|
UniProtKB:Accession Number | P60484 |
---|
Entry name | PTEN_HUMAN |
---|
Activity | Phosphatidylinositol 3,4,5-trisphosphate + H(2)O = phosphatidylinositol 4,5-bisphosphate + phosphate.,A phosphoprotein + H(2)O = a protein + phosphate.,Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate. |
---|
Subunit | Monomer. The unphosphorylated form interacts with the second PDZ domain of AIP1 and with DLG1 and MAST2 in vitro. Interacts with MAGI3. |
---|
Subcellular location | Cytoplasm. |
---|
Cofactor | Magnesium. |
---|
Compound table: links to PDB-related databases & PoSSuM |
---|
| Cofactors | Substrates | Products |
---|
KEGG-id | C00305 | C00001 | C05981 | C00562 | C01167 | C00009 | C04637 | C00017 | C00585 |
---|
E.C. |
| 3.1.3.67,3.1.3.16,3.1.3.48 | 3.1.3.67 | 3.1.3.16 | 3.1.3.48 | 3.1.3.67,3.1.3.16,3.1.3.48 | 3.1.3.67 | 3.1.3.16 | 3.1.3.48 |
---|
Compound | Magnesium | H2O | Phosphatidylinositol-3,4,5-trisphosphate | Phosphoprotein | Protein tyrosine phosphate | Orthophosphate | Phosphatidylinositol 4,5-bisphosphate | Protein | Protein tyrosine |
---|
Type | divalent metal (Ca2+, Mg2+) | H2O | carbohydrate,lipid,phosphate group/phosphate ion | peptide/protein,phosphate group/phosphate ion | aromatic ring (only carbon atom),peptide/protein,phosphate group/phosphate ion | phosphate group/phosphate ion | carbohydrate,lipid,phosphate group/phosphate ion | peptide/protein | aromatic ring (only carbon atom),peptide/protein |
---|
ChEBI | 18420
| 15377
|
|
|
| 26078
|
|
|
|
---|
PubChem | 888
| 22247451 962
|
|
|
| 1004 22486802
|
|
|
|
---|
| | | | | | | | | | | | | | | | |
---|
1d5rA01 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Analogue:TLA | Unbound | Unbound |
---|
1d5rA02 |  |  |  |  |  |  |  | Unbound | | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
---|
References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
---|
[4] | p.326 |
|
references | [1] |
---|
PubMed ID | 9399917 |
---|
Journal | Am J Hum Genet |
---|
Year | 1997 |
---|
Volume | 61 |
---|
Pages | 1234-8 |
---|
Authors | Myers MP, Tonks NK |
---|
Title | PTEN: sometimes taking it off can be better than putting it on. |
---|
[2] |
---|
PubMed ID | 9367992 |
---|
Journal | J Biol Chem |
---|
Year | 1997 |
---|
Volume | 272 |
---|
Pages | 29403-6 |
---|
Authors | Li L, Ernsting BR, Wishart MJ, Lohse DL, Dixon JE |
---|
Title | A family of putative tumor suppressors is structurally and functionally conserved in humans and yeast. |
---|
[3] |
---|
Comments | CHARACTERIZATION |
---|
Medline ID | 97404346 |
---|
PubMed ID | 9256433 |
---|
Journal | Proc Natl Acad Sci U S A |
---|
Year | 1997 |
---|
Volume | 94 |
---|
Pages | 9052-7 |
---|
Authors | Myers MP, Stolarov JP, Eng C, Li J, Wang SI, Wigler MH, Parsons R, Tonks NK |
---|
Title | P-TEN, the tumor suppressor from human chromosome 10q23, is a dual-specificity phosphatase. |
---|
Related UniProtKB | O00633 |
---|
[4] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 10555148 |
---|
Journal | Cell |
---|
Year | 1999 |
---|
Volume | 99 |
---|
Pages | 323-34 |
---|
Authors | Lee JO, Yang H, Georgescu MM, Di Cristofano A, Maehama T, Shi Y, Dixon JE, Pandolfi P, Pavletich NP |
---|
Title | Crystal structure of the PTEN tumor suppressor: implications for its phosphoinositide phosphatase activity and membrane association. |
---|
Related PDB | 1d5r |
---|
[5] |
---|
PubMed ID | 10617421 |
---|
Journal | Science |
---|
Year | 1999 |
---|
Volume | 286 |
---|
Pages | 2096-7 |
---|
Authors | Tonks NK, Myers MP |
---|
Title | Structural assets of a tumor suppressor. |
---|
[6] |
---|
PubMed ID | 10698713 |
---|
Journal | Biochem J |
---|
Year | 2000 |
---|
Volume | 346 Pt 3 |
---|
Pages | 827-33 |
---|
Authors | Leslie NR, Gray A, Pass I, Orchiston EA, Downes CP |
---|
Title | Analysis of the cellular functions of PTEN using catalytic domain and C-terminal mutations: differential effects of C-terminal deletion on signalling pathways downstream of phosphoinositide 3-kinase. |
---|
[7] |
---|
PubMed ID | 10656991 |
---|
Journal | Biochim Biophys Acta |
---|
Year | 2000 |
---|
Volume | 1470 |
---|
Pages | R17-20 |
---|
Authors | Roussel MF |
---|
Title | The Cold Spring Harbor Laboratory meeting on tyrosine phosphorylation and cell signaling. Cold Spring Harbor, NY, USA, May 12-16, 1999. |
---|
[8] |
---|
PubMed ID | 11156408 |
---|
Journal | Cancer Res |
---|
