EzCatDB: D00214
Related links:    PDB-formatted query search system Fasta-formatted query search system Fasta-formatted query search system

DB codeD00214
RLCP classification1.13.30000.10 : Hydrolysis
CATH domainDomain 12.40.10.10 : Thrombin, subunit HCatalytic domain
Domain 22.40.10.10 : Thrombin, subunit HCatalytic domain
E.C.3.4.21.36

CATH domainRelated DB codes (homologues)
2.40.10.10 : Thrombin, subunit HM00139,M00167,D00426,M00133,D00428,D00429,D00430,D00431,D00432,D00433,D00434,D00435,M00227,M00209,D00194,D00197,D00211,D00212,D00216,M00212,D00224,D00497,M00217,M00216,D00528,D00848,D00850,D00851,D00852,D00855,M00152,M00155,M00157,M00181,M00315,M00316,M00317,M00348,M00349,T00074,T00410,T00411

Enzyme Name
UniProtKBKEGG

Q7SIG3P00772
Protein nameElastase-1Elastase-1pancreatic elastase
pancreatopeptidase E
pancreatic elastase I
elastase
elaszym
serine elastase
SynonymsEC 3.4.21.36
EC 3.4.21.36
RefSeq
NP_998988.1 (Protein)
NM_213823.1 (DNA/RNA sequence)
MEROPSS01.153 (Serine)
S01.153 (Serine)
PfamPF00089 (Trypsin)
[Graphical view]
PF00089 (Trypsin)
[Graphical view]


UniProtKB:Accession NumberQ7SIG3P00772
Entry nameELA1_SALSAELA1_PIG
ActivityHydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.
Subunit

Subcellular locationSecreted.Secreted.
CofactorBinds 1 calcium ion per subunit.Binds 1 calcium ion per subunit.

Compound table: links to PDB-related databases & PoSSuM

CofactorsSubstratesProductsintermediates
KEGG-idC00076C00017C00012C00373C00001C00098C00017C00012I00087I00085I00086
CompoundCalciumProteinPeptideElastinH2OOligopeptideProteinPeptidePeptidyl-tetrahedral intermediateAcyl-enzymeTetrahedral intermediate
Typedivalent metal (Ca2+, Mg2+)peptide/proteinpeptide/proteinpeptide/proteinH2Oamine group,carboxyl group,peptide/proteinpeptide/proteinpeptide/protein


