  • T00407; Alliin lyase 1
    (Catalytic domains: 2.10.25.30, 3.40.640.10)
  • 6.30.97710.5300Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (external aldimine formation)Base/Modulator; Organic-cofactor/Modulator-assisted proton transfer from substituted group to leaving group through water; Nucleophilic attack of substituted group on double-bond partner atom; Formation of tetrahedral intermediate; Lone pair on nucleophile attacks again on double-bond partner atom mechanismTyr + PLP double-bonded to Lys
  • D00105; D-alanine aminotransferase
    (Catalytic domains: 3.20.10.10, 3.30.470.10)
  • 6.30.100000.5310Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (external aldimine formation)Base/Modulator; Base-activated substituted group attacks on double-bond partner atom; Formation of tetrahedral intermediate; Lone pair on nucleophile attacks again on double-bond partner atom; Organic-cofactor-assisted reaction mechanismTyr/Arg + PLP double-bonded to Lys
  • D00265; Cystathionine gamma-synthase
    (Catalytic domain: 3.40.640.10)
  • 6.30.115000.5080Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (external aldimine formation)Base/Modulator; Cofactor-assisted activation of substituted group; Activated substituted group attacks on double-bond partner atom; Formation of tetrahedral intermediate; Base-activation of nucleophile; Lone pair on nucleophile attacks again on double-bond partner atom mechanismHis + His/Ser + an acidic residue + PLP double-bonded to Lys
  • D00092; 8-amino-7-oxononanoate synthase
    (Catalytic domains: 3.40.640.10, 3.90.1150.10)
  • D00639; 2-amino-3-ketobutyrate coenzyme A ligase
    (Catalytic domain: 3.40.640.10)
  • 6.30.115020.5300Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (external aldimine formation)Modulator; Oraganic-cofactor/substrate-assisted activation of substituted group; Activated substituted group attacks on double-bond partner atom; Formation of tetrahedral intermediate; Cofactor-assisted activation of nucleophile; Lone pair on nucleophile attacks again on doulbe-bond partner atom mechanismTyr + PLP double-bonded to Lys
  • D00106; Branched-chain-amino-acid aminotransferase
    (Catalytic domain: 3.20.10.10)
  • 6.30.264850.5200Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (external aldimine formation)Modulator; Oraganic-cofactor/substrate-assisted activation of substituted group; Activated substituted group attacks on double-bond partner atom; Formation of tetrahedral intermediate; Modulator/Cofactor-assisted activation of nucleophile; Lone pair on nucleophile attacks again on double-bond partner atom mechanismHydroxyl residue (Thr or Ser) + PLP double-bonded to Lys
  • D00085; Serine hydroxymethyltransferase
    (Catalytic domain: 3.40.640.10)
  • 6.40.477300.5510Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (internal aldimine formation)Nucleophile/Base-Acid-Base; Base-activated nucleophile attacks on double-bond partner atom; Acid protonation to leaving group; Formation of tetrahedral intermediate; 2nd base deprotonation of nucleophile; Lone pair on nucleophile attacks again on double-bond partner atom; Organic-cofactor-assisted reaction mechanismLys/His + PLP double-bonded to substrate/intermediate
  • T00089; Tryptophan synthase alpha chain
    (Catalytic domains: 3.20.20.70, 3.40.50.1100)
  • 6.40.500000.5600Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (internal aldimine formation)Nucleophile/Acid/Modulator; Nucleophile attacks on double-bond partner atom; Formation of tetrahedral intermediate; protonation to leaving group; Organic-cofactor-assisted activation of nucleophile; Lone pair on nucleophile attacks on double-bond partner atom mechanismLys/His + His/Ser + an acidic residue + PLP double-bonded to substrate/intermediate
  • D00092; 8-amino-7-oxononanoate synthase
    (Catalytic domains: 3.40.640.10, 3.90.1150.10)
  • D00639; 2-amino-3-ketobutyrate coenzyme A ligase
    (Catalytic domain: 3.40.640.10)
  • 6.40.508000.5522Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (internal aldimine formation)Nucleophile/Acid/Modulator; Nucleophile attacks on double-bond partner atom; Formation of tetrahedral intermediate; Organic-cofactor-assisted protonation to leaving group & deprotonation of nucleophile; Lone pair on nucleophile attacks on double-bond partner atom mechanismLys/Tyr +Arg/His/Asp + PLP double-bonded to substrate/intermediate
  • D00271; Alanine racemase
    (Catalytic domains: 2.40.37.10, 3.20.20.10)
  • 6.40.521000.5530Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (internal aldimine formation)Nucleophile/Acid/Modulator; Nucleophile attacks on double-bond partner atom; Acid protonation to leaving group; Formation of tetrahedral intermediate; Lone pair on nucleophile attacks on double-bond partner atom; Organic-cofactor/Modulator-assisted proton transfer from nucleophile to leaving group mechanismLys/Tyr/Tyr + PLP double-bonded to substrate/intermediate
  • D00101; Aspartate aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00102; Tyrosine aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00108; Aromatic-amino-acid aminotransferase
    (Catalytic domain: 3.40.640.10)
  • T00407; Alliin lyase 1
    (Catalytic domains: 2.10.25.30, 3.40.640.10)
