|
CATH domain | Related DB codes (homologues) |
---|
3.30.63.10 : Guanylate Kinase phosphate binding domain | D00540 | 3.40.50.300 : Rossmann fold | S00527,S00547,S00548,S00550,S00554,S00555,S00671,S00672,S00676,S00680,S00682,S00913,S00914,S00301,S00302,S00303,S00304,S00307,S00308,S00305,S00306,S00309,S00310,S00311,M00114,M00199,D00130,D00540,M00186 |
KEGG pathways | MAP code | Pathways |
---|
MAP00230 | Purine metabolism |
UniProtKB:Accession Number | P15454 | Q8I2M1 | Q64520 |
---|
Entry name | KGUA_YEAST | Q8I2M1_PLAF7 | KGUA_MOUSE |
---|
Activity | ATP + GMP = ADP + GDP. |
| ATP + GMP = ADP + GDP. |
---|
Subunit | Monomer. |
| Monomer (By similarity). |
---|
Subcellular location |
|
|
|
---|
Cofactor |
|
|
|
---|
Compound table: links to PDB-related databases & PoSSuM |
---|
| Cofactors | Substrates | Products |
---|
KEGG-id | C00305 | C00002 | C00144 | C00008 | C00035 |
---|
Compound | Magnesium | ATP | GMP | ADP | GDP |
---|
Type | divalent metal (Ca2+, Mg2+) | amine group,nucleotide | amide group,amine group,nucleotide | amine group,nucleotide | amide group,amine group,nucleotide |
---|
ChEBI | 18420
| 15422
| 17345
| 16761
| 17552
|
---|
PubChem | 888
| 5957
| 6804
| 6022
| 8977
|
---|
| | | | | | | | | | | | |
---|
1ex6A01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1ex6B01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1ex7A01 |  |  |  |  |  |  |  | Unbound | Unbound | Bound:5GP | Unbound | Unbound |
---|
1gkyA01 |  |  |  |  |  |  |  | Unbound | Unbound | Bound:5GP | Unbound | Unbound |
---|
1z6gA01 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1lvgA01 |  |  |  |  |  |  |  | Unbound | Unbound | Bound:5GP | Unbound | Unbound |
---|
1ex6A02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1ex6B02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1ex7A02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1gkyA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1z6gA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
1lvgA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | Bound:ADP | Unbound |
---|
References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
---|
[3] | p.1140 |
| [6] | p.110-119 |
| [9] | p.30241 |
|
references | [1] |
---|
PubMed ID | 2551688 |
---|
Journal | Eur J Biochem |
---|
Year | 1989 |
---|
Volume | 184 |
---|
Pages | 433-43 |
---|
Authors | Berger A, Schiltz E, Schulz GE |
---|
Title | Guanylate kinase from Saccharomyces cerevisiae. Isolation and characterization, crystallization and preliminary X-ray analysis, amino acid sequence and comparison with adenylate kinases. |
---|
[2] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). |
---|
Medline ID | 90133954 |
---|
PubMed ID | 1967656 |
---|
Journal | J Mol Biol |
---|
Year | 1990 |
---|
Volume | 211 |
---|
Pages | 249-54 |
---|
Authors | Stehle T, Schulz GE |
---|
Title | Three-dimensional structure of the complex of guanylate kinase from yeast with its substrate GMP. |
---|
Related UniProtKB | P15454 |
---|
[3] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). |
---|
Medline ID | 92235848 |
---|
PubMed ID | 1314905 |
---|
Journal | J Mol Biol |
---|
Year | 1992 |
---|
Volume | 224 |
---|
Pages | 1127-41 |
---|
Authors | Stehle T, Schulz GE |
---|
Title | Refined structure of the complex between guanylate kinase and its substrate GMP at 2.0 A resolution. |
---|
Related PDB | 1gky |
---|
Related UniProtKB | P15454 |
---|
[4] |
---|
PubMed ID | 8910414 |
---|
Journal | J Biol Chem |
---|
Year | 1996 |
---|
Volume | 271 |
---|
Pages | 28038-44 |
---|
Authors | Li Y, Zhang Y, Yan H |
---|
Title | Kinetic and thermodynamic characterizations of yeast guanylate kinase. |
---|
[5] |
---|
PubMed ID | 9235932 |
---|
Journal | J Biol Chem |
---|
Year | 1997 |
---|
Volume | 272 |
---|
Pages | 19343-50 |
---|
Authors | Zhang Y, Li Y, Wu Y, Yan H |
---|
Title | Structural and functional roles of tyrosine 78 of yeast guanylate kinase. |
---|
[6] |
---|
PubMed ID | 10218107 |
---|
Journal | Adv Enzymol Relat Areas Mol Biol |
---|
Year | 1999 |
---|
Volume | 73 |
---|
Pages | 103-34 |
---|
Authors | Yan H, Tsai MD |
---|
Title | Nucleoside monophosphate kinases: structure, mechanism, and substrate specificity. |
---|
[7] |
---|
PubMed ID | 10939525 |
---|
Journal | Mol Biol Rep |
---|
Year | 2000 |
---|
Volume | 27 |
---|
Pages | 45-9 |
---|
Authors | Kumar V |
---|
Title | Cloning and sequence analysis of lily and tobacco guanylate kinases. |
---|
[8] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 11243817 |
---|
Journal | J Mol Biol |
---|
Year | 2001 |
---|
Volume | 307 |
---|
Pages | 247-57 |
---|
Authors | Blaszczyk J, Li Y, Yan H, Ji X |
---|
Title | Crystal structure of unligated guanylate kinase from yeast reveals GMP-induced conformational changes. |
---|
Related PDB | 1ex6,1ex7 |
---|
[9] |
---|
PubMed ID | 12036965 |
---|
Journal | J Biol Chem |
---|
Year | 2002 |
---|
Volume | 277 |
---|
Pages | 30236-43 |
---|
Authors | Sekulic N, Shuvalova L, Spangenberg O, Konrad M, Lavie A |
---|
Title | Structural characterization of the closed conformation of mouse guanylate kinase. |
---|
Related PDB | 1lvg |
---|
comments | This enzyme is homologous to the counterpart enzyme from bacteria (D00540 in EzCatDB). Although the catalytic mechanism of this enzyme must be similar to that of adenylate kinase (S00305 in EzCatDB), according to the literature [3] & [6], the catalytic residues are a little bit different. Along with basic residues (Lys14, Arg38, Arg41, Arg135 & Arg146 of 1ex7), tyrosine residues (Tyr50 & Tyr78) act as stabilizers for the transition state. Although magnesium bound structures have not been reported for this enzyme, Asp98 must play a role in binding of magnesium ion. Moreover, the literature [6] supported the associative mechanism (or SN2 mechanism).
|
created | updated |
---|
2004-03-18 | 2009-04-08 |
|
|