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CATH domain | Related DB codes (homologues) |
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2.40.10.10 : Thrombin, subunit H | M00139,D00214,M00167,D00426,M00133,D00428,D00429,D00430,D00431,D00432,D00433,D00434,D00435,M00227,M00209,D00194,D00197,D00212,D00216,M00212,D00224,D00497,M00217,M00216,D00528,D00848,D00850,D00851,D00852,D00855,M00152,M00155,M00157,M00181,M00315,M00316,M00317,M00348,M00349,T00074,T00410,T00411 |
Enzyme Name | UniProtKB | KEGG |
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| P00771 |
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Protein name | Brachyurin | brachyurinUca pugilator collagenolytic proteinasecrab protease Icrab protease II |
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Synonyms | EC 3.4.21.32Collagenolytic protease |
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MEROPS | S01.122 (Serine)
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Pfam | PF00089 (Trypsin) [Graphical view]
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UniProtKB:Accession Number | P00771 |
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Entry name | COGS_UCAPU |
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Activity | Hydrolysis of proteins, with broad specificity for peptide bonds. Native collagen is cleaved about 75% of the length of the molecule from the N-terminus. Low activity on small molecule substrates of both trypsin and chymotrypsin. |
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Subunit |
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Subcellular location |
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Cofactor |
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Compound table: links to PDB-related databases & PoSSuM |
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| Substrates | Products | intermediates |
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KEGG-id | C00017 | C00012 | C00211 | C00001 | C00017 | C00012 | I00087 | I00085 | I00086 |
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Compound | Protein | Peptide | Collagen | H2O | Protein | Peptide | Peptidyl-tetrahedral intermediate | Acyl-enzyme | Tetrahedral intermediate |
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Type | peptide/protein | peptide/protein | amine group,carboxyl group,peptide/protein | H2O | peptide/protein | peptide/protein |
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ChEBI |
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| 15377
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PubChem |
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| 22247451 962
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1azzA01 |  |  |  |  |  |  |  | Analogue:MET 84-MET 85(chain C) | Unbound | Unbound | | Unbound | Unbound | | | |
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1azzB01 |  |  |  |  |  |  |  | Analogue:MET 84-MET 85(chain D) | Unbound | Unbound | | Unbound | Unbound | | | |
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1azzA02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | | | |
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1azzB02 |  |  |  |  |  |  |  | Unbound | Unbound | Unbound | | Unbound | Unbound | | | |
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References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
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[14] | Fig.1 |
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references | [1] |
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PubMed ID | 6781531 |
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Journal | Biochemistry |
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Year | 1980 |
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Volume | 19 |
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Pages | 6089-95 |
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Authors | Grant GA, Eisen AZ |
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Title | Substrate specificity of the collagenolytic serine protease from Uca pugilator: studies with noncollagenous substrates. |
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[2] |
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PubMed ID | 6756469 |
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Journal | Biochemistry |
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Year | 1982 |
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Volume | 21 |
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Pages | 5183-9 |
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Authors | Welgus HG, Grant GA, Jeffrey JJ, Eisen AZ |
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Title | Substrate specificity of the collagenolytic serine protease from Uca pugilator: studies with collagenous substrates. |
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[3] |
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PubMed ID | 6337626 |
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Journal | Biochemistry |
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Year | 1983 |
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Volume | 22 |
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Pages | 354-8 |
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Authors | Grant GA, Sacchettini JC, Welgus HG |
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Title | A collagenolytic serine protease with trypsin-like specificity from the fiddler crab Uca pugilator. |
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[4] |
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PubMed ID | 6305411 |
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Journal | Biochemistry |
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Year | 1983 |
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Volume | 22 |
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Pages | 2228-33 |
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Authors | Welgus HG, Grant GA |
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Title | Degradation of collagen substrates by a trypsin-like serine protease from the fiddler crab Uca pugilator. |
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[5] |
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Medline ID | 94208517 |
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PubMed ID | 8156987 |
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Journal | EMBO J |
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Year | 1994 |
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Volume | 13 |
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Pages | 1502-7 |
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Authors | McGrath ME, Erpel T, Bystroff C, Fletterick RJ |
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Title | Macromolecular chelation as an improved mechanism of protease inhibition: structure of the ecotin-trypsin complex. |
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[6] |
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PubMed ID | 8034725 |
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Journal | J Biol Chem |
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Year | 1994 |
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Volume | 269 |
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Pages | 19565-72 |
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Authors | Tsu CA, Perona JJ, Schellenberger V, Turck CW, Craik CS |
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Title | The substrate specificity of Uca pugilator collagenolytic serine protease 1 correlates with the bovine type I collagen cleavage sites. |
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[7] |
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Medline ID | 95276656 |
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PubMed ID | 7757004 |
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Journal | Protein Sci |
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Year | 1995 |
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Volume | 4 |
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Pages | 141-8 |
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Authors | McGrath ME, Gillmor SA, Fletterick RJ |
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Title | Ecotin: lessons on survival in a protease-filled world. |