  • 6.40.521010.5520Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (internal aldimine formation)Nucleophile-Acid/Modulator; Nucleophile attacks on double-bond partner atom; Acid protonation to leaving group through water; Formation of tetrahedral intermediate; Lone pair on nucleophile attacks on double-bond partner atom; Organic-cofactor/Modulator-assisted proton transfer from nucleophile to leaving group through water mechanismLys/Tyr + PLP double-bonded to substrate/intermediate
  • D00105; D-alanine aminotransferase
    (Catalytic domains: 3.20.10.10, 3.30.470.10)
  • 6.40.528600.5540Exchange of double-bonded atoms Exchange of Schiff-base nitrogen atoms (internal aldimine formation)Nucleophile/Modulator; Modulator-assisted nucleophile attacks on double-bond partner atom; Formation of tetrahedral intermediate; Organic-cofactor-assisted proton transfer between nucleophile and leaving group; Lone pair on nucleophile attacks on double-bond partner atom; substrate-assisted protonation to leaving group mechanismLys + hydroxyl residue (Thr or Ser) + PLP double-bonded to substrate/intermediate
  • D00085; Serine hydroxymethyltransferase
    (Catalytic domain: 3.40.640.10)
  • 8.112.3600000.6580Isomerization Adjacent bond; C=C-N to C-C=NAcid/Stabilizer/Nucleophile; lone pair at nucleophile attacks on central atom; Intermediate stabilized; Acid protonation to initially-double-bonded atom mechanismAsp/Lys + Lys covalently bound to substrate/intermediate
  • S00199; Fructose-bisphosphate aldolase A
    (Catalytic domain: 3.20.20.70)
  • 8.113.42001.5Isomerization Adjacent bond; C=C-O to C-C=OBase-Acid/Stabilizers; Base deprotonation of initially single-bonded atom leading to intermediate formation; Intermediate stabilized; Acid protonation to initially double-bonded atom mechanismAn acidic residue + His/Asn
  • S00225; Triosephosphate isomerase
    (Catalytic domain: 3.20.20.70)
  • T00239; Bifunctional PGK/TIM
    (Catalytic domains: 3.20.20.70, 3.40.50.1260, 3.40.50.1270)
  • 8.113.46500.4Isomerization Adjacent bond; C=C-O to C-C=OMetal-independent eukaryotic CMLE-like mechanismAn acidic residue + His
  • S00852; Carboxy-cis,cis-muconate cyclase
    (Catalytic domain: 2.130.10.10)
  • 8.113.46500.7Isomerization Adjacent bond; C=C-O to C-C=OMetal-independent eukaryotic CMLE-like mechanismAn acidic residue + Lys
  • T00201; Glucosamine--fructose-6-phosphate aminotransferase {isomerizing}
    (Catalytic domains: 3.40.50.10490, 3.60.20.10)
  • 8.113.365000.100Isomerization Adjacent bond; C=C-O to C-C=OAcid/Modulator; Modulator-assisted acid protonation to initially-double-bonded atom; water molecules required for proton shuttle to recover catalytic residues mechanismTyr/Asn
  • S00155; dTDP-4-dehydrorhamnose 3,5-epimerase
    (Catalytic domain: 2.60.120.10)
  • 8.113.365001.100Isomerization Adjacent bond; C=C-O to C-C=OAcid/Modulator; Modulator-assisted acid protonation to initially-double-bonded atom through a water; water molecules required for proton shuttle to recover catalytic residues mechanismTyr/Asn
  • S00155; dTDP-4-dehydrorhamnose 3,5-epimerase
    (Catalytic domain: 2.60.120.10)
  • 8.113.580000.386Isomerization Adjacent bond; C=C-O to C-C=OBase-Stabilizer/Acid/Modulator/Stabilizer; Inorganic cofactor-assisted stabilization of adjacent atoms of central atom/initially single-bonded atom/double-bonded atom; Modulator-assisted acid protonation to initially double-bonded atom & Base deprotonation of initially single-bonded atom mechanismHis/Glu/Asp + Lys + magnesium ion bound to acidic residues
  • D00273; Mandelate racemase
    (Catalytic domain: 3.20.20.120)
  • 8.113.585900.479Isomerization Adjacent bond; C=C-O to C-C=OAcid-Base/Acid; Inorganic-cofactor-assisted stabilization of initially single-bonded atom and adjacent atom to double-bonded atom; Acid protonation to double-bonded atom & Base deprotonation of single-bonded atom; 2nd Acid protonation to adjacent atom to initially-double-bonded atom mechanismTwo acidic residues + zinc ion bound to Gln/His/Glu & substrate/intermediate
  • S00185; Lactoylglutathione lyase
    (Catalytic domain: 3.10.180.10)
  • 8.113.585900.720Isomerization Adjacent bond; C=C-O to C-C=OAcid-Base/Acid; Inorganic-cofactor-assisted stabilization of initially single-bonded atom and adjacent atom to double-bonded atom; Acid protonation to double-bonded atom & Base deprotonation of single-bonded atom; 2nd Acid protonation to adjacent atom to initially-double-bonded atom mechanismTwo acidic residues + nickel ion bound to His/Glu cluster & substrate/intermediate
  • S00540; Glyoxylase family protein
    (Catalytic domain: 3.10.180.10)
  • 8.113.594730.651Isomerization Adjacent bond; C=C-O to C-C=OMetal-dependent bacterial MLE-like mechanismAn acidic residue + Lys/Lys + manganese ion bound to acidic residues
  • D00282; Muconate cycloisomerase 1
    (Catalytic domain: 3.20.20.120)
  • D00283; Chloromuconate cycloisomerase
    (Catalytic domain: 3.20.20.120)
  • 8.113.901890.372Isomerization Adjacent bond; C=C-O to C-C=OGlucarate dehydratase 3rd reaction step-like mechanismHis + Tyr/Lys/Asn + magnesium ion bound to Asp/Glu/Asn
  • D00261; Glucarate dehydratase
    (Catalytic domain: 3.20.20.120)
  • 8.113.904850.8015Isomerization Adjacent bond; C=C-O to C-C=O6-phospho-alpha-glucosidase 5th reaction step-like mechanismTyr + Glu + manganese ion bound to Cys/His/O7-nicotinamide/substrate or intermediate