ChEBI29108



15377






PubChem271


439221
962
22247451






                   
1eltA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1b0eA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundTransition-state-analogue:SEIUnboundUnbound
1bmaA01UnboundUnboundAnalogue:TFA-LEU-MBH-ISO(chain B)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
1btuA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:2BLUnbound
1c1mA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1e34B01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:TPXUnbound
1e35B01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:TPXUnbound
1e36B01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:TPYUnbound
1e37B01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:TPYUnbound
1e38B01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:TPYUnbound
1eaiA01UnboundBound:LEU 31-MET 32(chain C)UnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1eaiB01UnboundBound:LEU 31-MET 32(chain D)UnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1easA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundTransition-state-analogue:TFKUnboundUnbound
1eatA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundTransition-state-analogue:TFIUnboundUnbound
1eauA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundTransition-state-analogue:BDKUnboundUnbound
1elaA01UnboundUnboundAnalogue:TFA-LYS-PRO-ISO(chain B)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
1elbA01UnboundUnboundAnalogue:TFA-LYS-LEU-ISO(chain B)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
1elcA01UnboundUnboundAnalogue:TFA-LYS-PHE-ISO(chain B)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
1eldE01UnboundUnboundAnalogue:TFA-PHE-ALA-ANI(chain I)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
1eleE01UnboundUnboundAnalogue:TFA-VAL-ALA-ANI(chain I)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
1elfA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:BAFUnbound
1elgA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:BAAUnbound
1esaA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1esbA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1estA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundTransition-state-analogue:TOS
1fleE01UnboundBound:ALA 24-MET 25(chain I)UnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1fzzA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundTransition-state-analogue:681UnboundUnbound
1gvkB01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:ACE-ASN-PRO-ILE(chain A)Unbound
1gwaA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1h9lB01UnboundUnboundAnalogue:VAL-GLU-PRO-ILE(chain A)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
1haxB01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-bound:VAL-GLU-PRO-ILE(chain A)Unbound
1hayB01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1hazB01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundTransition-state-bound:VAL-GLU-PRO-ILE(chain A)
1hb0B01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1hv7A01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:616Unbound
1incA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:ICLUnbound
1jimA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:ICUUnbound
1l0zA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1l1gA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1lkaA01UnboundUnboundUnboundUnbound Analogue:ACTUnboundUnboundUnboundUnboundUnbound
1lkbA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1lvyA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1mcvA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1mmjN01UnboundUnboundUnboundUnbound UnboundUnboundUnboundTransition-state-analogue:FR1UnboundUnbound
1nesE01UnboundUnboundUnboundUnbound Bound:ACE-ALA-PRO-ALA(chain J)UnboundAnalogue:ACE-ALA-PRO-ALA(chain I)UnboundUnboundUnbound
1okxA01UnboundUnboundAnalogue:1BO-ALA-THR-THR-LEU-SUJ-CNT-VAL(chain C)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
1okxB01UnboundUnboundAnalogue:1BO-ALA-THR-THR-LEU-SUJ-CNT-VAL(chain D)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
1qgfA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:TPXUnbound
1qixB01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-bound:VAL-GLU-PRO-ILE(chain A)Unbound
1qnjA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1qr3E01UnboundAnalogue:AIB-ORN-THR-ABA-AA4-PHE-AA6-VAL(chain I)UnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1uo6A01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1uvoA01UnboundUnboundUnboundUnbound Analogue:ACTUnboundUnboundUnboundUnboundUnbound
1uvpA01UnboundUnboundUnboundUnbound Analogue:ACTUnboundUnboundUnboundUnboundUnbound
2estE01UnboundUnboundAnalogue:TFA-LYS-ALA-ANI(chain I)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
3estA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
4estE01UnboundUnboundUnboundUnbound UnboundUnboundUnboundTransition-state-analogue:ACE-ALA-PRO-VAL-FPA(chain I)UnboundUnbound
5estE01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:PHQ-ALA-B2I(chain I)Unbound
6estA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
7estE01UnboundUnboundAnalogue:TFA-LEU-ALA-ANI(chain I)Unbound UnboundUnboundUnboundUnboundUnboundUnbound
8estE01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:GISUnbound
9estA01UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundIntermediate-analogue:IBRUnbound
1eltA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1b0eA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1bmaA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1btuA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1c1mA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1e34B02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1e35B02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1e36B02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1e37B02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1e38B02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1eaiA02UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1eaiB02UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1easA02Analogue:_NAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1eatA02Analogue:_NAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1eauA02Analogue:_NAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1elaA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1elbA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1elcA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1eldE02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1eleE02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1elfA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1elgA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1esaA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1esbA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1estA02UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1fleE02UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1fzzA02UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1gvkB02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1gwaA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1h9lB02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1haxB02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1hayB02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1hazB02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1hb0B02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1hv7A02UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1incA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1jimA02UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1l0zA02Analogue:_NAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1l1gA02Analogue:_NAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1lkaA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1lkbA02Analogue:_NAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1lvyA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1mcvA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1mmjN02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1nesE02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1okxA02UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1okxB02UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1qgfA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1qixB02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1qnjA02Analogue:_NAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1qr3E02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1uo6A02Analogue:_NAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1uvoA02Analogue:_CDUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
1uvpA02Analogue:_CDUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
2estE02UnboundUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
3estA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
4estE02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
5estE02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
6estA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
7estE02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
8estE02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound
9estA02Bound:_CAUnboundUnboundUnbound UnboundUnboundUnboundUnboundUnboundUnbound

Active-site residues
resource
Swiss-prot;P00772 & PDB;1bma
pdbCatalytic residuesCofactor-binding residuesMain-chain involved in catalysis
           
