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CATH domain | Related DB codes (homologues) |
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3.40.50.720 : Rossmann fold | S00543,S00551,S00552,S00553,S00602,S00604,S00605,S00608,S00610,S00625,S00319,S00328,S00329,S00330,S00331,S00332,D00456,D00457,D00458,S00324,S00320,S00325,S00326,S00327,S00335,S00336,S00334,T00219,S00339,D00513,D00001,D00002,D00003,D00005,D00007,D00008,D00010,D00012,D00017,D00018,D00023,D00027,D00028,D00031,D00032,D00033,D00034,D00035,D00037,D00048,D00071,D00476,D00481,D00482,D00490,D00492,D00494,D00545,D00601,D00603,D00604,D00605,D00615,D00845,D00857,D00858,M00161,M00171,M00210,T00002,T00010,T00011,T00015,T00227,T00247,T00408,T00414,D00827,D00262,D00274,D00275,M00035,T00109 |
Enzyme Name | UniProtKB | KEGG |
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| P33160 |
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Protein name | Formate dehydrogenase | formate dehydrogenaseformate-NAD+ oxidoreductaseFDH IFDH IIN-FDHformic hydrogen-lyaseformate hydrogenlyasehydrogenlyaseNAD+-linked formate dehydrogenaseNAD+-dependent formate dehydrogenaseformate dehydrogenase (NAD+)NAD+-formate dehydrogenaseformate benzyl-viologen oxidoreductaseformic acid dehydrogenase |
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Synonyms | EC 1.2.1.2NAD-dependent formate dehydrogenaseFDH |
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Pfam | PF00389 (2-Hacid_dh) PF02826 (2-Hacid_dh_C) [Graphical view]
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KEGG pathways | MAP code | Pathways |
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MAP00630 | Glyoxylate and dicarboxylate metabolism | MAP00680 | Methane metabolism |
UniProtKB:Accession Number | P33160 |
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Entry name | FDH_PSESR |
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Activity | Formate + NAD(+) = CO(2) + NADH. |
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Subunit | Homodimer. |
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Subcellular location |
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Cofactor |
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References for Catalytic Mechanism | References | Sections | No. of steps in catalysis |
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[8] | p.451 |
| [11] |
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| [12] | Fig. 2, p.106 |
| [14] | Scheme 1, p.475 |
| [21] | p.148 |
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references | [1] |
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PubMed ID | 7144587 |
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Journal | Methods Enzymol |
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Year | 1982 |
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Volume | 89 Pt D |
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Pages | 531-7 |
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Authors | Hopner T, Ruschig U, Muller U, Willnow P |
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Title | Formate dehydrogenase from Pseudomonas oxalaticus. |
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[2] |
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PubMed ID | 6311213 |
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Journal | Biochem Biophys Res Commun |
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Year | 1983 |
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Volume | 115 |
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Pages | 61-7 |
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Authors | Durfor CN, Wetherbee PJ, Deaton JC, Solomon EI |
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Title | Characterization and spectroscopic properties of reduced Mo and W formate dehydrogenase from C. thermoaceticum. |
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[3] |
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PubMed ID | 6309816 |
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Journal | J Biol Chem |
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Year | 1983 |
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Volume | 258 |
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Pages | 10839-45 |
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Authors | Barber MJ, Siegel LM, Schauer NL, May HD, Ferry JG |
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Title | Formate dehydrogenase from Methanobacterium formicicum. Electron paramagnetic resonance spectroscopy of the molybdenum and iron-sulfur centers. |
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[4] |
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PubMed ID | 3038082 |
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Journal | Biochem J |
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Year | 1987 |
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Volume | 243 |
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Pages | 235-9 |
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Authors | Gadsby PM, Greenwood C, Coddington A, Thomson AJ, Godfrey C |
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Title | Purification and properties of formate dehydrogenase from Pseudomonas aeruginosa. Electron-paramagnetic-resonance studies on the molybdenum centre. |
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[5] |
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PubMed ID | 3038081 |
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Journal | Biochem J |
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Year | 1987 |
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Volume | 243 |
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Pages | 225-33 |
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Authors | Godfrey C, Coddington A, Greenwood C, Thomson AJ, Gadsby PM |
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Title | Purification and properties of formate dehydrogenase from Pseudomonas aeruginosa. Characterization of haem and iron-sulphur centres by magnetic-circular-dichroism and electron-paramagnetic-resonance spectroscopy. |
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[6] |
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PubMed ID | 3038083 |
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Journal | Biochem J |
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Year | 1987 |
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Volume | 243 |
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Pages | 241-8 |
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Authors | Godfrey C, Gadsby PM, Thomson AJ, Greenwood C, Coddington A |
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Title | Electron-paramagnetic-resonance and magnetic-circular-dichroism studies on the formate dehydrogenase-nitrate reductase particle from Pseudomonas aeruginosa. |
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[7] |
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PubMed ID | 1657982 |
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Journal | J Biol Chem |
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Year | 1991 |
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Volume | 266 |
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Pages | 21853-63 |
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Authors | Jollie DR, Lipscomb JD |
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Title | Formate dehydrogenase from Methylosinus trichosporium OB3b. Purification and spectroscopic characterization of the cofactors. |
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[8] |
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Comments | X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) |
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Medline ID | 92283270 |
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PubMed ID | 1597184 |
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Journal | Eur J Biochem |
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Year | 1992 |
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Volume | 206 |
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Pages | 441-52 |
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Authors | Lamzin VS, Aleshin AE, Strokopytov BV, Yukhnevich MG, Popov VO, Harutyunyan EH, Wilson KS |
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Title | Crystal structure of NAD-dependent formate dehydrogenase. |
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Related UniProtKB | P33160 |
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[9] |
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Comments | MUTAGENESIS OF CYS-255 |
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Medline ID | 93249485 |
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PubMed ID | 8484798 |
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Journal | Biochem Biophys Res Commun |
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Year | 1993 |
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Volume | 192 |
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Pages | 976-81 |
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Authors | Tishkov VI, Galkin AG, Marchenko GN, Egorova OA, Sheluho DV, Kulakova LB, Dementieva LA, Egorov AM |