Year | 2000 |
---|
Volume | 60 |
---|
Pages | 7033-8 |
---|
Authors | Georgescu MM, Kirsch KH, Kaloudis P, Yang H, Pavletich NP, Hanafusa H |
---|
Title | Stabilization and productive positioning roles of the C2 domain of PTEN tumor suppressor. |
---|
[9] |
---|
PubMed ID | 11709086 |
---|
Journal | Biochem Soc Trans |
---|
Year | 2001 |
---|
Volume | 29 |
---|
Pages | 846-51 |
---|
Authors | Downes CP, Bennett D, McConnachie G, Leslie NR, Pass I, MacPhee C, Patel L, Gray A |
---|
Title | Antagonism of PI 3-kinase-dependent signalling pathways by the tumour suppressor protein, PTEN. |
---|
[10] |
---|
PubMed ID | 11431330 |
---|
Journal | Cancer Res |
---|
Year | 2001 |
---|
Volume | 61 |
---|
Pages | 4985-9 |
---|
Authors | Tolkacheva T, Boddapati M, Sanfiz A, Tsuchida K, Kimmelman AC, Chan AM |
---|
Title | Regulation of PTEN binding to MAGI-2 by two putative phosphorylation sites at threonine 382 and 383. |
---|
[11] |
---|
PubMed ID | 11237521 |
---|
Journal | Exp Cell Res |
---|
Year | 2001 |
---|
Volume | 264 |
---|
Pages | 29-41 |
---|
Authors | Simpson L, Parsons R |
---|
Title | PTEN: life as a tumor suppressor. |
---|
[12] |
---|
PubMed ID | 11810268 |
---|
Journal | Hum Genet |
---|
Year | 2001 |
---|
Volume | 109 |
---|
Pages | 569-75 |
---|
Authors | Guipponi M, Tapparel C, Jousson O, Scamuffa N, Mas C, Rossier C, Hutter P, Meda P, Lyle R, Reymond A, Antonarakis SE |
---|
Title | The murine orthologue of the Golgi-localized TPTE protein provides clues to the evolutionary history of the human TPTE gene family. |
---|
[13] |
---|
PubMed ID | 11279206 |
---|
Journal | J Biol Chem |
---|
Year | 2001 |
---|
Volume | 276 |
---|
Pages | 21745-53 |
---|
Authors | Wu Y, Dowbenko D, Pisabarro MT, Dillard-Telm L, Koeppen H, Lasky LA |
---|
Title | PTEN 2, a Golgi-associated testis-specific homologue of the PTEN tumor suppressor lipid phosphatase. |
---|
[14] |
---|
PubMed ID | 11494141 |
---|
Journal | Oncogene |
---|
Year | 2001 |
---|
Volume | 20 |
---|
Pages | 4457-65 |
---|
Authors | Unoki M, Nakamura Y |
---|
Title | Growth-suppressive effects of BPOZ and EGR2, two genes involved in the PTEN signaling pathway. |
---|
[15] |
---|
PubMed ID | 11275328 |
---|
Journal | Trends Genet |
---|
Year | 2001 |
---|
Volume | 17 |
---|
Pages | 221-8 |
---|
Authors | Laporte J, Blondeau F, Buj-Bello A, Mandel JL |
---|
Title | The myotubularin family: from genetic disease to phosphoinositide metabolism. |
---|
[16] |
---|
PubMed ID | 12176037 |
---|
Journal | Biochem Biophys Res Commun |
---|
Year | 2002 |
---|
Volume | 296 |
---|
Pages | 692-7 |
---|
Authors | Caselli A, Mazzinghi B, Camici G, Manao G, Ramponi G |
---|
Title | Some protein tyrosine phosphatases target in part to lipid rafts and interact with caveolin-1. |
---|
[17] |
---|
PubMed ID | 11858936 |
---|
Journal | Cell Signal |
---|
Year | 2002 |
---|
Volume | 14 |
---|
Pages | 285-95 |
---|
Authors | Leslie NR, Downes CP |
---|
Title | PTEN: The down side of PI 3-kinase signalling. |
---|
[18] |
---|
Comments | PHOSPHORYLATION OF THR-366; SER-370 AND SER-385 |
---|
Medline ID | 22233751 |
---|
PubMed ID | 12297295 |
---|
Journal | FEBS Lett |
---|
Year | 2002 |
---|
Volume | 528 |
---|
Pages | 145-53 |
---|
Authors | Miller SJ, Lou DY, Seldin DC, Lane WS, Neel BG |
---|
Title | Direct identification of PTEN phosphorylation sites. |
---|
Related UniProtKB | O00633 |
---|
[19] |
---|
PubMed ID | 12808147 |
---|
Journal | Proc Natl Acad Sci U S A |
---|
Year | 2003 |
---|
Volume | 100 |
---|
Pages | 7491-6 |
---|
Authors | Das S, Dixon JE, Cho W |
---|
Title | Membrane-binding and activation mechanism of PTEN. |
---|
comments | The C-terminal of this enzyme, which seems to be phosphorylated, is not included in the PDB structure. According to the literature [4], Cys124, Arg130 and Asp92 are catalytic residues. Asp92 acts as a general acid, to protonate the leaving, phonolic oxgen atom. As the catalytic domain of this enzyme is homologous to dual specificity protein phosphatase 3 (Swiss-prot;P51452; S00458 in EzCatDB), the catalytic mechanism can be similar to that. In the protein phosphatase 3, Ser131 facilitates the reaction by interacting with the thiol group of Cys124. The corresponding residue, Thr131 also seems to interact with Cys124 in this enzyme structure (PDB; 1d5r).
|
created | updated |
---|
2004-08-17 | 2009-02-26 |
|
|