1eltA01SER 195
 
GLY 193;SER 195
1b0eA01SER 195
 
GLY 193;SER 195
1bmaA01SER 203
 
GLY 201;SER 203
1btuA01SER 195
 
GLY 193;SER 195
1c1mA01SER 195
 
GLY 193;SER 195
1e34B01SER 195
 
GLY 193;SER 195
1e35B01SER 195
 
GLY 193;SER 195
1e36B01SER 195
 
GLY 193;SER 195
1e37B01SER 195
 
GLY 193;SER 195
1e38B01SER 195
 
GLY 193;SER 195
1eaiA01SER 195
 
GLY 193;SER 195
1eaiB01SER 195
 
GLY 193;SER 195
1easA01SER 195
 
GLY 193;SER 195
1eatA01SER 195
 
GLY 193;SER 195
1eauA01SER 195
 
GLY 193;SER 195
1elaA01SER 203
 
GLY 201;SER 203
1elbA01SER 203
 
GLY 201;SER 203
1elcA01SER 203
 
GLY 201;SER 203
1eldE01SER 203
 
GLY 201;SER 203
1eleE01SER 203
 
GLY 201;SER 203
1elfA01SER 203
 
GLY 201;SER 203
1elgA01SER 203
 
GLY 201;SER 203
1esaA01SER 203
 
GLY 201;SER 203
1esbA01SER 203
 
GLY 201;SER 203
1estA01SER 195
 
GLY 193;SER 195
1fleE01SER 195
 
GLY 193;SER 195
1fzzA01SER 195
 
GLY 193;SER 195
1gvkB01SER 195
 
GLY 193;SER 195
1gwaA01SER 195
 
GLY 193;SER 195
1h9lB01SER 195
 
GLY 193;SER 195
1haxB01SER 195
 
GLY 193;SER 195
1hayB01SER 195
 
GLY 193;SER 195
1hazB01SER 195
 
GLY 193;SER 195
1hb0B01SER 195
 
GLY 193;SER 195
1hv7A01SER 203
 
GLY 201;SER 203
1incA01SER 195
 
GLY 193;SER 195
1jimA01SER 195
 
GLY 193;SER 195
1l0zA01SER 188
 
GLY 186;SER 188
1l1gA01SER 188
 
GLY 186;SER 188
1lkaA01SER 188
 
GLY 186;SER 188
1lkbA01SER 188
 
GLY 186;SER 188
1lvyA01SER 195
 
GLY 193;SER 195
1mcvA01SER 195
 
GLY 193;SER 195
1mmjN01SER 188
 
GLY 186;SER 188
1nesE01SER 195
 
GLY 193;SER 195
1okxA01SER 203
 
GLY 201;SER 203
1okxB01SER 203
 
GLY 201;SER 203
1qgfA01SER 195
 
GLY 193;SER 195
1qixB01SER 195
 
GLY 193;SER 195
1qnjA01SER 195
 
GLY 193;SER 195
1qr3E01SER 195
 
GLY 193;SER 195
1uo6A01SER 188
 
GLY 186;SER 188
1uvoA01SER 188
 
GLY 186;SER 188
1uvpA01SER 188
 
GLY 186;SER 188
2estE01SER 195
 
GLY 193;SER 195
3estA01SER 195
 
GLY 193;SER 195
4estE01SER 195
 
GLY 193;SER 195
5estE01SER 195
 
GLY 193;SER 195
6estA01SER 195
 
GLY 193;SER 195
7estE01SER 195
 
GLY 193;SER 195
8estE01SER 195
 
GLY 193;SER 195
9estA01SER 195
 
GLY 193;SER 195
1eltA02HIS 57;ASP 102
GLU 70;ASN 72;THR 75;GLU 77;GLU 80(Calcium binding)
 
1b0eA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1bmaA02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1btuA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1c1mA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASP 77;GLU 80(Calcium binding)
 
1e34B02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1e35B02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1e36B02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1e37B02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1e38B02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1eaiA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASP 77;GLU 80(Calcium binding)
 
1eaiB02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASP 77;GLU 80(Calcium binding)
 
1easA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1eatA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1eauA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1elaA02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1elbA02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1elcA02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1eldE02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1eleE02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1elfA02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1elgA02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1esaA02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1esbA02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1estA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1fleE02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1fzzA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASP 77;GLU 80(Calcium binding)
 
1gvkB02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1gwaA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASP 77;GLU 80(Calcium binding)
 
1h9lB02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1haxB02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1hayB02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1hazB02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1hb0B02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1hv7A02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASP 81;GLU 84(Calcium binding)
 
1incA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1jimA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1l0zA02HIS 45;ASP  93
GLU 59;ASN 61;GLN 64;ASP 66;GLU 69(Calcium binding)
 
1l1gA02HIS 45;ASP  93
GLU 59;ASN 61;GLN 64;ASP 66;GLU 69(Calcium binding)
 
1lkaA02HIS 45;ASP  93
GLU 59;ASN 61;GLN 64;ASP 66;GLU 69(Calcium binding)
 
1lkbA02HIS 45;ASP  93
GLU 59;ASN 61;GLN 64;ASP 66;GLU 69(Calcium binding)
 
1lvyA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1mcvA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASP 77;GLU 80(Calcium binding)
 
1mmjN02HIS 45;ASP  93
GLU 59;ASN 61;GLN 64;ASP 66;GLU 69(Calcium binding)
 
1nesE02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1okxA02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1okxB02HIS 60;ASP 108
GLU 74;ASN 76;GLN 79;ASN 81;GLU 84(Calcium binding)
 
1qgfA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1qixB02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1qnjA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASP 77;GLU 80(Calcium binding)
 
1qr3E02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
1uo6A02HIS 45;ASP  93
GLU 59;ASN 61;GLN 64;ASP 66;GLU 69(Calcium binding)
 
1uvoA02HIS 45;ASP  93
GLU 59;ASN 61;GLN 64;ASP 66;GLU 69(Calcium binding)
 
1uvpA02HIS 45;ASP  93
GLU 59;ASN 61;GLN 64;ASP 66;GLU 69(Calcium binding)
 
2estE02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
3estA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
4estE02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
5estE02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
6estA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
7estE02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
8estE02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 
9estA02HIS 57;ASP 102
GLU 70;ASN 72;GLN 75;ASN 77;GLU 80(Calcium binding)
 