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Title | Catalytic properties and stability of a Pseudomonas sp.101 formate dehydrogenase mutants containing Cys-255-Ser and Cys-255-Met replacements. |
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Related UniProtKB | P33160 |
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[10] |
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PubMed ID | 8509325 |
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Journal | J Bacteriol |
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Year | 1993 |
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Volume | 175 |
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Pages | 3703-9 |
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Authors | Chow CM, RajBhandary UL |
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Title | Developmental regulation of the gene for formate dehydrogenase in Neurospora crassa. |
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[11] |
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Comments | X-ray crystallography |
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PubMed ID | 8114093 |
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Journal | J Mol Biol |
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Year | 1994 |
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Volume | 236 |
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Pages | 759-85 |
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Authors | Lamzin VS, Dauter Z, Popov VO, Harutyunyan EH, Wilson KS |
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Title | High resolution structures of holo and apo formate dehydrogenase. |
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Related PDB | 2nac,2nad |
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[12] |
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PubMed ID | 8706817 |
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Journal | FEBS Lett |
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Year | 1996 |
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Volume | 390 |
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Pages | 104-8 |
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Authors | Tishkov VI, Matorin AD, Rojkova AM, Fedorchuk VV, Savitsky PA, Dementieva LA, Lamzin VS, Mezentzev AV, Popov VO |
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Title | Site-directed mutagenesis of the formate dehydrogenase active centre: role of the His332-Gln313 pair in enzyme catalysis. |
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[13] |
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PubMed ID | 9020054 |
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Journal | Biochem Biophys Res Commun |
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Year | 1997 |
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Volume | 230 |
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Pages | 30-4 |
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Authors | Duarte RO, Reis AR, Girio F, Moura I, Moura JJ, Collaco TA |
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Title | The formate dehydrogenase isolated from the aerobe Methylobacterium sp. RXM is a molybdenum-containing protein. |
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[14] |
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PubMed ID | 9020883 |
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Journal | Biochem J |
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Year | 1997 |
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Volume | 321 |
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Pages | 475-80 |
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Authors | Mesentsev AV, Lamzin VS, Tishkov VI, Ustinnikova TB, Popov VO |
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Title | Effect of pH on kinetic parameters of NAD+-dependent formate dehydrogenase. |
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[15] |
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PubMed ID | 9481877 |
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Journal | Biochemistry (Mosc) |
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Year | 1997 |
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Volume | 62 |
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Pages | 1439-43 |
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Authors | Klyachko NL, Vakula SV, Gladyshev VN, Tishkov VI, Levashov AV |
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Title | Formate dehydrogenase in a reversed micelle system: regulation of catalytic activity and oligomeric composition of the enzyme. |
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[16] |
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PubMed ID | 10069397 |
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Journal | FEBS Lett |
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Year | 1999 |
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Volume | 445 |
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Pages | 183-8 |
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Authors | Rojkova AM, Galkin AG, Kulakova LB, Serov AE, Savitsky PA, Fedorchuk VV, Tishkov VI |
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Title | Bacterial formate dehydrogenase. Increasing the enzyme thermal stability by hydrophobization of alpha-helices. |
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[17] |
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PubMed ID | 10691964 |
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Journal | Eur J Biochem |
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Year | 2000 |
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Volume | 267 |
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Pages | 1280-9 |
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Authors | Slusarczyk H, Felber S, Kula MR, Pohl M |
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Title | Stabilization of NAD-dependent formate dehydrogenase from Candida boidinii by site-directed mutagenesis of cysteine residues. |
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[18] |
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PubMed ID | 11171126 |
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Journal | Biochem J |
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Year | 2001 |
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Volume | 354 |
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Pages | 455-63 |
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Authors | Labrou NE, Rigden DJ |
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Title | Active-site characterization of Candida boidinii formate dehydrogenase. |
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[19] |
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PubMed ID | 12144528 |
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Journal | Biochem J |
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Year | 2002 |
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Volume | 367 |
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Pages | 841-7 |
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Authors | Serov AE, Popova AS, Fedorchuk VV, Tishkov VI |
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Title | Engineering of coenzyme specificity of formate dehydrogenase from Saccharomyces cerevisiae. |
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[20] |
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PubMed ID | 12460112 |
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Journal | Biochemistry (Mosc) |
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Year | 2002 |
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Volume | 67 |
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Pages | 1145-51 |
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Authors | Fedorchuk VV, Galkin AG, Yasny IE, Kulakova LB, Rojkova AM, Filippova AA, Tishkov VI |
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Title | Effect of interactions between amino acid residues 43 and 61 on thermal stability of bacterial formate dehydrogenases. |
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[21] |
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PubMed ID | 11825616 |
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Journal | Biochim Biophys Acta |
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Year | 2002 |
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Volume | 1594 |
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Pages | 136-49 |
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Authors | Galkin AG, Kutsenko AS, Bajulina NP, Esipova NG, Lamzin VS, Mesentsev AV, Shelukho DV, Tikhonova TV, Tishkov VI, Ustinnikova TB, Popov VO |
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Title | Site-directed mutagenesis of the essential arginine of the formate dehydrogenase active centre. |
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created | updated |
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2005-01-14 | 2009-02-26 |
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