|
CATH domain | Related DB codes (homologues) |
---|
2.40.70.10 : Cathepsin D, subunit A; domain 1 | D00471,D00436,D00438,D00439,D00440,D00441,D00442,D00443,D00437,D00444,D00423,D00445,D00484,M00206,M00166,D00529 |
Enzyme Name | UniProtKB | KEGG |
---|
| P07267 |
---|
Protein name | Saccharopepsin | saccharopepsinyeast endopeptidase ASaccharomyces aspartic proteinaseaspartic proteinase yscAproteinase Aproteinase yscAyeast proteinase ASaccharomyces cerevisiae aspartic proteinase Ayeast proteinase APRA |
---|
Synonyms | EC 3.4.23.25Aspartate proteaseProteinase AProteinase YSCACarboxypeptidase Y-deficient protein 4 |
---|
RefSeq | NP_015171.1 (Protein) NM_001183968.1 (DNA/RNA sequence)
|
---|
MEROPS | A01.018 (Aspartic)
|
---|
Pfam | PF00026 (Asp) [Graphical view]
|
---|
UniProtKB:Accession Number | P07267 |
---|
Entry name | CARP_YEAST |
---|
Activity | Hydrolysis of proteins with broad specificity for peptide bonds. Cleaves -Leu-Leu-|-Val-Tyr- bond in a synthetic substrate. Does not act on esters of Tyr or Arg. |
---|
Subunit |
|
---|
Subcellular location | Vacuole. Note=Lysosome-like vacuoles. |
---|
Cofactor |
|
---|
Compound table: links to PDB-related databases & PoSSuM |
---|
| Substrates | Products | intermediates |
---|
KEGG-id | C00017 | C00012 | C00001 | C00017 | C00012 | I00136 |
---|
Compound | Protein | Peptide | H2O | Protein | Peptide | Amino-diol-tetrahedral intermediate |
---|
Type | peptide/protein | peptide/protein | H2O | peptide/protein | peptide/protein |
|
---|
ChEBI |
|
| 15377
|
|
|
|
---|
PubChem |
|
| 962 22247451
|
|
|
|
---|
| | | | | | | | | | | | | |
---|
1dp5A01 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1dpjA01 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fmuA01 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fmxA01 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fmxB01 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fq4A01 |  |  |  |  |  |  |  | Unbound | Analogue:2Y2 | | Unbound | Unbound | Unbound |
---|
1fq5A01 |  |  |  |  |  |  |  | Unbound | Analogue:0GM | | Unbound | Unbound | Unbound |
---|
1fq6A01 |  |  |  |  |  |  |  | Unbound | Analogue:0QF | | Unbound | Unbound | Unbound |
---|
1fq7A01 |  |  |  |  |  |  |  | Unbound | Analogue:2Y3 | | Unbound | Unbound | Unbound |
---|
1fq8A01 |  |  |  |  |  |  |  | Unbound | Analogue:2Y4 | | Unbound | Unbound | Unbound |
---|
1g0vA01 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1jxrA01 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Transition-state-analogue:MOR-PHE-NLE-CHF-NME(chain I) |
---|
2jxrA01 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Transition-state-analogue:2Z3 |
---|
1dp5A02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1dpjA02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fmuA02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fmxA02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fmxB02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fq4A02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fq5A02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fq6A02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fq7A02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1fq8A02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1g0vA02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
1jxrA02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
2jxrA02 |  |  |  |  |  |  |  | Unbound | Unbound | | Unbound | Unbound | Unbound |
---|
References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
---|
[3] |
|
|
references | [1] |
---|
PubMed ID | 1959673 |
---|