References for Catalytic Mechanism
ReferencesSectionsNo. of steps in catalysis
[7]Fig.2
[10]p.1958
[26]Scheme 1, p.7755
[30]Fig.3
[33]Fig.7
[37]Fig.1
[38]Scheme 2
[42]Scheme 2
[43]Fig.1
[50]Fig.1

references
[1]
CommentsX-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS), AND ACTIVE SITE
PubMed ID5415110
JournalNature
Year1970
Volume225
Pages811-6
AuthorsShotton DM, Watson HC
TitleThree-dimensional structure of tosyl-elastase.
Related UniProtKBP00772
[2]
CommentsX-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)
PubMed ID628010
JournalJ Mol Biol
Year1978
Volume118
Pages137-208
AuthorsSawyer L, Shotton DM, Campbell JW, Wendell PL, Muirhead H, Watson HC
TitleThe atomic structure of crystalline porcine pancreatic elastase at 2.5 A resolution: comparisons with the structure of alpha-chymotrypsin.
Related PDB1est
Related UniProtKBP00772
[3]
CommentsX-ray crystallography
PubMed ID6926029
JournalJ Mol Biol
Year1982
Volume162
Pages645-58
AuthorsHughes DL, Sieker LC, Bieth J, Dimicoli JL
TitleCrystallographic study of the binding of a trifluoroacetyl dipeptide anilide inhibitor with elastase.
Related PDB2est
[4]
CommentsX-ray crystallography
JournalJ Am Chem Soc
Year1985
Volume107
Pages4091-3
AuthorsMeyer EF Jr, Presta LG, Radhakrishnan R
TitleStereospecific reaction of 3-methoxy-4-chloro-7-aminoisocoumarin with crystalline porcine pancreatic elastase.
Related PDB1jim
[5]
CommentsX-ray crystallography
PubMed ID3640831
JournalJ Mol Biol
Year1986
Volume189
Pages533-9
AuthorsMeyer EF Jr, Radhakrishnan R, Cole GM, Presta LG
TitleStructure of the product complex of acetyl-Ala-Pro-Ala with porcine pancreatic elastase at 1.65 A resolution.
Related PDB1nes
[6]
PubMed ID3504964
JournalJ Comput Aided Mol Des
Year1987
Volume1
Pages211-7
AuthorsLesyng B, Meyer EF Jr
TitleEnergy minimization and molecular dynamics studies of Asn-102 elastase.
[7]
CommentsX-ray crystallography
PubMed ID3430613
JournalJ Mol Biol
Year1987
Volume198
Pages417-24
AuthorsRadhakrishnan R, Presta LG, Meyer EF Jr, Wildonger R
TitleCrystal structures of the complex of porcine pancreatic elastase with two valine-derived benzoxazinone inhibitors.
Related PDB1inc
[8]
CommentsX-ray crystallography
PubMed ID3271103
JournalActa Crystallogr B
Year1988
Volume44
Pages26-38
AuthorsMeyer E, Cole G, Radhakrishnan R, Epp O
TitleStructure of native porcine pancreatic elastase at 1.65 A resolutions.
Related PDB3est
[9]
PubMed ID3349061
JournalBiochemistry
Year1988
Volume27
Pages725-30
AuthorsMeyer EF Jr, Clore GM, Gronenborn AM, Hansen HA
TitleAnalysis of an enzyme-substrate complex by X-ray crystallography and transferred nuclear Overhauser enhancement measurements: porcine pancreatic elastase and a hexapeptide.
[10]
PubMed ID2655701
JournalBiochemistry
Year1989
Volume28
Pages1951-63
AuthorsBode W, Meyer E Jr, Powers JC
TitleHuman leukocyte and porcine pancreatic elastase: X-ray crystal structures, mechanism, substrate specificity, and mechanism-based inhibitors.
[11]
CommentsX-ray crystallography
PubMed ID2611205
JournalBiochemistry
Year1989
Volume28
Pages7610-7
AuthorsTakahashi LH, Radhakrishnan R, Rosenfield RE Jr, Meyer EF Jr
TitleCrystallographic analysis of the inhibition of porcine pancreatic elastase by a peptidyl boronic acid: structure of a reaction intermediate.
Related PDB5est
[12]
PubMed ID2930798
JournalBiochim Biophys Acta
Year1989
Volume995
Pages201-3
AuthorsFitzpatrick PF
TitleIdentification of the rate-limiting step in serine proteinases from the effect of temperature on steady-state kinetics.
[13]
CommentsX-ray crystallography
JournalJ Am Chem Soc
Year1989
Volume111
Pages3368-74
AuthorsTakahashi LH, Radhakrishnan R, Rosenfieldjunior RE, Meyer EF Jr, Trainor DA
TitleCrystal structure of the covalent complex formed by a peptidyl .alpha.,.alpha.-difluoro-.beta.-keto amide with porcine pancreatic elastase at 1.78 .ANG. Resolution
Related PDB4est
[14]