  • D00827; Maltose-6''-phosphate glucosidase
    (Catalytic domain: 3.90.110.10)
  • 8.113.904870.263Isomerization Adjacent bond; C=C-O to C-C=OMannose-6-phosphate isomerase 2nd reaction step-like mechanismGlu/Tyr + zinc ion bound to His/Glu/Gln cluster
  • T00101; Mannose-6-phosphate isomerase
    (Catalytic domain: 2.60.120.10)
  • 8.113.915300.451Isomerization Adjacent bond; C=C-O to C-C=OAcid/Stabilizer; Inorganic-cofactor-assisted stabilization of initially single-bonded atom; Acid protonation to double-bonded atom mechanismAn acidic residue + Asn (or Gln) + zinc ion bound to His cluster
  • S00235; Fructose-bisphosphate aldolase class 2
    (Catalytic domain: 3.20.20.70)
  • 8.113.915310.365Isomerization Adjacent bond; C=C-O to C-C=OAcid/Proton_shuttle/Stabilizer; Inorganic-cofactor-assisted stabilization of initially single-bonded atom; Acid protonation to double-bonded atom through proton-shuttle mechanismGlu + Thr/Ser + Lys + magnesium ion bound to acidic residues
  • T00043; Pyruvate kinase I
    (Catalytic domains: 2.40.33.10, 3.20.20.60)
  • 8.113.919760.1180Isomerization Adjacent bond; C=C-O to C-C=OAcid; Inorganic-cofactor-assisted stabilization of initially single-bonded atom; Acid protonation to double-bonded atom mechanismLys + two magnesium ions bound to acidic residues
  • S00841; Phosphosulfolactate synthase
    (Catalytic domain: 3.20.20.70)
  • 8.113.1441400.8202Isomerization Adjacent bond; C=C-O to C-C=OGDP fucose synthase 2nd reaction step-like mechanismHis + Tyr/Lys bound to ribose-nicotinamide
  • D00604; GDP-L-fucose synthetase
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • 8.121.42000.7Isomerization Adjacent bond; N=C-C to N-C=CBase/Acid-Stabilizer; Base deprotonation of initially single-bonded atom leading to intermediate formation; Intermediate stabilized; Acid protonation to initially double-bonded atom mechanismAn acidic residue + Lys
  • T00201; Glucosamine--fructose-6-phosphate aminotransferase {isomerizing}
    (Catalytic domains: 3.40.50.10490, 3.60.20.10)
  • 8.121.166300.1Isomerization Adjacent bond; N=C-C to N-C=CBase; Base deprotonation of initially-single-bonded atom completes reaction mechanismAn acidic residue
  • S00226; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
    (Catalytic domain: 3.20.20.70)
  • 8.121.166300.8Isomerization Adjacent bond; N=C-C to N-C=CBase; Base deprotonation of initially-single-bonded atom completes reaction mechanismCys
  • S00847; N-(5''-phosphoribosyl)anthranilate isomerase
    (Catalytic domain: 3.20.20.70)
  • 8.121.1440000.6450Isomerization Adjacent bond; N=C-C to N-C=CInorganic-cofactor-assisted base; Base bound to inroganic-cofactor deprotonation of originally single-bonded atom leading to isomerization mechanismHydroxide bound to Zinc bound to Cys cluster + Lys double-bonded to substrate/intermediate
  • S00239; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00538; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • 8.131.42001.6Isomerization Adjacent bond; O=C-C to O-C=CBase-Acid/Stabilizers; Base deprotonation of initially single-bonded atom leading to intermediate formation; Intermediate stabilized; Acid protonation to initially double-bonded atom mechanismAn acidic residue + His/Lys
  • S00225; Triosephosphate isomerase
    (Catalytic domain: 3.20.20.70)
  • T00239; Bifunctional PGK/TIM
    (Catalytic domains: 3.20.20.70, 3.40.50.1260, 3.40.50.1270)
  • 8.131.160500.80Isomerization Adjacent bond; O=C-C to O-C=CBase/Modulator/Stabilizer; Modulator-assisted base deprotonation of initially-single-bonded atom; Initially-double-bonded atom stabilized mechanismHis/Asp/Lys
  • S00155; dTDP-4-dehydrorhamnose 3,5-epimerase
    (Catalytic domain: 2.60.120.10)
  • 8.131.161950.140Isomerization Adjacent bond; O=C-C to O-C=CDodecenoyl-CoA delta-isomerase 1st reaction step-like mechanismAn acidic residue + mainchain amide groups
  • S00849; 3,2-trans-enoyl-CoA isomerase (EC 5.3.3.8) (Dodecenoyl-CoA isomerase) (Delta
    (Catalytic domain: 3.90.226.10)
  • 8.131.164850.131Isomerization Adjacent bond; O=C-C to O-C=CGDP mannose dehydratase 2nd reaction step-like mechanismAn acidic residue + Arg
  • D00513; GDP-mannose 4,6 dehydratase 2
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • D00543; GDP-mannose 4,6-dehydratase
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • 8.131.581400.399Isomerization Adjacent bond; O=C-C to O-C=CBase/Acid-Stabilizer/Stabilizer; Inorganic cofactor-assisted stabilization of adjacent atoms of central atom/initially single-bonded atom/double-bonded atom; Base deprotonation of initially single-bonded atom & Acid protonation to initially double-bonded atom mechanismLys/Glu + Lys + magnesium ion bound to acidic residues
  • D00273; Mandelate racemase
    (Catalytic domain: 3.20.20.120)
  • 8.131.584400.264Isomerization Adjacent bond; O=C-C to O-C=CBase/Base-Acid/Modulator; Modulator-assisted base deprotonation of initially single-bonded atom; Inorganic cofactor-assisted proton transfer by base-acid from atom adjacent to initially single-bonded atom to initially double-bonded atom mechanismGlu/Lys/Tyr + zinc ion bound to His/Glu/Gln cluster
  • T00101; Mannose-6-phosphate isomerase
    (Catalytic domain: 2.60.120.10)