Journal | FEBS Lett |
---|
Year | 1991 |
---|
Volume | 293 |
---|
Pages | 62-6 |
---|
Authors | Rupp S, Hirsch HH, Wolf DH |
---|
Title | Biogenesis of the yeast vacuole (lysosome). Active site mutation in the vacuolar aspartate proteinase yscA blocks maturation of vacuolar proteinases. |
---|
[2] |
---|
PubMed ID | 8540315 |
---|
Journal | Adv Exp Med Biol |
---|
Year | 1995 |
---|
Volume | 362 |
---|
Pages | 155-66 |
---|
Authors | Aguilar CF, Dhanaraj V, Guruprasad K, Dealwis C, Badasso M, Cooper JB, Wood SP, Blundell TL |
---|
Title | Comparisons of the three-dimensional structures, specificities and glycosylation of renins, yeast proteinase A and cathepsin D. |
---|
[3] |
---|
Comments | X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) |
---|
Medline ID | 97280793 |
---|
PubMed ID | 9135120 |
---|
Journal | J Mol Biol |
---|
Year | 1997 |
---|
Volume | 267 |
---|
Pages | 899-915 |
---|
Authors | Aguilar CF, Cronin NB, Badasso M, Dreyer T, Newman MP, Cooper JB, Hoover DJ, Wood SP, Johnson MS, Blundell TL |
---|
Title | The three-dimensional structure at 2.4 A resolution of glycosylated proteinase A from the lysosome-like vacuole of Saccharomyces cerevisiae. |
---|
Related PDB | 1jxr,2jxr |
---|
Related UniProtKB | P07267 |
---|
[4] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 11061973 |
---|
Journal | J Mol Biol |
---|
Year | 2000 |
---|
Volume | 303 |
---|
Pages | 745-60 |
---|
Authors | Cronin NB, Badasso MO, J Tickle I, Dreyer T, Hoover DJ, Rosati RL, Humblet CC, Lunney EA, Cooper JB |
---|
Title | X-ray structures of five renin inhibitors bound to saccharopepsin: exploration of active-site specificity. |
---|
Related PDB | 1fq4,1fq5,1fq6,1fq7,1fq8 |
---|
[5] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 10655612 |
---|
Journal | Nat Struct Biol |
---|
Year | 2000 |
---|
Volume | 7 |
---|
Pages | 113-7 |
---|
Authors | Li M, Phylip LH, Lees WE, Winther JR, Dunn BM, Wlodawer A, Kay J, Gustchina A |
---|
Title | The aspartic proteinase from Saccharomyces cerevisiae folds its own inhibitor into a helix. |
---|
Related PDB | 1dp5,1dpj |
---|
[6] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 11042188 |
---|
Journal | J Biol Chem |
---|
Year | 2001 |
---|
Volume | 276 |
---|
Pages | 2023-30 |
---|
Authors | Phylip LH, Lees WE, Brownsey BG, Bur D, Dunn BM, Winther JR, Gustchina A, Li M, Copeland T, Wlodawer A, Kay J |
---|
Title | The potency and specificity of the interaction between the IA3 inhibitor and its target aspartic proteinase from Saccharomyces cerevisiae. |
---|
Related PDB | 1g0v |
---|
[7] |
---|
Comments | X-ray crystallography |
---|
PubMed ID | 12127998 |
---|
Journal | Biochem Biophys Res Commun |
---|
Year | 2002 |
---|
Volume | 295 |
---|
Pages | 1020-6 |
---|
Authors | Gustchina A, Li M, Phylip LH, Lees WE, Kay J, Wlodawer A |
---|
Title | An unusual orientation for Tyr75 in the active site of the aspartic proteinase from Saccharomyces cerevisiae. |
---|
Related PDB | 1fmu,1fmx |
---|
[8] |
---|
PubMed ID | 15065849 |
---|
Journal | Biochemistry |
---|
Year | 2004 |
---|
Volume | 43 |
---|
Pages | 4071-81 |
---|
Authors | Green TB, Ganesh O, Perry K, Smith L, Phylip LH, Logan TM, Hagen SJ, Dunn BM, Edison AS |
---|
Title | IA3, an aspartic proteinase inhibitor from Saccharomyces cerevisiae, is intrinsically unstructured in solution. |
---|
comments | This enzyme belongs to the peptidase family-A1. As it has a catalytic dyad composed of two aspartic acid residues, it must have a similar mechanism to that of pepsin (D00436 in EzCatDB).
|
created | updated |
---|
2004-03-25 | 2012-06-27 |
|
|