CommentsX-ray crystallography
PubMed ID2337582
JournalBiochemistry
Year1990
Volume29
Pages3108-18
AuthorsPowers JC, Oleksyszyn J, Narasimhan SL, Kam CM
TitleReaction of porcine pancreatic elastase with 7-substituted 3-alkoxy-4-chloroisocoumarins: design of potent inhibitors using the crystal structure of the complex formed with 4-chloro-3-ethoxy-7-guanidinoisocoumarin.
Related PDB8est
[15]
PubMed ID2205243
JournalBiol Chem Hoppe Seyler
Year1990
Volume371 Suppl
Pages59-64
AuthorsMeyer E, Geller M
TitleIrregular elastase complexes: crystallographic and molecular dynamics studies of small inhibitors and water.
[16]
PubMed ID2275978
JournalBiopolymers
Year1990
Volume30
Pages781-96
AuthorsGeller M, Carlson-Golab G, Lesyng B, Swanson SM, Meyer EF Jr
TitleDynamic properties of the first enzymatic reaction steps of porcine pancreatic elastase. How rigid is the active site of the native enzyme? Molecular dynamics simulation.
[17]
CommentsX-ray crystallography
PubMed ID2354062
JournalJ Mol Recognit
Year1990
Volume3
Pages36-44
Authorsde la Sierra IL, Papamichael E, Sakarellos C, Dimicoli JL, Prange T
TitleInteraction of the peptide CF3-Leu-Ala-NH-C6H4-CF3 (TFLA) with porcine pancreatic elastase. X-ray studies at 1.8 A.
Related PDB6est,7est
[18]
CommentsX-ray crystallography
PubMed ID1998677
JournalBiochemistry
Year1991
Volume30
Pages2175-83
AuthorsVijayalakshmi J, Meyer EF Jr, Kam CM, Powers JC
TitleStructural study of porcine pancreatic elastase complexed with 7-amino-3-(2-bromoethoxy)-4-chloroisocoumarin as a nonreactivatable doubly covalent enzyme-inhibitor complex.
Related PDB9est
[19]
PubMed ID8298497
JournalBiochem Mol Biol Int
Year1993
Volume31
Pages685-92
AuthorsBru R, Walde P
TitleCatalytic activity of elastase in reverse micelles.
[20]
CommentsX-ray crystallography
PubMed ID8049229
JournalBiochemistry
Year1994
Volume33
Pages9285-93
AuthorsDing X, Rasmussen BF, Petsko GA, Ringe D
TitleDirect structural observation of an acyl-enzyme intermediate in the hydrolysis of an ester substrate by elastase.
Related PDB1esa,1esb
[21]
PubMed ID8005753
JournalInt J Pept Protein Res
Year1994
Volume43
Pages297-304
AuthorsAmour A, Collet A, Dubar C, Reboud-Ravaux M
TitleSynthesis and protease-catalyzed hydrolysis of a novel hydrazinopeptide.
[22]
CommentsX-ray crystallography
PubMed ID7932559
JournalJ Med Chem
Year1994
Volume37
Pages3313-26
AuthorsBernstein PR, Andisik D, Bradley PK, Bryant CB, Ceccarelli C, Damewood JR Jr, Earley R, Edwards PD, Feeney S, Gomes BC, et al
TitleNonpeptidic inhibitors of human leukocyte elastase. 3. Design, synthesis, X-ray crystallographic analysis, and structure-activity relationships for a series of orally active 3-amino-6-phenylpyridin-2-one trifluoromethyl ketones.
Related PDB1eas
[23]
CommentsX-ray crystallography
PubMed ID7656008
JournalNat Struct Biol
Year1994
Volume1
Pages55-8
AuthorsMattos C, Rasmussen B, Ding X, Petsko GA, Ringe D
TitleAnalogous inhibitors of elastase do not always bind analogously.
Related PDB1ela,1elb,1elc
[24]
CommentsX-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF COMPLEX WITH INHIBITOR
PubMed ID7922044
JournalStructure
Year1994
Volume2
Pages679-89
AuthorsHuang K, Strynadka NC, Bernard VD, Peanasky RJ, James MN
TitleThe molecular structure of the complex of Ascaris chymotrypsin/elastase inhibitor with porcine elastase.
Related PDB1eai
Related UniProtKBP00772
[25]
CommentsX-ray crystallography
PubMed ID15299762
JournalActa Crystallogr D Biol Crystallogr
Year1995
Volume51
Pages925-37
AuthorsBerglund GI, Willassen NP, Hordvik A, Smalas AO
TitleStructure of native pancreatic elastase from North Atlantic salmon at 1.61 A resolution.
Related PDB1elt
[26]
CommentsX-ray crystallography
PubMed ID7779821
JournalBiochemistry
Year1995
Volume34
Pages7749-56
AuthorsDing X, Rasmussen BF, Demuth HU, Ringe D, Steinmetz AC
TitleNature of the inactivation of elastase by N-peptidyl-O-aroyl hydroxylamine as a function of pH.