  • 8.131.704320.8015Isomerization Adjacent bond; O=C-C to O-C=C6-phospho-alpha-glucosidase 2nd reaction step-like mechanismTyr + Glu + manganese ion bound to Cys/His/O7-nicotinamide/substrate or intermediate
  • D00827; Maltose-6''-phosphate glucosidase
    (Catalytic domain: 3.90.110.10)
  • 8.131.705800.452Isomerization Adjacent bond; O=C-C to O-C=CBase; Inorganic-cofactor-assisted stabilization of double-bonded atom and adjacent atom to single-bonded atom; Inorganic-cofactor-assisted activation of initially single-bonded atom; Base deprotonation of single-bonded atom mechanismAn acidic residue + zinc ion bound to Gln/His/Glu & substrate/intermediate
  • S00185; Lactoylglutathione lyase
    (Catalytic domain: 3.10.180.10)
  • 8.131.705800.710Isomerization Adjacent bond; O=C-C to O-C=CBase; Inorganic-cofactor-assisted stabilization of double-bonded atom and adjacent atom to single-bonded atom; Inorganic-cofactor-assisted activation of initially single-bonded atom; Base deprotonation of single-bonded atom mechanismAn acidic residue + nickel ion bound to His/Glu cluster & substrate/intermediate
  • S00540; Glyoxylase family protein
    (Catalytic domain: 3.10.180.10)
  • 8.131.708000.451Isomerization Adjacent bond; O=C-C to O-C=CBase/Stabilizer; Inorganic-cofactor-bound to double-bonded atom; Base deprotonation of initially single-bonded atom leading to isomerization; Stabilization of charge on finally single-bonded atom mechanismAn acidic residue + Asn (or Gln) + zinc ion bound to His cluster
  • S00235; Fructose-bisphosphate aldolase class 2
    (Catalytic domain: 3.20.20.70)
  • 8.131.712490.370Isomerization Adjacent bond; O=C-C to O-C=CGlucarate dehydratase 1st reaction step-like mechanismHis/Lys + magnesium ion bound to Asp/Glu/Asn
  • D00261; Glucarate dehydratase
    (Catalytic domain: 3.20.20.120)
  • 8.131.1241100.8201Isomerization Adjacent bond; O=C-C to O-C=CGDP fucose synthase 1st reaction step-like mechanismCys + Tyr/Lys bound to ribose-nicotinamide
  • D00604; GDP-L-fucose synthetase
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • 8.211.591500.5520Isomerization Adjacent bond; C=N-C to C-N=CBase-Acid/Modulator; Organic-cofactor-bound to central atom; Modulator-assisted base deprotonation of initially single-bonded atom leading to intermediate; Modulator-assisted acid protonation to initially double-bonded atom mechanismLys/Tyr + PLP double-bonded to substrate/intermediate
  • D00265; Cystathionine gamma-synthase
    (Catalytic domain: 3.40.640.10)
  • 8.211.591500.5521Isomerization Adjacent bond; C=N-C to C-N=CBase-Acid/Modulator; Organic-cofactor-bound to central atom; Modulator-assisted base deprotonation of initially single-bonded atom leading to intermediate; Modulator-assisted acid protonation to initially double-bonded atom mechanismLys/Tyr + PLP covalently bound to substrate/intermediate
  • D00265; Cystathionine gamma-synthase
    (Catalytic domain: 3.40.640.10)
  • 8.211.591510.5526Isomerization Adjacent bond; C=N-C to C-N=CBase-Acid/Modulator; Organic-cofactor-bound to central atom; Modulator-assisted stabilization of organic cofactor; Base deprotonation of initially single-bonded atom leading to intermediate; Acid protonation to initially double-bonded atom mechanismLys + an acidic residue + Tyr + PLP double-bonded to substrate/intermediate
  • D00101; Aspartate aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00102; Tyrosine aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00105; D-alanine aminotransferase
    (Catalytic domains: 3.20.10.10, 3.30.470.10)
  • D00108; Aromatic-amino-acid aminotransferase
    (Catalytic domain: 3.40.640.10)
  • 8.211.591510.5527Isomerization Adjacent bond; C=N-C to C-N=CBase-Acid/Modulator; Organic-cofactor-bound to central atom; Modulator-assisted stabilization of organic cofactor; Base deprotonation of initially single-bonded atom leading to intermediate; Acid protonation to initially double-bonded atom mechanismLys + an acidic residue + Tyr + PLP covalently bound to substrate/intermediate
  • D00101; Aspartate aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00102; Tyrosine aminotransferase
    (Catalytic domain: 3.40.640.10)
  • D00105; D-alanine aminotransferase
    (Catalytic domains: 3.20.10.10, 3.30.470.10)
  • D00108; Aromatic-amino-acid aminotransferase
    (Catalytic domain: 3.40.640.10)
  • 8.11113.45000.86Isomerization Non-adjacent bond; C=C-C=C-O to C-C=C-C=OBase-Acid/Modulator; Modulator-assisted base-acid; Base deprotonation of initially single-bonded atom leading to intermediate formation; Acid protonation to initially double-bonded atom mechanismHis/Asp
  • S00355; 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
    (Catalytic domain: 3.40.50.1820)
  • S00526; 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
    (Catalytic domain: 3.40.50.1820)
  • 8.11113.362040.140Isomerization Non-adjacent bond; C=C-C=C-O to C-C=C-C=ODodecenoyl-CoA delta-isomerase 2nd reaction step-like mechanismAn acidic residue + mainchain amide groups
  • S00849; 3,2-trans-enoyl-CoA isomerase (EC 5.3.3.8) (Dodecenoyl-CoA isomerase) (Delta
    (Catalytic domain: 3.90.226.10)
  • 8.11121.166400.120Isomerization Non-adjacent bond; N=C-C=C-C to N-C=C-C=CBase; Electrophilic dobule-bonded atom; Electrophile-assisted reaction; Base deprotonation of initially-single-bonded atom completes reaction mechanismLys
