EzCatDB: D00452
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DB codeD00452
CATH domainDomain 11.10.10.10 : Arc Repressor Mutant, subunit ACatalytic domain
Domain 23.40.1360.10 : Dna Topoisomerase Vi A Subunit; Chain
E.C.5.99.1.3

CATH domainRelated DB codes (homologues)
1.10.10.10 : Arc Repressor Mutant, subunit AD00510,D00077,D00517,T00055,T00113

Enzyme Name
UniProtKBKEGG

Q57815
Protein nameType II DNA topoisomerase VI subunit ADNA topoisomerase (ATP-hydrolysing)
type II DNA topoisomerase
DNA-gyrase
deoxyribonucleate topoisomerase
deoxyribonucleic topoisomerase
topoisomerase
DNA topoisomerase II
SynonymsEC 5.99.1.3
RefSeqNP_247343.1 (Protein)
NC_000909.1 (DNA/RNA sequence)
PfamPF04406 (TP6A_N)
[Graphical view]


UniProtKB:Accession NumberQ57815
Entry nameTOP6A_METJA
ActivityATP-dependent breakage, passage and rejoining of double-stranded DNA.
SubunitHeterotetramer of two subunits A and two subunits B.
Subcellular location
CofactorBinds 1 magnesium ion per subunit.

Compound table: links to PDB-related databases & PoSSuM

CofactorsSubstratesProducts
KEGG-idC00305C00434C00002C00434C00008C00009
CompoundMagnesiumDouble-stranded DNAATPDouble-stranded DNAADPOrthophosphate
Typedivalent metal (Ca2+, Mg2+)nucleic acidsamine group,nucleotidenucleic acidsamine group,nucleotidephosphate group/phosphate ion
ChEBI18420

15422

16761
26078
PubChem888

5957

6022
22486802
1004
              
1d3yA01UnboundUnboundUnboundUnboundUnboundUnbound
1d3yB01UnboundUnboundUnboundUnboundUnboundUnbound
1d3yA02Bound:_MGUnboundUnboundUnboundUnboundUnbound
1d3yB02Bound:_MGUnboundUnboundUnboundUnboundUnbound

Active-site residues
resource
Swiss-prot;Q57815
pdbCatalytic residuesCofactor-binding residues
          
1d3yA01TYR 103
 
1d3yB01TYR 103
 
1d3yA02       
GLU 197;ASP 249(Magnesium binding)
1d3yB02       
GLU 197;ASP 249(Magnesium binding)


references
[1]
PubMed ID9519293
JournalCurr Opin Struct Biol
Year1998
Volume8
Pages26-32
AuthorsBerger JM
TitleType II DNA topoisomerases.
[2]
PubMed ID9801313
JournalNucleic Acids Res
Year1998
Volume26
Pages5157-62
AuthorsBuhler C, Gadelle D, Forterre P, Wang JC, Bergerat A
TitleReconstitution of DNA topoisomerase VI of the thermophilic archaeon Sulfolobus shibatae from subunits separately overexpressed in Escherichia coli.
[3]
CommentsX-ray crystallography
PubMed ID10545127
JournalEMBO J
Year1999
Volume18
Pages6177-88
AuthorsNichols MD, DeAngelis K, Keck JL, Berger JM
TitleStructure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11.
Related PDB1d3y
[4]
PubMed ID11809802
JournalMol Cell Biol
Year2002
Volume22
Pages1106-15
AuthorsDiaz RL, Alcid AD, Berger JM, Keeney S
TitleIdentification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation.
[5]
PubMed ID12596227
JournalBioessays
Year2003
Volume25
Pages232-42
AuthorsGadelle D, Filee J, Buhler C, Forterre P
TitlePhylogenomics of type II DNA topoisomerases.
[6]
PubMed ID12505993
JournalEMBO J
Year2003
Volume22
Pages151-63
AuthorsCorbett KD, Berger JM
TitleStructure of the topoisomerase VI-B subunit: implications for type II topoisomerase mechanism and evolution.

comments
Topoisomerase Type VI is an archaeal topoisomerase type II.

createdupdated
2004-04-222009-02-26


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