Related PDB1elf,1elg
[27]
CommentsX-ray crystallography
PubMed ID7880814
JournalBiochemistry
Year1995
Volume34
Pages3193-203
AuthorsMattos C, Giammona DA, Petsko GA, Ringe D
TitleStructural analysis of the active site of porcine pancreatic elastase based on the X-ray crystal structures of complexes with trifluoroacetyl-dipeptide-anilide inhibitors.
Related PDB1eld,1ele
[28]
CommentsX-ray crystallography
PubMed ID7837235
JournalJ Med Chem
Year1995
Volume38
Pages212-5
AuthorsBernstein PR, Gomes BC, Kosmider BJ, Vacek EP, Williams JC
TitleNonpeptidic inhibitors of human leukocyte elastase. 6. Design of a potent, intratracheally active, pyridone-based trifluoromethyl ketone.
Related PDB1eau
[29]
CommentsX-ray crystallography
PubMed ID7837246
JournalJ Med Chem
Year1995
Volume38
Pages98-108
AuthorsVeale CA, Bernstein PR, Bryant C, Ceccarelli C, Damewood JR Jr, Earley R, Feeney SW, Gomes B, Kosmider BJ, Steelman GB, et al
TitleNonpeptidic inhibitors of human leukocyte elastase. 5. Design, synthesis, and X-ray crystallography of a series of orally active 5-aminopyrimidin-6-one-containing trifluoromethyl ketones.
Related PDB1eat
[30]
PubMed ID7837244
JournalJ Med Chem
Year1995
Volume38
Pages86-97
AuthorsVeale CA, Damewood JR Jr, Steelman GB, Bryant C, Gomes B, Williams J
TitleNon-peptidic inhibitors of human leukocyte elastase. 4. Design, synthesis, and in vitro and in vivo activity of a series of beta-carbolinone-containing trifluoromethyl ketones.
[31]
PubMed ID7613461
JournalProtein Sci
Year1995
Volume4
Pages613-21
AuthorsHuntington JA, Patston PA, Gettins PG
TitleS-ovalbumin, an ovalbumin conformer with properties analogous to those of loop-inserted serpins.
[32]
CommentsX-ray crystallography
PubMed ID7604279
JournalScience
Year1995
Volume269
Pages66-9
AuthorsPeisach E, Casebier D, Gallion SL, Furth P, Petsko GA, Hogan JC Jr, Ringe D
TitleInteraction of a peptidomimetic aminimide inhibitor with elastase.
Related PDB1bma
[33]
CommentsX-ray crystallography
PubMed ID8794736
JournalBiochemistry
Year1996
Volume35
Pages11570-6
AuthorsTsunemi M, Matsuura Y, Sakakibara S, Katsube Y
TitleCrystal structure of an elastase-specific inhibitor elafin complexed with porcine pancreatic elastase determined at 1.9 A resolution.
Related PDB1fle
[34]
PubMed ID9630949
JournalNat Biotechnol
Year1996
Volume14
Pages595-9
AuthorsMattos C, Ringe D
TitleLocating and characterizing binding sites on proteins.
[35]
CommentsX-ray crystallography
PubMed ID15299973
JournalActa Crystallogr D Biol Crystallogr
Year1997
Volume53
Pages78-92
AuthorsSchiltz M, Shepard W, Fourme R, Prange T, de la Fortelle E, Bricogne G
TitleHigh-pressure krypton gas and statistical heavy-atom refinement: a successful combination of tools for macromolecular structure determination.
Related PDB1lvy
[36]
PubMed ID9159490
JournalJ Mol Biol
Year1997
Volume268
Pages557-69
AuthorsBaker BM, Murphy KP
TitleDissecting the energetics of a protein-protein interaction: the binding of ovomucoid third domain to elastase.
[37]
CommentsX-ray crystallography
PubMed ID9187653
JournalNat Struct Biol
Year1997
Volume4
Pages456-62
AuthorsWilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ
TitleStructure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase.
Related PDB1qix
[38]
CommentsX-ray crystallography
PubMed ID9860865
JournalBiochemistry
Year1998
Volume37
Pages17506-13
AuthorsWilmouth RC, Westwood NJ, Anderson K, Brownlee W, Claridge TD, Clifton IJ, Pritchard GJ, Aplin RT, Schofield CJ
TitleInhibition of elastase by N-sulfonylaryl beta-lactams: anatomy of a stable acyl-enzyme complex.
Related PDB1btu
[39]
PubMed ID9504720
JournalBiol Chem
Year1998
Volume379
Pages71-4
AuthorsHaensler M, Wehofsky N, Gerisch S, Wissmann JD, Jakubke HD
TitleReverse catalysis of elastase from porcine pancreas in frozen aqueous systems.
[40]
CommentsX-ray crystallography
