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CATH domain | Related DB codes (homologues) |
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3.30.9.10 : D-Amino Acid Oxidase; Chain A, domain 2 | D00037,D00064,D00494,T00025 | 3.50.50.60 : FAD/NAD(P)-binding domain | M00163,D00015,D00042,D00045,D00064,D00071,T00004,T00015,T00017,T00025,T00211,T00213,T00233,T00242 |
KEGG pathways | MAP code | Pathways |
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MAP00260 | Glycine, serine and threonine metabolism |
UniProtKB:Accession Number | P40859 |
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Entry name | MSOX_BACB0 |
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Activity | Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2). |
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Subunit | Monomer. |
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Subcellular location | Cytoplasm. |
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Cofactor | Binds 1 FAD per subunit. |
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References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
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[10] | Fig.6, p.339 |
| [12] | Scheme 1 |
| [15] |
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| [17] | Scheme 2 |
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references | [1] |
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PubMed ID | 3427080 |
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Journal | Biochemistry |
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Year | 1987 |
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Volume | 26 |
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Pages | 7391-5 |
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Authors | Kvalnes-Krick K, Jorns MS |
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Title | Interaction of tetrahydrofolate and other folate derivatives with bacterial sarcosine oxidase. |
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[2] |
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PubMed ID | 1657156 |
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Journal | Biochemistry |
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Year | 1991 |
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Volume | 30 |
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Pages | 10980-6 |
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Authors | Ali SN, Zeller HD, Calisto MK, Jorns MS |
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Title | Kinetics of electron entry, exit, and interflavin electron transfer during catalysis by sarcosine oxidase. |
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[3] |
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PubMed ID | 1939012 |
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Journal | J Biochem (Tokyo) |
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Year | 1991 |
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Volume | 109 |
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Pages | 909-17 |
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Authors | Suzuki H, Kawamura-Konishi Y |
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Title | Cysteine residues in the active site of Corynebacterium sarcosine oxidase. |
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[4] |
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PubMed ID | 7887617 |
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Journal | Appl Environ Microbiol |
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Year | 1995 |
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Volume | 61 |
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Pages | 367-70 |
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Authors | Nishiya Y, Zuihara S, Imanaka T |
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Title | Active site analysis and stabilization of sarcosine oxidase by the substitution of cysteine residues. |
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[5] |
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PubMed ID | 8779579 |
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Journal | Appl Environ Microbiol |
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Year | 1996 |
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Volume | 62 |
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Pages | 2405-10 |
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Authors | Nishiya Y, Imanaka T |
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Title | Analysis of interaction between the Arthrobacter sarcosine oxidase and the coenzyme flavin adenine dinucleotide by site-directed mutagenesis. |
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[6] |
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PubMed ID | 8611516 |
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Journal | Biochemistry |
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Year | 1996 |
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Volume | 35 |
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Pages | 5292-9 |
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Authors | Willie A, Edmondson DE, Jorns MS |
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Title | Sarcosine oxidase contains a novel covalently bound FMN. |
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[7] |
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PubMed ID | 9356299 |
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Journal | J Struct Biol |
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Year | 1997 |
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Volume | 120 |
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Pages | 109-11 |
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Authors | Ichikawa T, Sasaki H, Koike H, Nishiyama M, Koyama Y, Tanokura M |
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Title | Crystallization and preliminary crystallographic analysis of the sarcosine oxidase from Bacillus sp. NS-129. |
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[8] |
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PubMed ID | 9485355 |
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Journal | Biochemistry |
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Year | 1998 |
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Volume | 37 |
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Pages | 2089-95 |
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Authors | Chlumsky LJ, Sturgess AW, Nieves E, Jorns MS |
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Title | Identification of the covalent flavin attachment site in sarcosine oxidase. |
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[9] |
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Comments | CHARACTERIZATION, SEQUENCE OF 313-319, AND MASS SPECTROMETRY |
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Medline ID | 99238335 |
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PubMed ID | 10220347 |
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Journal | Biochemistry |
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Year | 1999 |
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Volume | 38 |
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Pages | 5588-95 |
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Authors | Wagner MA, Khanna P, Jorns MS |
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Title | Structure of the flavocoenzyme of two homologous amine oxidases: monomeric sarcosine oxidase and N-methyltryptophan oxidase. |
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Related UniProtKB | P40859 |
