EzCatDB: D00170
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DB codeD00170
CATH domainDomain 11.10.530.10 : LysozymeCatalytic domain
Domain 23.30.20.10 : Endochitinase; domain 2Catalytic domain
E.C.3.2.1.14
CSA1cns

CATH domainRelated DB codes (homologues)
1.10.530.10 : LysozymeS00520,S00509

Enzyme Name
UniProtKBKEGG

P23951O81934
Protein name26 kDa endochitinase 2
chitinase
chitodextrinase
1,4-beta-poly-N-acetylglucosaminidase
poly-beta-glucosaminidase
beta-1,4-poly-N-acetyl glucosamidinase
poly[1,4-(N-acetyl-beta-D-glucosaminide)] glycanohydrolase
SynonymsEC 3.2.1.14
CHI-26
Chitinase
PfamPF00182 (Glyco_hydro_19)
[Graphical view]
PF00182 (Glyco_hydro_19)
[Graphical view]
CAZyGH19 (Glycoside Hydrolase Family)
GH19 (Glycoside Hydrolase Family)

KEGG pathways
MAP codePathways
MAP00530Aminosugars metabolism

UniProtKB:Accession NumberP23951O81934
Entry nameCHI2_HORVUO81934_CANEN
ActivityRandom hydrolysis of N-acetyl-beta-D- glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subunit

Subcellular location

Cofactor


Compound table: links to PDB-related databases & PoSSuM

SubstratesProducts
KEGG-idC00461C00851C00001C03518C00140
CompoundChitinChitodextrinH2ON-Acetyl-D-glucosaminideN-Acetyl-D-glucosamine
Typeamide group,polysaccharideamide group,polysaccharideH2Oamide group,carbohydrateamide group,carbohydrate
ChEBI

15377

506227
PubChem

962
22247451

439174
             
1baaA01UnboundUnbound UnboundUnbound
1cnsA01UnboundUnbound UnboundUnbound
1cnsB01UnboundUnbound UnboundUnbound
2baaA01UnboundUnbound UnboundUnbound
1dxjA01UnboundUnbound UnboundUnbound
1baaA02UnboundUnbound UnboundUnbound
1cnsA02UnboundUnbound UnboundUnbound
1cnsB02UnboundUnbound UnboundUnbound
2baaA02UnboundUnbound UnboundUnbound
1dxjA02UnboundUnbound UnboundUnbound

Active-site residues
pdbCatalytic residues
         
1baaA01GLU 67
1cnsA01GLU 67
1cnsB01GLU 67
2baaA01GLU 67
1dxjA01GLU 68
1baaA02GLU 89
1cnsA02GLU 89
1cnsB02GLU 89
2baaA02GLU 89
1dxjA02GLU 90

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[3]p.410-411

references
[1]
PubMed ID1593638
JournalJ Mol Biol
Year1992
Volume225
Pages565-7
AuthorsHart PJ, Ready MP, Robertus JD
TitleCrystallization of an endochitinase from Hordeum vulgar L. seeds.
[2]
CommentsX-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS)
Medline ID93132784
PubMed ID8421299
JournalJ Mol Biol
Year1993
Volume229
Pages189-93
AuthorsHart PJ, Monzingo AF, Ready MP, Ernst SR, Robertus JD
TitleCrystal structure of an endochitinase from Hordeum vulgare L. seeds.
Related UniProtKBP23951
[3]
CommentsX-ray crystallography
PubMed ID7739049
JournalJ Mol Biol
Year1995
Volume248
Pages402-13
AuthorsHart PJ, Pfluger HD, Monzingo AF, Hollis T, Robertus JD
TitleThe refined crystal structure of an endochitinase from Hordeum vulgare L. seeds at 1.8 A resolution.
Related PDB1baa,2baa
[4]
CommentsX-ray crystallography
JournalActa Crystallogr D Biol Crystallogr
Year1996
Volume52
Pages289-98
AuthorsSong HK, Suh SW
TitleRefined structure of the chitinase from barley seeds at 2.0 angstrom resolution.
Related PDB1cns
[5]
PubMed ID8831791
JournalJ Mol Biol
Year1996
Volume262
Pages243-57
AuthorsTerwisscha van Scheltinga AC, Hennig M, Dijkstra BW
TitleThe 1.8 A resolution structure of hevamine, a plant chitinase/lysozyme, and analysis of the conserved sequence and structure motifs of glycosyl hydrolase family 18.
[6]
PubMed ID9148754
JournalBiochem J
Year1997
Volume322
Pages815-22
AuthorsAndersen MD, Jensen A, Robertus JD, Leah R, Skriver K
TitleHeterologous expression and characterization of wild-type and mutant forms of a 26 kDa endochitinase from barley (Hordeum vulgare L.).
[7]
PubMed ID9790582
JournalMol Gen Genet
Year1998
Volume259
Pages511-5
AuthorsOhme-Takagi M, Meins F Jr, Shinshi H
TitleA tobacco gene encoding a novel basic class II chitinase: a putative ancestor of basic class I and acidic class II chitinase genes.
[8]
CommentsX-ray crystallography
JournalActa Crystallogr D Biol Crystallogr
Year2000
Volume56
Pages1096-9
AuthorsHahn M, Hennig M, Schlesier B, Hohne W
TitleStructure of jack bean chitinase.
Related PDB1dxj
[9]
PubMed ID11414619
JournalPlant Mol Biol
Year2001
Volume45
Pages609-18
AuthorsSuarez V, Staehelin C, Arango R, Holtorf H, Hofsteenge J, Meins F Jr
TitleSubstrate specificity and antifungal activity of recombinant tobacco class I chitinases.


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2004-03-252009-09-29


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