PubMed ID9651152
JournalJ Med Chem
Year1998
Volume41
Pages2461-80
AuthorsCregge RJ, Durham SL, Farr RA, Gallion SL, Hare CM, Hoffman RV, Janusz MJ, Kim HO, Koehl JR, Mehdi S, Metz WA, Peet NP, Pelton JT, Schreuder HA, Sunder S, Tardif C
TitleInhibition of human neutrophil elastase. 4. Design, synthesis, X-ray crystallographic analysis, and structure-activity relationships for a series of P2-modified, orally active peptidyl pentafluoroethyl ketones.
Related PDB1b0e
[41]
CommentsX-ray crystallography
PubMed ID9443341
JournalProteins
Year1998
Volume30
Pages61-73
AuthorsPrange T, Schiltz M, Pernot L, Colloc'h N, Longhi S, Bourguet W, Fourme R
TitleExploring hydrophobic sites in proteins with xenon or krypton.
Related PDB1c1m
[42]
CommentsX-ray crystallography
PubMed ID10387042
JournalBiochemistry
Year1999
Volume38
Pages7989-98
AuthorsWilmouth RC, Kassamally S, Westwood NJ, Sheppard RJ, Claridge TD, Aplin RT, Wright PA, Pritchard GJ, Schofield CJ
TitleMechanistic insights into the inhibition of serine proteases by monocyclic lactams.
Related PDB1qgf
[43]
PubMed ID10455164
JournalJ Biol Chem
Year1999
Volume274
Pages24901-5
AuthorsTaylor P, Anderson V, Dowden J, Flitsch SL, Turner NJ, Loughran K, Walkinshaw MD
TitleNovel mechanism of inhibition of elastase by beta-lactams is defined by two inhibitor crystal complexes.
[44]
CommentsX-ray crystallography
PubMed ID10739939
JournalActa Crystallogr D Biol Crystallogr
Year2000
Volume56
Pages520-3
AuthorsWurtele M, Hahn M, Hilpert K, Hohne W
TitleAtomic resolution structure of native porcine pancreatic elastase at 1.1 A.
Related PDB1qnj
[45]
CommentsX-ray crystallography
PubMed ID11023818
JournalBiochem J
Year2000
Volume351 Pt 2
Pages335-40
AuthorsWright PA, Wilmouth RC, Clifton IJ, Schofield CJ
Title'pH-jump' crystallographic analyses of gamma-lactam-porcine pancreatic elastase complexes.
Related PDB1e34,1e35,1e36,1e37,1e38
[46]
CommentsX-ray crystallography
PubMed ID10738204
JournalBiopolymers
Year2000
Volume53
Pages434-45
AuthorsNakanishi I, Kinoshita T, Sato A, Tada T
TitleStructure of porcine pancreatic elastase complexed with FR901277, a novel macrocyclic inhibitor of elastases, at 1.6 A resolution.
Related PDB1qr3
[47]
CommentsX-ray crystallography
PubMed ID11310599
JournalBioorg Med Chem
Year2001
Volume9
Pages647-51
AuthorsOdagaki Y, Ohmoto K, Matsuoka S, Hamanaka N, Nakai H, Toda M, Katsuya Y
TitleThe crystal structure of the complex of non-peptidic inhibitor of human neutrophil elastase ONO-6818 and porcine pancreatic elastase.
Related PDB1fzz
[48]
CommentsX-ray crystallography
PubMed ID11294386
JournalBioorg Med Chem Lett
Year2001
Volume11
Pages895-8
AuthorsMacdonald SJ, Dowle MD, Harrison LA, Shah P, Johnson MR, Inglis GG, Clarke GD, Smith RA, Humphreys D, Molloy CR, Amour A, Dixon M, Murkitt G, Godward RE, Padfield T, Skarzynski T, Singh OM, Kumar KA, Fleetwood G, Hodgson ST, Hardy GW, Finch H
TitleThe discovery of a potent, intracellular, orally bioavailable, long duration inhibitor of human neutrophil elastase--GW311616A a development candidate.
Related PDB1hv7
[49]
CommentsX-ray crystallography
PubMed ID11358514
JournalEur J Biochem
Year2001
Volume268
Pages2969-74
AuthorsWright PA, Wilmouth RC, Clifton IJ, Schofield CJ
TitleKinetic and crystallographic analysis of complexes formed between elastase and peptides from beta-casein.
Related PDB1h9l
[50]
CommentsX-ray crystallography
PubMed ID11473259
JournalNat Struct Biol
Year2001
Volume8
Pages689-94
AuthorsWilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J
TitleX-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate.
Related PDB1hax,1hay,1haz,1hb0
[51]
PubMed ID11730873
JournalPhytochemistry
Year2001
Volume58
Pages1087-95
AuthorsMatern U, Oberer L, Falchetto RA, Erhard M, Konig WA, Herdman M, Weckesser J
TitleScyptolin A and B, cyclic depsipeptides from axenic cultures of Scytonema hofmanni PCC 7110.