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[8] |
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PubMed ID | 7795518 |
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Journal | Protein Sci |
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Year | 1995 |
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Volume | 4 |
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Pages | 337-60 |
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Authors | Perona JJ, Craik CS |
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Title | Structural basis of substrate specificity in the serine proteases. |
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[9] |
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PubMed ID | 7788296 |
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Journal | Structure |
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Year | 1995 |
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Volume | 3 |
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Pages | 309-16 |
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Authors | Schiltz M, Fourme R, Broutin I, Prange T |
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Title | The catalytic site of serine proteinases as a specific binding cavity for xenon. |
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[10] |
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PubMed ID | 8626718 |
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Journal | J Biol Chem |
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Year | 1996 |
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Volume | 271 |
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Pages | 11563-70 |
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Authors | Tsu CA, Craik CS |
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Title | Substrate recognition by recombinant serine collagenase 1 from Uca pugilator. |
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[11] |
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Medline ID | 97084808 |
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PubMed ID | 8931142 |
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Journal | Protein Sci |
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Year | 1996 |
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Volume | 5 |
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Pages | 2236-47 |
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Authors | Shin DH, Song HK, Seong IS, Lee CS, Chung CH, Suh SW |
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Title | Crystal structure analyses of uncomplexed ecotin in two crystal forms: implications for its function and stability. |
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[12] |
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Comments | X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), AND REVISIONS |
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Medline ID | 97299771 |
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PubMed ID | 9154920 |
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Journal | Biochemistry |
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Year | 1997 |
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Volume | 36 |
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Pages | 5381-92 |
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Authors | Perona JJ, Tsu CA, Craik CS, Fletterick RJ |
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Title | Crystal structure of an ecotin-collagenase complex suggests a model for recognition and cleavage of the collagen triple helix. |
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Related PDB | 1azz |
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Related UniProtKB | P00771 |
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[13] |
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PubMed ID | 9154921 |
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Journal | Biochemistry |
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Year | 1997 |
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Volume | 36 |
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Pages | 5393-401 |
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Authors | Tsu CA, Perona JJ, Fletterick RJ, Craik CS |
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Title | Structural basis for the broad substrate specificity of fiddler crab collagenolytic serine protease 1. |
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[14] |
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PubMed ID | 9374470 |
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Journal | J Biol Chem |
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Year | 1997 |
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Volume | 272 |
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Pages | 29987-90 |
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Authors | Perona JJ, Craik CS |
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Title | Evolutionary divergence of substrate specificity within the chymotrypsin-like serine protease fold. |
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[15] |
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PubMed ID | 11121112 |
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Journal | Eur J Biochem |
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Year | 2001 |
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Volume | 268 |
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Pages | 127-31 |
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Authors | Benjamin DC, Kristjansdottir S, Gudmundsdottir A |
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Title | Increasing the thermal stability of euphauserase. A cold-active and multifunctional serine protease from Antarctic krill. |
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[16] |
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PubMed ID | 12437096 |
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Journal | Biol Chem |
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Year | 2002 |
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Volume | 383 |
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Pages | 1125-31 |
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Authors | Gudmundsdottir A |
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Title | Cold-adapted and mesophilic brachyurins. |
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[17] |
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PubMed ID | 14731305 |
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Journal | BMC Struct Biol |
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Year | 2004 |
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Volume | 4 |
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Pages | 2 |
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Authors | Rudenskaya GN, Kislitsin YA, Rebrikov DV |
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Title | Collagenolytic serine protease PC and trypsin PC from king crab Paralithodes camtschaticus: cDNA cloning and primary structure of the enzymes. |
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[18] |
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PubMed ID | 15014068 |
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Journal | J Biol Chem |
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Year | 2004 |
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Volume | 279 |
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Pages | 21500-10 |
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Authors | Wlodawer A, Li M, Gustchina A, Tsuruoka N, Ashida M, Minakata H, Oyama H, Oda K, Nishino T, Nakayama T |
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Title | Crystallographic and biochemical investigations of kumamolisin-As, a serine-carboxyl peptidase with collagenase activity. |
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comments | This enzyme belongs to the peptidase family-S1. As this enzyme has got a catalytic triad composed of Ser/His/Asp, it should have a similar mechanism to that of trypsin (D00197 in EzCatDB).
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created | updated |
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2004-04-27 | 2011-02-18 |
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