  • S00239; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00537; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00538; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • S00539; Delta-aminolevulinic acid dehydratase
    (Catalytic domain: 3.20.20.70)
  • 8.11131.365050.86Isomerization Non-adjacent bond; O=C-C=C-C to O-C=C-C=CAcid/Modulator; Bond cleavage adjacent to single-bonded atom triggers reaction; Modulator-assisted acid protonation to initially-double-bonded atom mechanismHis/Asp
  • S00355; 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
    (Catalytic domain: 3.40.50.1820)
  • S00526; 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
    (Catalytic domain: 3.40.50.1820)
  • 8.11211.912000.5510Isomerization Non-adjacent bond; C=N-C=C-C to C-N=C-C=CAcid/Modulator; Organic-cofactor-bound to central atom; Modulator-assisted acid protonation to initially double-bonded atom mechanismLys/His + PLP double-bonded to substrate/intermediate
  • T00089; Tryptophan synthase alpha chain
    (Catalytic domains: 3.20.20.70, 3.40.50.1100)
  • 8.11211.912000.5525Isomerization Non-adjacent bond; C=N-C=C-C to C-N=C-C=CAcid/Modulator; Organic-cofactor-bound to central atom; Modulator-assisted acid protonation to initially double-bonded atom mechanismLys+ Arg/Asp + PLP covalently bound to substrate/intermediate
  • D00271; Alanine racemase
    (Catalytic domains: 2.40.37.10, 3.20.20.10)
  • 8.11211.913550.5730Isomerization Non-adjacent bond; C=N-C=C-C to C-N=C-C=CAcid; Organic-cofactor-bound to central atom; Acid protonation to initially double-bonded atom mechanismTyr + PLP covalently bound to substrate/intermediate
  • D00085; Serine hydroxymethyltransferase
    (Catalytic domain: 3.40.640.10)
  • 8.12111.711000.5510Isomerization Non-adjacent bond; C=C-C=N-C to C-C=C-N=CBase/Modulator; Organic-cofactor-bound to central atom; Modulator-assisted base deprotonation of initially single-bonded atom mechanismLys/His + PLP double-bonded to substrate/intermediate
  • T00089; Tryptophan synthase alpha chain
    (Catalytic domains: 3.20.20.70, 3.40.50.1100)
  • 8.12111.792000.5722Isomerization Non-adjacent bond; C=C-C=N-C to C-C=C-N=CBase/Modulator; Organic-cofactor-bound to central atom; Substrate-assisted deprotonation & protonation (proton transfer) to activate 2nd base; Modulator-assisted base deprotonation of initially single-bonded atom mechanismTyr +Arg/His + PLP double-bonded to substrate/intermediate
  • D00271; Alanine racemase
    (Catalytic domains: 2.40.37.10, 3.20.20.10)
  • 8.1121121.660330.5528Isomerization Non-adjacent bond; N=C-C=C-C=N-C to N-C=C-C=C-N=CAllinase 2nd reaction step-like mechanismTyr/Lys + an acidic residue + Tyr + PLP double-bonded to substrate/intermediate
  • T00407; Alliin lyase 1
    (Catalytic domains: 2.10.25.30, 3.40.640.10)
  • 9.1050.192630.8500Hydride transferFrom CH-OH to nicotinamide (NAD+/NADP+)6-phospho-alpha-glucosidase 1st reaction step-like mechanismManganese ion bound to Cys/His/O7-nicotinamide/substrate or intermediate
  • D00827; Maltose-6''-phosphate glucosidase
    (Catalytic domain: 3.90.110.10)
  • 9.1050.439970.118Hydride transferFrom CH-OH to nicotinamide (NAD+/NADP+)Shikimate dehydrogenase-like oxidation mechanismLys/Asp
  • D00605; Quinate/shikimate dehydrogenase
    (Catalytic domain: 3.40.192.10)
  • D00845; Shikimate dehydrogenase
    (Catalytic domain: 3.40.192.10)
  • D00857; Shikimate 5-dehydrogenase-like protein HI0607
    (Catalytic domains: 3.40.50.720, 3.40.192.10)
  • D00858; Quinate/shikimate dehydrogenase
    (Catalytic domain: 3.40.192.10)
  • T00414; Fusion: chorismate mutase and shikimate 5-dehydrogenase
    (Catalytic domain: 3.40.192.10)
  • 9.1050.439980.119Hydride transferFrom CH-OH to nicotinamide (NAD+/NADP+)GDP mannose dehydrogenase 1st & 3rd reaction step-like mechanismLys + Asp/Thr bound to water
  • T00227; UDP-glucose 6-dehydrogenase
    (Catalytic domains: 1.10.1040.10, 3.40.50.720)
  • T00408; GDP-mannose 6-dehydrogenase
    (Catalytic domains: 1.-.-.-, 3.40.50.720)
  • 9.1050.440000.8010Hydride transferFrom CH-OH to nicotinamide (NAD+/NADP+)Drosophia alcohol dehydrogenase-like oxidation mechanismTyr/Ser + Lys bound to ribose-nicotinamide
  • D00274; UDP-glucose 4-epimerase
    (Catalytic domain: 3.40.50.720)
  • D00275; ADP-L-glycero-D-manno-heptose-6-epimerase
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • D00513; GDP-mannose 4,6 dehydratase 2
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • S00319; Alcohol dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00320; Acetoin(diacetyl) reductase
    (Catalytic domain: 3.40.50.720)
  • S00324; Glucose 1-dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00325; 3-alpha-hydroxysteroid dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00326; 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00327; Estradiol 17-beta-dehydrogenase 1
    (Catalytic domain: 3.40.50.720)
  • S00330; 7-alpha-hydroxysteroid dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00332; Tropinone reductase 2
    (Catalytic domain: 3.40.50.720)
  • S00552; Tropinone reductase 1
    (Catalytic domain: 3.40.50.720)
