EzCatDB: D00027
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DB codeD00027
CATH domainDomain 13.40.50.720 : Rossmann foldCatalytic domain
Domain 23.30.360.10 : Dihydrodipicolinate Reductase; domain 2
E.C.1.3.1.24
CSA1gcu

CATH domainRelated DB codes (homologues)
3.30.360.10 : Dihydrodipicolinate Reductase; domain 2T00219,D00003,D00010,D00017,D00023,D00028,D00034,D00476
3.40.50.720 : Rossmann foldS00543,S00551,S00552,S00553,S00602,S00604,S00605,S00608,S00610,S00625,S00319,S00328,S00329,S00330,S00331,S00332,D00456,D00457,D00458,S00324,S00320,S00325,S00326,S00327,D00459,S00335,S00336,S00334,T00219,S00339,D00513,D00001,D00002,D00003,D00005,D00007,D00008,D00010,D00012,D00017,D00018,D00023,D00028,D00031,D00032,D00033,D00034,D00035,D00037,D00048,D00071,D00476,D00481,D00482,D00490,D00492,D00494,D00545,D00601,D00603,D00604,D00605,D00615,D00845,D00857,D00858,M00161,M00171,M00210,T00002,T00010,T00011,T00015,T00227,T00247,T00408,T00414,D00827,D00262,D00274,D00275,M00035,T00109

Enzyme Name
UniProtKBKEGG

P46844
Protein nameBiliverdin reductase Abiliverdin reductase
SynonymsBVR A
EC 1.3.1.24
Biliverdin-IX alpha-reductase
RefSeqNP_446302.1 (Protein)
NM_053850.1 (DNA/RNA sequence)
PfamPF09166 (Biliv-reduc_cat)
PF01408 (GFO_IDH_MocA)
[Graphical view]

KEGG pathways
MAP codePathways
MAP00860Porphyrin and chlorophyll metabolism

UniProtKB:Accession NumberP46844
Entry nameBIEA_RAT
ActivityBilirubin + NAD(P)(+) = biliverdin + NAD(P)H.
SubunitMonomer (By similarity).
Subcellular locationCytoplasm.
CofactorBinds 1 zinc ion per subunit.

Compound table: links to PDB-related databases & PoSSuM

CofactorsSubstratesProducts
KEGG-idC00038C00486C00003C00006C00500C00004C00005C00080
CompoundZincBilirubinNAD+NADP+BiliverdinNADHNADPHH+
Typeheavy metalamide group,aromatic ring (with nitrogen atoms),carboxyl groupamide group,amine group,nucleotideamide group,amine group,nucleotideamide group,aromatic ring (with nitrogen atoms),carboxyl groupamide group,amine group,nucleotideamide group,amine group,nucleotideothers
ChEBI29105
16990
15846
18009
17033
16908
16474
15378
PubChem32051
5280352
5893
5886

439153
5884
1038
                
1gcuA01UnboundUnboundUnboundUnboundUnboundUnboundUnbound 
1lc0A01UnboundUnboundUnboundUnboundUnboundUnboundUnbound 
1lc3A01UnboundUnboundBound:NADUnboundUnboundUnboundUnbound 
1gcuA02UnboundUnboundUnboundUnboundUnboundUnboundUnbound 
1lc0A02UnboundUnboundUnboundUnboundUnboundUnboundUnbound 
1lc3A02UnboundUnboundUnboundUnboundUnboundUnboundUnbound 

Active-site residues
resource
Swiss-prot & literature [5] & [8]
pdbCatalytic residuesMain-chain involved in catalysiscomment
           
1gcuA01TYR 97
 
 
1lc0A01TYR 97
 
 
1lc3A01TYR 97
 
 
1gcuA02 
HIS 279;CYS 280;CYS 291;HIS 292(Zinc binding)
 
1lc0A02 
HIS 279;CYS 280;CYS 291;       (Zinc binding)
invisible H292-
1lc3A02 
HIS 279;CYS 280;CYS 291;HIS 292(Zinc binding)
 

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[5]p.224
[6]p.217
[7]Fig.4
[8]Fig.8

references
[1]AIST-OJ
PubMed ID8253726
JournalJ Biol Chem
Year1993
Volume268
Pages26099-106
AuthorsTerry MJ, Maines MD, Lagarias JC
TitleInactivation of phytochrome- and phycobiliprotein-chromophore precursors by rat liver biliverdin reductase.
[2]
CommentsMUTAGENESIS
Medline ID94291657
PubMed ID8020496
JournalEur J Biochem
Year1994
Volume222
Pages597-603
AuthorsMcCoubrey WK Jr, Maines MD
TitleSite-directed mutagenesis of cysteine residues in biliverdin reductase. Roles in substrate and cofactor binding.
Related UniProtKBP46844
[3]
PubMed ID9359830
JournalBiochem J
Year1997
Volume328
Pages33-6
AuthorsEnnis O, Maytum R, Mantle TJ
TitleCloning and overexpression of rat kidney biliverdin IX alpha reductase as a fusion protein with glutathione S-transferase: stereochemistry of NADH oxidation and evidence that the presence of the glutathione S-transferase domain does not effect BVR-A activity.
[4]
CommentsX-ray crystallography
PubMed ID10957639
JournalActa Crystallogr D Biol Crystallogr
Year2000
Volume56
Pages1180-2
AuthorsSun D, Sato M, Yoshida T, Shimizu H, Miyatake H, Adachi S, Shiro Y, Kikuchi A
TitleCrystallization and preliminary X-ray diffraction analysis of a rat biliverdin reductase.
Related PDB1gcu
[5]
PubMed ID11224565
JournalNat Struct Biol
Year2001
Volume8
Pages221-5
AuthorsKikuchi A, Park SY, Miyatake H, Sun D, Sato M, Yoshida T, Shiro Y
TitleCrystal structure of rat biliverdin reductase.
[6]
PubMed ID11224564
JournalNat Struct Biol
Year2001
Volume8
Pages215-20
AuthorsPereira PJ, Macedo-Ribeiro S, Parraga A, Perez-Luque R, Cunningham O, Darcy K, Mantle TJ, Coll M
TitleStructure of human biliverdin IXbeta reductase, an early fetal bilirubin IXbeta producing enzyme.
[7]
PubMed ID11224558
JournalNat Struct Biol
Year2001
Volume8
Pages198-200
AuthorsMcDonagh AF
TitleTurning green to gold.
[8]
PubMed ID12079357
JournalJ Mol Biol
Year2002
Volume319
Pages1199-210
AuthorsWhitby FG, Phillips JD, Hill CP, McCoubrey W, Maines MD
TitleCrystal structure of a biliverdin IXalpha reductase enzyme-cofactor complex.
Related PDB1lc0,1lc3


createdupdated
2004-03-162009-02-26


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