[52]
CommentsX-ray crystallography
PubMed ID12037300
JournalActa Crystallogr D Biol Crystallogr
Year2002
Volume58
Pages976-91
AuthorsEvans G, Bricogne G
TitleTriiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment.
Related PDB1gwa
[53]
PubMed ID11914493
JournalActa Crystallogr D Biol Crystallogr
Year2002
Volume58
Pages672-4
AuthorsHilpert K, Schneider-Mergener J, Ay J
TitleCrystallization and preliminary X-ray analysis of the complex of porcine pancreatic elastase and a hybrid squash inhibitor.
[54]
CommentsX-ray crystallography
PubMed ID12198297
JournalActa Crystallogr D Biol Crystallogr
Year2002
Volume58
Pages1413-20
AuthorsPanjikar S, Tucker PA
TitleXenon derivatization of halide-soaked protein crystals.
Related PDB1l0z,1l1g,1lka,1lkb
[55]
PubMed ID12109112
JournalChem Commun (Camb)
Year2002
Volume-12
Pages1274-5
AuthorsMigaud ME, Wilmouth RC, Mills GI, Wayne GJ, Risley C, Chambers C, Macdonald SJ, Schofield CJ
Title5,5-Fused thiophene gamma-lactams as templates for serine protease inhibition.
[56]
CommentsX-ray crystallography
PubMed ID11896054
JournalJ Biol Chem
Year2002
Volume277
Pages21962-70
AuthorsKatona G, Wilmouth RC, Wright PA, Berglund GI, Hajdu J, Neutze R, Schofield CJ
TitleX-ray structure of a serine protease acyl-enzyme complex at 0.95-A resolution.
Related PDB1gvk
[57]
CommentsX-ray crystallography
PubMed ID12554935
JournalActa Crystallogr D Biol Crystallogr
Year2003
Volume59
Pages247-54
AuthorsAy J, Hilpert K, Krauss N, Schneider-Mergener J, Hohne W
TitleStructure of a hybrid squash inhibitor in complex with porcine pancreatic elastase at 1.8 A resolution.
Related PDB1mcv
[58]
CommentsX-ray crystallography
PubMed ID12467609
JournalBioorg Med Chem Lett
Year2003
Volume13
Pages21-4
AuthorsKinoshita T, Nakanishi I, Sato A, Tada T
TitleTrue interaction mode of porcine pancreatic elastase with FR136706, a potent peptidyl inhibitor.
Related PDB1mmj
[59]
CommentsX-ray crystallography
PubMed ID14583266
JournalChem Biol
Year2003
Volume10
Pages997-1001
AuthorsMatern U, Schleberger C, Jelakovic S, Weckesser J, Schulz GE
TitleBinding structure of elastase inhibitor scyptolin A.
Related PDB1okx
[60]
CommentsX-ray crystallography
JournalActa Crystallogr D Biol Crystallogr
Year2004
Volume60
Pages28-38
AuthorsMueller-Dieckmann C, Polentarutti M, Djinovic-Carugo K, Panjikar S, Tucker PA, Weiss MS
TitleOn the Routine Use of Soft X-Rays in Macromolecular Crystallography. Part II. Data-Collection Wavelength and Scaling Models.
Related PDB1uo6
[61]
PubMed ID14593107
JournalJ Biol Chem
Year2004
Volume279
Pages9264-9
AuthorsDobo J, Gettins PG
Titlealpha1-Proteinase inhibitor forms initial non-covalent and final covalent complexes with elastase analogously to other serpin-proteinase pairs, suggesting a common mechanism of inhibition.

comments
E.C. 3.4.21.11 was Transferred to EC 3.4.21.36 and 3.4.21.37.
This enzyme belongs to the peptidase family-S1.
Although this enzyme binds a calcium ion, it is not involved in catalysis.
As this enzyme has got a catalytic triad composed of Ser/His/Asp, it should have a similar mechanism to that of trypsin (D00197 in EzCatDB).

createdupdated
2004-03-252011-02-22


Copyright: Nozomi Nagano, JST & CBRC-AIST
Funded by PRESTO/Japan Science and Technology Corporation (JST) (December 2001 - November 2004)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (September 2005 - September 2008)
Funded by BIRD/Japan Science and Technology Corporation (JST) (October 2007 - September 2010)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)
Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2012 - March 2013)
Supported by the commission for the Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI) (October 2012 - )
© Biotechnology Research Institute for Drug Discovery, AIST, 2015-2016 All Rights Reserved.
© Computational Biology Research Center, AIST, 2004-2016 All Rights Reserved.