  • S00604; NADP-dependent mannitol dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00605; Sorbitol dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00608; 2-(R)-hydroxypropyl-CoM dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00610; 3-beta-hydroxysteroid dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00625; Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • 9.1050.440000.8011Hydride transferFrom CH-OH to nicotinamide (NAD+/NADP+)Drosophia alcohol dehydrogenase-like oxidation mechanismTyr/Thr + Lys bound to ribose-nicotinamide
  • D00262; dTDP-glucose 4,6-dehydratase 2
    (Catalytic domain: 3.40.50.720)
  • D00543; GDP-mannose 4,6-dehydratase
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • D00544; UDP-glucose 4-epimerase
    (Catalytic domain: 3.40.50.720)
  • 9.1050.584160.119Hydride transferFrom CH-OH to nicotinamide (NAD+/NADP+)GDP mannose dehydrogenase 3rd reaction step-like mechanismLys + Asp/Thr bound to water
  • T00227; UDP-glucose 6-dehydrogenase
    (Catalytic domains: 1.10.1040.10, 3.40.50.720)
  • T00408; GDP-mannose 6-dehydrogenase
    (Catalytic domains: 1.-.-.-, 3.40.50.720)
  • 9.1050.584160.160Hydride transferFrom CH-OH to nicotinamide (NAD+/NADP+)GDP mannose dehydrogenase 3rd reaction step-like mechanism[Asn or Gln] + mainchain amide group + Cys covalently-bound to substrate/intermediate
  • D00021; Betaine aldehyde dehydrogenase
    (Catalytic domains: 3.40.309.10, 3.40.605.10)
  • 9.5010.194230.8500Hydride transferFrom nicotinamide (NADH/NADPH) to C=O6-phospho-alpha-glucosidase 6th reaction step-like mechanismManganese ion bound to Cys/His/O7-nicotinamide/substrate or intermediate
  • D00827; Maltose-6''-phosphate glucosidase
    (Catalytic domain: 3.90.110.10)
  • 9.5010.536000.113Hydride transferFrom nicotinamide (NADH/NADPH) to C=OHMG-CoA reductase class II 3rd reaction step-like mechanismLys + two acidic residues
  • D00607; 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    (Catalytic domain: 3.90.770.10)
  • 9.5010.536000.976Hydride transferFrom nicotinamide (NADH/NADPH) to C=OHMG-CoA reductase class II 3rd reaction step-like mechanismTwo acidic residues + Lys (interacting with each other)
  • M00180; 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    (Catalytic domains: 3.30.70.420, 3.90.770.10)
  • 9.5010.536170.118Hydride transferFrom nicotinamide (NADH/NADPH) to C=OShikimate dehydrogenase-like reduction mechanismLys/Asp
  • D00605; Quinate/shikimate dehydrogenase
    (Catalytic domain: 3.40.192.10)
  • D00845; Shikimate dehydrogenase
    (Catalytic domain: 3.40.192.10)
  • D00857; Shikimate 5-dehydrogenase-like protein HI0607
    (Catalytic domains: 3.40.50.720, 3.40.192.10)
  • D00858; Quinate/shikimate dehydrogenase
    (Catalytic domain: 3.40.192.10)
  • T00414; Fusion: chorismate mutase and shikimate 5-dehydrogenase
    (Catalytic domain: 3.40.192.10)
  • 9.5010.536180.109Hydride transferFrom nicotinamide (NADH/NADPH) to C=OKetopantoate reductase-like reduction mechanismLys + an acidic residue + Asn or Gln + mainchain amide
  • D00603; 2-dehydropantoate 2-reductase
    (Catalytic domains: 1.10.1040.10, 3.40.50.720)
  • 9.5010.536200.8010Hydride transferFrom nicotinamide (NADH/NADPH) to C=OdTDP dehydrorhamnose reductase-like mechanismTyr/Ser + Lys bound to ribose-nicotinamide
  • D00274; UDP-glucose 4-epimerase
    (Catalytic domain: 3.40.50.720)
  • D00275; ADP-L-glycero-D-manno-heptose-6-epimerase
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • D00545; 3-oxoacyl-(Acyl-carrier protein) reductase
    (Catalytic domain: 3.40.50.720)
  • D00604; GDP-L-fucose synthetase
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • S00319; Alcohol dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00320; Acetoin(diacetyl) reductase
    (Catalytic domain: 3.40.50.720)
  • S00324; Glucose 1-dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00325; 3-alpha-hydroxysteroid dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00326; 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00327; Estradiol 17-beta-dehydrogenase 1
    (Catalytic domain: 3.40.50.720)
  • S00328; 3-oxoacyl-[acyl-carrier-protein] reductase
    (Catalytic domain: 3.40.50.720)
  • S00329; Sepiapterin reductase
    (Catalytic domain: 3.40.50.720)
  • S00331; Sniffer
    (Catalytic domain: 3.40.50.720)
  • S00332; Tropinone reductase 2
    (Catalytic domain: 3.40.50.720)
  • S00336; Tetrahydroxynaphthalene reductase
    (Catalytic domain: 3.40.50.720)
  • S00543; Carbonyl reductase [NADPH] 1
    (Catalytic domain: 3.40.50.720)
  • S00552; Tropinone reductase 1
    (Catalytic domain: 3.40.50.720)
  • S00553; 3-oxoacyl-[acyl carrier protein] reductase
    (Catalytic domain: 3.40.50.720)
  • S00602; L-xylulose reductase
    (Catalytic domain: 3.40.50.720)
  • S00604; NADP-dependent mannitol dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00605; Sorbitol dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00608; 2-(R)-hydroxypropyl-CoM dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • S00610; 3-beta-hydroxysteroid dehydrogenase
    (Catalytic domain: 3.40.50.720)
  • 9.5010.536200.8011Hydride transferFrom nicotinamide (NADH/NADPH) to C=OdTDP dehydrorhamnose reductase-like mechanismTyr/Thr + Lys bound to ribose-nicotinamide
  • D00544; UDP-glucose 4-epimerase
    (Catalytic domain: 3.40.50.720)
  • D00601; dTDP-4-dehydrorhamnose reductase
    (Catalytic domain: 3.40.50.720)