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[10] |
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Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) |
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Medline ID | 99197298 |
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PubMed ID | 10368302 |
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Journal | Structure Fold Des |
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Year | 1999 |
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Volume | 7 |
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Pages | 331-45 |
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Authors | Trickey P, Wagner MA, Jorns MS, Mathews FS |
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Title | Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme. |
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Related PDB | 1b3m,1l9f |
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Related UniProtKB | P40859 |
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[11] |
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Comments | CHARACTERIZATION |
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Medline ID | 20374561 |
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PubMed ID | 10913293 |
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Journal | Biochemistry |
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Year | 2000 |
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Volume | 39 |
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Pages | 8825-9 |
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Authors | Wagner MA, Jorns MS |
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Title | Monomeric sarcosine oxidase: 2. Kinetic studies with sarcosine, alternate substrates, and a substrate analogue. |
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Related UniProtKB | P40859 |
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[12] |
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Comments | X-ray crystallography |
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PubMed ID | 10913292 |
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Journal | Biochemistry |
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Year | 2000 |
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Volume | 39 |
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Pages | 8813-24 |
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Authors | Wagner MA, Trickey P, Chen ZW, Mathews FS, Jorns MS |
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Title | Monomeric sarcosine oxidase: 1. Flavin reactivity and active site binding determinants. |
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Related PDB | 1el5,1el7,1el8,1el9,1eli |
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[13] |
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Comments | CHARACTERIZATION |
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Medline ID | 20541388 |
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PubMed ID | 11087383 |
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Journal | Biochemistry |
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Year | 2000 |
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Volume | 39 |
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Pages | 14341-7 |
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Authors | Zhao G, Qu J, Davis FA, Jorns MS |
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Title | Inactivation of monomeric sarcosine oxidase by reaction with N-(cyclopropyl)glycine. |
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[14] |
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PubMed ID | 11035956 |
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Journal | Protein Expr Purif |
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Year | 2000 |
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Volume | 20 |
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Pages | 95-7 |
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Authors | Nishiya Y |
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Title | A mutant sarcosine oxidase in which activity depends on flavin adenine dinucleotide. |
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[15] |
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Comments | X-ray crystallography |
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PubMed ID | 12146941 |
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Journal | Biochemistry |
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Year | 2002 |
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Volume | 41 |
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Pages | 9751-64 |
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Authors | Zhao G, Song H, Chen ZW, Mathews FS, Jorns MS |
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Title | Monomeric sarcosine oxidase: role of histidine 269 in catalysis. |
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Related PDB | 1l9c,1l9d,1l9e |
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[16] |
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PubMed ID | 12084049 |
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Journal | Eur J Biochem |
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Year | 2002 |
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Volume | 269 |
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Pages | 3096-102 |
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Authors | Sutcliffe MJ, Scrutton NS |
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Title | A new conceptual framework for enzyme catalysis. Hydrogen tunnelling coupled to enzyme dynamics in flavoprotein and quinoprotein enzymes. |
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[17] |
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PubMed ID | 12549903 |
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Journal | Biochemistry |
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Year | 2003 |
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Volume | 42 |
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Pages | 864-9 |
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Authors | Khanna P, Jorns MS |
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Title | Tautomeric rearrangement of a dihydroflavin bound to monomeric sarcosine oxidase or N-methyltryptophan oxidase. |
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[18] |
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PubMed ID | 15240125 |
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Journal | Biochem Biophys Res Commun |
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Year | 2004 |
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Volume | 320 |
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Pages | 846-51 |
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Authors | Mukouyama EB, Oguchi M, Kodera Y, Maeda T, Suzuki H |
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Title | Low pKa lysine residues at the active site of sarcosine oxidase from Corynebacterium sp. U-96. |
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comments | This enzyme catalyzes several reactions as follows: (A) Oxidation of sarcosine by FAD, giving a Schiff-base intermediate and FADH2. (B) Exchange of double-bonded atoms (Schiff-base) by hydration, giving formaldehyde and glycine. (C) Hydride transfer from FADH2 to O2 (Re-oxidation of FAD by O2, releasing H2O2).
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created | updated |
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2004-03-24 | 2009-02-26 |
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