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CATH domain | Related DB codes (homologues) |
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3.40.50.1820 : Rossmann fold | S00544,S00344,S00517,S00525,S00526,S00720,S00723,S00724,S00725,S00919,S00057,S00374,S00345,S00347,S00348,S00346,S00350,S00352,S00353,S00355,S00356,S00358,D00210,D00539,T00253 |
Enzyme Name | UniProtKB | KEGG |
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| P00729 |
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Protein name | Carboxypeptidase Y | carboxypeptidase Ccarboxypeptidase Yserine carboxypeptidase Icathepsin Alysosomal protective proteindeamidaselysosomal carboxypeptidase Aphaseolin |
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Synonyms | EC 3.4.16.5Carboxypeptidase YSCY |
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RefSeq | NP_014026.1 (Protein) NM_001182806.1 (DNA/RNA sequence)
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MEROPS | S10.001 (Serine)
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Pfam | PF05388 (Carbpep_Y_N) PF00450 (Peptidase_S10) [Graphical view]
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UniProtKB:Accession Number | P00729 |
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Entry name | CBPY_YEAST |
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Activity | Release of a C-terminal amino acid with broad specificity. |
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Subunit |
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Subcellular location | Vacuole. Note=Lysosome-like vacuoles. |
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Cofactor |
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References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
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[2] | p.11116 |
| [4] | Fig.8 |
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references | [1] |
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PubMed ID | 1459131 |
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Journal | Eur J Biochem |
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Year | 1992 |
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Volume | 210 |
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Pages | 467-73 |
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Authors | Christensen U, Drohse HB, Molgaard L |
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Title | Mechanism of carboxypeptidase-Y-catalysed peptide semisynthesis. |
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[2] |
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Comments | X-ray crystallography (2.8 Angstroms) |
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PubMed ID | 7727362 |
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Journal | Biochemistry |
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Year | 1994 |
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Volume | 33 |
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Pages | 11106-20 |
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Authors | Endrizzi JA, Breddam K, Remington SJ |
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Title | 2.8-A structure of yeast serine carboxypeptidase. |
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Related PDB | 1cpy,1ysc |
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Related UniProtKB | P00729 |
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[3] |
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Comments | catalysis |
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Journal | J Am Chem Soc |
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Year | 1995 |
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Volume | 117 |
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Pages | 5944 |
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Authors | Sorensen SB, Raaschou-Nielsen M, Mortensen UH, Remington SJ, Breddam K |
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Title | Site-directed mutagenesis on (serine) carboxypeptidase y from yeast. The significance of thr 60 and met 398 in hydrolysis and aminolysis reactions. |
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Related PDB | 1cpy |
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[4] |
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PubMed ID | 8679540 |
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Journal | Biochemistry |
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Year | 1996 |
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Volume | 35 |
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Pages | 7131-41 |
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Authors | Stennicke HR, Mortensen UH, Breddam K |
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Title | Studies on the hydrolytic properties of (serine) carboxypeptidase Y. |
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[5] |
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Comments | catalysis (ligand specificity) |
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PubMed ID | 9336845 |
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Journal | Protein Sci |
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Year | 1997 |
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Volume | 6 |
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Pages | 2227-32 |
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Authors | Sorensen SB, Breddam K |
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Title | The specificity of carboxypeptidase Y may be altered by changing the hydrophobicity of the S'1 binding pocket. |
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[6] |
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PubMed ID | 10021925 |
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Journal | Bioorg Med Chem Lett |
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Year | 1999 |
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Volume | 9 |
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Pages | 187-92 |
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Authors | Mock WL, Xu D |
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Title | Catalytic activity of carboxypeptidase B and of carboxypeptidase Y with anisylazoformyl substrates. |
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[7] |
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Comments | conformational stability |
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PubMed ID | 10336632 |
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Journal | Eur J Biochem |
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Year | 1999 |
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Volume | 262 |
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Pages | 475-83 |
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Authors | Dumoulin M, Ueno H, Hayashi R, Balny C |
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Title | Contribution of the carbohydrate moiety to conformational stability of the carboxypeptidase Y high pressure study. |
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[8] |
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Comments | catalysis (mutation analysis) |
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PubMed ID | 10427689 |
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Journal | Biosci Biotechnol Biochem |
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Year | 1999 |
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Volume | 63 |
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Pages | 1045-50 |
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Authors | Shimizu H, Ueno H, Hayashi R |
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Title | Role of carbohydrate moiety in carboxypeptidase Y: structural study of mutant enzyme lacking carbohydrate moiety. |
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comments | This enzyme belongs to the peptidase family S10. According to the literature [2], this enzyme contains a catalytic triad, Ser146/Asp338/His397 (PDB; 1cpy), which is similar to those of trypsine-like serine peptidases. In this enzyme, Ser146 acts as a nucleophile, and His397 acts as Acid/Base. The negatively charged tetrahedral intermediate during catalysis is stablized by the oxyanion hole composed of the backbone amides.
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created | updated |
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2003-01-27 | 2011-02-16 |
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