  • 9.5010.536210.8010Hydride transferFrom nicotinamide (NADH/NADPH) to C=OGDP mannose dehydratase-final reaction step-like mechanismTyr/Ser + Lys bound to ribose-nicotinamide
  • D00513; GDP-mannose 4,6 dehydratase 2
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • 9.5010.536210.8011Hydride transferFrom nicotinamide (NADH/NADPH) to C=OGDP mannose dehydratase-final reaction step-like mechanismTyr/Thr + Lys bound to ribose-nicotinamide
  • D00262; dTDP-glucose 4,6-dehydratase 2
    (Catalytic domain: 3.40.50.720)
  • D00543; GDP-mannose 4,6-dehydratase
    (Catalytic domains: 3.40.50.720, 3.90.25.10)
  • 9.5010.584100.113Hydride transferFrom nicotinamide (NADH/NADPH) to C=OHMG-CoA reductase class II 1st reaction step-like mechanismLys + two acidic residues
  • D00607; 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    (Catalytic domain: 3.90.770.10)
  • 9.5010.584100.976Hydride transferFrom nicotinamide (NADH/NADPH) to C=OHMG-CoA reductase class II 1st reaction step-like mechanismTwo acidic residues + Lys (interacting with each other)
  • M00180; 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    (Catalytic domains: 3.30.70.420, 3.90.770.10)
  • 10.12100.110.10080Electron transfer From dicopper (Cu-Cu) to Heme-FeIndirect transfer through protein residues + cofactor atoms; No covalent bond formation/cleavage mechanismHis bound to Cu-Cu + mainchain groups + Heme-acidic groups
  • M00062; Cytochrome c oxidase subunit 1-beta
    (Catalytic domains: 1.20.210.10, 2.60.40.420)
  • M00194; Cytochrome c oxidase subunit 1
    (Catalytic domains: 1.20.210.10, 2.60.40.420)
  • 10.21001.100.10900Electron transfer From [2Fe-2S] cluster to FeIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismAn acidic residue between His bound to [2Fe-2S] (bound to 2 x Cys/His cluster) + His bound to Fe3+ (bound to His/His/Asp cluster)
  • T00024; Naphthalene 1,2-dioxygenase subunit alpha
    (Catalytic domains: 2.102.10.10, 3.90.380.10)
  • 10.21011.100.10210Electron transfer From [2Fe-2S] cluster to diiron (Fe-Fe)Indirect transfer through protein residues; No covalent bond formation/cleavage mechanismTrp/Acidic residue + His bound to Fe-Fe (bound to Glu/His cluster)
  • S00028; Acyl-[acyl-carrier-protein] desaturase
    (Catalytic domain: 1.10.620.20)
  • 10.21021.100.10205Electron transfer From [2Fe-2S] cluster to [2Fe-2S] clusterIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismTrp + hydrophobic residue + Cys bound to [2Fe-2S] (bound to 2 x Cys/His cluster)
  • T00024; Naphthalene 1,2-dioxygenase subunit alpha
    (Catalytic domains: 2.102.10.10, 3.90.380.10)
  • 10.21022.100.10500Electron transfer From [2Fe-2S] cluster to [4Fe-4S] clusterIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismCys bound to [4Fe-4S] (bound to three Cys/His cluster)
  • M00042; Iron hydrogenase 1
    (Catalytic domains: 1.10.287.430, 3.10.20.30, 3.30.70.20, 3.40.50.1780, 3.40.950.10)
  • 10.21022.100.10510Electron transfer From [2Fe-2S] cluster to [4Fe-4S] clusterIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismCys bound to [2Fe-2S] (bound to four Cys cluster) + Cys bound to [4Fe-4S] (bound to four Cys cluster)
  • M00042; Iron hydrogenase 1
    (Catalytic domains: 1.10.287.430, 3.10.20.30, 3.30.70.20, 3.40.50.1780, 3.40.950.10)
  • 10.21100.110.10515Electron transfer From [2Fe-2S] cluster to Heme-FeIndirect transfer through protein residues + cofactor atoms; No covalent bond formation/cleavage mechanismCys bound to [2Fe-2S] (bound to four Cys cluster) + Acidic residue + Arg + Heme-acidic group
  • S00033; Camphor 5-monooxygenase
    (Catalytic domain: 1.10.630.10)
  • 10.22022.100.10700Electron transfer From [4Fe-4S] cluster to [4Fe-4S] clusterIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismHydrophobic residue between Cys/His bound to [4Fe-4S] (bound to three Cys/His cluster) + Cys/Cys bound to [4Fe-4S] (bound to four Cys cluster)
  • M00042; Iron hydrogenase 1
    (Catalytic domains: 1.10.287.430, 3.10.20.30, 3.30.70.20, 3.40.50.1780, 3.40.950.10)
  • 10.22022.100.10750Electron transfer From [4Fe-4S] cluster to [4Fe-4S] clusterIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismMet between Cys residues bound to two [4Fe-4S] clusters (bound to four Cys clusters)
  • M00042; Iron hydrogenase 1
    (Catalytic domains: 1.10.287.430, 3.10.20.30, 3.30.70.20, 3.40.50.1780, 3.40.950.10)
  • 10.22022.100.10751Electron transfer From [4Fe-4S] cluster to [4Fe-4S] clusterIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismHydrophobic residue between Cys residues bound to two [4Fe-4S] clusters (bound to four Cys clusters)
  • M00012; Periplasmic [Fe] hydrogenase large subunit
    (Catalytic domains: 3.40.50.1780, 3.40.950.10)
  • 10.22023.100.10525Electron transfer From [4Fe-4S] cluster to [3Fe-4S] clusterIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismCys bound to [4Fe-4S] (bound to three Cys/His cluster) + Cys bound to [3Fe-4S] (bound to three Cys cluster)
  • T00030; Periplasmic [NiFe] hydrogenase large subunit
    (Catalytic domains: 1.10.645.10, 3.40.50.700, 4.10.480.10)
  • 10.22025.100.10760Electron transfer From [4Fe-4S] cluster to [8Fe-7S] cluster (P cluster)Indirect transfer through protein residues; No covalent bond formation/cleavage mechanismHydrophobic residue between Cys bound to [4Fe-4S] (bound to four Cys cluster) + Cys bound to [8Fe-7S] (P cluster) (bound to six Cys/Ser cluster)
  • M00114; Nitrogenase molybdenum-iron protein alpha chain
    (Catalytic domains: 3.40.50.300, 3.40.50.1980)
  • 10.22025.100.10765Electron transfer From [4Fe-4S] cluster to [8Fe-7S] cluster (P cluster)Indirect transfer through protein residues; No covalent bond formation/cleavage mechanismThr (or Ser) between Cys bound to [4Fe-4S] (bound to four Cys cluster) + Cys bound to [8Fe-7S] (P cluster) (bound to six Cys/Ser cluster)
  • M00199; Nitrogenase molybdenum-iron protein alpha chain
    (Catalytic domains: 3.40.50.300, 3.40.50.1980)
  • 10.22100.110.10570Electron transfer From [4Fe-4S] cluster to Heme-FeIndirect transfer through protein residues + cofactor atoms; No covalent bond formation/cleavage mechanismCys bound to [4Fe-4S] (bound to four Cys cluster) + Cys covalently bonded to Heme group + Heme-vinyl group
  • M00012; Periplasmic [Fe] hydrogenase large subunit
    (Catalytic domains: 3.40.50.1780, 3.40.950.10)
  • 10.22120.100.10610Electron transfer From [4Fe-4S] cluster to H cluster ([4Fe-4S]/diiron/bridging cys)Indirect transfer through protein residues; No covalent bond formation/cleavage mechanismGln between Cys bound to [4Fe-4S] (bound to four Cys cluster) + Cys bound to H cluster (bound to four Cys cluster)
  • M00042; Iron hydrogenase 1
    (Catalytic domains: 1.10.287.430, 3.10.20.30, 3.30.70.20, 3.40.50.1780, 3.40.950.10)
  • 10.22140.100.10551Electron transfer From [4Fe-4S] cluster to Ni-Fe(CN)2(CO)Indirect transfer through protein residues; No covalent bond formation/cleavage mechanismCys bound to [4Fe-4S] (bound to four Cys cluster) + Cys bound to Ni-Fe(CN)2(CO) (bound to four Cys cluster)
  • T00030; Periplasmic [NiFe] hydrogenase large subunit
    (Catalytic domains: 1.10.645.10, 3.40.50.700, 4.10.480.10)
  • 10.23022.100.10580Electron transfer From [3Fe-4S] cluster to [4Fe-4S] clusterIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismCys bound to [3Fe-4S] (bound to three Cys cluster) + Cys bound to [4Fe-4S] (bound to four Cys cluster)
  • T00030; Periplasmic [NiFe] hydrogenase large subunit
    (Catalytic domains: 1.10.645.10, 3.40.50.700, 4.10.480.10)
  • 10.25130.110.10590Electron transfer From [8Fe-7S] cluster (P cluster) to [Mo-7Fe-9S] cluster + homocitrate (FeMoco)Indirect transfer through protein residues + cofactor atoms; No covalent bond formation/cleavage mechanismCys bound to [8Fe-7S] (P cluster) (bound to six Cys/Ser cluster) + Gln bound to homocitrate (bound to Gln/His/Ile + FeMoco (bound to His/Cys))
  • M00114; Nitrogenase molybdenum-iron protein alpha chain
    (Catalytic domains: 3.40.50.300, 3.40.50.1980)
  • M00199; Nitrogenase molybdenum-iron protein alpha chain
    (Catalytic domains: 3.40.50.300, 3.40.50.1980)
  • 10.100012.100.10200Electron transfer From Heme-Fe to dicopper (Cu-Cu)Indirect transfer through protein residues; No covalent bond formation/cleavage mechanismTrp + Met bound to Cu-Cu (bound to Cys/His/Met/Glu cluster)
  • M00062; Cytochrome c oxidase subunit 1-beta
    (Catalytic domains: 1.20.210.10, 2.60.40.420)
  • 10.100012.100.10290Electron transfer From Heme-Fe to dicopper (Cu-Cu)Indirect transfer through protein residues; No covalent bond formation/cleavage mechanismMainchain atoms + Met bound to Cu-Cu (bound to Cys/His/Met/Gln cluster)
  • M00194; Cytochrome c oxidase subunit 1
    (Catalytic domains: 1.20.210.10, 2.60.40.420)
  • 10.100110.100.10300Electron transfer From Heme-Fe to Heme-Fe/CuIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismMainchain atoms between two His residues (axial ligands) bound to (Heme-Fe)2
  • M00062; Cytochrome c oxidase subunit 1-beta
    (Catalytic domains: 1.20.210.10, 2.60.40.420)
  • M00194; Cytochrome c oxidase subunit 1
    (Catalytic domains: 1.20.210.10, 2.60.40.420)
  • 10.120022.100.10610Electron transfer From H cluster ([4Fe-4S]/diiron/bridging cys) to [4Fe-4S] clusterIndirect transfer through protein residues; No covalent bond formation/cleavage mechanismGln between Cys bound to [4Fe-4S] (bound to four Cys cluster) + Cys bound to H cluster (bound to four Cys cluster)
  • M00012; Periplasmic [Fe] hydrogenase large subunit
    (Catalytic domains: 3.40.50.1780, 3.40.950.10)
  • 10.400410.30292300.20000Electron transfer From sulfhydryl to disulfideDirect transfer through thiol-disulfide exchange (Nucleophile); Nucleophile attacks on target disulfide bond; New bond formation and cleavage accompanied by electron transfer mechanismCys + Cys-Cys(disulfide bond)

    Copyright: Nozomi Nagano, JST & CBRC-AIST
    Funded by PRESTO/Japan Science and Technology Agency (JST) (December 2001 - November 2004)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
    Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
    Funded by BIRD/Japan Science and Technology Agency (JST) (September 2005 - September 2008)
    Funded by BIRD/Japan Science and Technology Agency (JST) (October 2007 - September 2010)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2012 - March 2013)
    Supported by the commission for the Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI) (October 2012 - March 2016)
    Funded by the project commissioned by the New Energy and Industrial Technology Development Organization (NEDO) (April 2016 -)